FastQCFastQC Report
Thu 26 May 2016
SRR937683_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937683_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences913962
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51670.5653407909738042No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44850.49072062076979134No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28310.3097502959641648No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11860.12976469481225694No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10230.11193025530601929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32650.044.098711
GTATCAA48850.034.4354021
CGCCCGA452.413942E-531.66627334-35
GGACCGA2300.028.9118946
TATCAAC62000.027.04922
ATCAACG61900.026.9393983
TCAACGC62250.026.787934
CAACGCA64000.026.0554495
AACGCAG65500.025.4531866
GAGTACT44900.024.06773212-13
GTACTTT46300.023.2361214-15
ACCGAGT2503.8198777E-1022.8003438
TACATGG35900.022.62942
GTACATG36550.022.361841
ACGCAGA75650.021.8509547
CGCAGAG75250.021.7777358
CGCGGAA550.002878672221.59005282-83
ACATGGG35950.021.5407143
AGGACCG2709.986252E-1021.1148935
TACACGG1353.790537E-421.1148935
Produced by FastQC (version 0.11.5)