FastQCFastQC Report
Thu 26 May 2016
SRR937681_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937681_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences775400
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43450.5603559453185453No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37180.47949445447510963No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24080.3105493938612329No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10060.1297394892958473No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT9020.11632705700283724No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31250.037.5621031
GTATCAA47300.034.562111
GTACACG753.0688412E-431.6819341
TAGACGG650.005842041729.2316655
GACGGTC1303.0356205E-729.2297767
GGACCGA2002.3646862E-1128.4990316
ATCAACG57900.027.8117313
TATCAAC58550.027.6652362
TCAACGC59400.027.1094154
CAACGCA60000.026.9966585
AACGCAG61850.026.2642676
GTATAGG2301.3278623E-1024.794561
AGGACCG2701.8189894E-1224.6303835
GTACATG34750.024.3426691
TTAGTCG500.001652990823.75225638-39
TACATGG34400.023.7507252
ACGATTC1000.001653488823.7491938
ACGGTCC1000.001653488823.7491938
ACGCAGA68500.023.7145237
CGCAGAG68850.023.3870078
Produced by FastQC (version 0.11.5)