FastQCFastQC Report
Thu 26 May 2016
SRR937684_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937684_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences553745
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT46990.8485855402757586No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29820.5385150204516519No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26320.47530903213573034No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16980.30663933760124246No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT14050.2537268959539138No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT10000.18058853804549024No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA9780.17661559020848946No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT9590.17318440798562515No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC9460.1708367569910338No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8990.16234909570289574No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC8860.16000144470830435No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG7960.14374847628421022No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC7730.13959493990916397No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7290.13164904423516238No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA6460.11666019557738672No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGTCG401.0760494E-535.6323538-39
TAGTCGG401.0760494E-535.6323540-41
ATTCCGT600.00395044931.664626
GAGTACG650.00584333729.228886
GAGTACT25500.022.91235412-13
GTACTTT26150.022.34081314-15
GTACATG19550.022.126531
TAGGACA2606.220944E-1021.9256214
TACATGG19650.021.7582472
CTAGGAC2858.367351E-1121.669183
AGTACCG1100.002873568621.5934125
GTACCGC1353.794173E-421.1097476
ACATGGG19150.020.8379273
TATAGGG1150.003718376220.654575
CAGGCCG1404.8435805E-420.3595035
ACTTTTT29400.020.03271916-17
TAGGATA1908.2303195E-620.002324
AGTACTT26700.019.83665312-13
AAGGTCG600.004765173419.79038878-79
TAGTACT1707.9372825E-519.5610924
Produced by FastQC (version 0.11.5)