FastQCFastQC Report
Thu 26 May 2016
SRR937680_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937680_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571068
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25200.4412784466998676No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19900.3484698844971177No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12380.21678679246604607No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7170.12555422471579566No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCGT608.274613E-539.592156
GTATCAA25650.037.8227231
GGTATCA17150.036.8805581
TATCAAC31650.030.7919712
TCAACGC31150.030.5231464
CAACGCA31400.030.2801285
ATCAACG31800.030.048743
AACGCAG32950.028.9821746
CGCAGAG37550.025.0521858
ACGCAGA37650.024.9856437
GAGTACT21250.024.47762312-13
TACATGG21300.024.1046872
GTACATG21650.023.7233431
GCAGAGT39250.023.6039819
ACATGGG21550.022.0602323
GTACTTT23800.022.05461114-15
AGAGTAC33000.021.73587210-11
GTATTAC1100.002855827821.6165961
GTACTAG1100.002855827821.6165961
AGTACTT22650.021.07715412-13
Produced by FastQC (version 0.11.5)