FastQCFastQC Report
Thu 26 May 2016
SRR937681_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937681_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775400
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34080.4395150889863296No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27650.3565901470208925No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15750.20312096982202732No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9780.12612844983234459No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36650.036.1840061
GGACCGA1601.8189894E-1235.6329046
GGTATCA23850.034.6774641
AGGACCG2000.030.8858415
ATCAACG44600.029.08723
TCAACGC45300.028.7426284
ACCGAGT2151.8189894E-1228.72738
TATCAAC45450.028.545062
TGGACCG1004.9392107E-528.5100065
CAACGCA45700.028.2812615
AACGCAG47550.027.277346
GGACCGT700.00838083327.1488786
GTACATG28100.026.0495871
TACATGG27150.025.9056242
ACATGGG27300.025.0653633
ACGCAGA52800.024.1151967
CGCAGAG52550.024.0490998
AGTACTT29500.022.62205112-13
GCAGAGT55100.022.2434379
GAGTACT29150.022.24189412-13
Produced by FastQC (version 0.11.5)