##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937681_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 775400 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.468838019086924 33.0 31.0 34.0 31.0 34.0 2 32.681089760123804 34.0 31.0 34.0 31.0 34.0 3 32.73864457054424 34.0 31.0 34.0 31.0 34.0 4 36.274365488779985 37.0 35.0 37.0 35.0 37.0 5 36.21696414753676 37.0 35.0 37.0 35.0 37.0 6 36.27347691514057 37.0 37.0 37.0 35.0 37.0 7 36.23305003868971 37.0 37.0 37.0 35.0 37.0 8 36.20455635800877 37.0 37.0 37.0 35.0 37.0 9 37.935531338663914 39.0 38.0 39.0 35.0 39.0 10-11 37.865698349239096 39.0 38.0 39.0 35.0 39.0 12-13 37.76570931132319 39.0 37.5 39.0 35.0 39.0 14-15 39.05381287077637 40.0 38.0 41.0 36.0 41.0 16-17 39.01040172814031 40.0 38.0 41.0 36.0 41.0 18-19 38.97421717823059 40.0 38.0 41.0 36.0 41.0 20-21 38.8791958988909 40.0 38.0 41.0 35.0 41.0 22-23 38.831516636574676 40.0 38.0 41.0 35.0 41.0 24-25 38.84957892700542 40.0 38.0 41.0 35.0 41.0 26-27 38.67801521795202 40.0 38.0 41.0 35.0 41.0 28-29 38.64451444415785 40.0 38.0 41.0 34.5 41.0 30-31 38.66346466339954 40.0 38.0 41.0 35.0 41.0 32-33 38.535559711116846 40.0 38.0 41.0 35.0 41.0 34-35 38.382950735104465 40.0 38.0 41.0 34.0 41.0 36-37 38.226123291204544 40.0 38.0 41.0 34.0 41.0 38-39 38.09223304101109 40.0 38.0 41.0 34.0 41.0 40-41 37.90923265411401 40.0 38.0 41.0 33.0 41.0 42-43 37.67678359556358 40.0 37.0 41.0 33.0 41.0 44-45 37.345432035078666 40.0 37.0 41.0 33.0 41.0 46-47 36.95177069899407 40.0 37.0 41.0 31.5 41.0 48-49 36.55754707247872 40.0 36.5 41.0 30.5 41.0 50-51 36.27907854010833 39.5 36.0 41.0 30.0 41.0 52-53 36.014999355171526 39.0 36.0 41.0 29.5 41.0 54-55 35.78532241423781 39.0 35.0 40.5 28.5 41.0 56-57 35.50514573123549 39.0 35.0 40.0 28.0 41.0 58-59 35.25553069383544 38.5 35.0 40.0 28.0 41.0 60-61 34.904457699252 38.0 34.5 40.0 26.5 41.0 62-63 34.50016378643281 38.0 34.0 40.0 26.0 41.0 64-65 34.1228333763219 37.0 34.0 40.0 26.0 41.0 66-67 33.693144828475624 37.0 33.5 39.0 24.5 41.0 68-69 33.21132512251741 36.0 33.0 39.0 22.5 40.0 70-71 32.77926038173845 35.5 32.5 39.0 22.0 40.0 72-73 32.311073639411916 35.0 32.0 38.0 20.5 40.0 74-75 31.819542171782306 35.0 31.5 37.0 19.5 39.0 76-77 30.589038560742843 34.0 30.5 36.0 18.5 38.5 78-79 31.161350915656435 34.5 31.0 36.0 18.0 39.0 80-81 30.972420041269025 34.5 31.0 36.0 18.0 37.5 82-83 30.585443641991233 34.0 31.0 35.5 13.5 37.0 84-85 30.180025793139023 34.0 31.0 35.0 7.5 37.0 86-87 29.82786432808873 34.0 30.0 35.0 4.5 36.0 88-89 29.43994841372195 34.0 30.0 35.0 2.0 36.0 90-91 28.95736265153469 34.0 29.0 35.0 2.0 35.5 92-93 28.508878643280887 34.0 29.0 35.0 2.0 35.0 94-95 28.030917590920815 34.0 29.0 35.0 2.0 35.0 96-97 27.538011993809647 33.0 27.0 35.0 2.0 35.0 98-99 26.777708279597626 33.0 26.0 35.0 2.0 35.0 100-101 24.33636961568223 30.5 20.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11964212555697173 1101 2 0.08329612999679625 1101 3 0.10944509288824378 1101 4 0.06018072952031872 1101 5 0.06815259572459098 1101 6 0.053234511443776 1101 7 0.041584835155063615 1101 8 0.07210083547892054 1101 9 0.21191030283088708 1101 10-11 0.05213318344451068 1101 12-13 0.03305423307634214 1101 14-15 0.1172704587556126 1101 16-17 0.11449832772049007 1101 18-19 0.18919103264403248 1101 20-21 0.19420339484018712 1101 22-23 0.11403243320901879 1101 24-25 0.046688541945727025 1101 26-27 0.12383816255145064 1101 28-29 0.09693066103942982 1101 30-31 0.27627917512314326 1101 32-33 0.25237312451249494 1101 34-35 0.18003250288433037 1101 36-37 0.06342492663747379 1101 38-39 0.17463151969716506 1101 40-41 0.11529247784709185 1101 42-43 0.1604288272374319 1101 44-45 0.3130165729457133 1101 46-47 -0.0359356401017763 1101 48-49 -0.24140632886584257 1101 50-51 -0.11071630213433536 1101 52-53 -0.012604110268803481 1101 54-55 0.049141035837017455 1101 56-57 0.09425164472022374 1101 58-59 0.046229890609787105 1101 60-61 0.21022171876255413 1101 62-63 0.0471169283546331 1101 64-65 0.09633301288365459 1101 66-67 0.1687621963016781 1101 68-69 0.4154199403305654 1101 70-71 0.12869460930217258 1101 72-73 3.301692677624146E-4 1101 74-75 0.131162317834292 1101 76-77 -0.08143417334026282 1101 78-79 -0.10724049028203453 1101 80-81 0.09929274434101742 1101 82-83 0.22377735284498712 1101 84-85 0.3029780941083473 1101 86-87 0.2709954162095123 1101 88-89 0.5371409758262331 1101 90-91 0.38084735395431935 1101 92-93 0.47135684720311133 1101 94-95 0.4768144128300449 1101 96-97 0.5289088715852763 1101 98-99 0.6906886241191721 1101 100-101 1.0782644760092701 1102 1 0.11668163021843014 1102 2 0.05740798139522951 1102 3 0.10901838133348463 1102 4 0.1739037731214097 1102 5 0.021120153875074266 1102 6 0.11855375054349793 1102 7 0.057473219183691526 1102 8 0.029473822577514852 1102 9 0.1935793630060445 1102 10-11 0.12423860768407735 1102 12-13 0.17065399529501235 1102 14-15 0.16571546299330464 1102 16-17 0.27116326792202017 1102 18-19 0.28542184533826287 1102 20-21 0.18115926403690708 1102 22-23 0.21390206547194168 1102 24-25 0.13393192644520724 1102 26-27 0.21138171655842086 1102 28-29 0.22940399892435437 1102 30-31 0.18120636930338918 1102 32-33 0.17398621190302066 1102 34-35 0.08336615012042614 1102 36-37 0.3992748771977901 1102 38-39 0.314985778572769 1102 40-41 0.24665783710879907 1102 42-43 0.2414819513545794 1102 44-45 0.1774797403814432 1102 46-47 0.06726087086912713 1102 48-49 0.22520128484542568 1102 50-51 0.1969118290456322 1102 52-53 0.38749977192349405 1102 54-55 0.3251969262221692 1102 56-57 0.36071241079989136 1102 58-59 0.37399292149781616 1102 60-61 0.2982964550842411 1102 62-63 0.5351837190128848 1102 64-65 0.4679840628883625 1102 66-67 0.3074743514010265 1102 68-69 0.35551846655714314 1102 70-71 0.34766090791785587 1102 72-73 0.4217990575811683 1102 74-75 0.6429998849310117 1102 76-77 0.5620615654298682 1102 78-79 0.49306909630804086 1102 80-81 0.5402491079345744 1102 82-83 0.599792632061483 1102 84-85 0.5977239505920089 1102 86-87 0.6813874611289954 1102 88-89 0.6712534746490988 1102 90-91 0.6292287928845433 1102 92-93 0.628847253550223 1102 94-95 0.7779888995990412 1102 96-97 0.9700535768305905 1102 98-99 1.0514497009625678 1102 100-101 1.3302082207121622 1103 1 0.07195943737979604 1103 2 0.12809112784892562 1103 3 0.16499136752906907 1103 4 0.0560043178931835 1103 5 0.13801799594414632 1103 6 0.05354475916464452 1103 7 0.11610395118771066 1103 8 0.13682328309478464 1103 9 0.16833243065294567 1103 10-11 0.16998555325979225 1103 12-13 0.2109276042758097 1103 14-15 0.2652586216117996 1103 16-17 0.1304283549268277 1103 18-19 0.18965043792301373 1103 20-21 0.3006660935180463 1103 22-23 0.29763345463997837 1103 24-25 0.09166252886759452 1103 26-27 0.11475745041360597 1103 28-29 0.08986655908725538 1103 30-31 0.10551763629445077 1103 32-33 0.09541164296030757 1103 34-35 0.24102601154740455 1103 36-37 0.31561455959055706 1103 38-39 0.31980955415853174 1103 40-41 0.21179145164309432 1103 42-43 0.3041331850246536 1103 44-45 0.5732070173159087 1103 46-47 0.6356432821453737 1103 48-49 0.6197757149506842 1103 50-51 0.6096252090107228 1103 52-53 0.7094795611549998 1103 54-55 0.7947617699308509 1103 56-57 0.6136122957631329 1103 58-59 0.5114940784767086 1103 60-61 0.6566681890980988 1103 62-63 0.4861592175055094 1103 64-65 0.47757376511505356 1103 66-67 0.5016162366816133 1103 68-69 0.6243061510126324 1103 70-71 0.5558997546412954 1103 72-73 0.501243013523414 1103 74-75 0.550139932267971 1103 76-77 0.3526202870673565 1103 78-79 0.5655257261276816 1103 80-81 0.5938395440164506 1103 82-83 0.7512163772352274 1103 84-85 0.5941455801472024 1103 86-87 0.7515094728177907 1103 88-89 0.8018061469397857 1103 90-91 0.77195040392807 1103 92-93 0.9044388003318424 1103 94-95 0.8170129713714012 1103 96-97 0.6530735892598507 1103 98-99 1.0688746295604403 1103 100-101 1.5601090180359058 1104 1 0.08624778728458438 1104 2 0.029562901466817948 1104 3 0.013480608712377773 1104 4 -0.009359035141400796 1104 5 0.09173432865591735 1104 6 -0.05213587847946144 1104 7 -0.01611078148363987 1104 8 -0.03047153945117742 1104 9 0.05992392726727758 1104 10-11 0.044111131723269636 1104 12-13 0.12000533527134394 1104 14-15 0.010236120013956906 1104 16-17 0.021242893001641505 1104 18-19 0.07535571860546497 1104 20-21 0.02010663944261637 1104 22-23 0.0711925946374663 1104 24-25 -0.039921851575236644 1104 26-27 0.03447374798378178 1104 28-29 -0.03368247770379185 1104 30-31 -0.01666527127658668 1104 32-33 0.06025742629923769 1104 34-35 -0.05042294200200104 1104 36-37 0.015722290520209015 1104 38-39 -0.19159608738409872 1104 40-41 -0.03169911833148831 1104 42-43 0.046574449155414754 1104 44-45 0.01739778342448517 1104 46-47 0.24921650074141866 1104 48-49 0.27609592949006867 1104 50-51 0.35447422098302184 1104 52-53 0.42526812740018016 1104 54-55 0.25401400087257997 1104 56-57 0.2708692532483994 1104 58-59 0.07192333296989517 1104 60-61 0.16877062243340646 1104 62-63 0.22437196028445783 1104 64-65 0.2840292747669082 1104 66-67 0.4533536587386706 1104 68-69 0.5338511553064933 1104 70-71 0.37430450226978706 1104 72-73 0.1849253467491252 1104 74-75 0.23695700492979554 1104 76-77 0.23885104430267745 1104 78-79 0.35414797822025434 1104 80-81 0.3921213400151409 1104 82-83 0.1832349759204952 1104 84-85 0.045102200053595 1104 86-87 0.23424287335940974 1104 88-89 0.3936591189297438 1104 90-91 0.5543262388153174 1104 92-93 0.24601887593017935 1104 94-95 0.222035692604166 1104 96-97 0.3143235096421897 1104 98-99 0.6469092703804691 1104 100-101 1.038656726035665 1105 1 0.14524600920415764 1105 2 0.1446289804842067 1105 3 0.097127628220818 1105 4 0.056647471876338784 1105 5 0.054391487005958084 1105 6 0.03656164607948398 1105 7 0.05040329813905231 1105 8 0.06746159968046328 1105 9 0.171135922374404 1105 10-11 0.15971158065705282 1105 12-13 0.1490693839983166 1105 14-15 0.12599104756664303 1105 16-17 0.29481191657066574 1105 18-19 0.3257915689945037 1105 20-21 0.363511032940643 1105 22-23 0.2596667557239911 1105 24-25 0.19622315025328163 1105 26-27 0.23104463790673435 1105 28-29 0.25868485984144485 1105 30-31 0.21997705712604443 1105 32-33 0.13108744937645156 1105 34-35 0.286676201745081 1105 36-37 0.2081734878871302 1105 38-39 0.290649117473464 1105 40-41 0.1860652923168118 1105 42-43 0.16036765587933388 1105 44-45 0.19704301854724804 1105 46-47 0.14155672571273925 1105 48-49 0.10495568812510925 1105 50-51 -0.011973495886046237 1105 52-53 -0.15278928820405469 1105 54-55 -0.26954950560291735 1105 56-57 -0.2590349700078818 1105 58-59 -0.16815614470321805 1105 60-61 -0.003038763561370672 1105 62-63 0.10775242535968488 1105 64-65 0.00920787096610809 1105 66-67 -0.007288972315947717 1105 68-69 -0.05560930447231982 1105 70-71 -0.17364849839856333 1105 72-73 0.08341555469626627 1105 74-75 0.17935066689274137 1105 76-77 0.20570907529928917 1105 78-79 0.014629983037465877 1105 80-81 0.09850241039434238 1105 82-83 0.15300209152094268 1105 84-85 0.07042171858472202 1105 86-87 0.2635741070445796 1105 88-89 0.2678646574611214 1105 90-91 0.17694364256568207 1105 92-93 0.3166936322322762 1105 94-95 0.5259912910354245 1105 96-97 0.8022592023999806 1105 98-99 0.8792994959823055 1105 100-101 1.3236839647145935 1106 1 -0.4058298518518768 1106 2 -0.33022781616703867 1106 3 -0.30981773419380687 1106 4 -0.15384309139016494 1106 5 -0.17967255059410547 1106 6 -0.3589458078384098 1106 7 -0.28063510973345984 1106 8 -0.4246254973306236 1106 9 -0.5527386461796127 1106 10-11 -0.5314648899311791 1106 12-13 -0.5419326722548945 1106 14-15 -0.6407343691918257 1106 16-17 -0.7019201095731233 1106 18-19 -0.5323223694647723 1106 20-21 -0.5591499790296908 1106 22-23 -0.5744055436077247 1106 24-25 -0.6158362240582349 1106 26-27 -0.7673764533762153 1106 28-29 -0.6760704093074068 1106 30-31 -0.7427166345828482 1106 32-33 -0.9000317398722828 1106 34-35 -1.01147133442295 1106 36-37 -0.8892093791173536 1106 38-39 -0.6643382145379917 1106 40-41 -1.0977435495720016 1106 42-43 -1.1901638488573951 1106 44-45 -0.9248680117824861 1106 46-47 -0.8665902596731812 1106 48-49 -1.149285334126489 1106 50-51 -1.3289531903855618 1106 52-53 -1.2155600894084273 1106 54-55 -1.1568181771164632 1106 56-57 -1.3472151859144716 1106 58-59 -1.3774395771773342 1106 60-61 -1.6529019860039043 1106 62-63 -1.5095687540484093 1106 64-65 -1.8071108850006965 1106 66-67 -1.7389203889275322 1106 68-69 -1.7916010794594683 1106 70-71 -1.667296144723018 1106 72-73 -1.926425085679739 1106 74-75 -1.9394805734685683 1106 76-77 -1.6585873923514427 1106 78-79 -1.5818624528882168 1106 80-81 -1.8907894674054688 1106 82-83 -1.9568835293940872 1106 84-85 -1.7662300769649555 1106 86-87 -1.6605877861771319 1106 88-89 -2.0046623308622955 1106 90-91 -2.5269604846282654 1106 92-93 -2.4682397231323385 1106 94-95 -2.3556364536954284 1106 96-97 -2.468050887928289 1106 98-99 -2.3658490315935197 1106 100-101 -1.5677752406904197 1107 1 0.097679050133614 1107 2 0.0059132565191291064 1107 3 -0.022631404085203144 1107 4 -0.0755661599496591 1107 5 0.04047398312965811 1107 6 0.023607906779133714 1107 7 -0.05674183471708716 1107 8 0.07746563527868489 1107 9 0.03398930271891487 1107 10-11 0.14175316855875764 1107 12-13 0.23598873575380708 1107 14-15 0.15564738451784876 1107 16-17 0.20927316420771547 1107 18-19 0.012354354406873824 1107 20-21 -0.06679790272342245 1107 22-23 0.22592835806888445 1107 24-25 0.16413616187156066 1107 26-27 0.3373268961425424 1107 28-29 0.3040468423821707 1107 30-31 0.48224846172662694 1107 32-33 0.463880932947319 1107 34-35 0.5338911079705184 1107 36-37 0.3430885810244888 1107 38-39 0.5815799069639169 1107 40-41 0.7969168035421461 1107 42-43 0.7141459847168647 1107 44-45 0.8876281271897994 1107 46-47 0.8057830059531668 1107 48-49 0.8186990240780432 1107 50-51 0.9530083970219252 1107 52-53 0.8165003403085151 1107 54-55 1.0320092442112383 1107 56-57 0.8586903331929818 1107 58-59 0.8043314299022555 1107 60-61 0.9375835150648228 1107 62-63 0.6180737333183046 1107 64-65 0.5917610771426922 1107 66-67 0.657809475040736 1107 68-69 0.7051726457836267 1107 70-71 1.0170308627025122 1107 72-73 1.0234417860847245 1107 74-75 0.9928927539342247 1107 76-77 0.842481340119491 1107 78-79 0.9322997967627522 1107 80-81 1.0142446949677364 1107 82-83 0.9513243977591266 1107 84-85 1.0898894975189002 1107 86-87 1.0965999030132245 1107 88-89 1.2774784082310724 1107 90-91 1.122615728969965 1107 92-93 1.244587846458824 1107 94-95 1.6216854139300096 1107 96-97 1.3830786943394067 1107 98-99 1.4356292786972027 1107 100-101 1.9858698783527906 1108 1 0.03421973119973387 1108 2 0.16588442183161334 1108 3 0.18173060928150875 1108 4 0.1059044858016307 1108 5 0.037816743974296685 1108 6 0.11000844052760073 1108 7 -0.0473130452754944 1108 8 0.0828683047879224 1108 9 -0.026256789068952457 1108 10-11 0.031922534003911096 1108 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-0.3698117362921636 2316 68-69 -0.43426013084760484 2316 70-71 -0.6577695512109969 2316 72-73 -0.45989183629435715 2316 74-75 -0.2877446497412812 2316 76-77 -0.504093178763867 2316 78-79 -0.4163500705652545 2316 80-81 -0.46554811704269383 2316 82-83 -0.5023878167359364 2316 84-85 -0.3528014027429762 2316 86-87 -0.29295571805936405 2316 88-89 -0.4589032015224852 2316 90-91 -0.3064322121572012 2316 92-93 0.016357326696766705 2316 94-95 0.27877021417049264 2316 96-97 0.0014805026095778828 2316 98-99 -0.27328142351052875 2316 100-101 0.21074457702259863 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 7.0 5 16.0 6 25.0 7 54.0 8 151.0 9 310.0 10 515.0 11 979.0 12 1510.0 13 2346.0 14 2914.0 15 4263.0 16 6371.0 17 9317.0 18 9071.0 19 5131.0 20 4035.0 21 4110.0 22 4048.0 23 4188.0 24 4549.0 25 5294.0 26 6260.0 27 7462.0 28 9194.0 29 11215.0 30 14234.0 31 18163.0 32 23728.0 33 32413.0 34 46539.0 35 69942.0 36 114140.0 37 185221.0 38 154156.0 39 13523.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.57244600418032 20.01264418238588 15.745232627151445 21.669677186282353 2 15.544622130513282 20.719499613102915 40.69357750838277 23.042300748001033 3 19.582924941965437 24.651792623162237 30.27069899406758 25.494583440804746 4 13.109878772246583 17.51044622130513 37.10497807583183 32.27469693061645 5 13.463639236220828 37.771988315029894 34.09367322065376 14.670699228095518 6 31.52385865359814 36.9295847304617 17.12329120453959 14.423265411400568 7 27.93822543203508 33.87271085891153 20.393732267216922 17.79533144183647 8 25.89166881609492 36.12096982202734 19.20415269538303 18.783208666494712 9 26.291978333763215 17.494067578024243 20.485426876450862 35.72852721176167 10-11 24.852979107557392 27.516314160433325 26.762832086664943 20.86787464534434 12-13 25.592881297348608 24.780619733132482 27.97981856310439 21.64668040641452 14-15 22.587438741294815 26.249290688676812 26.561129739489296 24.602140830539078 16-17 21.494196543719372 29.690095434614395 27.199767861748775 21.615940159917464 18-19 21.18932164044364 27.84240392055713 30.275277276244516 20.692997162754708 20-21 22.08424308001429 26.59901547461371 30.666792193438475 20.64994925193352 22-23 21.915616657424007 26.52149241027096 30.503037181289415 21.059853751015616 24-25 21.72040237296879 26.842726334794943 30.630384317771476 20.806486974464793 26-27 21.47273800043204 26.86587760392317 30.752489868034576 20.90889452761021 28-29 21.152940903363593 27.25270218509113 30.74519822902252 20.849158682522752 30-31 21.5364972917204 26.906435388186743 30.651341243229304 20.905726076863555 32-33 21.534304874903277 26.85143151921589 30.546685581635284 21.067578024245552 34-35 21.81951250967243 26.927521279339693 30.437903017797268 20.81506319319061 36-37 21.755321309807304 26.71162431091706 30.606599333373314 20.926455045902323 38-39 21.469241681712663 27.118583956667525 30.452024761413465 20.960149600206346 40-41 21.666693534606445 27.209076634887268 30.20947320567838 20.91475662482791 42-43 21.574621946515897 27.078987798318156 30.106447064772496 21.239943190393447 44-45 21.819899406757802 27.112522568996646 29.944996131029143 21.122581893216406 46-47 21.96885478462729 27.6513412432293 29.2828862522569 21.09691771988651 48-49 21.99271343822543 27.737490327572868 29.033531080732526 21.236265153469176 50-51 22.147988135156048 27.875161207118904 28.803520763476914 21.17332989424813 52-53 22.040624193964405 28.255610007737943 28.374645344338408 21.329120453959245 54-55 22.012703120969825 28.21717823059066 28.503482073768378 21.26663657467114 56-57 22.168042300748 28.37903017797266 28.48007480010317 20.972852721176167 58-59 22.081248387928813 27.93068093887026 28.635542945576475 21.35252772762445 60-61 22.038044880061904 28.05564869744648 28.965050296621097 20.94125612587052 62-63 21.821962857879804 27.837438741294818 29.165591952540627 21.175006448284755 64-65 21.848916688160948 27.972078927005416 28.930874387412945 21.248129997420687 66-67 21.834923910239876 28.06196801650761 28.781338663915403 21.321769409337115 68-69 21.712277534175907 28.006577250451382 28.70389476399278 21.577250451379935 70-71 21.847046685581635 27.998903791591434 28.683840598400824 21.4702089244261 72-73 21.92590920815063 27.9686613360846 28.510510704152697 21.59491875161207 74-75 21.748839308743875 28.52682486458602 28.092145989166884 21.632189837503223 76-77 21.833827701831314 28.151276760381737 28.266701057518702 21.74819448026825 78-79 22.049393861232915 28.09614392571576 28.12496775857622 21.72949445447511 80-81 21.872323961826154 28.427972659272633 27.99967758576219 21.700025793139023 82-83 21.761935007528397 28.42290567096231 27.97378118964789 21.8413781318614 84-85 21.823222054781006 28.33227903401679 28.06925231155931 21.77524659964289 86-87 21.642313644570542 28.46027856590147 28.065708021666236 21.83169976786175 88-89 21.655661594015992 28.451508898632966 27.876128449832343 22.016701057518702 90-91 21.820372164173232 28.34549846963058 27.888644826603276 21.945484539592908 92-93 21.745133062930027 28.14447016643345 28.111131889319502 21.999264881317025 94-95 21.598916688160948 28.414108847046688 27.922942997162753 22.06403146762961 96-97 21.46833892184679 28.47311065256642 27.902437451637862 22.15611297394893 98-99 21.628911715964122 28.364768924225718 27.703041675533115 22.303277684277045 100-101 21.332923155505767 28.57952709592163 27.724860968419534 22.36268878015307 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 164.0 1 118.0 2 94.0 3 127.0 4 130.0 5 145.0 6 246.0 7 522.0 8 910.0 9 1191.5 10 1247.0 11 1279.5 12 1419.0 13 1509.5 14 1615.5 15 1696.0 16 1693.0 17 1745.0 18 1816.5 19 1848.5 20 1906.5 21 2086.5 22 2406.5 23 2881.0 24 3425.5 25 4112.0 26 5001.0 27 6118.5 28 7183.0 29 8364.5 30 9971.5 31 11318.5 32 12559.0 33 14014.0 34 15831.0 35 17574.0 36 19762.5 37 21927.0 38 23279.0 39 24299.5 40 24667.0 41 25650.0 42 26791.0 43 27940.5 44 29714.5 45 30981.0 46 31612.5 47 32423.0 48 32617.0 49 30894.5 50 29082.0 51 27274.5 52 25359.0 53 24228.0 54 23179.0 55 21041.5 56 17893.5 57 15511.5 58 13800.0 59 11961.0 60 9621.0 61 7436.0 62 5890.0 63 4514.5 64 3377.0 65 2476.5 66 1775.0 67 1384.0 68 989.0 69 594.5 70 405.0 71 258.0 72 170.0 73 144.0 74 103.5 75 63.5 76 44.0 77 22.0 78 21.0 79 15.0 80 4.0 81 3.0 82 3.0 83 4.0 84 3.0 85 2.0 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043848336342532886 2 0.0 3 0.0 4 0.0 5 0.0058034562806293525 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004384833634253288 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.2896569512509673E-4 22-23 0.0012896569512509672 24-25 0.0 26-27 9.672427134382255E-4 28-29 0.0011606912561258704 30-31 0.0 32-33 0.0 34-35 0.0 36-37 4.5137993293783854E-4 38-39 0.0 40-41 3.224142378127418E-4 42-43 0.023084859427392315 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.224142378127418E-4 84-85 4.5137993293783854E-4 86-87 0.0 88-89 0.0 90-91 0.012509672427134383 92-93 0.001934485426876451 94-95 0.0 96-97 0.0 98-99 6.448284756254836E-4 100-101 0.016314160433324735 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 775400.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.20875712340196 #Duplication Level Percentage of deduplicated Percentage of total 1 82.06076709242745 51.869590965053035 2 10.61423797348688 13.418255802322449 3 3.0996203532617943 5.877694502522344 4 1.3116309926606953 3.3162625940246606 5 0.7180678807806026 2.2694089137188533 6 0.46900138262551894 1.7786996690949681 7 0.31148216629788034 1.3781880418455672 8 0.221900223602488 1.12208298714546 9 0.16175545463086455 0.9201925120633005 >10 0.9420701507639949 10.901270267580053 >50 0.0606067896287836 2.6056352025318223 >100 0.0276227024137461 2.85301070561253 >500 4.122791397643977E-4 0.1942747057525382 >1k 8.245582795287954E-4 1.4954331307323145 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4345 0.5603559453185453 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3718 0.47949445447510963 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2408 0.3105493938612329 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1006 0.1297394892958473 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 902 0.11632705700283724 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.2896569512509673E-4 0.0 14-15 0.0 0.0 0.0 1.2896569512509673E-4 0.0 16-17 0.0 0.0 0.0 1.2896569512509673E-4 0.0 18-19 0.0 0.0 0.0 1.2896569512509673E-4 0.0 20-21 0.0 0.0 0.0 1.2896569512509673E-4 0.0 22-23 0.0 0.0 0.0 3.8689708537529015E-4 0.0 24-25 0.0 0.0 0.0 7.093113231880319E-4 0.0 26-27 0.0 0.0 0.0 7.737941707505803E-4 0.0 28-29 0.0 0.0 0.0 0.001612071189063709 0.0 30-31 0.0 0.0 0.0 0.003997936548877998 0.0 32-33 0.0 0.0 0.0 0.0067062161465050295 0.0 34-35 0.0 0.0 0.0 0.010317255610007738 0.0 36-37 0.0 0.0 0.0 0.017410368841888058 0.0 38-39 0.0 0.0 0.0 0.027276244518957958 0.0 40-41 0.0 0.0 0.0 0.0452024761413464 0.0 42-43 0.0 0.0 0.0 0.06390250193448542 0.0 44-45 0.0 0.0 0.0 0.08337632189837503 0.0 46-47 0.0 0.0 0.0 0.12483879288109362 0.0 48-49 0.0 0.0 0.0 0.15211503740005158 0.0 50-51 0.0 0.0 0.0 0.18222852721176167 0.0 52-53 0.0 0.0 0.0 0.2197575444931648 0.0 54-55 0.0 0.0 0.0 0.2695383028114522 0.0 56-57 0.0 0.0 0.0 0.39128191900954346 0.0 58-59 0.0 0.0 0.0 0.4915527469693062 0.0 60-61 0.0 0.0 0.0 0.5728656177456797 0.0 62-63 0.0 0.0 0.0 0.6701702347175651 0.0 64-65 0.0 0.0 0.0 0.7716662367810163 0.0 66-67 0.0 0.0 0.0 0.912625741552747 0.0 68-69 0.0 0.0 0.0 1.0718983750322413 0.0 70-71 0.0 0.0 0.0 1.1810033531080733 0.0 72-73 0.0 0.0 0.0 1.3093242197575445 0.0 74-75 0.0 0.0 0.0 1.5221820995615167 0.0 76-77 0.0 0.0 0.0 1.779146247098272 0.0 78-79 0.0 0.0 0.0 2.0221820995615163 0.0 80-81 0.0 0.0 0.0 2.214598916688161 0.0 82-83 0.0 0.0 0.0 2.500128965695125 0.0 84-85 0.0 0.0 0.0 2.870324993551715 0.0 86-87 0.0 0.0 0.0 3.1922878514315194 0.0 88-89 0.0 0.0 0.0 3.584665978849626 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3125 0.0 37.562103 1 GTATCAA 4730 0.0 34.56211 1 GTACACG 75 3.0688412E-4 31.681934 1 TAGACGG 65 0.0058420417 29.231665 5 GACGGTC 130 3.0356205E-7 29.229776 7 GGACCGA 200 2.3646862E-11 28.499031 6 ATCAACG 5790 0.0 27.811731 3 TATCAAC 5855 0.0 27.665236 2 TCAACGC 5940 0.0 27.109415 4 CAACGCA 6000 0.0 26.996658 5 AACGCAG 6185 0.0 26.264267 6 GTATAGG 230 1.3278623E-10 24.79456 1 AGGACCG 270 1.8189894E-12 24.630383 5 GTACATG 3475 0.0 24.342669 1 TTAGTCG 50 0.0016529908 23.752256 38-39 TACATGG 3440 0.0 23.750725 2 ACGATTC 100 0.0016534888 23.749193 8 ACGGTCC 100 0.0016534888 23.749193 8 ACGCAGA 6850 0.0 23.714523 7 CGCAGAG 6885 0.0 23.387007 8 >>END_MODULE