FastQCFastQC Report
Thu 26 May 2016
SRR937683_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937683_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913962
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39190.4287924443248188No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31710.34695096732686914No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18500.2024154177088325No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10520.11510325374577937No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA27650.041.4399571
GTATCAA41600.035.200981
ATCAACG51600.028.098043
TCAACGC51850.027.9640984
TATCAAC52050.027.855122
CAACGCA52700.027.5130635
AACGCAG53700.026.9770436
TACATGG34900.026.4242232
TATAACG908.906247E-426.4090882
GTACATG36200.025.7421531
CGCAGAG60800.023.4387288
ACATGGG37900.023.329193
ACGCAGA61400.023.2870527
GAGTACT34500.023.06280312-13
TATAGGG1503.0144656E-522.1836342
GTACTTT36600.021.47995214-15
GTATAGG3101.0913936E-1121.4715631
GCAGAGT65200.021.2012659
CATGGGG17950.020.1279184
CATGGGA19150.020.1078644
Produced by FastQC (version 0.11.5)