FastQCFastQC Report
Thu 26 May 2016
SRR937682_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937682_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873584
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49970.5720113921500394No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40820.46727046282898954No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26550.30392040147255445No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11310.129466656898478No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10830.12397205077016062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32650.042.2036741
GTATCAA49350.036.0097351
ATCAACG61800.028.5950243
TATCAAC61950.028.5257852
TCAACGC62000.028.5027834
CAACGCA63500.027.9042995
AACGCAG65050.027.3807546
GTATAGG2301.3460522E-1024.7907051
CGCAGAG73350.024.2204728
ACGCAGA73550.024.1546127
TACATGG34900.024.092612
GTACATG35650.023.9910051
GAGTACT45900.023.13001812-13
GCAGAGT77800.022.5298299
ACATGGG37100.022.4078483
GTACTTT47000.022.3839814-15
ACCGTGC1100.002876145521.591788
AGTACTT46950.020.38687112-13
CAGAGTA75050.020.1906910-11
TACCTGG6500.019.7326972
Produced by FastQC (version 0.11.5)