##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937683_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 913962 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.288868683818365 33.0 31.0 34.0 31.0 34.0 2 32.58990417544712 34.0 31.0 34.0 31.0 34.0 3 32.68359734868627 34.0 31.0 34.0 31.0 34.0 4 36.27894485766367 37.0 35.0 37.0 35.0 37.0 5 36.20631164096538 37.0 35.0 37.0 35.0 37.0 6 36.253780791761585 37.0 37.0 37.0 35.0 37.0 7 36.205306128701196 37.0 36.0 37.0 35.0 37.0 8 36.18306997446283 37.0 36.0 37.0 35.0 37.0 9 37.91036717062635 39.0 38.0 39.0 35.0 39.0 10-11 37.834181289812925 39.0 38.0 39.0 35.0 39.0 12-13 37.73412570763336 39.0 37.0 39.0 35.0 39.0 14-15 39.00915027101783 40.0 38.0 41.0 36.0 41.0 16-17 38.959207275576006 40.0 38.0 41.0 36.0 41.0 18-19 38.93533483886639 40.0 38.0 41.0 35.5 41.0 20-21 38.84251752261035 40.0 38.0 41.0 35.0 41.0 22-23 38.78211840317212 40.0 38.0 41.0 35.0 41.0 24-25 38.815920136723406 40.0 38.0 41.0 35.0 41.0 26-27 38.685871513257666 40.0 38.0 41.0 35.0 41.0 28-29 38.52374989332161 40.0 38.0 41.0 34.0 41.0 30-31 38.58870773620785 40.0 38.0 41.0 35.0 41.0 32-33 38.50586676470138 40.0 38.0 41.0 35.0 41.0 34-35 38.3674676846521 40.0 38.0 41.0 34.0 41.0 36-37 38.22885470074249 40.0 38.0 41.0 34.0 41.0 38-39 38.103584722340756 40.0 38.0 41.0 34.0 41.0 40-41 37.92613259632239 40.0 37.5 41.0 33.5 41.0 42-43 37.69391670550854 40.0 37.0 41.0 33.0 41.0 44-45 37.37872909376976 40.0 37.0 41.0 33.0 41.0 46-47 36.97166731220773 40.0 37.0 41.0 31.5 41.0 48-49 36.61006092157004 40.0 36.5 41.0 31.0 41.0 50-51 36.322579603966034 39.5 36.0 41.0 30.0 41.0 52-53 36.092687113906265 39.0 36.0 41.0 30.0 41.0 54-55 35.87308060947829 39.0 35.0 40.0 29.0 41.0 56-57 35.59948827194128 39.0 35.0 40.0 28.0 41.0 58-59 35.350245415017255 38.5 35.0 40.0 28.0 41.0 60-61 35.02440254627654 38.0 34.5 40.0 27.5 41.0 62-63 34.66346029703642 38.0 34.0 40.0 26.5 41.0 64-65 34.259133858956936 37.0 34.0 40.0 26.0 41.0 66-67 33.834653410098014 36.5 33.5 39.0 25.5 41.0 68-69 33.38785802910843 36.0 33.0 39.0 24.5 40.0 70-71 32.9814231882726 35.5 32.5 39.0 23.5 40.0 72-73 32.488556964075094 35.0 32.0 38.0 22.5 40.0 74-75 32.001485291511024 35.0 31.5 37.0 21.5 39.0 76-77 30.790462294931302 34.0 30.5 36.0 20.0 38.5 78-79 31.348072458154718 34.5 31.0 36.0 20.0 39.0 80-81 31.14695961101227 34.5 31.0 36.0 20.0 37.5 82-83 30.77711436580514 34.0 31.0 35.5 18.0 37.0 84-85 30.384903858147275 34.0 31.0 35.0 17.0 37.0 86-87 30.009351592298145 34.0 30.0 35.0 9.5 36.0 88-89 29.583872743068092 34.0 30.0 35.0 7.0 36.0 90-91 29.056471166197284 34.0 29.0 35.0 2.0 35.5 92-93 28.611893054634656 34.0 29.0 35.0 2.0 35.0 94-95 28.143892743899634 33.5 29.0 35.0 2.0 35.0 96-97 27.562202257861927 33.0 27.0 35.0 2.0 35.0 98-99 26.682604419002104 33.0 25.5 34.5 2.0 35.0 100-101 23.90496049069874 29.5 13.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31274999861091857 1101 2 0.2440674873222619 1101 3 0.24421029057155863 1101 4 0.12220763040684801 1101 5 0.10712920098155365 1101 6 0.08763406547355146 1101 7 0.15852348405604033 1101 8 0.15471015107572583 1101 9 0.15437395689507838 1101 10-11 0.2064120394710116 1101 12-13 0.16165059708051643 1101 14-15 0.1741969195123616 1101 16-17 0.23584278153342098 1101 18-19 0.33233847950228323 1101 20-21 0.3049095257470711 1101 22-23 0.3196014564760645 1101 24-25 0.21802392828543304 1101 26-27 0.07684850099186491 1101 28-29 -0.08727183358303847 1101 30-31 0.044226488009421416 1101 32-33 0.188974462030707 1101 34-35 0.07296116404486952 1101 36-37 0.01694599579558087 1101 38-39 0.22257084676837735 1101 40-41 0.13547005941389756 1101 42-43 0.09328810772849039 1101 44-45 -0.05070579996131386 1101 46-47 -0.13937263912577436 1101 48-49 -0.3512597490397198 1101 50-51 -0.2822273862381053 1101 52-53 -0.3913328995007177 1101 54-55 -0.40738137628945026 1101 56-57 -0.5445515940683379 1101 58-59 -0.5138192392027321 1101 60-61 -0.34437318821932905 1101 62-63 -0.610201162164465 1101 64-65 -0.7223880491378694 1101 66-67 -0.5565275841118478 1101 68-69 -0.3999279960802511 1101 70-71 -0.49665831234401736 1101 72-73 -0.5415763258098423 1101 74-75 -0.4833798899876882 1101 76-77 -0.507736451086604 1101 78-79 -0.635552234886724 1101 80-81 -0.5726018475922778 1101 82-83 -0.4322004702787723 1101 84-85 -0.3826177975316263 1101 86-87 -0.5056994888815076 1101 88-89 -0.6628870516813059 1101 90-91 -0.6538952376815139 1101 92-93 -0.7023319263194203 1101 94-95 -0.7563123112300723 1101 96-97 -0.6067225377341963 1101 98-99 -1.0625648669058556 1101 100-101 -0.6811403635463762 1102 1 0.32422869535277243 1102 2 0.23656301184786344 1102 3 0.1579911713449249 1102 4 0.11051053051426152 1102 5 -0.011048385842649111 1102 6 0.06420048151365165 1102 7 0.17725961008540025 1102 8 0.1015952208931239 1102 9 0.23404814235873772 1102 10-11 0.1280035181677519 1102 12-13 0.28572506897453565 1102 14-15 0.21744790411673165 1102 16-17 0.10628853878369426 1102 18-19 0.1684054325993074 1102 20-21 0.19521206781653433 1102 22-23 0.2453443852265096 1102 24-25 0.2006519268532827 1102 26-27 0.11575648523820092 1102 28-29 0.14293403823936757 1102 30-31 0.09450396742224854 1102 32-33 0.20214846847539292 1102 34-35 0.06788597607494751 1102 36-37 0.08491413041784313 1102 38-39 0.20761381132261647 1102 40-41 0.19635978372467378 1102 42-43 0.20642452018821444 1102 44-45 0.24839552477911298 1102 46-47 -0.014138124267198293 1102 48-49 0.024726645518100554 1102 50-51 -0.02889226271501144 1102 52-53 0.13421489856587243 1102 54-55 0.04245213606284892 1102 56-57 -0.012500036603249498 1102 58-59 -0.008264280377098032 1102 60-61 -0.003078881022766211 1102 62-63 -0.1935989423291602 1102 64-65 -0.0040153316298159325 1102 66-67 -0.007721640681843667 1102 68-69 0.007757288559922415 1102 70-71 -0.21988602448151084 1102 72-73 -0.12363683422373839 1102 74-75 -0.2451736601273211 1102 76-77 -0.27522839523268416 1102 78-79 0.05973598566465199 1102 80-81 0.19752525588068792 1102 82-83 0.1982793005769352 1102 84-85 0.24255227049558314 1102 86-87 -0.15854231022057164 1102 88-89 -0.20741802196415549 1102 90-91 0.06730060907824154 1102 92-93 -0.04600897608669996 1102 94-95 -0.21964385437364342 1102 96-97 -0.14590084063430453 1102 98-99 -0.21769734094810644 1102 100-101 -0.4182313051861932 1103 1 0.3051846167547865 1103 2 0.19025544793009885 1103 3 0.16748362260051408 1103 4 0.03958192115229764 1103 5 0.03292323998240221 1103 6 0.1514017560755221 1103 7 0.10891805785112041 1103 8 0.06863110587909205 1103 9 0.08774968245433001 1103 10-11 0.23665726219324057 1103 12-13 0.19369259799357508 1103 14-15 0.21699839345744465 1103 16-17 0.05758510201864908 1103 18-19 0.10492049215473287 1103 20-21 0.026506734382046204 1103 22-23 0.04403568330707941 1103 24-25 0.05489212107223551 1103 26-27 -0.009051192479731185 1103 28-29 0.1320410862630368 1103 30-31 -0.06524491354277728 1103 32-33 -0.07758296313071611 1103 34-35 -0.06085804941628936 1103 36-37 -0.1349094793234542 1103 38-39 -0.1399413817583266 1103 40-41 -0.1263247622126613 1103 42-43 -0.06053208401481669 1103 44-45 -0.13212985265811028 1103 46-47 -0.31607158910294686 1103 48-49 -0.34943199191457097 1103 50-51 -0.42874470183192415 1103 52-53 -0.32414448236121984 1103 54-55 -0.47262790338787397 1103 56-57 -0.4401982385606047 1103 58-59 -0.47542003487220086 1103 60-61 -0.46198715808824176 1103 62-63 -0.4822010969456585 1103 64-65 -0.3433526353245284 1103 66-67 -0.32504809542730584 1103 68-69 -0.33147759498457674 1103 70-71 -0.43922825951678846 1103 72-73 -0.6050407489494773 1103 74-75 -0.8028623787374407 1103 76-77 -0.5893692039725345 1103 78-79 -0.4872316192211663 1103 80-81 -0.4922767318288912 1103 82-83 -0.4541074682569928 1103 84-85 -0.5118309325997821 1103 86-87 -0.5678318178451818 1103 88-89 -0.4628462899256185 1103 90-91 -0.4995190555905964 1103 92-93 -0.5268313719595419 1103 94-95 -0.6150353269478046 1103 96-97 -0.47304028377215346 1103 98-99 -0.44227855633388913 1103 100-101 -0.5154894798315155 1104 1 0.26563814069630354 1104 2 0.22302931000461257 1104 3 0.16524395093255606 1104 4 0.12763068856718007 1104 5 0.13413247125816952 1104 6 0.07329272809302267 1104 7 0.05733191089037604 1104 8 0.10817375343899727 1104 9 0.04082760388588014 1104 10-11 0.16735617375109513 1104 12-13 0.1870214392219154 1104 14-15 0.25439277475293665 1104 16-17 0.24535955751044014 1104 18-19 0.2786792929158395 1104 20-21 0.3024816615448529 1104 22-23 0.2689934741102604 1104 24-25 0.18715143293790248 1104 26-27 0.28529430654591437 1104 28-29 0.18983035804432546 1104 30-31 0.25402602547305975 1104 32-33 0.2075865819288012 1104 34-35 0.19916448129784214 1104 36-37 0.21599969337629688 1104 38-39 0.1266537238890919 1104 40-41 0.09511511695992425 1104 42-43 0.22519498615007905 1104 44-45 0.111450233789391 1104 46-47 0.011967090218185206 1104 48-49 -0.006546395109062075 1104 50-51 0.021514008959464093 1104 52-53 0.08275648203757413 1104 54-55 0.04276361801917972 1104 56-57 0.18567226630349865 1104 58-59 0.13881279144030145 1104 60-61 -0.051599867172114955 1104 62-63 0.04211513320836957 1104 64-65 -0.04772411071301974 1104 66-67 -0.18394686609642008 1104 68-69 -0.15706794946526514 1104 70-71 -0.3565030193742089 1104 72-73 -0.1947467776861842 1104 74-75 -0.1161919665230009 1104 76-77 -0.042509338505976046 1104 78-79 0.12911070980765516 1104 80-81 0.02755489080366047 1104 82-83 -0.028905802094410404 1104 84-85 -0.12261093175751014 1104 86-87 0.08192133970901594 1104 88-89 -0.20276681029514165 1104 90-91 -0.025944309898619622 1104 92-93 0.082083669828517 1104 94-95 -0.08962893182378195 1104 96-97 0.04528434610774568 1104 98-99 0.10218706183463411 1104 100-101 0.21970475904382525 1105 1 0.16619145853356088 1105 2 0.1084733253033292 1105 3 0.09597322409293696 1105 4 0.04678933436223076 1105 5 -0.004372604689748982 1105 6 0.017880662477629983 1105 7 0.057146259020250056 1105 8 -0.01267557877875447 1105 9 0.08678522257450538 1105 10-11 0.1554926429284933 1105 12-13 0.12206899996959208 1105 14-15 0.05861472407247703 1105 16-17 0.060820344197694 1105 18-19 0.07320594963925942 1105 20-21 -0.14201281970242263 1105 22-23 -0.07030601622517452 1105 24-25 0.11426300343982376 1105 26-27 -0.06677502188352946 1105 28-29 -0.1384604781542791 1105 30-31 -0.05085385902136608 1105 32-33 0.09916902578479636 1105 34-35 0.16085622142034595 1105 36-37 0.014900458688501317 1105 38-39 0.016607323465606783 1105 40-41 0.2506211374744609 1105 42-43 0.2658985314736029 1105 44-45 0.17973311706328587 1105 46-47 0.05750322898543203 1105 48-49 0.043644838861212065 1105 50-51 0.21696967767625353 1105 52-53 0.2474083659650148 1105 54-55 0.07941104630173612 1105 56-57 0.10221422425335192 1105 58-59 0.2643818206942683 1105 60-61 0.09617588543924427 1105 62-63 -0.03563975865569091 1105 64-65 -0.06121585861284018 1105 66-67 0.16053707815282792 1105 68-69 0.24916970249017112 1105 70-71 -0.01656503713407176 1105 72-73 0.2005487035723874 1105 74-75 0.08888435772049874 1105 76-77 0.008336694627868013 1105 78-79 0.026334210825609716 1105 80-81 -0.16491278632766537 1105 82-83 0.05429759583657656 1105 84-85 0.271421991343729 1105 86-87 -0.01137186104211807 1105 88-89 0.2535504336275807 1105 90-91 0.39386850785772864 1105 92-93 0.29322441276214306 1105 94-95 -0.26208885673512583 1105 96-97 0.0611488001720808 1105 98-99 -0.1106283111437385 1105 100-101 -0.4520909586699986 1106 1 0.19718935787864922 1106 2 0.12206138511021436 1106 3 0.16800891757385017 1106 4 0.11778352666924263 1106 5 -0.01213090580715459 1106 6 0.05426259331488836 1106 7 0.10392775560447376 1106 8 -0.015160176918172397 1106 9 0.002981889770744317 1106 10-11 -0.06861656464798926 1106 12-13 -0.1184504708066001 1106 14-15 0.038270146056980536 1106 16-17 0.058773750259589974 1106 18-19 0.00544107294240348 1106 20-21 0.1932104410788824 1106 22-23 0.16428109034333715 1106 24-25 -0.035246475986113524 1106 26-27 0.04866390907729823 1106 28-29 0.1509146652727935 1106 30-31 0.02182946283780751 1106 32-33 0.11283983197731118 1106 34-35 -0.0035167154364401654 1106 36-37 0.176900229205188 1106 38-39 0.26205406568523415 1106 40-41 -0.15105740054033845 1106 42-43 -0.12461807076120834 1106 44-45 0.08238916571374233 1106 46-47 0.07537755156835146 1106 48-49 0.040225758206659634 1106 50-51 0.2782417213133854 1106 52-53 0.2091343011095006 1106 54-55 0.12333944750916714 1106 56-57 0.31174131211015066 1106 58-59 0.42472004378735306 1106 60-61 0.5026901222307103 1106 62-63 0.28863560366315255 1106 64-65 0.06789608511079592 1106 66-67 0.2785124616547421 1106 68-69 0.6265594181073908 1106 70-71 0.22246830855605282 1106 72-73 0.46790117228321293 1106 74-75 0.4566468071900367 1106 76-77 0.3815846778226266 1106 78-79 0.24761900843898133 1106 80-81 0.3207047885633294 1106 82-83 0.25449861438941923 1106 84-85 0.13749089812055004 1106 86-87 0.30124177732749047 1106 88-89 0.3354539094170903 1106 90-91 0.14128020091497717 1106 92-93 -0.00913970663978958 1106 94-95 -0.38787218918583477 1106 96-97 -0.262090577398574 1106 98-99 -0.0805759271652029 1106 100-101 0.05797100182356729 1107 1 0.19844375386458069 1107 2 0.11009422340512742 1107 3 0.05593647620565889 1107 4 0.011400667094392247 1107 5 -0.025402863336893233 1107 6 -0.03370192519864901 1107 7 -0.006422691274096337 1107 8 -0.036972107119993325 1107 9 -0.055031124587607394 1107 10-11 -0.02791169490357248 1107 12-13 -0.026060566454297884 1107 14-15 -0.050993568299126935 1107 16-17 -0.005451044221075563 1107 18-19 0.3297794350596419 1107 20-21 0.14926878169219293 1107 22-23 0.17214626517477427 1107 24-25 -0.009135159542331905 1107 26-27 0.07508462453100861 1107 28-29 0.1564488475634036 1107 30-31 0.3126631321700657 1107 32-33 0.20612853345346593 1107 34-35 0.04384093301908365 1107 36-37 0.20255094712288724 1107 38-39 0.13627986816266713 1107 40-41 0.182422932322595 1107 42-43 0.485043578321509 1107 44-45 0.2899203016141101 1107 46-47 0.060331799714752776 1107 48-49 -0.022795040126794675 1107 50-51 -0.11722901318027112 1107 52-53 -0.02644434503883275 1107 54-55 -0.1912563457842893 1107 56-57 -0.1795227158449606 1107 58-59 -0.04004486896492665 1107 60-61 -0.2621414303083256 1107 62-63 -0.2033368220250935 1107 64-65 -0.040221368913350375 1107 66-67 0.03656943587242267 1107 68-69 0.092337927616164 1107 70-71 -0.1978439464447277 1107 72-73 0.0077898446394613075 1107 74-75 -0.045752785131266904 1107 76-77 -0.002280093810103523 1107 78-79 0.058693812999607076 1107 80-81 -0.021370116796976646 1107 82-83 0.08347376166894804 1107 84-85 0.28162743688362113 1107 86-87 0.3858743065033998 1107 88-89 0.37234099137842946 1107 90-91 0.4536836012240961 1107 92-93 0.3018804980955565 1107 94-95 0.39310131169896323 1107 96-97 0.6102200312074793 1107 98-99 0.8431789168192978 1107 100-101 0.8598660357594596 1108 1 0.06968981651009187 1108 2 0.003269289295552369 1108 3 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84-85 0.5177273701212322 2315 86-87 0.4316979989208356 2315 88-89 0.29701380994020354 2315 90-91 0.5761914435185282 2315 92-93 0.6583630804282166 2315 94-95 0.7268263786899922 2315 96-97 0.7736106917893153 2315 98-99 0.9901030454786515 2315 100-101 1.4129684291454083 2316 1 -0.4699923985775314 2316 2 -0.23394175225487146 2316 3 -0.13421416313135381 2316 4 -0.15842751966542323 2316 5 -0.07369584224039727 2316 6 -0.10468943309516732 2316 7 -0.1255184058059342 2316 8 -0.18955904372063515 2316 9 -0.22855975055610145 2316 10-11 -0.10527134912972969 2316 12-13 -0.11660522016858721 2316 14-15 -0.2427347472960193 2316 16-17 -0.31009931522705614 2316 18-19 -0.2613008085401205 2316 20-21 -0.23296636349137145 2316 22-23 -0.1568744667730968 2316 24-25 -0.1452898488877068 2316 26-27 -0.17913455033596648 2316 28-29 -0.08289366279257848 2316 30-31 0.11145362289024519 2316 32-33 0.011810530392239116 2316 34-35 0.010119994076333683 2316 36-37 -0.09032308563173785 2316 38-39 -0.17320119642522513 2316 40-41 -0.03252089940479408 2316 42-43 -0.10134245089748362 2316 44-45 -0.19232782756667177 2316 46-47 -0.12806974538091254 2316 48-49 -0.00992964672416008 2316 50-51 0.07212385940427168 2316 52-53 0.1191062638357252 2316 54-55 0.13173306976105437 2316 56-57 0.13090967623180205 2316 58-59 0.3265720257123803 2316 60-61 0.2371993821403393 2316 62-63 0.3310807583071522 2316 64-65 0.2909080141220528 2316 66-67 0.21555883144238663 2316 68-69 0.4416012106198579 2316 70-71 0.4002913518001847 2316 72-73 0.29394715575351427 2316 74-75 0.43518570817573377 2316 76-77 0.48561232877905525 2316 78-79 0.31961597643317674 2316 80-81 -0.009005356364209405 2316 82-83 -0.10675053688309077 2316 84-85 -0.15962372106420375 2316 86-87 -0.2930489206191744 2316 88-89 -0.1542150283272825 2316 90-91 0.3490750312865458 2316 92-93 0.4023204558476259 2316 94-95 0.386541896178592 2316 96-97 0.29914218465335196 2316 98-99 -0.08061251024684069 2316 100-101 0.42191705787470113 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 2.0 4 8.0 5 15.0 6 22.0 7 76.0 8 164.0 9 327.0 10 615.0 11 1019.0 12 1649.0 13 2427.0 14 3248.0 15 4677.0 16 6908.0 17 10294.0 18 9583.0 19 5329.0 20 4211.0 21 4115.0 22 4288.0 23 4644.0 24 5098.0 25 5941.0 26 7244.0 27 8839.0 28 11182.0 29 13800.0 30 17993.0 31 22951.0 32 30371.0 33 40826.0 34 58135.0 35 86617.0 36 140130.0 37 218745.0 38 168714.0 39 13749.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.53170291837565 19.99877434350774 15.69901816160281 21.770504576513794 2 15.558961969972493 20.678321418177124 40.674010516848625 23.08870609500176 3 19.517879299139352 24.642928261787688 30.295570275350613 25.54362216372234 4 13.124725097980003 17.451819659898334 37.03687899496916 32.38657624715251 5 13.507008632323439 37.71527451707913 34.14435801540838 14.633358835189059 6 31.540370387390286 36.79726290589762 17.198964508371244 14.46340219834085 7 28.006963090369187 33.7121236987971 20.40500589740055 17.875907313433164 8 25.86245380004858 36.16474207899235 19.204737177256824 18.768066943702255 9 26.187850260732944 17.45860331173506 20.571205367400395 35.7823410601316 10-11 24.858965690039632 27.463067392298584 26.728190012276222 20.949776905385566 12-13 25.553045057662427 24.772304527649517 28.008184344705338 21.66646606998271 14-15 22.652911171361612 26.14517890240513 26.519811545775422 24.68209838045783 16-17 21.393832566343022 29.591383449202485 27.26940507373392 21.74537891072058 18-19 21.194918388291857 27.744315409174565 30.28265945411297 20.778106748420612 20-21 22.034723544304903 26.551267995824773 30.726441580722174 20.687566879148147 22-23 21.902816931458915 26.416329897899843 30.604662918490018 21.076190252151225 24-25 21.639294936321065 26.76528513282857 30.616985426058037 20.978434504792332 26-27 21.34819843033504 26.777635173799965 30.83107118199732 21.043095213867673 28-29 21.086290994951607 27.19567943244408 30.82687968978745 20.89114988281686 30-31 21.451055951998004 26.77496438582786 30.78235200150553 20.991627660668605 32-33 21.46812449532913 26.742796746473047 30.658386234876282 21.13069252332154 34-35 21.771255260065516 26.845973902634903 30.515820132565686 20.866950704733895 36-37 21.753037872471722 26.61571268827369 30.589127337898077 21.04212210135651 38-39 21.401327407485212 27.03427494797377 30.54098529260516 21.023412351935857 40-41 21.637835767821 27.149023469555228 30.211499534985503 21.001641227638274 42-43 21.576520113431624 27.029740511208296 30.03640076182806 21.357338613532022 44-45 21.763596298314372 27.12951960803622 29.886745838448427 21.220138255200983 46-47 21.88964092599036 27.626039156989023 29.297498145437118 21.1868217715835 48-49 22.01557613992704 27.690210315089686 29.06532219063812 21.228891354345148 50-51 22.046266693801275 27.899628212113853 28.8239007748681 21.23020431921677 52-53 22.02547808333388 28.159048187999066 28.437123206435277 21.378350522231777 54-55 21.968157302030775 28.046476793606733 28.651042740859435 21.334323163503058 56-57 22.122801604443072 28.290180554552595 28.531000194756455 21.056017646247874 58-59 22.0474702449336 27.904497123512794 28.677176950464023 21.370855681089584 60-61 21.96431580306402 28.00187535149164 29.050332508353737 20.9834763370906 62-63 21.810097137517754 27.830642849483894 29.189780319094226 21.169479693904123 64-65 21.802109934548703 28.00072650722897 28.862304997363132 21.334858560859203 66-67 21.72907626356457 28.0765502285653 28.732047940724016 21.462325567146117 68-69 21.61090942511833 27.970692435790546 28.84534586777131 21.573052271319813 70-71 21.861357474380775 27.919869753884736 28.667165593317883 21.551607178416607 72-73 21.876894225361667 27.906521277689883 28.564863747070447 21.651720749878002 74-75 21.71862725146122 28.493963644002708 28.076823762913556 21.71058534162252 76-77 21.768027554756106 28.215615091218233 28.309601493278713 21.706755860746945 78-79 21.993583978327326 28.128795289082042 28.20691669894372 21.670704033646913 80-81 21.869180556740872 28.4543011635057 28.04301491746922 21.63350336228421 82-83 21.721505192810156 28.482826306736868 27.980066874116478 21.8156016263365 84-85 21.80396996811684 28.345981561596652 28.10510721452314 21.74494125576337 86-87 21.58191478420328 28.479028668587976 28.091321083371078 21.847735463837665 88-89 21.68197364879503 28.39860957020095 27.937266538433764 21.98215024257026 90-91 21.80218450253954 28.30371402637095 27.995073570889637 21.89902790019987 92-93 21.732924705017332 28.071824340884422 28.151642537026017 22.04360841707223 94-95 21.506309890345552 28.415295165444515 28.053080981485007 22.025313962724926 96-97 21.45882432748845 28.312829198588123 28.053354515833263 22.17499195809016 98-99 21.57315640092522 28.35249566166929 27.753773198854198 22.320574738551294 100-101 21.336834490962396 28.477346620076975 27.845912586329725 22.3399063026309 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 209.0 1 149.5 2 112.0 3 144.0 4 146.5 5 160.5 6 271.5 7 604.5 8 1050.0 9 1350.5 10 1418.0 11 1434.5 12 1591.5 13 1758.0 14 1900.0 15 2002.0 16 2028.0 17 2024.5 18 2075.0 19 2120.5 20 2202.0 21 2451.0 22 2893.5 23 3458.5 24 4044.0 25 4954.0 26 6078.0 27 7265.0 28 8404.0 29 9814.5 30 11628.5 31 13180.5 32 14728.5 33 16616.5 34 18760.0 35 20618.5 36 23292.5 37 25809.5 38 27274.0 39 28751.0 40 29371.0 41 30391.5 42 31626.5 43 33048.0 44 35164.5 45 36599.0 46 37267.5 47 38234.5 48 38610.5 49 36453.5 50 34291.5 51 32096.0 52 29672.5 53 28522.5 54 27163.5 55 24626.5 56 21237.0 57 18279.5 58 16130.5 59 14108.0 60 11437.0 61 8913.0 62 6927.0 63 5196.0 64 3887.5 65 2844.5 66 2066.5 67 1629.5 68 1167.0 69 703.0 70 483.0 71 343.5 72 252.0 73 166.5 74 115.0 75 90.5 76 68.5 77 38.0 78 18.0 79 11.5 80 10.5 81 9.5 82 4.5 83 4.5 84 5.5 85 4.0 86 1.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.01816268072414389 2 0.0 3 0.0 4 0.0 5 0.033152363008527704 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.002407102264645576 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.111893054634656E-4 24-25 2.1882747860414327E-4 26-27 9.847236537186448E-4 28-29 8.753099144165731E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0013129648716248598 42-43 0.011761976974972702 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 5.4706869651035817E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.564824358124299E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.012637286889389273 92-93 0.0010941373930207165 94-95 0.0 96-97 0.0 98-99 0.0017506198288331461 100-101 0.01849092194205011 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 913962.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.52743072346718 #Duplication Level Percentage of deduplicated Percentage of total 1 81.14901300725784 49.92890276081794 2 11.012362188309895 13.551247032859331 3 3.3041121628065078 6.098805965989282 4 1.4550487326702857 3.581016403945589 5 0.7705136209361686 2.3703861716818984 6 0.4690764621733595 1.731664171822827 7 0.335892258292556 1.4466611356850927 8 0.22413633455185167 1.1032426237400734 9 0.18320717219969318 1.014503993600305 >10 0.9942280216647827 11.292162674513891 >50 0.07133057656731318 2.996464294570727 >100 0.029642262036734614 3.0714102794843248 >500 5.38950199899939E-4 0.1895659305515934 >1k 7.186002665332521E-4 1.0528370195358177 >5k 1.7965006663331302E-4 0.5711295412012146 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5167 0.5653407909738042 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4485 0.49072062076979134 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2831 0.3097502959641648 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1186 0.12976469481225694 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1023 0.11193025530601929 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.0941373930207163E-4 0.0 16-17 0.0 0.0 0.0 1.0941373930207163E-4 0.0 18-19 0.0 0.0 0.0 1.0941373930207163E-4 0.0 20-21 0.0 0.0 0.0 1.0941373930207163E-4 0.0 22-23 0.0 0.0 0.0 2.1882747860414327E-4 0.0 24-25 0.0 0.0 0.0 2.1882747860414327E-4 0.0 26-27 0.0 0.0 0.0 5.470686965103582E-4 0.0 28-29 0.0 0.0 0.0 0.0014770854805779671 0.0 30-31 0.0 0.0 0.0 0.004103015223827686 0.0 32-33 0.0 0.0 0.0 0.007823082360098122 0.0 34-35 0.0 0.0 0.0 0.01203551132322788 0.0 36-37 0.0 0.0 0.0 0.015864992198800387 0.0 38-39 0.0 0.0 0.0 0.024563384473315082 0.0 40-41 0.0 0.0 0.0 0.03938894614874579 0.0 42-43 0.0 5.4706869651035817E-5 0.0 0.05224506051673921 0.0 44-45 0.0 1.0941373930207163E-4 0.0 0.07051715498018518 0.0 46-47 0.0 1.0941373930207163E-4 0.0 0.10542013781754603 0.0 48-49 0.0 1.0941373930207163E-4 0.0 0.12916291924609558 0.0 50-51 0.0 1.0941373930207163E-4 0.0 0.15536750980894173 0.0 52-53 0.0 1.0941373930207163E-4 0.0 0.18310389272201688 0.0 54-55 0.0 1.0941373930207163E-4 0.0 0.22112516712948677 0.0 56-57 0.0 1.0941373930207163E-4 0.0 0.30767143491742543 0.0 58-59 0.0 1.0941373930207163E-4 0.0 0.3886376020009584 0.0 60-61 0.0 1.6412060895310744E-4 0.0 0.45401231123394625 0.0 62-63 0.0 2.1882747860414327E-4 0.0 0.5333919790975993 0.0 64-65 0.0 2.1882747860414327E-4 0.0 0.6137016637453199 0.0 66-67 0.0 2.1882747860414327E-4 0.0 0.7282031419249377 0.0 68-69 0.0 2.1882747860414327E-4 0.0 0.8686356763191467 0.0 70-71 0.0 2.1882747860414327E-4 0.0 0.9672174554303132 0.0 72-73 0.0 2.1882747860414327E-4 0.0 1.0730205413354166 0.0 74-75 0.0 2.1882747860414327E-4 0.0 1.2489031272634967 0.0 76-77 0.0 2.1882747860414327E-4 0.0 1.4634087631652082 0.0 78-79 0.0 2.1882747860414327E-4 0.0 1.6763826067166905 0.0 80-81 0.0 2.1882747860414327E-4 0.0 1.8349231149653926 0.0 82-83 0.0 2.1882747860414327E-4 0.0 2.07349977351356 0.0 84-85 0.0 2.1882747860414327E-4 0.0 2.389760186966198 0.0 86-87 0.0 2.1882747860414327E-4 0.0 2.6744000297605375 0.0 88-89 0.0 2.1882747860414327E-4 0.0 3.038638367897134 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3265 0.0 44.09871 1 GTATCAA 4885 0.0 34.435402 1 CGCCCGA 45 2.413942E-5 31.666273 34-35 GGACCGA 230 0.0 28.911894 6 TATCAAC 6200 0.0 27.0492 2 ATCAACG 6190 0.0 26.939398 3 TCAACGC 6225 0.0 26.78793 4 CAACGCA 6400 0.0 26.055449 5 AACGCAG 6550 0.0 25.453186 6 GAGTACT 4490 0.0 24.067732 12-13 GTACTTT 4630 0.0 23.23612 14-15 ACCGAGT 250 3.8198777E-10 22.800343 8 TACATGG 3590 0.0 22.6294 2 GTACATG 3655 0.0 22.36184 1 ACGCAGA 7565 0.0 21.850954 7 CGCAGAG 7525 0.0 21.777735 8 CGCGGAA 55 0.0028786722 21.590052 82-83 ACATGGG 3595 0.0 21.540714 3 AGGACCG 270 9.986252E-10 21.114893 5 TACACGG 135 3.790537E-4 21.114893 5 >>END_MODULE