##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937682_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 873584 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.821904934156304 33.0 31.0 34.0 30.0 34.0 2 31.971687897214235 33.0 31.0 34.0 30.0 34.0 3 32.01164742028242 34.0 31.0 34.0 30.0 34.0 4 35.58015714573527 37.0 35.0 37.0 33.0 37.0 5 35.55546690415575 37.0 35.0 37.0 33.0 37.0 6 35.63355670433524 37.0 35.0 37.0 33.0 37.0 7 35.57457210754776 37.0 35.0 37.0 33.0 37.0 8 35.55956725397901 37.0 35.0 37.0 33.0 37.0 9 37.19958012051503 39.0 37.0 39.0 34.0 39.0 10-11 37.08317173849338 39.0 37.0 39.0 34.0 39.0 12-13 36.961631050935 39.0 37.0 39.0 33.0 39.0 14-15 38.14107859118299 40.0 38.0 41.0 33.0 41.0 16-17 38.08653947416619 40.0 38.0 41.0 33.0 41.0 18-19 38.02404290829502 40.0 38.0 41.0 33.0 41.0 20-21 37.78383360958992 40.0 38.0 41.0 32.5 41.0 22-23 37.69866034634334 40.0 37.5 41.0 32.5 41.0 24-25 37.56667303888349 40.0 37.0 41.0 32.0 41.0 26-27 37.66366943533764 40.0 37.0 41.0 33.0 41.0 28-29 37.600530687375226 40.0 37.0 41.0 32.5 41.0 30-31 37.3986989230572 40.0 37.0 41.0 32.0 41.0 32-33 37.167620400556785 39.5 36.5 41.0 31.5 41.0 34-35 36.91704060513929 39.0 36.0 41.0 31.0 41.0 36-37 36.973412402241806 39.0 36.0 41.0 31.0 41.0 38-39 36.9508616229235 40.0 36.0 41.0 31.0 41.0 40-41 36.77110787285481 39.5 36.0 41.0 30.5 41.0 42-43 36.544145726112205 39.0 36.0 41.0 30.0 41.0 44-45 36.31133411326215 39.0 36.0 41.0 30.0 41.0 46-47 35.96794870327295 39.0 35.0 41.0 28.5 41.0 48-49 35.62872374036155 39.0 35.0 40.5 27.5 41.0 50-51 34.75679671330977 38.0 34.0 39.5 26.0 40.5 52-53 34.86851579241378 38.0 34.5 40.0 26.0 40.5 54-55 35.18515906884741 39.0 35.0 40.0 26.0 41.0 56-57 34.93441672466528 38.5 34.5 40.0 25.5 41.0 58-59 34.710186427407095 38.0 34.0 40.0 25.0 41.0 60-61 34.40618360684262 38.0 34.0 40.0 24.5 41.0 62-63 34.04342684847708 38.0 34.0 40.0 23.0 41.0 64-65 33.691228891554786 37.0 33.0 40.0 22.0 41.0 66-67 33.271720864851005 36.5 33.0 39.5 21.5 41.0 68-69 32.78803354914925 36.0 32.0 39.0 19.5 40.5 70-71 32.34842785582154 35.5 32.0 39.0 18.0 40.0 72-73 31.86657436491511 35.0 31.0 38.0 15.0 40.0 74-75 31.373647525595707 35.0 31.0 37.5 10.0 39.5 76-77 30.89267088225059 35.0 30.5 37.0 7.5 39.0 78-79 30.353214459056026 34.0 30.0 36.0 4.5 39.0 80-81 29.799577945566767 34.0 29.5 36.0 2.0 37.5 82-83 29.269967169728382 34.0 29.0 35.0 2.0 37.0 84-85 28.79067496657448 34.0 29.0 35.0 2.0 37.0 86-87 28.267655428670857 34.0 27.5 35.0 2.0 36.0 88-89 27.778112350958807 33.5 26.5 35.0 2.0 36.0 90-91 27.25579509240096 33.0 26.0 35.0 2.0 35.0 92-93 26.68857144819502 33.0 25.0 35.0 2.0 35.0 94-95 26.04666465960915 32.0 24.5 34.5 2.0 35.0 96-97 25.26683467188044 32.0 20.5 34.0 2.0 35.0 98-99 24.330395817688967 31.5 9.5 34.0 2.0 35.0 100-101 21.81497715159618 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.20033664934894446 1101 2 0.20522017416763205 1101 3 0.21170823883833378 1101 4 0.1320916338700613 1101 5 0.18524635453091065 1101 6 0.21492845943506467 1101 7 0.12463546004990178 1101 8 0.17958036655249288 1101 9 0.3332798200089897 1101 10-11 0.21512498154754667 1101 12-13 0.24088417725496214 1101 14-15 0.24701752954913303 1101 16-17 0.23943532994127992 1101 18-19 0.29793614375893895 1101 20-21 0.4743447049160707 1101 22-23 0.4842584965591499 1101 24-25 0.37462946575789857 1101 26-27 0.4006981840291033 1101 28-29 0.34645848062839235 1101 30-31 0.2708999437863753 1101 32-33 0.38753893923709626 1101 34-35 0.28989529324251606 1101 36-37 0.2750399951510829 1101 38-39 0.1950802093782258 1101 40-41 0.31858722854941135 1101 42-43 0.3784884368073236 1101 44-45 0.4130830458289694 1101 46-47 0.32630383859968504 1101 48-49 0.4513586061264405 1101 50-51 0.4056293749359199 1101 52-53 0.09544741312788574 1101 54-55 0.20540127021207866 1101 56-57 0.3317256181427055 1101 58-59 0.29026960285288794 1101 60-61 0.4592041747814548 1101 62-63 0.521862182991967 1101 64-65 0.6195673645925197 1101 66-67 0.678427755733928 1101 68-69 0.5905126738140538 1101 70-71 0.553308784681029 1101 72-73 0.5418607629054684 1101 74-75 0.43742426881852126 1101 76-77 0.37871806340449155 1101 78-79 0.37351672691958626 1101 80-81 0.559709092225912 1101 82-83 0.7379138109560905 1101 84-85 0.934365159959345 1101 86-87 0.7651970663674277 1101 88-89 0.8428850703623176 1101 90-91 0.9275693230864697 1101 92-93 0.9902268094519471 1101 94-95 1.1980398479542664 1101 96-97 1.1714395502537762 1101 98-99 1.3272883356413558 1101 100-101 1.4037734891597609 1102 1 0.1544073001141868 1102 2 0.25617463873416924 1102 3 0.28504213905522136 1102 4 0.1859515473827571 1102 5 0.24088785327539597 1102 6 0.2008217671698489 1102 7 0.2348284019399003 1102 8 0.26949458614337374 1102 9 0.2895259717746441 1102 10-11 0.17858071391840724 1102 12-13 0.27893726009406095 1102 14-15 0.45812590299972555 1102 16-17 0.43012283333489876 1102 18-19 0.3894273070347225 1102 20-21 0.5113720187998396 1102 22-23 0.4921731812677379 1102 24-25 0.43369839026027535 1102 26-27 0.3511155093563971 1102 28-29 0.5216270345733349 1102 30-31 0.3548129667748654 1102 32-33 0.40790551141179066 1102 34-35 0.44607956591214304 1102 36-37 0.4255847156040744 1102 38-39 0.6132973537808866 1102 40-41 0.5918249293107039 1102 42-43 0.6503438466692515 1102 44-45 0.6143092047045684 1102 46-47 0.5992306071483071 1102 48-49 0.7374108632279359 1102 50-51 0.6328183735702808 1102 52-53 0.5972653676959254 1102 54-55 0.7515165640814416 1102 56-57 0.8736228373739863 1102 58-59 0.6660383532946952 1102 60-61 0.747890903661883 1102 62-63 0.8740062322001947 1102 64-65 0.8362041332743217 1102 66-67 0.731685270099824 1102 68-69 0.7452040108430538 1102 70-71 1.0392781606754298 1102 72-73 0.9158074608258566 1102 74-75 0.7503649908983938 1102 76-77 0.7146554753625232 1102 78-79 0.7258026677635812 1102 80-81 0.7082360828836016 1102 82-83 0.6519592388182822 1102 84-85 0.7571110038662532 1102 86-87 1.0472489526041429 1102 88-89 1.0135533031301662 1102 90-91 1.083582762324582 1102 92-93 1.02361155502539 1102 94-95 1.0204081679060728 1102 96-97 1.1399675244202747 1102 98-99 1.1658325581930065 1102 100-101 1.0688756767497267 1103 1 -0.024422743186168105 1103 2 0.06905301326178304 1103 3 0.01415930119664921 1103 4 -0.07765630983330851 1103 5 -0.0700227811704508 1103 6 -0.10486249274601533 1103 7 -4.3605960193815463E-4 1103 8 -0.030990550798655647 1103 9 0.013541761089307158 1103 10-11 0.1444996808237562 1103 12-13 0.06222666510372932 1103 14-15 0.16143139827602937 1103 16-17 0.2941781492544919 1103 18-19 0.05420769207550791 1103 20-21 -0.028205830898350825 1103 22-23 -0.005892550670544949 1103 24-25 0.028846309929797087 1103 26-27 -0.11622083468146371 1103 28-29 -0.006133118556711281 1103 30-31 0.07651237381654852 1103 32-33 -0.06531183692884923 1103 34-35 -0.029244446204145902 1103 36-37 0.026572762242146553 1103 38-39 0.08722857440771037 1103 40-41 0.13437023650387658 1103 42-43 0.2770929078235227 1103 44-45 0.06004128517722762 1103 46-47 0.13465733393427115 1103 48-49 0.19326423089822953 1103 50-51 0.18430633815746944 1103 52-53 0.09596440210219725 1103 54-55 0.2186899912075262 1103 56-57 0.3471843847173446 1103 58-59 0.2113576911766728 1103 60-61 0.2750710725859875 1103 62-63 0.46459840259113605 1103 64-65 0.4918887190240113 1103 66-67 0.327643627352316 1103 68-69 0.46721592214279895 1103 70-71 0.5656644799203008 1103 72-73 0.20255248164071205 1103 74-75 0.2740267469531297 1103 76-77 0.2259367309720517 1103 78-79 0.3046920720623376 1103 80-81 0.554926233282572 1103 82-83 0.2443855574233318 1103 84-85 0.6002483807380905 1103 86-87 0.5246612633169079 1103 88-89 0.7607995630021911 1103 90-91 0.740974124884616 1103 92-93 0.9947417896198196 1103 94-95 0.8194836292423382 1103 96-97 0.8929369072969386 1103 98-99 0.8642135195449683 1103 100-101 0.8615046639930313 1104 1 0.2065233409493814 1104 2 0.26186658214644254 1104 3 0.3011581375448955 1104 4 0.20937073625435687 1104 5 0.27592735811514046 1104 6 0.24237118968284932 1104 7 0.25874797368555846 1104 8 0.27442219295112125 1104 9 0.46265491740652465 1104 10-11 0.34601948053460774 1104 12-13 0.26948009435017894 1104 14-15 0.3808573300789817 1104 16-17 0.4989491214326378 1104 18-19 0.5018398370735682 1104 20-21 0.5259498163388585 1104 22-23 0.44791752725885914 1104 24-25 0.5327251490990221 1104 26-27 0.43993770405403865 1104 28-29 0.46702884060969296 1104 30-31 0.46947404383312374 1104 32-33 0.6194619562232333 1104 34-35 0.6299607443879154 1104 36-37 0.5381458078361376 1104 38-39 0.6908570521396484 1104 40-41 0.7730986824998496 1104 42-43 0.8366542463756517 1104 44-45 0.9943125923460272 1104 46-47 1.0265999271145745 1104 48-49 0.8690849580042652 1104 50-51 0.8355810657571752 1104 52-53 0.997551199944148 1104 54-55 0.9878073789856501 1104 56-57 1.1529426977749253 1104 58-59 1.2066713482167657 1104 60-61 1.0436595277501297 1104 62-63 1.082864208860748 1104 64-65 1.2158740499595169 1104 66-67 1.0047796335584565 1104 68-69 1.0904113803747464 1104 70-71 1.187569342574129 1104 72-73 1.0951333201752185 1104 74-75 1.2742171626941676 1104 76-77 1.5144201048568817 1104 78-79 1.1820643348360598 1104 80-81 1.2119063806784638 1104 82-83 1.3726340852275563 1104 84-85 1.1847859173225963 1104 86-87 1.3740953054199423 1104 88-89 1.1172578302189464 1104 90-91 1.4479137207801003 1104 92-93 1.7604387464941809 1104 94-95 1.7914713580056905 1104 96-97 1.8461863166555226 1104 98-99 2.076252025839267 1104 100-101 1.9092355430790384 1105 1 0.29128485393378867 1105 2 0.221114941954891 1105 3 0.38322045427590723 1105 4 0.44723859271913824 1105 5 0.2502570418904284 1105 6 0.25618044226179393 1105 7 0.23898867575082505 1105 8 0.21208647580241546 1105 9 0.27048775499064703 1105 10-11 0.2637662476168572 1105 12-13 0.3762032975229701 1105 14-15 0.3530421074137493 1105 16-17 0.4460948717016109 1105 18-19 0.3919512224289292 1105 20-21 0.5136383592580813 1105 22-23 0.6285347877697944 1105 24-25 0.41686750295848185 1105 26-27 0.5128059824330435 1105 28-29 0.35102510666880704 1105 30-31 0.5259894542501868 1105 32-33 0.4340174695873671 1105 34-35 0.6758850529759783 1105 36-37 0.6555429135045543 1105 38-39 0.6539216261117602 1105 40-41 0.5426512424644088 1105 42-43 0.562205371831844 1105 44-45 0.6747679432731815 1105 46-47 0.5615513015001596 1105 48-49 0.604050044602161 1105 50-51 0.40653121659151026 1105 52-53 0.45195123791643255 1105 54-55 0.49642128527850105 1105 56-57 0.48949049010875 1105 58-59 0.791255657226742 1105 60-61 0.7854258716524143 1105 62-63 0.845723627041032 1105 64-65 1.0053335778374972 1105 66-67 0.9512455745326562 1105 68-69 0.8343827534945945 1105 70-71 0.9441759919423092 1105 72-73 0.7868110620902904 1105 74-75 0.6567815573527298 1105 76-77 0.8704851218389287 1105 78-79 0.8636630733125266 1105 80-81 0.6869032163217739 1105 82-83 0.8296911280307562 1105 84-85 1.0888204720976127 1105 86-87 0.859999151168104 1105 88-89 0.7731952181479791 1105 90-91 0.8903656752977476 1105 92-93 0.8937335801544961 1105 94-95 1.4524801549172786 1105 96-97 1.1570759227985938 1105 98-99 1.4836545109140502 1105 100-101 1.8023446290018725 1106 1 0.11875279052905086 1106 2 0.19001143360522477 1106 3 0.26785523519030363 1106 4 0.08474446671196034 1106 5 -0.030390873844993394 1106 6 -0.048556574781379425 1106 7 0.017441723923298014 1106 8 0.14728917153531995 1106 9 0.17278462945338902 1106 10-11 0.0098750855405072 1106 12-13 -0.15416667806413642 1106 14-15 0.04067892846614285 1106 16-17 0.13406837283056205 1106 18-19 0.011476947652141689 1106 20-21 0.14951670743399603 1106 22-23 0.022479130971433392 1106 24-25 0.061720166026255185 1106 26-27 -0.035502594169557256 1106 28-29 0.19297315191860065 1106 30-31 0.4546104126565851 1106 32-33 0.50105691989625 1106 34-35 0.06408147929604979 1106 36-37 0.28883240679387967 1106 38-39 0.23327783649812517 1106 40-41 0.23978817240057282 1106 42-43 0.04590146003916118 1106 44-45 0.07522095706130472 1106 46-47 0.24823825493228924 1106 48-49 0.7079681410846419 1106 50-51 0.45370081165849996 1106 52-53 0.5682233441869897 1106 54-55 0.7887355238371114 1106 56-57 0.8014553836891949 1106 58-59 0.5722101667340169 1106 60-61 0.37995417570174794 1106 62-63 0.4518151008688207 1106 64-65 0.5195371790249013 1106 66-67 0.636017033231326 1106 68-69 0.6036222290186686 1106 70-71 0.40113395134586227 1106 72-73 0.5552611302859098 1106 74-75 0.5613277412631454 1106 76-77 0.8327053192050897 1106 78-79 0.9208657346610813 1106 80-81 0.7712924356824544 1106 82-83 0.44858790621732325 1106 84-85 0.33803822817504425 1106 86-87 0.48706651010631674 1106 88-89 1.0025216287658658 1106 90-91 1.2001256725469993 1106 92-93 1.080519388587831 1106 94-95 0.938173400685141 1106 96-97 1.022982695369052 1106 98-99 1.1594731449385094 1106 100-101 1.1042277451407365 1107 1 0.2720087247781464 1107 2 0.36082105867946623 1107 3 0.4330563003856156 1107 4 0.2887264963305185 1107 5 0.4315992788457734 1107 6 0.42433458754595677 1107 7 0.2025241999276659 1107 8 0.3368115578793649 1107 9 0.4399407878532955 1107 10-11 0.4173098998018645 1107 12-13 0.46804580541192564 1107 14-15 0.3498944964106556 1107 16-17 0.34131926847590677 1107 18-19 0.4023423230568568 1107 20-21 0.6137244205209527 1107 22-23 0.502116370659536 1107 24-25 0.6180596108219305 1107 26-27 0.5394969268757137 1107 28-29 0.7548157865527756 1107 30-31 0.5855350826567829 1107 32-33 0.8157708881319721 1107 34-35 0.5980872650691822 1107 36-37 0.6991193964676157 1107 38-39 0.7954605289900272 1107 40-41 0.6918519883286862 1107 42-43 1.1116427861690212 1107 44-45 1.013407059710886 1107 46-47 1.2641179989788824 1107 48-49 1.277814109243728 1107 50-51 1.1288521613529312 1107 52-53 1.1561833802043822 1107 54-55 1.2513339490339632 1107 56-57 1.2969005242145002 1107 58-59 1.6062279263753751 1107 60-61 1.3382300344675784 1107 62-63 1.2304134877492885 1107 64-65 1.1981944773370614 1107 66-67 1.2590102129159817 1107 68-69 1.5958655571792946 1107 70-71 1.3314795650784461 1107 72-73 1.4287284751524787 1107 74-75 1.3737834379507028 1107 76-77 1.482627728538958 1107 78-79 1.594331163708727 1107 80-81 1.5638475633717874 1107 82-83 1.7848356790778332 1107 84-85 1.994814479683754 1107 86-87 2.2382662917029563 1107 88-89 2.0124216230184118 1107 90-91 2.281322867788102 1107 92-93 2.105954279481942 1107 94-95 2.3021478628189804 1107 96-97 2.1471948148395192 1107 98-99 2.5124292535271238 1107 100-101 2.3838079892160664 1108 1 0.4766924230749865 1108 2 0.5060292230482126 1108 3 0.5397518036747968 1108 4 0.422816791004756 1108 5 0.5248001439444465 1108 6 0.3763486453994318 1108 7 0.33918275629425665 1108 8 0.2622643829672242 1108 9 0.5044289378037377 1108 10-11 0.49682463351367545 1108 12-13 0.6115023702492621 1108 14-15 0.6108828265899859 1108 16-17 0.786214285147075 1108 18-19 0.620807899338736 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-1.4027447558218924 2316 96-97 -1.6030215009149238 2316 98-99 -1.29456717448965 2316 100-101 -1.0194489495277494 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3562.0 3 719.0 4 697.0 5 803.0 6 914.0 7 988.0 8 1207.0 9 1440.0 10 1814.0 11 2409.0 12 2943.0 13 3804.0 14 4315.0 15 5127.0 16 6820.0 17 9031.0 18 8020.0 19 5604.0 20 5503.0 21 5940.0 22 6360.0 23 7015.0 24 7746.0 25 8928.0 26 10237.0 27 12067.0 28 14316.0 29 17459.0 30 20968.0 31 26584.0 32 33821.0 33 44454.0 34 60855.0 35 86111.0 36 125471.0 37 171950.0 38 131356.0 39 16221.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.89143101144644 19.070051469946787 15.367061988916284 22.671455529690494 2 14.798393574297188 19.75915088927137 42.01491681009754 23.427538726333907 3 18.965378763714135 24.404104889472272 30.160198084782674 26.47031826203092 4 12.457515289905793 16.458708648406752 37.90852448106117 33.17525158062629 5 13.38810010407905 37.1411883371794 34.83641688077269 14.634294677968866 6 31.306482985145834 36.12789353444717 17.956887139541074 14.608736340865928 7 27.95596456523609 32.852154468048504 21.182534904389207 18.009346062326195 8 25.47917588857111 35.382755860544165 20.265636718508105 18.872431532376623 9 25.779988439518498 17.37501376247798 21.05762349530241 35.78737430270111 10-11 24.423135703393946 26.93847449880461 27.70967225815627 20.928717539645174 12-13 25.086246642459496 24.284737784975192 28.490924605295454 22.138090967269857 14-15 22.256202636174134 25.672792125311457 26.938350685135752 25.132654553378654 16-17 21.297794484433645 29.082577539358816 27.588553749192986 22.031074227014553 18-19 21.204581904379094 27.591541470110798 30.12979998176596 21.074076643744146 20-21 22.05639358548765 26.46932351777636 30.30174810941594 21.172534787320043 22-23 21.76443942408587 26.318713768843065 30.363185990906018 21.553660816165046 24-25 21.53236164308158 26.570319488085136 30.283307722299945 21.614011146533336 26-27 21.253919070477902 26.59922157570142 30.44575914717357 21.701100206647112 28-29 21.171830068470097 26.882640958317587 30.489348172248082 21.456180800964237 30-31 21.44233696845705 26.50649110121944 30.399087616248366 21.652084314075147 32-33 21.23037137065676 26.559725220692165 30.389375811737185 21.820527596913895 34-35 21.60920687017561 26.736322550088822 30.146983776394755 21.50748680334082 36-37 21.547055724027057 26.443928805071685 30.34121068990369 21.667804780997564 38-39 21.238245008338787 26.868200192746606 30.2521013221087 21.64145347680591 40-41 21.486035985679113 26.794511280997302 30.14220563490903 21.577247098414553 42-43 21.2516871378459 26.618362252146984 30.148938292897448 21.981012317109673 44-45 21.40176295411205 26.79876017563032 29.982661723576044 21.81681514668159 46-47 21.51631143377096 27.088137587336607 29.50644281279779 21.889108166094648 48-49 21.625844793161146 27.005455531739454 29.483266988470785 21.885432686628615 50-51 21.62664885336422 27.295464775245627 29.26138823347276 21.816498137917392 52-53 21.620772255640002 27.509865238632397 28.95055423687698 21.91880826885062 54-55 21.65339568249629 27.218895557507473 29.06460201954412 22.063106740452117 56-57 21.650749316497002 27.584742046054544 28.969749295285336 21.79475934216312 58-59 21.758266580746362 27.258102613613172 29.183799129942233 21.799831675698236 60-61 21.682894461530523 27.248020132593076 29.39931840395314 21.669767001923258 62-63 21.41064403802474 27.26243423006321 29.5152659633626 21.811655768549443 64-65 21.420836140054174 27.286486176601265 29.369010935462143 21.92366674788242 66-67 21.43045499624515 27.28446867422223 29.106746693724528 22.178329635808094 68-69 21.237980197315956 27.252109574622384 29.297160078862174 22.212750149199483 70-71 21.453874788377668 27.17422773973879 29.21371375761568 22.15818371426786 72-73 21.425606686884784 27.297498724423985 29.08181458571011 22.195080002981122 74-75 21.354199514944412 27.64381095445867 28.83586659251317 22.166122938083742 76-77 21.341711962363817 27.37979417283588 28.889243062606383 22.389250802193917 78-79 21.588589099927937 27.23619116089821 28.87731929387631 22.297900445297543 80-81 21.440016877840325 27.527161341031555 28.74071475131271 22.292107029815416 82-83 21.528525357720586 27.411697672365666 28.758545340317497 22.301231629596256 84-85 21.47407218459722 27.33596819819381 28.869711760305638 22.320247856903336 86-87 21.302676018553974 27.350487122047635 29.004225775164915 22.34261108423348 88-89 21.393431251941884 27.309248458819496 28.75418041987065 22.54313986936797 90-91 21.377521332977903 27.189091732521575 28.888334723524032 22.545052210976486 92-93 21.434287614221343 27.13480698455647 28.95174327283567 22.479162128386513 94-95 21.252440664479863 27.245955920710934 28.894945087198938 22.606658327610262 96-97 21.157487587045466 27.24996803961597 28.9603502055497 22.632194167788864 98-99 21.281638493873537 27.310753820010596 28.760270972506014 22.647336713609857 100-101 21.41962859733985 26.781027898193894 29.161770084395105 22.637573420071153 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 916.0 1 547.5 2 165.5 3 162.0 4 147.0 5 140.5 6 224.0 7 478.0 8 817.0 9 1046.5 10 1067.0 11 1066.0 12 1158.0 13 1231.5 14 1323.0 15 1402.5 16 1419.0 17 1456.0 18 1550.0 19 1644.0 20 1782.0 21 2099.0 22 2552.5 23 3102.5 24 3708.5 25 4455.5 26 5738.0 27 7001.0 28 7982.5 29 9386.0 30 11119.0 31 12613.0 32 14137.0 33 15949.5 34 18023.0 35 20026.0 36 22589.5 37 25194.0 38 26592.0 39 27447.5 40 28080.5 41 29423.0 42 30594.0 43 31708.5 44 33691.5 45 34990.0 46 36053.5 47 37092.0 48 36740.0 49 35322.5 50 33236.5 51 30457.0 52 28225.5 53 27270.5 54 26202.0 55 23579.0 56 20391.0 57 17665.5 58 15491.0 59 13265.0 60 10695.0 61 8528.0 62 6714.0 63 5070.0 64 3823.5 65 2884.0 66 2077.0 67 1557.0 68 1137.0 69 764.5 70 544.0 71 390.0 72 278.0 73 184.0 74 129.5 75 112.0 76 79.0 77 40.0 78 24.5 79 15.0 80 8.5 81 6.5 82 11.5 83 9.0 84 3.0 85 2.5 86 2.0 87 1.5 88 2.0 89 3.5 90 4.0 91 2.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.274272422571842 2 0.2385574827377791 3 0.23329181853147496 4 0.2397021923478452 5 0.24393761790508983 6 0.18773237605084342 7 0.17846022820930788 8 0.17926152493635417 9 0.18864814373889632 10-11 0.19385657246469715 12-13 0.19070862103701533 14-15 0.19191056612758475 16-17 0.17662869283320207 18-19 0.18183712155900292 20-21 0.17159197054891115 22-23 0.1807496474294401 24-25 0.1790325830143409 26-27 0.17926152493635417 28-29 0.1828673602080624 30-31 0.18115029579296324 32-33 0.184469953662155 34-35 0.18120753127346653 36-37 0.17485439293759958 38-39 0.1664980127841169 40-41 0.16386518068096484 42-43 0.17685763475521532 44-45 0.17456821553508306 46-47 0.17479715745709629 48-49 0.18601531163574422 50-51 0.18046347002692356 52-53 0.19568810784080293 54-55 0.1723932672759574 56-57 0.16283494203190535 58-59 0.16689866114764007 60-61 0.15642456821553508 62-63 0.16197640982435574 64-65 0.16272047107089874 66-67 0.15785545522811775 68-69 0.1636362387589516 70-71 0.16626907086210369 72-73 0.16357900327844832 74-75 0.17388138976904338 76-77 0.1653533031740508 78-79 0.16832954816022272 80-81 0.16420859356398468 82-83 0.1516740233337607 84-85 0.14955631055513838 86-87 0.1508727266067144 88-89 0.15602391985201194 90-91 0.1604310518507665 92-93 0.1572831004230847 94-95 0.15831333907214418 96-97 0.161461290499826 98-99 0.16781442883569295 100-101 0.1576265133061045 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 873584.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.41935822624474 #Duplication Level Percentage of deduplicated Percentage of total 1 83.18451103953467 54.41877326570328 2 10.104221250382308 13.220233391519892 3 2.8291579435989362 5.552450909727741 4 1.208447198176441 3.162233606200254 5 0.6588252642101482 2.154996298393201 6 0.4130397236733423 1.621247618679331 7 0.2810986658794116 1.2872506024059294 8 0.19424549960040102 1.0165932737755612 9 0.1555718138306552 0.9159667397004858 >10 0.897790305727104 10.900933276059632 >50 0.05178187453300592 2.3820599621832983 >100 0.020076644479791687 2.0713719181132277 >500 5.283327316503064E-4 0.2206283515634423 >1k 7.044436422004084E-4 1.0752607859747294 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3620 0.4143848788439349 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2864 0.3278448323229363 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1815 0.20776479422700048 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1034 0.11836297368083666 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 5.723548050330592E-5 0.0 22-23 0.0 0.0 0.0 2.8617740251652964E-4 0.0 24-25 0.0 0.0 0.0 6.295902855363651E-4 0.0 26-27 0.0 0.0 0.0 8.585322075495888E-4 0.0 28-29 0.0 0.0 0.0 0.002232183739628931 0.0 30-31 0.0 0.0 0.0 0.005208428725800839 0.0 32-33 0.0 0.0 0.0 0.00841361563398597 0.0 34-35 0.0 0.0 0.0 0.010931976776131431 0.0 36-37 0.0 0.0 0.0 0.016769995787468635 0.0 38-39 0.0 0.0 0.0 0.02501190497994469 0.0 40-41 0.0 0.0 0.0 0.04138125240389018 0.0 42-43 0.0 0.0 0.0 0.05694930310078939 0.0 44-45 0.0 0.0 0.0 0.07675277935493324 0.0 46-47 0.0 0.0 0.0 0.11223877726698292 0.0 48-49 0.0 0.0 0.0 0.13845262733749703 0.0 50-51 0.0 0.0 0.0 0.16661248374512355 0.0 52-53 0.0 0.0 0.0 0.20284254290371617 0.0 54-55 0.0 0.0 0.0 0.24960393047491714 0.0 56-57 0.0 0.0 0.0 0.35548956940603305 0.0 58-59 0.0 1.1447096100661185E-4 0.0 0.4432315610176011 0.0 60-61 0.0 1.1447096100661185E-4 0.0 0.5163212696203228 0.0 62-63 0.0 1.1447096100661185E-4 0.0 0.6028040806608179 0.0 64-65 0.0 1.1447096100661185E-4 0.0 0.7023365812560669 0.0 66-67 0.0 1.1447096100661185E-4 0.0 0.8383280829319217 0.0 68-69 0.0 1.1447096100661185E-4 0.0 0.992348760966318 0.0 70-71 0.0 1.1447096100661185E-4 0.0 1.097318632209381 0.0 72-73 0.0 1.1447096100661185E-4 0.0 1.2252399311342699 0.0 74-75 0.0 1.1447096100661185E-4 0.0 1.4215003937801058 0.0 76-77 0.0 1.1447096100661185E-4 0.0 1.6507857286763494 0.0 78-79 0.0 1.1447096100661185E-4 0.0 1.8736606897562225 0.0 80-81 0.0 1.1447096100661185E-4 0.0 2.055383340354219 0.0 82-83 0.0 1.1447096100661185E-4 0.0 2.3188382571109365 0.0 84-85 0.0 1.1447096100661185E-4 0.0 2.662537317533288 0.0 86-87 0.0 1.1447096100661185E-4 0.0 2.9673734866938957 0.0 88-89 0.0 1.1447096100661185E-4 0.0 3.345585541859741 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 2230 0.0 44.135845 1 GTATCAA 3725 0.0 38.42078 1 GGACCGA 90 5.692418E-7 36.9473 6 ATCAACG 4595 0.0 31.025045 3 TATCAAC 4630 0.0 30.89315 2 TCAACGC 4670 0.0 30.528538 4 CAACGCA 4690 0.0 30.499678 5 AACGCAG 4860 0.0 29.420998 6 TATGACG 70 0.008370897 27.155859 4 CGCAGAG 5470 0.0 25.359903 8 ACTGCGC 150 1.0583753E-6 25.336746 8 ACGCAGA 5625 0.0 25.252287 7 GTACACG 95 0.0012192198 25.024902 1 GTACATG 3195 0.0 23.662043 1 TACATGG 3125 0.0 23.569933 2 ACATGGG 3105 0.0 23.10958 3 GAGTACT 3240 0.0 23.018755 12-13 GCAGAGT 5890 0.0 22.904995 9 GTGCGGA 105 0.0021831298 22.641577 1 AGGACCG 175 4.030615E-6 21.724688 5 >>END_MODULE