FastQCFastQC Report
Thu 26 May 2016
SRR937682_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937682_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873584
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36200.4143848788439349No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28640.3278448323229363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18150.20776479422700048No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN10340.11836297368083666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22300.044.1358451
GTATCAA37250.038.420781
GGACCGA905.692418E-736.94736
ATCAACG45950.031.0250453
TATCAAC46300.030.893152
TCAACGC46700.030.5285384
CAACGCA46900.030.4996785
AACGCAG48600.029.4209986
TATGACG700.00837089727.1558594
CGCAGAG54700.025.3599038
ACTGCGC1501.0583753E-625.3367468
ACGCAGA56250.025.2522877
GTACACG950.001219219825.0249021
GTACATG31950.023.6620431
TACATGG31250.023.5699332
ACATGGG31050.023.109583
GAGTACT32400.023.01875512-13
GCAGAGT58900.022.9049959
GTGCGGA1050.002183129822.6415771
AGGACCG1754.030615E-621.7246885
Produced by FastQC (version 0.11.5)