##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937681_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 775400 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.90882641217436 33.0 31.0 34.0 30.0 34.0 2 32.056080732525146 33.0 31.0 34.0 30.0 34.0 3 32.07950219241682 34.0 31.0 34.0 30.0 34.0 4 35.651724271343824 37.0 35.0 37.0 35.0 37.0 5 35.63894119164302 37.0 35.0 37.0 35.0 37.0 6 35.68877740521022 37.0 35.0 37.0 35.0 37.0 7 35.64323316997679 37.0 35.0 37.0 33.0 37.0 8 35.618331183905084 37.0 35.0 37.0 33.0 37.0 9 37.278538818674235 39.0 37.0 39.0 34.0 39.0 10-11 37.163063580087695 39.0 37.0 39.0 34.0 39.0 12-13 37.04614070157338 39.0 37.0 39.0 33.0 39.0 14-15 38.246082667010576 40.0 38.0 41.0 33.5 41.0 16-17 38.18019409337116 40.0 38.0 41.0 33.0 41.0 18-19 38.11481171008512 40.0 38.0 41.0 33.5 41.0 20-21 38.033463373742585 40.0 38.0 41.0 33.0 41.0 22-23 37.84303778694867 40.0 38.0 41.0 33.0 41.0 24-25 37.684806551457314 40.0 37.5 41.0 32.5 41.0 26-27 37.61945576476657 40.0 37.5 41.0 32.5 41.0 28-29 37.656374129481556 40.0 37.5 41.0 33.0 41.0 30-31 37.48736587567707 40.0 37.0 41.0 32.5 41.0 32-33 37.25980719628579 40.0 37.0 41.0 32.0 41.0 34-35 37.315812483879284 40.0 37.0 41.0 32.0 41.0 36-37 37.25132834665979 40.0 37.0 41.0 32.0 41.0 38-39 37.1343571060098 40.0 37.0 41.0 31.0 41.0 40-41 36.91030629352592 40.0 36.5 41.0 31.0 41.0 42-43 36.66836278050039 39.0 36.0 41.0 30.0 41.0 44-45 36.445568093887026 39.0 36.0 41.0 30.0 41.0 46-47 36.10334085633222 39.0 36.0 41.0 29.5 41.0 48-49 35.75481299974207 39.0 35.0 41.0 28.0 41.0 50-51 34.8529578282177 38.0 34.0 39.5 26.5 40.5 52-53 34.954669202992 38.5 35.0 40.0 26.5 40.5 54-55 35.31024181067836 39.0 35.0 40.0 27.0 41.0 56-57 35.10609040495228 39.0 35.0 40.0 26.0 41.0 58-59 34.867981042042814 38.0 34.5 40.0 26.0 41.0 60-61 34.560526180036106 38.0 34.0 40.0 25.0 41.0 62-63 34.21707828217694 38.0 34.0 40.0 23.5 41.0 64-65 33.86323188031983 37.5 34.0 40.0 23.0 41.0 66-67 33.438485942739234 37.0 33.0 39.5 22.0 41.0 68-69 32.95221434098529 36.0 32.5 39.0 20.0 41.0 70-71 32.517103430487495 36.0 32.0 39.0 18.5 40.0 72-73 32.05123291204539 35.0 31.5 38.5 17.5 40.0 74-75 31.582430358524633 35.0 31.0 37.5 12.5 39.5 76-77 31.082606396698477 35.0 31.0 37.0 8.0 39.0 78-79 30.53169331957699 34.5 30.0 36.5 7.0 39.0 80-81 29.999124967758576 34.0 30.0 36.0 2.0 37.5 82-83 29.464106912561256 34.0 29.0 35.5 2.0 37.0 84-85 28.989663399535722 34.0 29.0 35.0 2.0 37.0 86-87 28.508345370131543 34.0 28.5 35.0 2.0 36.0 88-89 28.035824735620324 34.0 27.0 35.0 2.0 36.0 90-91 27.55115811194222 33.0 26.5 35.0 2.0 35.5 92-93 26.999271988651017 33.0 25.5 35.0 2.0 35.0 94-95 26.362267861748776 32.5 25.0 35.0 2.0 35.0 96-97 25.625608073252515 32.0 22.0 34.0 2.0 35.0 98-99 24.678411142636058 32.0 10.0 34.0 2.0 35.0 100-101 22.30541784885221 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1387528420850792 1101 2 0.11426035068885199 1101 3 0.14737542257987002 1101 4 0.12369463444989748 1101 5 0.20590243781019524 1101 6 0.1961696494546672 1101 7 0.15522363727807686 1101 8 0.15143321154980782 1101 9 0.35859200520283707 1101 10-11 0.18844912071598685 1101 12-13 0.32928983379805743 1101 14-15 0.37807684216264903 1101 16-17 0.3716258442036988 1101 18-19 0.19963743873250905 1101 20-21 0.42219539929317307 1101 22-23 0.2480672898385805 1101 24-25 0.3988480306564597 1101 26-27 0.33330312733465917 1101 28-29 0.1961840203117049 1101 30-31 0.2618087478675406 1101 32-33 0.1471601938449254 1101 34-35 0.352947970340594 1101 36-37 0.2647068380978723 1101 38-39 0.4179512964615455 1101 40-41 0.6685069605233096 1101 42-43 0.4364079251192834 1101 44-45 0.46417949324635543 1101 46-47 0.4621583139075298 1101 48-49 0.41669736797923207 1101 50-51 0.276053077552632 1101 52-53 0.49817854046828813 1101 54-55 0.6045768690070972 1101 56-57 0.5663761043064497 1101 58-59 0.6177174677850132 1101 60-61 0.6642111219818148 1101 62-63 0.8044600766647463 1101 64-65 0.5483092105384557 1101 66-67 0.8264811041888009 1101 68-69 0.66508989546805 1101 70-71 0.7333713386214669 1101 72-73 0.8210394108009673 1101 74-75 0.776300344333432 1101 76-77 0.780069067559026 1101 78-79 0.7456217268268084 1101 80-81 0.7900358536543095 1101 82-83 0.6866459602833181 1101 84-85 0.7748435991875375 1101 86-87 0.8870838905272826 1101 88-89 0.7935085499911096 1101 90-91 1.0206702042509619 1101 92-93 1.0465594812396013 1101 94-95 1.0180127493582418 1101 96-97 0.9329143895575207 1101 98-99 0.7986879307405452 1101 100-101 1.5549069092389658 1102 1 0.11091359168485226 1102 2 0.10572611959364764 1102 3 0.1914915116654754 1102 4 0.21559947483034847 1102 5 0.22168193682139758 1102 6 0.150738111645083 1102 7 0.1454063286802736 1102 8 0.3251342427203525 1102 9 0.3526710145257468 1102 10-11 0.20979646980724453 1102 12-13 0.23389478718349466 1102 14-15 0.3050797379155412 1102 16-17 0.2790000084376203 1102 18-19 0.3056075748093505 1102 20-21 0.30382119974519384 1102 22-23 0.5647887913924023 1102 24-25 0.5511237275205687 1102 26-27 0.32220126981511754 1102 28-29 0.37941543334494554 1102 30-31 0.5042544819291308 1102 32-33 0.3529411054185232 1102 34-35 0.5170818341695806 1102 36-37 0.7147933575441456 1102 38-39 0.6427532434433587 1102 40-41 0.665624450399946 1102 42-43 0.8450386813110171 1102 44-45 0.7429398520372033 1102 46-47 0.9487124856751237 1102 48-49 1.069310019829686 1102 50-51 0.990232849659229 1102 52-53 0.8566971745204555 1102 54-55 1.1726373619913701 1102 56-57 1.0653902534730335 1102 58-59 1.0594124837940555 1102 60-61 1.1762855875623757 1102 62-63 1.1284224318770981 1102 64-65 1.0269603900280444 1102 66-67 1.154021950784525 1102 68-69 1.0506826419601865 1102 70-71 1.0814885106715622 1102 72-73 0.9732488938031345 1102 74-75 0.9445173963157281 1102 76-77 0.9480506924766239 1102 78-79 1.0664852733001098 1102 80-81 0.9335742635799171 1102 82-83 0.8844223516572711 1102 84-85 1.0285971894502737 1102 86-87 0.9067023221121779 1102 88-89 0.8679917875841028 1102 90-91 0.8311308173030376 1102 92-93 1.286249247225097 1102 94-95 1.3946121819790172 1102 96-97 1.3691960544977242 1102 98-99 1.7892208199014838 1102 100-101 2.3697847225923887 1103 1 0.29322040926563986 1103 2 0.2804696008439791 1103 3 0.33493210245195115 1103 4 0.223152894198833 1103 5 0.23732098585085737 1103 6 0.20230300824422187 1103 7 0.21802493554545777 1103 8 0.1755848033592713 1103 9 0.24561410438664666 1103 10-11 0.2882892238136918 1103 12-13 0.25572532613732335 1103 14-15 0.47127645077321745 1103 16-17 0.5042985089929672 1103 18-19 0.3602144994933312 1103 20-21 0.2583428385562101 1103 22-23 0.36377775711938654 1103 24-25 0.3288573380880848 1103 26-27 0.16281007211056675 1103 28-29 0.15380537490098334 1103 30-31 0.275561074725438 1103 32-33 0.35874913947998266 1103 34-35 0.3214697592766811 1103 36-37 0.38906247744682787 1103 38-39 0.6465919341399271 1103 40-41 0.8617017960920137 1103 42-43 0.8191223540248203 1103 44-45 0.8365543202235912 1103 46-47 0.793097171241314 1103 48-49 0.9099196485386329 1103 50-51 0.7527546669755623 1103 52-53 0.8438720634504833 1103 54-55 0.7072748309182231 1103 56-57 0.7257062078814513 1103 58-59 0.6841224126563787 1103 60-61 0.9290970462813704 1103 62-63 0.9808299396784435 1103 64-65 0.8728879895943464 1103 66-67 0.887802759479726 1103 68-69 1.1644574674216628 1103 70-71 1.2746163865905302 1103 72-73 1.1233817810935598 1103 74-75 1.0871208302290931 1103 76-77 1.0566191129029257 1103 78-79 1.1588716910289953 1103 80-81 1.0915787394354162 1103 82-83 1.1273647803873708 1103 84-85 1.3163750296681833 1103 86-87 1.1546486845705282 1103 88-89 1.2970227020602216 1103 90-91 1.6179805951630932 1103 92-93 1.552651075912884 1103 94-95 1.3415175939957322 1103 96-97 1.516132374379243 1103 98-99 1.6271925367259392 1103 100-101 2.0347467736845744 1104 1 0.13813399390525305 1104 2 0.23228828782248456 1104 3 0.1664863929103504 1104 4 0.22687021526775197 1104 5 0.07812739743352637 1104 6 -0.1487526670123671 1104 7 0.10254528581923239 1104 8 -0.018168340165559016 1104 9 0.14533868219341883 1104 10-11 0.1778573337014393 1104 12-13 0.1483622849843229 1104 14-15 0.34077926319839946 1104 16-17 0.34308208293636255 1104 18-19 0.37300878283555505 1104 20-21 0.24196246601146498 1104 22-23 0.1286305687758471 1104 24-25 0.2125820608538973 1104 26-27 0.1683717774439586 1104 28-29 -0.06878418672967257 1104 30-31 0.18044487686952237 1104 32-33 0.3061992878433699 1104 34-35 0.3237995367206139 1104 36-37 0.3266713340054608 1104 38-39 0.48750377048555293 1104 40-41 0.47998947830664207 1104 42-43 0.422957509229839 1104 44-45 0.5311475673282544 1104 46-47 0.37319473307403683 1104 48-49 0.31783221626855607 1104 50-51 0.3506311267241742 1104 52-53 0.38569823997985964 1104 54-55 0.35872354286922103 1104 56-57 0.46029936713215136 1104 58-59 0.27434569351893145 1104 60-61 0.4868319293587149 1104 62-63 0.5553218691932003 1104 64-65 0.5516977220570496 1104 66-67 0.6368144023269835 1104 68-69 0.590213176282397 1104 70-71 0.6916772979436843 1104 72-73 0.6944644275186711 1104 74-75 0.831359257115917 1104 76-77 0.9696409380252042 1104 78-79 1.037805077953248 1104 80-81 0.9353776768416644 1104 82-83 0.9530762328111493 1104 84-85 0.8379695352809549 1104 86-87 1.0052359883541762 1104 88-89 0.9941615179204959 1104 90-91 1.279354609276833 1104 92-93 1.256850056367572 1104 94-95 1.5109922931507924 1104 96-97 1.4374574977591656 1104 98-99 1.6974823010796314 1104 100-101 2.3863372599782586 1105 1 0.14481125339150225 1105 2 0.07718072026172962 1105 3 0.13154427510306732 1105 4 0.15336518998370252 1105 5 0.10049684723743013 1105 6 0.14532891030891193 1105 7 0.14601367760223383 1105 8 0.15546562747589832 1105 9 0.09659752530619414 1105 10-11 0.2837981400167777 1105 12-13 0.22844762653971173 1105 14-15 0.3954323798833528 1105 16-17 0.41437238924539344 1105 18-19 0.4185697293796551 1105 20-21 0.4437154830734684 1105 22-23 0.34297832221536595 1105 24-25 0.3118329971834939 1105 26-27 0.30291319662957505 1105 28-29 0.2908136644412167 1105 30-31 0.4114534646196759 1105 32-33 0.36856136050506905 1105 34-35 0.4341027877073813 1105 36-37 0.22649431411870324 1105 38-39 0.5594088386453038 1105 40-41 0.4320845551676342 1105 42-43 0.5037845057871735 1105 44-45 0.3506968365498082 1105 46-47 0.27251486951963955 1105 48-49 0.4679491786514305 1105 50-51 0.17365222408983527 1105 52-53 0.40423646240385125 1105 54-55 0.5053079890791352 1105 56-57 0.4327509428140601 1105 58-59 0.3732742739550048 1105 60-61 0.6325659550084737 1105 62-63 0.8470965132618531 1105 64-65 0.6272251561987119 1105 66-67 0.8586890476566822 1105 68-69 0.7348374779759794 1105 70-71 0.7979609522142255 1105 72-73 0.837183755631262 1105 74-75 0.7869245858085883 1105 76-77 1.0824978151611084 1105 78-79 1.2523530230723559 1105 80-81 1.3591692757268952 1105 82-83 1.3392317959330065 1105 84-85 1.1813786577162944 1105 86-87 1.102615053663957 1105 88-89 1.1653080645352105 1105 90-91 1.0554228762158822 1105 92-93 1.4722925587950968 1105 94-95 1.4845520164764352 1105 96-97 2.0908784110115377 1105 98-99 2.0131522653919163 1105 100-101 2.6449707231997373 1106 1 -0.005607341012549227 1106 2 0.14403648878636943 1106 3 0.17183398649324033 1106 4 0.11693095336770654 1106 5 0.15787916345980335 1106 6 -0.030049293407031996 1106 7 0.1624086250943364 1106 8 -0.2811577109980732 1106 9 -0.1253584855625789 1106 10-11 -0.22383937191683856 1106 12-13 -0.07203614374743239 1106 14-15 -0.003925958957800901 1106 16-17 -0.1453232118414789 1106 18-19 0.009223874932999365 1106 20-21 0.18504525468248545 1106 22-23 -0.014236005463281742 1106 24-25 -0.1166663051591712 1106 26-27 0.08909040159395687 1106 28-29 0.21184068948343793 1106 30-31 0.5125403413179228 1106 32-33 0.41996769082746255 1106 34-35 0.3442656356180862 1106 36-37 0.18438243191263126 1106 38-39 0.5344767684389495 1106 40-41 0.6611227060084914 1106 42-43 0.6879109653597695 1106 44-45 0.6535397902106794 1106 46-47 0.7854628389695861 1106 48-49 1.10589255825046 1106 50-51 0.9982923797775811 1106 52-53 0.9446868185762796 1106 54-55 1.003958572908175 1106 56-57 1.116468905654628 1106 58-59 1.3055234845006467 1106 60-61 1.3293007350628159 1106 62-63 1.4771958376357972 1106 64-65 1.45973231636615 1106 66-67 1.3660924771932912 1106 68-69 1.470172904281334 1106 70-71 1.627819768399874 1106 72-73 1.51903364914671 1106 74-75 1.9836088775027996 1106 76-77 1.9436869537378918 1106 78-79 1.7919313075696728 1106 80-81 1.861344865063753 1106 82-83 1.3669453157978815 1106 84-85 1.5641446854757106 1106 86-87 1.5633580587311506 1106 88-89 1.5307611746181955 1106 90-91 1.4666689289440917 1106 92-93 1.58982028149466 1106 94-95 2.052642454898997 1106 96-97 1.8667009122966967 1106 98-99 2.2977826905957066 1106 100-101 2.42069224776462 1107 1 0.197263410312555 1107 2 0.24919543957525292 1107 3 0.07735309394718826 1107 4 -0.010084503098433117 1107 5 0.08094278084121953 1107 6 0.2174147835911313 1107 7 0.2474346329770114 1107 8 0.31527531715963164 1107 9 0.19084732883627709 1107 10-11 0.2081200533151346 1107 12-13 0.05128353699191024 1107 14-15 0.17705739846834234 1107 16-17 0.28695899530729463 1107 18-19 0.20018412796931528 1107 20-21 0.1531855984066155 1107 22-23 0.04414891120030262 1107 24-25 0.1098725460460841 1107 26-27 -0.08294309359366281 1107 28-29 0.16701106891434847 1107 30-31 0.13100593801659244 1107 32-33 0.2047107625993334 1107 34-35 0.5832539845808569 1107 36-37 0.49910561166972656 1107 38-39 0.2906698568904531 1107 40-41 0.5277056655943468 1107 42-43 0.773319987191563 1107 44-45 0.9038007956840701 1107 46-47 0.4889065668072945 1107 48-49 0.31439732121425124 1107 50-51 0.3817032425473883 1107 52-53 0.35808860020890876 1107 54-55 0.40329231396603404 1107 56-57 0.4105803750691095 1107 58-59 0.4814535288561288 1107 60-61 0.5507986284861062 1107 62-63 0.6615691773938792 1107 64-65 0.4286896535543363 1107 66-67 0.3463101156034156 1107 68-69 0.4253880716337193 1107 70-71 0.7062665850597512 1107 72-73 0.9086669441786519 1107 74-75 0.8276496128895836 1107 76-77 0.6085420262016576 1107 78-79 1.2718446969960162 1107 80-81 1.1937469185127334 1107 82-83 1.2264208955698024 1107 84-85 1.1575367682067252 1107 86-87 1.0200512274920364 1107 88-89 0.9894250119237533 1107 90-91 1.19603559684629 1107 92-93 1.3811703438888685 1107 94-95 1.0719028055420878 1107 96-97 1.3706329829195276 1107 98-99 1.784059053692932 1107 100-101 2.0867704938078404 1108 1 0.3860424223410668 1108 2 0.2633175345380039 1108 3 0.2604073468718724 1108 4 0.18944616634945532 1108 5 0.09071666627701802 1108 6 0.2570816509247251 1108 7 0.20269485452863734 1108 8 0.38877692952480203 1108 9 0.46462348423683864 1108 10-11 0.502101579176248 1108 12-13 0.38787483213717167 1108 14-15 0.4959050079117091 1108 16-17 0.5366972975116866 1108 18-19 0.5146892794856299 1108 20-21 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-0.8650360844066363 2316 96-97 -0.2776445934897147 2316 98-99 -0.12455046284916094 2316 100-101 0.4739617206914062 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3234.0 3 623.0 4 552.0 5 616.0 6 726.0 7 825.0 8 941.0 9 1161.0 10 1502.0 11 1917.0 12 2513.0 13 3220.0 14 3646.0 15 4679.0 16 5840.0 17 7986.0 18 7074.0 19 4991.0 20 4733.0 21 5132.0 22 5368.0 23 5826.0 24 6660.0 25 7433.0 26 8587.0 27 10227.0 28 11754.0 29 14481.0 30 17746.0 31 22174.0 32 28743.0 33 38201.0 34 52378.0 35 74600.0 36 112129.0 37 156251.0 38 124878.0 39 16046.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8802540868173 19.108368389675654 15.31348969776756 22.69788782573948 2 14.738566443466713 19.722747647867276 42.02214243509898 23.51654347356703 3 18.960598080773998 24.417710244188736 30.11100898336352 26.510682691673743 4 12.416574564209954 16.56137901615186 37.77700217814231 33.245044241495876 5 13.431361904318736 37.271150336326926 34.65408219716358 14.643405562190754 6 31.20488839845254 36.15401711301256 17.999545169101477 14.641549319433425 7 27.811098908198208 32.910136313715356 21.185864719943147 18.09290005814329 8 25.452304066181576 35.47645537822195 20.163567312898405 18.907673242698074 9 25.790253092912724 17.3107488893426 21.074449581710528 35.82454843603415 10-11 24.410736992277553 26.930740920697605 27.64748374357755 21.011038343447293 12-13 25.05215740066511 24.298338393468065 28.546137897124733 22.10336630874209 14-15 22.342961772246113 25.612750165398612 26.90105028117764 25.14323778117764 16-17 21.24174244504385 29.112335050797082 27.631224460129538 22.014698044029526 18-19 21.160759048785657 27.468291132370954 30.260713048961385 21.110236769882 20-21 21.878510643933254 26.449978714071516 30.502574014629474 21.16893662736576 22-23 21.73053059415771 26.263890799886568 30.4581162063154 21.547462399640324 24-25 21.53976344127698 26.47183840106458 30.403157561545964 21.585240596112477 26-27 21.190915797865262 26.692240803453416 30.48223484706554 21.634608551615784 28-29 21.132986847852212 26.90110432555507 30.52543980482409 21.440469021768628 30-31 21.435031986220924 26.526339047481223 30.475871755346436 21.562757210951418 32-33 21.137923526359895 26.568455221098514 30.509210472757538 21.78441077978406 34-35 21.533998772490875 26.574861905223372 30.35953096230255 21.531608359983203 36-37 21.4356726978391 26.489247829314095 30.37442192022935 21.700657552617454 38-39 21.231420996599446 26.868430164652864 30.285132146068694 21.615016692678992 40-41 21.41102891589302 26.84622718781248 30.298770861378898 21.443973034915597 42-43 21.279034160724276 26.605026881268905 30.18829162785109 21.92764733015573 44-45 21.330660608029934 26.73950553720551 30.084438911796834 21.84539494296772 46-47 21.530201637102312 27.016710175008836 29.60886420293815 21.844223984950702 48-49 21.53814149526962 27.049076536242104 29.502838743355376 21.909943225132892 50-51 21.549548571672243 27.276621478607066 29.35918757115149 21.814642378569204 52-53 21.544694434034195 27.465850790245668 29.04511443377573 21.944340341944404 54-55 21.579167694913632 27.19714304541126 29.190370181828516 22.033319077846585 56-57 21.590452556668083 27.524663711887026 29.025671962620443 21.859211768824448 58-59 21.665508296342995 27.21276722182318 29.287046749931534 21.834677731902293 60-61 21.631180018784388 27.24518020223578 29.3933055744677 21.73033420451213 62-63 21.2971193500708 27.311264199113168 29.528212177903647 21.863404272912383 64-65 21.417008912826383 27.261440754056892 29.437454541226504 21.88409579189022 66-67 21.356260658363908 27.268358224381167 29.19610097669371 22.17928014056121 68-69 21.151212516634153 27.20281391713285 29.374103693750726 22.271869872482267 70-71 21.396859192040687 27.11364386563166 29.336084280263268 22.153412662064387 72-73 21.430712326608024 27.22355792522618 29.18314404534127 22.16258570282453 74-75 21.271289085841083 27.597115052308247 28.94314405036062 22.18845181149005 76-77 21.26788652630722 27.367055520346177 28.961749692967746 22.403308260378854 78-79 21.521273956459716 27.1455503524985 28.986540966321407 22.346634724720378 80-81 21.345986750089637 27.443271196787975 28.878279772729478 22.33246228039291 82-83 21.438086427569168 27.38066013235724 28.870906473927793 22.3103469661458 84-85 21.42328354043767 27.355085102699462 28.916248711250304 22.305382645612568 86-87 21.16675721261459 27.359389464069196 29.085597897180715 22.3882554261355 88-89 21.29331038777477 27.24391025392568 28.83866533334367 22.62411402495588 90-91 21.320451468201878 27.27122975473122 28.93510144597989 22.473217331087017 92-93 21.36431348279912 27.02574027682135 29.13097011502569 22.478976125353846 94-95 21.115058116587175 27.170643064736705 29.051446083323807 22.662852735352317 96-97 21.03593254146398 27.215433119895927 28.98806382581544 22.760570512824653 98-99 21.150897156152585 27.207298687305975 28.89070184753836 22.751102309003084 100-101 21.34469663938879 26.741039737336585 29.18112755879471 22.733136064479915 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 934.0 1 545.0 2 148.5 3 126.5 4 113.5 5 138.5 6 226.0 7 431.5 8 750.5 9 963.5 10 993.0 11 993.5 12 1023.0 13 1113.0 14 1226.0 15 1283.5 16 1278.5 17 1328.5 18 1398.0 19 1469.5 20 1608.5 21 1845.5 22 2195.0 23 2699.0 24 3320.5 25 4087.0 26 5160.5 27 6388.5 28 7272.5 29 8267.0 30 9775.5 31 11170.5 32 12528.0 33 14258.5 34 16283.0 35 17987.5 36 20102.0 37 22241.5 38 23473.0 39 24279.5 40 24939.5 41 26043.0 42 27225.5 43 28338.5 44 29627.5 45 31141.5 46 32269.0 47 32831.5 48 32518.5 49 31227.0 50 29342.0 51 26949.5 52 25211.0 53 24196.5 54 23057.0 55 20978.0 56 18084.5 57 15529.0 58 13627.5 59 11641.0 60 9406.5 61 7542.0 62 5934.5 63 4445.0 64 3395.5 65 2573.0 66 1818.0 67 1315.5 68 957.0 69 650.5 70 465.5 71 344.0 72 248.0 73 174.5 74 120.5 75 91.0 76 56.0 77 32.0 78 22.5 79 13.0 80 9.5 81 10.0 82 8.5 83 5.5 84 4.0 85 4.5 86 4.5 87 2.5 88 1.0 89 0.0 90 0.0 91 0.5 92 1.5 93 1.5 94 2.5 95 2.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.27353623936033017 2 0.2518700025793139 3 0.23974722723755482 4 0.2327830797007996 5 0.22543203507866907 6 0.19164302295589375 7 0.18700025793139025 8 0.1971885478462729 9 0.19783337632189837 10-11 0.20015475883415013 12-13 0.1986716533402115 14-15 0.19499613102914626 16-17 0.19183647149858138 18-19 0.19138509156564354 20-21 0.18242197575444932 22-23 0.17687645086407017 24-25 0.17861748774825895 26-27 0.18583956667526438 28-29 0.19783337632189837 30-31 0.1899664689192675 32-33 0.19673716791333506 34-35 0.19022440030951768 36-37 0.17971369615682228 38-39 0.1815836987361362 40-41 0.17565127676038172 42-43 0.18583956667526438 44-45 0.18164818158369875 46-47 0.18190611297394893 48-49 0.1890637090533918 50-51 0.1964147536755223 52-53 0.2050554552489038 54-55 0.1842919783337632 56-57 0.16597884962599949 58-59 0.17075058034562807 60-61 0.17449058550425586 62-63 0.1815836987361362 64-65 0.1737167913335053 66-67 0.17539334537013154 68-69 0.17926231622388444 70-71 0.17584472530306938 72-73 0.17526437967500644 74-75 0.17829507351044624 76-77 0.18809646633995358 78-79 0.1929326799071447 80-81 0.18474335826670107 82-83 0.1849368068093887 84-85 0.17977817900438484 86-87 0.1794557647665721 88-89 0.17977817900438484 90-91 0.17952024761413465 92-93 0.1804230074800103 94-95 0.1799716275470725 96-97 0.17081506319319062 98-99 0.17320092855300492 100-101 0.17145989166881612 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 775400.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.32986043245143 #Duplication Level Percentage of deduplicated Percentage of total 1 83.54700675203121 55.416612974113065 2 9.96965669204132 13.225718738851121 3 2.736777532788824 5.445902153536543 4 1.1663698672942717 3.0946060204098376 5 0.642524482315663 2.1309279618215515 6 0.40376877125963273 1.6069155746780306 7 0.285470530340584 1.325465430454818 8 0.1858110262002168 0.9859855547736764 9 0.1431591841990408 0.8546155836791384 >10 0.8458036320673233 10.302748598997008 >50 0.053388259282955146 2.3932873092985494 >100 0.019094235366659287 1.9292428756011941 >500 5.845174061560086E-4 0.28664661592979485 >1k 5.845174061560086E-4 1.0013246078557487 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3408 0.4395150889863296 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2765 0.3565901470208925 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1575 0.20312096982202732 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 978 0.12612844983234459 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.2896569512509673E-4 0.0 14-15 0.0 0.0 0.0 1.2896569512509673E-4 0.0 16-17 0.0 0.0 0.0 1.2896569512509673E-4 0.0 18-19 0.0 0.0 0.0 1.2896569512509673E-4 0.0 20-21 0.0 0.0 0.0 1.2896569512509673E-4 0.0 22-23 0.0 0.0 0.0 3.8689708537529015E-4 0.0 24-25 0.0 0.0 0.0 6.448284756254836E-4 0.0 26-27 0.0 0.0 0.0 6.448284756254836E-4 0.0 28-29 0.0 0.0 0.0 0.001418622646376064 0.0 30-31 0.0 0.0 0.0 0.003740005158627805 0.0 32-33 0.0 0.0 0.0 0.006448284756254836 0.0 34-35 0.0 0.0 0.0 0.009672427134382253 0.0 36-37 0.0 0.0 0.0 0.016249677585762186 0.0 38-39 0.0 0.0 0.0 0.02611555326283209 0.0 40-41 0.0 0.0 0.0 0.04371937064740779 0.0 42-43 0.0 0.0 0.0 0.06171008511735878 0.0 44-45 0.0 0.0 0.0 0.08060355945318545 0.0 46-47 0.0 0.0 0.0 0.12077637348465309 0.0 48-49 0.0 0.0 0.0 0.1476012380706732 0.0 50-51 0.0 0.0 0.0 0.1771988651018829 0.0 52-53 0.0 0.0 0.0 0.2144054681454733 0.0 54-55 0.0 0.0 0.0 0.26276760381738457 0.0 56-57 0.0 0.0 0.0 0.38373742584472526 0.0 58-59 0.0 0.0 0.0 0.48362135671911277 0.0 60-61 0.0 0.0 0.0 0.5655145731235491 0.0 62-63 0.0 0.0 0.0 0.6618519473819964 0.0 64-65 0.0 0.0 0.0 0.7635413979881351 0.0 66-67 0.0 0.0 0.0 0.903920557131803 0.0 68-69 0.0 0.0 0.0 1.0628062935259222 0.0 70-71 0.0 0.0 0.0 1.1713954088212535 0.0 72-73 0.0 0.0 0.0 1.3005545524890378 0.0 74-75 0.0 0.0 0.0 1.5125096724271345 0.0 76-77 0.0 0.0 0.0 1.766572091823575 0.0 78-79 0.0 0.0 0.0 2.007157596079443 0.0 80-81 0.0 0.0 0.0 2.1992519989682746 0.0 82-83 0.0 0.0 0.0 2.4833634253288626 0.0 84-85 0.0 0.0 0.0 2.847820479752386 0.0 86-87 0.0 0.0 0.0 3.1679133350528756 0.0 88-89 0.0 0.0 0.0 3.5577766314160435 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3665 0.0 36.184006 1 GGACCGA 160 1.8189894E-12 35.632904 6 GGTATCA 2385 0.0 34.677464 1 AGGACCG 200 0.0 30.885841 5 ATCAACG 4460 0.0 29.0872 3 TCAACGC 4530 0.0 28.742628 4 ACCGAGT 215 1.8189894E-12 28.7273 8 TATCAAC 4545 0.0 28.54506 2 TGGACCG 100 4.9392107E-5 28.510006 5 CAACGCA 4570 0.0 28.281261 5 AACGCAG 4755 0.0 27.27734 6 GGACCGT 70 0.008380833 27.148878 6 GTACATG 2810 0.0 26.049587 1 TACATGG 2715 0.0 25.905624 2 ACATGGG 2730 0.0 25.065363 3 ACGCAGA 5280 0.0 24.115196 7 CGCAGAG 5255 0.0 24.049099 8 AGTACTT 2950 0.0 22.622051 12-13 GCAGAGT 5510 0.0 22.243437 9 GAGTACT 2915 0.0 22.241894 12-13 >>END_MODULE