##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937683_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 913962 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78687516548828 33.0 31.0 34.0 30.0 34.0 2 31.9306141830842 33.0 31.0 34.0 30.0 34.0 3 31.94868167385515 34.0 31.0 34.0 30.0 34.0 4 35.534067061869095 37.0 35.0 37.0 33.0 37.0 5 35.51551158582085 37.0 35.0 37.0 33.0 37.0 6 35.58031077878511 37.0 35.0 37.0 33.0 37.0 7 35.52732279897851 37.0 35.0 37.0 33.0 37.0 8 35.51455530973936 37.0 35.0 37.0 33.0 37.0 9 37.168603289852314 39.0 37.0 39.0 34.0 39.0 10-11 37.03222562863664 39.0 37.0 39.0 33.0 39.0 12-13 36.91596915407862 39.0 37.0 39.0 33.0 39.0 14-15 38.08858902230071 40.0 38.0 41.0 33.0 41.0 16-17 37.998563397602965 40.0 38.0 41.0 33.0 41.0 18-19 37.92327033290224 40.0 38.0 41.0 33.0 41.0 20-21 37.82487893369746 40.0 38.0 41.0 33.0 41.0 22-23 37.745276608874335 40.0 38.0 41.0 32.5 41.0 24-25 37.77417058914922 40.0 38.0 41.0 33.0 41.0 26-27 37.67714467341093 40.0 37.5 41.0 32.5 41.0 28-29 37.55206562198428 40.0 37.0 41.0 32.0 41.0 30-31 37.30980281455903 40.0 37.0 41.0 32.0 41.0 32-33 37.18470242745322 39.5 36.5 41.0 31.5 41.0 34-35 37.2005701549955 40.0 37.0 41.0 31.5 41.0 36-37 37.07902899682919 40.0 36.5 41.0 31.0 41.0 38-39 36.93787050227471 40.0 36.0 41.0 31.0 41.0 40-41 36.708637229994245 39.5 36.0 41.0 30.5 41.0 42-43 36.44566021344432 39.0 36.0 41.0 30.0 41.0 44-45 36.196480816489085 39.0 35.5 41.0 30.0 41.0 46-47 35.846855777373676 39.0 35.0 40.5 28.5 41.0 48-49 35.51317833783024 39.0 35.0 40.0 27.0 41.0 50-51 34.64022081880866 38.0 34.0 39.5 25.0 40.5 52-53 34.753049360914346 38.0 34.5 40.0 26.0 40.5 54-55 35.0364336810502 39.0 35.0 40.0 26.0 41.0 56-57 34.80755819169725 38.0 34.0 40.0 25.0 41.0 58-59 34.5558420371963 38.0 34.0 40.0 24.5 41.0 60-61 34.22545357465627 38.0 34.0 40.0 23.0 41.0 62-63 33.8551455093319 37.5 33.5 40.0 22.0 41.0 64-65 33.474885717349295 37.0 33.0 40.0 21.5 41.0 66-67 33.003516557581165 36.5 32.5 39.0 19.5 41.0 68-69 32.49789269138104 36.0 32.0 39.0 18.0 40.0 70-71 32.043340423343636 35.5 31.0 39.0 14.5 40.0 72-73 31.562924935610013 35.0 31.0 38.0 10.0 40.0 74-75 31.012036605460622 35.0 30.5 37.0 7.5 39.0 76-77 30.511087441272174 34.5 30.0 37.0 4.5 39.0 78-79 29.921248914068634 34.0 29.0 36.0 2.0 39.0 80-81 29.343661443254753 34.0 29.0 36.0 2.0 37.5 82-83 28.829690949951967 34.0 28.5 35.0 2.0 37.0 84-85 28.289765876480644 34.0 27.5 35.0 2.0 36.5 86-87 27.7397785684744 33.0 26.0 35.0 2.0 36.0 88-89 27.25828973195822 33.0 26.0 35.0 2.0 36.0 90-91 26.683183764751707 32.5 25.0 35.0 2.0 35.0 92-93 26.021271125057716 32.0 23.5 34.5 2.0 35.0 94-95 25.246358710756027 32.0 19.5 34.0 2.0 35.0 96-97 24.408326604388364 31.0 12.5 34.0 2.0 35.0 98-99 23.34456137126051 31.0 2.0 34.0 2.0 35.0 100-101 20.576143756523795 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3668642756066518 1101 2 0.3889915968892801 1101 3 0.4192984721170383 1101 4 0.3067809304383786 1101 5 0.23382096121437002 1101 6 0.2900590092633166 1101 7 0.21214027882865594 1101 8 0.2369233051846109 1101 9 0.2295264139335984 1101 10-11 0.39739531265053785 1101 12-13 0.2745499210175453 1101 14-15 0.40128409231011375 1101 16-17 0.36954053518228136 1101 18-19 0.3972661976856031 1101 20-21 0.35278198068899513 1101 22-23 0.3496710608579079 1101 24-25 0.18977070545447106 1101 26-27 0.1484734427294967 1101 28-29 0.33504528908832043 1101 30-31 0.5189796066052139 1101 32-33 0.34883797175429265 1101 34-35 0.430989810935273 1101 36-37 0.434226846376923 1101 38-39 0.4539833738818473 1101 40-41 0.3596945965831182 1101 42-43 0.5096225046265772 1101 44-45 0.4860779464284519 1101 46-47 0.6085500161226847 1101 48-49 0.7828172444724544 1101 50-51 0.8695050890969185 1101 52-53 0.7638911907896926 1101 54-55 0.9356923369499555 1101 56-57 0.7745955444130601 1101 58-59 0.6526417699079801 1101 60-61 0.7595370306971816 1101 62-63 0.8273529279581453 1101 64-65 0.7514992408002215 1101 66-67 0.8344528403266551 1101 68-69 0.9551605728841466 1101 70-71 1.0551099985219636 1101 72-73 0.9107330369398348 1101 74-75 1.1143500369303503 1101 76-77 1.0559047782663882 1101 78-79 1.0097483898514987 1101 80-81 1.082436023854278 1101 82-83 1.2017552677891707 1101 84-85 1.2335604361291956 1101 86-87 1.0493275230650525 1101 88-89 0.9447137249058564 1101 90-91 1.1233388239112152 1101 92-93 1.134415114108215 1101 94-95 1.0702117785677814 1101 96-97 1.2754301558063723 1101 98-99 1.5028287815774242 1101 100-101 1.5568107414487358 1102 1 0.1916906271999217 1102 2 0.19079968854699914 1102 3 0.21812410763440582 1102 4 0.13839926198151886 1102 5 0.10525311302246365 1102 6 0.05404755204884992 1102 7 0.015158538764211471 1102 8 0.2471059045401418 1102 9 0.2536975560746697 1102 10-11 0.16086291021587584 1102 12-13 0.1651603757257405 1102 14-15 0.27113729674977805 1102 16-17 0.3809601556620521 1102 18-19 0.2817434622756494 1102 20-21 0.3842875302772413 1102 22-23 0.2890659767189945 1102 24-25 0.2668795489918878 1102 26-27 0.27046592393250535 1102 28-29 0.3727896499905725 1102 30-31 0.49606875805527295 1102 32-33 0.4934978356998698 1102 34-35 0.454563029696466 1102 36-37 0.645974071582792 1102 38-39 0.5679647559083421 1102 40-41 0.5260891544062432 1102 42-43 0.5473274813541167 1102 44-45 0.7193128193249763 1102 46-47 0.7146044378913885 1102 48-49 0.7780231162948681 1102 50-51 0.8330210217857896 1102 52-53 0.6987891499733649 1102 54-55 0.6487231282138239 1102 56-57 0.7638973704066103 1102 58-59 0.6814960484615042 1102 60-61 0.6749326626341698 1102 62-63 0.5441252158206638 1102 64-65 0.5382536267651332 1102 66-67 0.560095518450531 1102 68-69 0.5298669817634831 1102 70-71 0.5758941016368908 1102 72-73 0.587494583663787 1102 74-75 0.6346973337438122 1102 76-77 0.6176161996770588 1102 78-79 1.033011905784189 1102 80-81 0.9754238505639101 1102 82-83 1.070887025755514 1102 84-85 1.177001180848137 1102 86-87 1.2416547697532003 1102 88-89 1.1906285118732747 1102 90-91 1.400096790255649 1102 92-93 1.5477735100981889 1102 94-95 1.4759117427639836 1102 96-97 1.6702672485382024 1102 98-99 1.4370786920679315 1102 100-101 1.9561626927555729 1103 1 0.10475640369639194 1103 2 0.2978287455229456 1103 3 0.18849888951741534 1103 4 0.17123667947640087 1103 5 0.11631297191025425 1103 6 0.03058271630042242 1103 7 0.1746525073553613 1103 8 0.13825376889034402 1103 9 0.20595307538276586 1103 10-11 0.19843555265574508 1103 12-13 0.18943774466588081 1103 14-15 0.2931822857491042 1103 16-17 0.23533653074740357 1103 18-19 0.37806088887454337 1103 20-21 0.29141597273229536 1103 22-23 0.29978519377384316 1103 24-25 0.26343861127851653 1103 26-27 0.23315843588152063 1103 28-29 0.34007855068095694 1103 30-31 0.24098606326731442 1103 32-33 0.23914156531066766 1103 34-35 0.40216146684084464 1103 36-37 0.23726115147051274 1103 38-39 0.2527572602706769 1103 40-41 0.4385669633736953 1103 42-43 0.6275968084157881 1103 44-45 0.5364757811639933 1103 46-47 0.7926888017484615 1103 48-49 0.7546394579988345 1103 50-51 0.6911577337196277 1103 52-53 0.5565491833547469 1103 54-55 0.6910158222431093 1103 56-57 0.6351218602025313 1103 58-59 0.7546407264070396 1103 60-61 0.8394023538563786 1103 62-63 0.6922179250233 1103 64-65 0.7609673814119731 1103 66-67 0.6884230353308922 1103 68-69 0.5978788928476249 1103 70-71 0.6927934264225684 1103 72-73 0.6498692138132434 1103 74-75 0.49776652424528933 1103 76-77 0.6596695340219441 1103 78-79 0.6912719012321809 1103 80-81 0.7328925555180099 1103 82-83 1.0632419305427057 1103 84-85 0.8918399639450172 1103 86-87 0.955658638958873 1103 88-89 0.9092477365148035 1103 90-91 1.0272878228024958 1103 92-93 0.8691702174800291 1103 94-95 0.9633849997247594 1103 96-97 1.4959284275079305 1103 98-99 1.3932432471092824 1103 100-101 1.4686006716646105 1104 1 0.3463193499557562 1104 2 0.32646878625817166 1104 3 0.29525561523229626 1104 4 0.20659573521550811 1104 5 0.222731832625648 1104 6 0.23275328356906044 1104 7 0.12754761797982894 1104 8 0.10438495622260291 1104 9 0.23818903340710307 1104 10-11 0.22394914573995095 1104 12-13 0.49271034525011004 1104 14-15 0.429007365477581 1104 16-17 0.37486376860053383 1104 18-19 0.19205137122707328 1104 20-21 0.3323368797801791 1104 22-23 0.39232287577109304 1104 24-25 0.28569415207308424 1104 26-27 0.3799995417050468 1104 28-29 0.3079110270686911 1104 30-31 0.32620222029701296 1104 32-33 0.4272022010711467 1104 34-35 0.4337614155389602 1104 36-37 0.4690245770579011 1104 38-39 0.6387838147368186 1104 40-41 0.5248507929442567 1104 42-43 0.5742935798429372 1104 44-45 0.5159689070947948 1104 46-47 0.567750153438169 1104 48-49 0.5605116091173272 1104 50-51 0.5328129467947136 1104 52-53 0.6425214688535448 1104 54-55 0.7608974067821066 1104 56-57 0.6405286573189102 1104 58-59 0.7265491180930681 1104 60-61 0.7703108757113668 1104 62-63 0.7257986431730572 1104 64-65 0.7421053260442321 1104 66-67 0.7231767097324067 1104 68-69 0.6885937309595604 1104 70-71 0.6854991795073815 1104 72-73 0.5992947476016575 1104 74-75 0.6464285597048445 1104 76-77 0.6927884395307728 1104 78-79 0.5697213784507014 1104 80-81 0.762543798440074 1104 82-83 0.7164096302659075 1104 84-85 0.8624897367236244 1104 86-87 0.6849057838560597 1104 88-89 0.8200150059147617 1104 90-91 0.9675828660453867 1104 92-93 1.39578881096622 1104 94-95 1.4696661302038194 1104 96-97 1.6220812841421441 1104 98-99 1.5763359274028517 1104 100-101 1.5538503280568605 1105 1 0.10710189767644351 1105 2 0.134745598214149 1105 3 0.005139444828966333 1105 4 -0.01726655123779608 1105 5 0.05970189375278068 1105 6 0.03201469018303271 1105 7 0.10443145133971399 1105 8 0.015742460258650226 1105 9 0.11966787599313733 1105 10-11 0.16922951142395704 1105 12-13 0.05216974707476396 1105 14-15 0.17896001170665699 1105 16-17 0.327676206272308 1105 18-19 0.2515672212554847 1105 20-21 0.3341306782550717 1105 22-23 0.58419784676299 1105 24-25 0.32666676716782916 1105 26-27 0.35224772313208064 1105 28-29 0.2902283136411725 1105 30-31 0.016636507688616575 1105 32-33 0.17324925880260622 1105 34-35 0.26698143432994215 1105 36-37 0.19840937944528036 1105 38-39 0.3578799911863726 1105 40-41 0.366840353789776 1105 42-43 0.15197885225569507 1105 44-45 0.2450181562709659 1105 46-47 0.3562763661023851 1105 48-49 0.5194787826052405 1105 50-51 0.2917671999159879 1105 52-53 0.43581567312829605 1105 54-55 0.5883597087900228 1105 56-57 0.5623447591063098 1105 58-59 0.5530210677262133 1105 60-61 0.6436492601135981 1105 62-63 0.5776045466516493 1105 64-65 0.550360813123234 1105 66-67 0.24448339784102302 1105 68-69 0.522175018254579 1105 70-71 0.43484908436079195 1105 72-73 0.6503333318305344 1105 74-75 0.3668224176385131 1105 76-77 0.5648711650005822 1105 78-79 0.5302427591486776 1105 80-81 0.3877477959764235 1105 82-83 0.5530347301478677 1105 84-85 0.5352218674175262 1105 86-87 0.6917266084765075 1105 88-89 0.541061781405201 1105 90-91 0.5984467368116135 1105 92-93 0.7342986536513507 1105 94-95 0.9430583285602978 1105 96-97 1.1843580785364658 1105 98-99 1.2016321236720025 1105 100-101 1.2236408801328373 1106 1 0.010418814126865072 1106 2 0.029456890558236637 1106 3 -0.07742158890508222 1106 4 0.07701357727285796 1106 5 0.1127301908100975 1106 6 0.09928860499176295 1106 7 0.2083454657089021 1106 8 0.29571430442182844 1106 9 0.09573541469637803 1106 10-11 0.06645519060629823 1106 12-13 0.09034892940808703 1106 14-15 0.09499118613162238 1106 16-17 0.04002108438136531 1106 18-19 0.2234389665643164 1106 20-21 0.26346085177976164 1106 22-23 0.3899170562812557 1106 24-25 0.1357851982080973 1106 26-27 0.15439449536107475 1106 28-29 0.1869045568228671 1106 30-31 0.17429043803160482 1106 32-33 0.39340318914559447 1106 34-35 0.4255168895012602 1106 36-37 0.3214598745996611 1106 38-39 0.3226046553463746 1106 40-41 0.3214699589019574 1106 42-43 0.36449473956174216 1106 44-45 0.23455239341548406 1106 46-47 0.20927465380384547 1106 48-49 0.5298805549224852 1106 50-51 0.5020279723921206 1106 52-53 0.4021158284708477 1106 54-55 0.41423924770509757 1106 56-57 0.5843972225213747 1106 58-59 0.6467969949270511 1106 60-61 0.3632460314599584 1106 62-63 0.4832549111770632 1106 64-65 0.5749927571999152 1106 66-67 0.7720291179773042 1106 68-69 0.6947486735706434 1106 70-71 0.5559776567980848 1106 72-73 0.907805882096202 1106 74-75 0.6424583511942679 1106 76-77 0.5243353656042942 1106 78-79 0.771743050415953 1106 80-81 0.4050142084461932 1106 82-83 0.47333230821632455 1106 84-85 0.6354387732764124 1106 86-87 0.8024644414479667 1106 88-89 1.0373624663246197 1106 90-91 0.8616111351240257 1106 92-93 0.8184826198290409 1106 94-95 0.9877270340979081 1106 96-97 1.1883210787659486 1106 98-99 1.4200770653302826 1106 100-101 0.9903633730276837 1107 1 0.16193192928489708 1107 2 0.11689501075624875 1107 3 0.2263214391172319 1107 4 0.2012967795666114 1107 5 0.1566564502460679 1107 6 0.18010754637115411 1107 7 0.2077434676352965 1107 8 0.23762289031435557 1107 9 0.3780025114416645 1107 10-11 0.46441651713004717 1107 12-13 0.4084181115867054 1107 14-15 0.33651281326241644 1107 16-17 0.652482724504118 1107 18-19 0.6423806259510059 1107 20-21 0.6296800798782272 1107 22-23 0.7249401842072416 1107 24-25 0.6383667899470069 1107 26-27 0.6332391630581355 1107 28-29 0.6468324850535012 1107 30-31 0.8187965756119695 1107 32-33 0.6508343653658315 1107 34-35 0.866557478171373 1107 36-37 0.6395374567513912 1107 38-39 0.6673717520916611 1107 40-41 0.6866909901946556 1107 42-43 0.4815021651379823 1107 44-45 0.3436764442185236 1107 46-47 0.1011053999454603 1107 48-49 0.11042509717995586 1107 50-51 0.1319769565553699 1107 52-53 -0.012693044140753784 1107 54-55 0.22644384865037637 1107 56-57 0.17801212295368884 1107 58-59 0.26267322412311245 1107 60-61 0.202217146752389 1107 62-63 0.270834990920811 1107 64-65 0.561561200180897 1107 66-67 0.5959810813310185 1107 68-69 0.651004413552279 1107 70-71 0.432589051641628 1107 72-73 0.4509327440902453 1107 74-75 0.6845702164363026 1107 76-77 0.6173272680302091 1107 78-79 0.6719009699106131 1107 80-81 1.0005527027230094 1107 82-83 0.905184726288585 1107 84-85 0.9171052719436155 1107 86-87 0.5451774647662901 1107 88-89 0.6030992559668924 1107 90-91 0.6066999508398183 1107 92-93 0.6470713683450455 1107 94-95 0.8411573031210509 1107 96-97 1.4015235965178903 1107 98-99 1.8021400427287553 1107 100-101 1.786956623929278 1108 1 -0.050644686672463024 1108 2 0.009709530964975954 1108 3 0.11999959200536736 1108 4 -0.010843827659400063 1108 5 -0.0909545852467204 1108 6 0.06369880495507374 1108 7 0.2124305614973565 1108 8 0.030242045072299106 1108 9 0.033826721664951265 1108 10-11 0.012043483711096314 1108 12-13 0.42400821866038285 1108 14-15 0.061294878769935224 1108 16-17 0.002907972763573241 1108 18-19 0.28379977847301063 1108 20-21 0.06893232490395462 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-2.124371414263649 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3805.0 3 788.0 4 751.0 5 787.0 6 848.0 7 1021.0 8 1239.0 9 1535.0 10 2007.0 11 2529.0 12 3331.0 13 4134.0 14 4626.0 15 5964.0 16 7495.0 17 9835.0 18 8776.0 19 6232.0 20 6123.0 21 6546.0 22 7289.0 23 7794.0 24 8822.0 25 9921.0 26 11733.0 27 13664.0 28 16333.0 29 19233.0 30 23581.0 31 29296.0 32 37100.0 33 48611.0 34 65382.0 35 91925.0 36 132097.0 37 175104.0 38 124573.0 39 13127.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.08325113802558 19.067263373514862 15.305004021078572 22.54448146738099 2 14.826355869832986 19.776225402288638 41.912054235890146 23.48536449198823 3 19.023296115120537 24.48483120187773 30.155855836093625 26.336016846908112 4 12.526228387972838 16.619776396479992 37.82185645983396 33.032138755713206 5 13.404598407231083 37.18555977271231 34.815164213158994 14.59467760689761 6 31.303609202205195 36.1878144214645 17.921064457085237 14.587511919245062 7 27.796629861261817 33.05219615812076 21.161682921108415 17.989491059509014 8 25.486546800124515 35.40239031597592 20.21509336349726 18.895969520402303 9 25.806147546374035 17.386372105870027 21.09739095361062 35.710089394145314 10-11 24.453598483810467 26.972597439183065 27.58178073347532 20.992023343531148 12-13 25.11530283434534 24.33306297814507 28.51849010280804 22.03314408470155 14-15 22.2870467542218 25.660540730125415 26.89632306802831 25.156089447624474 16-17 21.342014474048376 29.12007541192944 27.589585919452826 21.948324194569356 18-19 21.21125102616347 27.591165513454385 30.18329005818854 21.01429340219361 20-21 21.974553357613114 26.486351117195063 30.457881740735342 21.081213784456484 22-23 21.81098237261684 26.320628992025814 30.417044772062724 21.451343863294625 24-25 21.501549192195185 26.552562386906775 30.439297641084995 21.506590779813045 26-27 21.26808584323155 26.679372330002387 30.442959316305263 21.6095825104608 28-29 21.198531962478125 26.867859634393554 30.561627928005443 21.37198047512288 30-31 21.491001640848243 26.587167185665763 30.457902980600288 21.46392819288571 32-33 21.271884875116598 26.5803200811986 30.464929943189478 21.682865100495324 34-35 21.52073346307447 26.752560341411737 30.229538779574703 21.49716741593909 36-37 21.50568862094984 26.53522627083677 30.378161920349996 21.580923187863398 38-39 21.272182038048975 26.846382246369867 30.29271769998833 21.588718015592832 40-41 21.519151032360124 26.906733340895006 30.186252769188705 21.38786285755617 42-43 21.258428936631933 26.651578379178165 30.175880842715564 21.91411184147434 44-45 21.4683057896303 26.8186543489495 30.025038401531788 21.688001459888415 46-47 21.58179588266821 27.16719988338282 29.501406749548163 21.749597484400805 48-49 21.65737978382241 27.115578946906943 29.432166664109303 21.794874605161343 50-51 21.603431901492854 27.328346013532734 29.31068781062287 21.757534274351546 52-53 21.623151896638856 27.55555409391824 28.921070488555927 21.90022352088698 54-55 21.63197671360185 27.345462396324677 29.077201949885144 21.945358940188324 56-57 21.642971542289864 27.68080374387896 28.986514956502774 21.689709757328394 58-59 21.758319671771854 27.26681467909211 29.209916101940088 21.764949547195947 60-61 21.685589821108938 27.326046782004493 29.422596783127663 21.565766613758903 62-63 21.375058422832822 27.44122512337971 29.445107741840392 21.73860871194708 64-65 21.475504689099242 27.361261180307316 29.35453929791725 21.808694832676196 66-67 21.473546599866093 27.32856875166426 29.143394402821933 22.054490245647717 68-69 21.251026750023154 27.415746221916095 29.290664369596037 22.04256265846472 70-71 21.542979002265138 27.26889176977777 29.17519152857943 22.012937699377662 72-73 21.478963915441437 27.365539021928637 29.0121867161397 22.143310346490225 74-75 21.386327075397677 27.774265024963068 28.750487645581384 22.08892025405787 76-77 21.414340883089103 27.440757722536613 28.804776641491074 22.340124752883213 78-79 21.610236127709403 27.33477005589629 28.855164447746752 22.19982936864756 80-81 21.424054933108902 27.61772789437694 28.745693282836253 22.212523889677907 82-83 21.55952545329818 27.476620049225563 28.782379615444466 22.181474882031793 84-85 21.49870149793445 27.496082578156678 28.778586221632935 22.22662970227594 86-87 21.39392996381822 27.455090542113098 28.884884518536865 22.266094975531825 88-89 21.434418190803566 27.343624156661296 28.748412490562608 22.473545161972535 90-91 21.520636632213836 27.232639893397852 28.83797306893196 22.408750405456345 92-93 21.55269049082679 27.179452620690487 28.912955578712758 22.354901309769964 94-95 21.360556890312225 27.225766861081507 28.903067444326037 22.51060880428023 96-97 21.26515628852423 27.300057529518124 28.801632742514315 22.633153439443333 98-99 21.34199527928694 27.280856127831825 28.68046865240517 22.69667994047606 100-101 21.57823895450215 26.68230501685359 29.166710035514974 22.572745993129278 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 941.0 1 541.5 2 156.0 3 166.0 4 152.0 5 158.5 6 245.5 7 482.5 8 852.5 9 1116.0 10 1123.5 11 1075.0 12 1132.5 13 1255.5 14 1371.5 15 1454.0 16 1516.0 17 1583.5 18 1659.5 19 1758.0 20 1877.0 21 2162.5 22 2628.5 23 3233.0 24 3921.5 25 4822.0 26 6203.0 27 7531.0 28 8502.5 29 9860.0 30 11638.5 31 13336.5 32 15002.5 33 16999.5 34 19211.0 35 21088.0 36 23755.0 37 26410.5 38 27830.5 39 28752.0 40 29325.0 41 30775.5 42 32327.5 43 33377.5 44 35068.0 45 36599.0 46 37754.5 47 38733.5 48 38206.5 49 36482.5 50 34274.5 51 31612.5 52 29549.5 53 28377.5 54 27142.5 55 24601.0 56 21244.5 57 18419.0 58 16247.0 59 13829.0 60 11130.0 61 8885.5 62 7028.0 63 5341.0 64 4041.5 65 3025.5 66 2119.0 67 1532.0 68 1146.0 69 792.5 70 554.5 71 399.5 72 283.0 73 212.5 74 150.5 75 101.0 76 72.0 77 46.5 78 26.5 79 20.5 80 20.0 81 16.5 82 9.5 83 7.0 84 4.0 85 4.0 86 5.0 87 2.0 88 0.0 89 1.5 90 2.0 91 1.0 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2751755543447102 2 0.24519618977594254 3 0.24311732872920316 4 0.24716563708337982 5 0.2562469774454518 6 0.17199839818285662 7 0.17462432792610635 8 0.17659377523354364 9 0.1748431554047105 10-11 0.18326801333097 12-13 0.17309253557587734 14-15 0.1733660699241325 16-17 0.17938382558574648 18-19 0.1732019493151794 20-21 0.16882539974309654 22-23 0.16609005626054477 24-25 0.1697007096575131 26-27 0.16499591886752402 28-29 0.17369431114203873 30-31 0.1782349813230747 32-33 0.17845380880167885 34-35 0.17861792941063195 36-37 0.1622058685153212 38-39 0.15618811285370726 40-41 0.160345834947186 42-43 0.17008365774507037 44-45 0.171068381398789 46-47 0.17232663940076284 48-49 0.17112308826844003 50-51 0.1730378287062263 52-53 0.19070814760351085 54-55 0.16390178147450332 56-57 0.15673518155021762 58-59 0.15602399224475416 60-61 0.14962328849558296 62-63 0.1568445952895197 64-65 0.15536750980894173 66-67 0.15263216632638993 68-69 0.16362824712624813 70-71 0.15700871589847282 72-73 0.15438278615522308 74-75 0.1547110273731293 76-77 0.15733695711637902 78-79 0.1606740761650922 80-81 0.15689930215917072 82-83 0.1562428197233583 84-85 0.15011565032244228 86-87 0.14748972057919257 88-89 0.14978740910453606 90-91 0.154273372415921 92-93 0.14869327171151536 94-95 0.1525774594567389 96-97 0.15175685641197337 98-99 0.15186627015127543 100-101 0.13660305351863644 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 913962.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.65603683928688 #Duplication Level Percentage of deduplicated Percentage of total 1 82.71072263882313 53.47747529939788 2 10.308790222066017 13.330510407327617 3 2.943384821905453 5.709227924319505 4 1.2751533209443822 3.2978544037887594 5 0.7051192312122151 2.2795107494673315 6 0.42488831883565764 1.6482956877132569 7 0.28111771152572873 1.2723169978808477 8 0.20054922888835106 1.0373374664876642 9 0.16213057035510528 0.9434448116688852 >10 0.9056875185328463 10.824427592909277 >50 0.058876418443007006 2.6332287473926286 >100 0.022392516638754793 2.2286423158441226 >500 5.08920784031273E-4 0.221778358474476 >1k 6.785610453750306E-4 1.0959492373278754 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3919 0.4287924443248188 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3171 0.34695096732686914 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1850 0.2024154177088325 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1052 0.11510325374577937 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.0941373930207163E-4 0.0 16-17 0.0 0.0 0.0 1.0941373930207163E-4 0.0 18-19 0.0 1.0941373930207163E-4 0.0 1.0941373930207163E-4 0.0 20-21 0.0 1.0941373930207163E-4 0.0 1.0941373930207163E-4 0.0 22-23 0.0 1.0941373930207163E-4 0.0 2.1882747860414327E-4 0.0 24-25 0.0 1.0941373930207163E-4 0.0 2.1882747860414327E-4 0.0 26-27 0.0 1.0941373930207163E-4 0.0 4.923618268593224E-4 0.0 28-29 0.0 1.0941373930207163E-4 0.0 0.0014770854805779671 0.0 30-31 0.0 1.0941373930207163E-4 0.0 0.0038841877452235433 0.0 32-33 0.0 1.0941373930207163E-4 0.0 0.007494841142191907 0.0 34-35 0.0 1.0941373930207163E-4 0.0 0.01137902888741545 0.0 36-37 0.0 1.0941373930207163E-4 0.0 0.015099096023685886 0.0 38-39 0.0 1.0941373930207163E-4 0.0 0.023359833340992293 0.0 40-41 0.0 1.0941373930207163E-4 0.0 0.03780244692886575 0.0 42-43 0.0 1.0941373930207163E-4 0.0 0.05005678573069777 0.0 44-45 0.0 1.0941373930207163E-4 0.0 0.06745357027972716 0.0 46-47 0.0 1.0941373930207163E-4 0.0 0.10137182946336937 0.0 48-49 0.0 1.0941373930207163E-4 0.0 0.12489578341331478 0.0 50-51 0.0 1.0941373930207163E-4 0.0 0.14984211597418712 0.0 52-53 0.0 1.0941373930207163E-4 0.0 0.1768673095817988 0.0 54-55 0.0 1.0941373930207163E-4 0.0 0.21461504964101352 0.0 56-57 0.0 1.0941373930207163E-4 0.0 0.29957481820907217 0.0 58-59 0.0 1.0941373930207163E-4 0.0 0.3797750891174907 0.0 60-61 0.0 1.0941373930207163E-4 0.0 0.44421978156641084 0.0 62-63 0.0 1.0941373930207163E-4 0.0 0.5231070876032045 0.0 64-65 0.0 1.0941373930207163E-4 0.0 0.6025961692061595 0.0 66-67 0.0 1.0941373930207163E-4 0.0 0.7160035099927569 0.0 68-69 0.0 1.0941373930207163E-4 0.0 0.8546854245581326 0.0 70-71 0.0 1.0941373930207163E-4 0.0 0.951461876970815 0.0 72-73 0.0 1.0941373930207163E-4 0.0 1.0554596361774342 0.0 74-75 0.0 1.0941373930207163E-4 0.0 1.2300292572338893 0.0 76-77 0.0 1.0941373930207163E-4 0.0 1.4419636702620022 0.0 78-79 0.0 1.0941373930207163E-4 0.0 1.652803945897094 0.0 80-81 0.0 1.0941373930207163E-4 0.0 1.8092655930990564 0.0 82-83 0.0 1.0941373930207163E-4 0.0 2.045106908164672 0.0 84-85 0.0 1.0941373930207163E-4 0.0 2.35764725448104 0.0 86-87 0.0 1.0941373930207163E-4 0.0 2.638949978226666 0.0 88-89 0.0 1.6412060895310744E-4 0.0 2.998592939312575 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2765 0.0 41.439957 1 GTATCAA 4160 0.0 35.20098 1 ATCAACG 5160 0.0 28.09804 3 TCAACGC 5185 0.0 27.964098 4 TATCAAC 5205 0.0 27.85512 2 CAACGCA 5270 0.0 27.513063 5 AACGCAG 5370 0.0 26.977043 6 TACATGG 3490 0.0 26.424223 2 TATAACG 90 8.906247E-4 26.409088 2 GTACATG 3620 0.0 25.742153 1 CGCAGAG 6080 0.0 23.438728 8 ACATGGG 3790 0.0 23.32919 3 ACGCAGA 6140 0.0 23.287052 7 GAGTACT 3450 0.0 23.062803 12-13 TATAGGG 150 3.0144656E-5 22.183634 2 GTACTTT 3660 0.0 21.479952 14-15 GTATAGG 310 1.0913936E-11 21.471563 1 GCAGAGT 6520 0.0 21.201265 9 CATGGGG 1795 0.0 20.127918 4 CATGGGA 1915 0.0 20.107864 4 >>END_MODULE