##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937680_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 571068 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.950783444353387 33.0 31.0 34.0 30.0 34.0 2 32.07304208955851 33.0 31.0 34.0 30.0 34.0 3 32.113643208864794 34.0 31.0 34.0 30.0 34.0 4 35.69410998339953 37.0 35.0 37.0 35.0 37.0 5 35.68247213992029 37.0 35.0 37.0 35.0 37.0 6 35.74079269018751 37.0 35.0 37.0 35.0 37.0 7 35.671988274601276 37.0 35.0 37.0 35.0 37.0 8 35.67366583314071 37.0 35.0 37.0 35.0 37.0 9 37.24779185666155 39.0 37.0 39.0 34.0 39.0 10-11 37.200108393396235 39.0 37.0 39.0 34.0 39.0 12-13 37.113631826682635 39.0 37.0 39.0 34.0 39.0 14-15 38.30717270097432 40.0 38.0 41.0 34.0 41.0 16-17 38.21750211883699 40.0 38.0 41.0 34.0 41.0 18-19 38.147052540152835 40.0 38.0 41.0 33.5 41.0 20-21 38.151215792164855 40.0 38.0 41.0 34.0 41.0 22-23 38.15195650955753 40.0 38.0 41.0 34.0 41.0 24-25 38.07842936392864 40.0 38.0 41.0 34.0 41.0 26-27 37.96871738567036 40.0 38.0 41.0 33.0 41.0 28-29 37.81828083520702 40.0 38.0 41.0 33.0 41.0 30-31 37.58542590374526 40.0 37.5 41.0 33.0 41.0 32-33 37.46231359487837 40.0 37.0 41.0 33.0 41.0 34-35 37.48050932638495 40.0 37.0 41.0 33.0 41.0 36-37 37.354899941863316 40.0 37.0 41.0 32.5 41.0 38-39 37.191407152913484 40.0 37.0 41.0 32.0 41.0 40-41 36.971669748611376 40.0 36.5 41.0 31.0 41.0 42-43 36.713665447897625 39.5 36.0 41.0 30.5 41.0 44-45 36.449699510391056 39.0 36.0 41.0 30.0 41.0 46-47 36.07157729027016 39.0 36.0 41.0 29.5 41.0 48-49 35.71120164323688 39.0 35.0 40.5 27.5 41.0 50-51 34.85987132880848 38.5 34.0 39.5 26.5 40.5 52-53 34.97278520246275 38.5 35.0 40.0 27.0 40.5 54-55 35.23815552613699 39.0 35.0 40.0 26.5 41.0 56-57 35.00697990431962 38.5 35.0 40.0 26.0 41.0 58-59 34.75813388247984 38.0 34.0 40.0 25.5 41.0 60-61 34.411897532342905 38.0 34.0 40.0 24.5 41.0 62-63 34.06472258995426 38.0 34.0 40.0 23.0 41.0 64-65 33.61829064139472 37.0 33.5 40.0 22.0 41.0 66-67 33.054194071459094 36.5 32.5 39.0 19.0 41.0 68-69 32.62808457136453 36.0 32.0 39.0 18.5 40.0 70-71 32.175620066261814 35.5 31.5 39.0 16.5 40.0 72-73 31.70342498616627 35.0 31.0 38.0 12.0 40.0 74-75 31.146626146098185 35.0 31.0 37.0 7.5 39.0 76-77 30.593983553622337 34.5 30.0 37.0 6.0 39.0 78-79 30.063644784859246 34.0 29.5 36.0 2.0 39.0 80-81 29.505875832650403 34.0 29.0 36.0 2.0 37.5 82-83 28.965179278124495 34.0 29.0 35.0 2.0 37.0 84-85 28.424028311864788 34.0 27.5 35.0 2.0 36.5 86-87 27.828999348588958 33.5 26.5 35.0 2.0 36.0 88-89 27.289013742671628 33.0 26.0 35.0 2.0 36.0 90-91 26.71830587600776 32.5 25.0 35.0 2.0 35.0 92-93 26.060442889463253 32.0 24.5 34.5 2.0 35.0 94-95 25.294873815377503 32.0 20.5 34.0 2.0 35.0 96-97 24.440465934004358 31.0 12.5 34.0 2.0 35.0 98-99 23.256078085271806 31.0 2.0 34.0 2.0 35.0 100-101 20.05674280470977 27.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.005264748064778502 1101 2 -0.07297427962509317 1101 3 -0.06805368602358186 1101 4 -0.14472501763508916 1101 5 -0.11665944888151358 1101 6 0.0034826221455475093 1101 7 -0.05666124174394582 1101 8 0.09216379206094416 1101 9 0.13570758271558958 1101 10-11 0.11275461417627497 1101 12-13 0.10672539129754455 1101 14-15 0.17950196881277947 1101 16-17 0.19170574790857842 1101 18-19 0.20471502041836231 1101 20-21 0.09007923477482649 1101 22-23 -0.0820071644221585 1101 24-25 0.016582881465417643 1101 26-27 0.09960526683503446 1101 28-29 -0.02373492797523369 1101 30-31 0.09827541466481904 1101 32-33 0.11331974956554802 1101 34-35 0.27376061514542727 1101 36-37 0.22875103742838832 1101 38-39 0.17366235463482127 1101 40-41 0.1323201442350097 1101 42-43 -0.03533425644155841 1101 44-45 -0.013853053160850948 1101 46-47 0.07035736613305943 1101 48-49 0.22211750594944135 1101 50-51 0.18611479256193064 1101 52-53 0.20294348535551165 1101 54-55 0.38377529814115263 1101 56-57 0.5986365731671981 1101 58-59 0.3663953331715035 1101 60-61 0.4826422286581149 1101 62-63 0.41440272469201744 1101 64-65 0.3688741801476141 1101 66-67 0.41277929167944905 1101 68-69 0.6487161697209416 1101 70-71 0.8857795811661937 1101 72-73 0.7650671485206928 1101 74-75 0.7267768051358843 1101 76-77 0.8960075214389143 1101 78-79 1.1215903664685953 1101 80-81 1.16156821823607 1101 82-83 1.0980065669055783 1101 84-85 1.0637565302244916 1101 86-87 1.3212599659688564 1101 88-89 1.5246119764107249 1101 90-91 1.4207663361839842 1101 92-93 1.6399040159360716 1101 94-95 1.7121721508844914 1101 96-97 1.895303456821889 1101 98-99 2.2663490916869513 1101 100-101 2.8040573182531503 1102 1 0.11144793338465675 1102 2 0.17433556480705548 1102 3 0.051349692372603783 1102 4 0.15492097985263342 1102 5 0.18124728067989082 1102 6 0.13741503665350052 1102 7 0.14732501979626988 1102 8 0.2801540591879004 1102 9 0.3233552667661499 1102 10-11 0.33627250316178703 1102 12-13 0.3718188110672216 1102 14-15 0.48211701962927833 1102 16-17 0.32148763668327973 1102 18-19 0.41374779419933816 1102 20-21 0.5452359884927276 1102 22-23 0.4238299241487411 1102 24-25 0.4590772376288399 1102 26-27 0.4285948224432943 1102 28-29 0.41337472496174854 1102 30-31 0.5073064316095 1102 32-33 0.5538244886314345 1102 34-35 0.5516701556448922 1102 36-37 0.7112178112620668 1102 38-39 0.43779829862688047 1102 40-41 0.6264083901218243 1102 42-43 0.6992560932567073 1102 44-45 0.8173105873553084 1102 46-47 0.9105247926314135 1102 48-49 0.9766479475302461 1102 50-51 1.1630080032680965 1102 52-53 1.180249552589629 1102 54-55 1.219251971747326 1102 56-57 1.2928592170301272 1102 58-59 1.0754764199504265 1102 60-61 1.2701265269337796 1102 62-63 1.1405295155670103 1102 64-65 1.243717162904268 1102 66-67 1.2145730215307324 1102 68-69 1.210398691835124 1102 70-71 1.1751524784527483 1102 72-73 1.2555538800146167 1102 74-75 1.3757960512774332 1102 76-77 1.2649080054473103 1102 78-79 1.2260474601343212 1102 80-81 1.4104987441450163 1102 82-83 1.6331608610526764 1102 84-85 1.8431901262048491 1102 86-87 1.5994593844560434 1102 88-89 1.5303612231671622 1102 90-91 1.6882666216698787 1102 92-93 1.9886887244330147 1102 94-95 2.069697119920253 1102 96-97 2.2726323629277765 1102 98-99 2.544899877431984 1102 100-101 3.1919448104571906 1103 1 0.046691774510847495 1103 2 -0.07319729449051238 1103 3 -0.1411662883786704 1103 4 -0.09419199867210892 1103 5 -0.13007930123492173 1103 6 -0.03677156106250834 1103 7 -0.11587974929084766 1103 8 0.015010052867424406 1103 9 -0.2555142297504034 1103 10-11 -0.27320821220128266 1103 12-13 -0.10407000599806082 1103 14-15 -0.11405478242487987 1103 16-17 -0.06469350243903449 1103 18-19 -0.15852111336368324 1103 20-21 -0.14868773484275977 1103 22-23 -0.42405487079486903 1103 24-25 -0.3690991611358001 1103 26-27 -0.2734952443526524 1103 28-29 -0.33240563846566573 1103 30-31 -0.32043902554772075 1103 32-33 0.019744189845255278 1103 34-35 -0.11270533814236217 1103 36-37 -0.17033070558056806 1103 38-39 0.13101815067025768 1103 40-41 0.2514604622327141 1103 42-43 0.25761995162056905 1103 44-45 0.3206719318931235 1103 46-47 0.2943213881927207 1103 48-49 0.14436323877495028 1103 50-51 0.03308629099344529 1103 52-53 0.3034701228690224 1103 54-55 0.361800273119151 1103 56-57 0.46476182422685497 1103 58-59 0.3276921780485438 1103 60-61 0.5224141086331215 1103 62-63 0.483286105382426 1103 64-65 0.19740530482770424 1103 66-67 0.5482870199506138 1103 68-69 0.8860792204568213 1103 70-71 0.8496048234933404 1103 72-73 0.6381797508757856 1103 74-75 0.7494296072639344 1103 76-77 0.49624464266630497 1103 78-79 0.6291574220678129 1103 80-81 0.9791118304051594 1103 82-83 1.1338575499846577 1103 84-85 1.0540211252711416 1103 86-87 1.2003859089119793 1103 88-89 1.5937096394567547 1103 90-91 1.4861190959929438 1103 92-93 1.2889760188379533 1103 94-95 1.4450903541370153 1103 96-97 1.6028557343276155 1103 98-99 1.6392689070951327 1103 100-101 2.31596980507042 1104 1 0.17682003222955345 1104 2 0.14688091810554127 1104 3 0.12346028749575311 1104 4 0.23136383035406283 1104 5 0.17105013240743006 1104 6 0.3149608752872055 1104 7 0.08797618638886462 1104 8 0.2545539038806339 1104 9 0.28938691062206345 1104 10-11 0.26625906703956304 1104 12-13 0.2077193122396963 1104 14-15 0.3900336032780771 1104 16-17 0.5386291655402928 1104 18-19 0.2829780525509378 1104 20-21 0.36199129269572694 1104 22-23 0.40767592473114433 1104 24-25 0.2180367811072088 1104 26-27 0.24586100581826997 1104 28-29 0.237972024454443 1104 30-31 0.24202986716975516 1104 32-33 0.18628779473490908 1104 34-35 0.32763431236218565 1104 36-37 0.29636604285381196 1104 38-39 0.11760988638825864 1104 40-41 0.3051318052469085 1104 42-43 0.555295451388119 1104 44-45 0.6206910748362162 1104 46-47 0.5019977083721159 1104 48-49 0.6932351724856645 1104 50-51 0.6839080701932687 1104 52-53 0.736035003772578 1104 54-55 0.785398052537758 1104 56-57 0.9675211427380788 1104 58-59 0.8018929601744986 1104 60-61 1.0172209016432205 1104 62-63 0.9778221624830863 1104 64-65 1.0763863533140423 1104 66-67 1.1067085475574743 1104 68-69 1.0854529727471416 1104 70-71 1.0925944042628792 1104 72-73 0.9354778611938315 1104 74-75 0.9361717518205026 1104 76-77 1.0819749459452161 1104 78-79 1.5341127096221676 1104 80-81 1.6497383515854622 1104 82-83 1.679040365091172 1104 84-85 1.6668933871466045 1104 86-87 1.706626508359573 1104 88-89 1.4134925037879853 1104 90-91 1.6173029502877974 1104 92-93 1.819191787153649 1104 94-95 2.006391527325956 1104 96-97 2.1219357791767983 1104 98-99 2.378197449223279 1104 100-101 3.0248822124322494 1105 1 0.183646266452552 1105 2 0.20693098870682647 1105 3 0.08622785768555019 1105 4 0.1397380951045335 1105 5 0.2971362226557943 1105 6 0.2034021895971776 1105 7 0.16924995476468752 1105 8 -0.08142156325728678 1105 9 0.19159330891723414 1105 10-11 -0.05322688633760464 1105 12-13 0.16430169050662613 1105 14-15 0.2705918298432124 1105 16-17 0.13998315083096458 1105 18-19 0.2215462759108604 1105 20-21 0.2610877695619038 1105 22-23 0.23096481824016024 1105 24-25 0.06591484446644813 1105 26-27 0.19785213517887001 1105 28-29 0.31256249371473643 1105 30-31 0.18050280045804357 1105 32-33 0.2636243506052125 1105 34-35 0.4063179233361396 1105 36-37 0.5363757451156559 1105 38-39 0.4332062722957204 1105 40-41 0.545371069954065 1105 42-43 0.5852251966171167 1105 44-45 0.7700366052987135 1105 46-47 0.8108804755957948 1105 48-49 0.6908009442919365 1105 50-51 0.7709338938026846 1105 52-53 0.888616065303971 1105 54-55 0.8282396024740848 1105 56-57 0.9120926406113767 1105 58-59 1.0346259660564954 1105 60-61 1.1793742840076291 1105 62-63 1.2666377068925883 1105 64-65 1.251308605278318 1105 66-67 1.4000590331037088 1105 68-69 1.4446185782289191 1105 70-71 1.4265990994645925 1105 72-73 1.4389429547080397 1105 74-75 1.290325965807348 1105 76-77 1.3654282581414776 1105 78-79 1.5211316030445516 1105 80-81 1.9287606754504267 1105 82-83 1.671542768999803 1105 84-85 1.79695134083563 1105 86-87 1.6375758573551273 1105 88-89 1.5866865403620487 1105 90-91 1.6682528190473818 1105 92-93 1.7500891597464907 1105 94-95 1.6194661914284545 1105 96-97 1.810536487788422 1105 98-99 1.4547846786211345 1105 100-101 2.2837853370803245 1106 1 -0.391001514186744 1106 2 -0.07098144880264456 1106 3 -0.13721682241342137 1106 4 -0.06134886141720841 1106 5 -0.40817989106434993 1106 6 -0.14900653635343986 1106 7 -0.24634364216507265 1106 8 -0.12770874196758086 1106 9 0.12687457943179936 1106 10-11 -0.32108402919474344 1106 12-13 -0.5088822967680287 1106 14-15 -0.677919759309944 1106 16-17 -0.7635636602582636 1106 18-19 -0.2155592131564461 1106 20-21 -0.3592727818696986 1106 22-23 -0.2939177753062694 1106 24-25 -0.4748081520492349 1106 26-27 -0.4051089060775013 1106 28-29 0.01190728995773327 1106 30-31 0.10695018216788554 1106 32-33 -0.0716594447793284 1106 34-35 -0.1295573228386857 1106 36-37 -0.3486763934492103 1106 38-39 -0.3727101292595947 1106 40-41 -0.16966579314748742 1106 42-43 -0.07127571233607455 1106 44-45 -0.30364856866482626 1106 46-47 -0.5301505550561245 1106 48-49 -0.7723990759070247 1106 50-51 -0.8913079382429672 1106 52-53 -0.7275816067659164 1106 54-55 -0.6768196057266991 1106 56-57 -0.7012014309939332 1106 58-59 -0.829614931787269 1106 60-61 -0.4993517928494171 1106 62-63 -0.8893645402416794 1106 64-65 -0.22022979799391607 1106 66-67 -0.010614223473581319 1106 68-69 -0.5531942531602638 1106 70-71 -0.41673903112168276 1106 72-73 -0.2679038614309306 1106 74-75 -0.04257182745629606 1106 76-77 0.14120718052697612 1106 78-79 0.001885781704352496 1106 80-81 -0.3610344313515732 1106 82-83 -0.35051197018113456 1106 84-85 0.1627071606609043 1106 86-87 0.4638742717367883 1106 88-89 0.3507437539155518 1106 90-91 0.6622310041708452 1106 92-93 0.5512966788092619 1106 94-95 0.864261803204446 1106 96-97 1.3455406538238712 1106 98-99 1.324084784665672 1106 100-101 1.8668346225421253 1107 1 0.30085314326767687 1107 2 0.3181386710674978 1107 3 0.13841166339361877 1107 4 0.5457330498455875 1107 5 0.14277532411919935 1107 6 0.36387534161210056 1107 7 0.2639368872613801 1107 8 0.38385165094010176 1107 9 0.2074851679608969 1107 10-11 0.2151787694400653 1107 12-13 0.40254178547620256 1107 14-15 0.20758968878794803 1107 16-17 0.527376363049818 1107 18-19 0.7727867453884585 1107 20-21 0.607429956830039 1107 22-23 0.3980201253014073 1107 24-25 0.15343312823046773 1107 26-27 0.3145749780480287 1107 28-29 0.3704875779790413 1107 30-31 0.15630816934844205 1107 32-33 0.41467827269341484 1107 34-35 0.2889944544026406 1107 36-37 0.025444350744578514 1107 38-39 -0.1765528413215307 1107 40-41 -0.1936710374661459 1107 42-43 0.23774888601571575 1107 44-45 -0.11578437986935342 1107 46-47 -0.031144270198055324 1107 48-49 0.11303753443177555 1107 50-51 0.17683698808684056 1107 52-53 0.11108795953725092 1107 54-55 0.27076322121079244 1107 56-57 0.2254145267185379 1107 58-59 0.1591004182501976 1107 60-61 0.18101010675933793 1107 62-63 0.46272798452316266 1107 64-65 0.7764925028468426 1107 66-67 0.5192232442696962 1107 68-69 0.6196445464734666 1107 70-71 0.5067084840701916 1107 72-73 0.7019465090011039 1107 74-75 0.17761671828581882 1107 76-77 0.5759653591667799 1107 78-79 0.4987953796399225 1107 80-81 0.6944211168045911 1107 82-83 0.7415757681023969 1107 84-85 1.2456225439956263 1107 86-87 1.1440696850390388 1107 88-89 1.2064937747263365 1107 90-91 1.1172172690505597 1107 92-93 1.4389860569829018 1107 94-95 1.535092361766008 1107 96-97 1.9883182697399597 1107 98-99 2.111336095745333 1107 100-101 2.3678023241986637 1108 1 0.0383847958053849 1108 2 -0.025938175789267603 1108 3 -0.08125694829855235 1108 4 -0.03312305496559276 1108 5 0.15338580523777523 1108 6 0.35237033225395464 1108 7 0.21805446955798402 1108 8 0.2116893697554758 1108 9 0.5398368138928547 1108 10-11 0.2998837127407441 1108 12-13 0.5053781280942786 1108 14-15 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12 1906.0 13 2474.0 14 2744.0 15 3547.0 16 4512.0 17 6220.0 18 5378.0 19 3685.0 20 3632.0 21 3988.0 22 4293.0 23 4896.0 24 5241.0 25 5887.0 26 6879.0 27 7984.0 28 9460.0 29 11457.0 30 13946.0 31 17413.0 32 22431.0 33 29624.0 34 40295.0 35 57624.0 36 84746.0 37 114497.0 38 79937.0 39 7410.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.87668658755679 19.146182336732707 15.36425725168988 22.612873824020625 2 14.682221352351899 19.72901003881065 42.165111139586685 23.423657469250767 3 18.777692734409563 24.518822281986644 30.348603689321752 26.354881294282045 4 12.362116541802122 16.455613989509974 38.07830305923986 33.10396640944804 5 13.212717181043592 37.27720182697135 34.90712427547807 14.602956716506988 6 31.001487740178806 36.060155230248846 18.35499445606254 14.583362573509802 7 27.798761115662536 32.965866648889794 21.214008410098433 18.021363825349237 8 25.38626854150402 35.42389979035271 20.30982184366804 18.880009824475223 9 25.787956567430488 17.45952100661945 21.192939488093586 35.559582937856476 10-11 24.403845495911593 26.926805218912374 27.711501836514667 20.957847448661365 12-13 24.94960163801256 24.258323844346698 28.698292333308185 22.093782184332557 14-15 22.200408266027335 25.63277286671351 27.03056205441851 25.136256812840642 16-17 21.198767058641906 29.069381897796386 27.80416091393445 21.92769012962726 18-19 21.065917497217367 27.43426713457596 30.494506217398893 21.005309150807776 20-21 21.730538654298368 26.428469308905473 30.736879220502423 21.10411281629374 22-23 21.631090273544235 26.239808426934264 30.67054958838983 21.458551711131676 24-25 21.244312754429405 26.43557174196593 30.76265886350698 21.55745664009768 26-27 21.06829611033481 26.556616062335692 30.723907717489414 21.65118010984008 28-29 20.989955699811393 26.732663713320758 30.845563401903593 21.431817184964252 30-31 21.187181124686827 26.485248398166927 30.73054465833409 21.597025818812153 32-33 21.00754385964912 26.51701754385965 30.73017543859649 21.745263157894737 34-35 21.41621519200539 26.65425696159892 30.529979788906353 21.399548057489334 36-37 21.320639364810376 26.38489424762887 30.647443268784606 21.647023118776143 38-39 21.06202576388688 26.823947942458844 30.59047137896525 21.52355491468903 40-41 21.263079419374645 26.789808358549315 30.441696268034907 21.505415954041133 42-43 21.06904751254058 26.579358459221524 30.44367199690553 21.907922031332365 44-45 21.178516859136177 26.744987693997164 30.284520399195152 21.791975047671507 46-47 21.319820108261354 27.08218020927721 29.821432800681393 21.77656688178004 48-49 21.430432391226734 27.00140267710443 29.74219338697835 21.825971544690486 50-51 21.475267907971407 27.314727504458187 29.440711134814045 21.769293452756358 52-53 21.430996442686013 27.46562213517793 29.237875086081733 21.86550633605432 54-55 21.431108935746934 27.18275427419588 29.392327806329433 21.99380898372775 56-57 21.405574835274606 27.4959196864495 29.34554489397324 21.752960584302656 58-59 21.590927027922906 27.12333187764501 29.505136124396458 21.780604970035622 60-61 21.445329390106675 27.201247519038613 29.673920765350825 21.67950232550389 62-63 21.24776473869258 27.212788106436587 29.69812680936674 21.84132034550409 64-65 21.311791724296405 27.183052515346244 29.668271967345532 21.83688379301181 66-67 21.27911117191926 27.332971992583744 29.263250768726135 22.12466606677086 68-69 21.057953816914736 27.115569974065977 29.567769191572363 22.258707017446923 70-71 21.223305211475264 27.143805633785522 29.50172651661131 22.131162638127908 72-73 21.215704057404835 27.162395306430152 29.373141083383842 22.248759552781173 74-75 21.166514333632154 27.457839420514613 29.13259640400475 22.243049841848485 76-77 21.220770107885272 27.266906411718274 29.111832295412682 22.40049118498377 78-79 21.428107819851174 27.142924804670372 29.121920071851832 22.30704730362662 80-81 21.205407458278845 27.32103121664303 29.226254222109954 22.247307102968172 82-83 21.255237024414157 27.223230330845755 29.175608819162267 22.345923825577817 84-85 21.271673258903906 27.167676696806787 29.148329722950635 22.412320321338676 86-87 21.08074306457568 27.176356366487546 29.32449392517258 22.41840664376419 88-89 21.233854112306176 27.06062618021648 29.049134719874825 22.656384987602518 90-91 21.17396429360772 26.955028468989322 29.206937803239462 22.66406943416349 92-93 21.12434442476013 26.930153829962638 29.363105365631192 22.582396379646035 94-95 20.951954954480872 27.053096879440087 29.209247662649755 22.785700503429283 96-97 20.913255546131587 27.05735103090116 29.168007900929638 22.86138552203761 98-99 20.98554822797611 27.112092669565147 28.967045188429108 22.935313914029628 100-101 21.37430488015568 26.3318105959022 29.530348487333274 22.76353603660884 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 669.0 1 394.5 2 113.5 3 104.0 4 95.5 5 103.5 6 155.5 7 317.0 8 577.0 9 740.5 10 726.0 11 705.5 12 779.5 13 846.5 14 913.0 15 935.5 16 953.0 17 1057.5 18 1129.0 19 1157.5 20 1259.0 21 1466.0 22 1704.5 23 2026.0 24 2475.0 25 3110.5 26 3976.0 27 4796.0 28 5422.5 29 6249.5 30 7377.5 31 8333.0 32 9317.5 33 10610.5 34 12119.0 35 13462.0 36 15041.5 37 16682.0 38 17518.5 39 18165.0 40 18653.5 41 19397.0 42 20137.5 43 20700.5 44 21810.5 45 22880.5 46 23597.5 47 24053.0 48 23818.5 49 22852.0 50 21339.0 51 19543.0 52 18326.5 53 17547.5 54 16725.5 55 15122.5 56 12996.0 57 11315.0 58 9859.5 59 8351.5 60 6879.5 61 5584.0 62 4340.5 63 3237.0 64 2418.5 65 1829.5 66 1300.0 67 932.5 68 711.0 69 498.0 70 329.0 71 210.0 72 144.0 73 99.5 74 76.5 75 59.5 76 40.5 77 27.0 78 17.5 79 11.5 80 7.0 81 5.5 82 6.0 83 3.5 84 3.5 85 4.5 86 4.5 87 3.5 88 2.0 89 1.5 90 1.5 91 1.0 92 0.5 93 0.5 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2887572057968578 2 0.241477372221872 3 0.24935734448436964 4 0.24235292469548284 5 0.24200270370603852 6 0.1884188923210546 7 0.1819398040163343 8 0.18614245588966638 9 0.18929444479466542 10-11 0.1930593204311921 12-13 0.1941975386468862 14-15 0.19200865746285906 16-17 0.183690908963556 18-19 0.17764959689564117 20-21 0.17659893392730813 22-23 0.18395357470563928 24-25 0.1825526907478619 26-27 0.18517934816869444 28-29 0.19139577073133146 30-31 0.19192110221549796 32-33 0.18701800836327723 34-35 0.18981977627883195 36-37 0.18097669629536237 38-39 0.1762487129378638 40-41 0.1738847212591145 42-43 0.17835003887452983 44-45 0.17878781511133524 46-47 0.1853544586634166 48-49 0.19025755251563736 50-51 0.1837784642109171 52-53 0.19533575686258028 54-55 0.1836033537161949 56-57 0.1667927462228666 58-59 0.1560234507974532 60-61 0.17099539809619868 62-63 0.16539186226508928 64-65 0.17117050859092087 66-67 0.16968206938578242 68-69 0.16941940364369917 70-71 0.1732718345275869 72-73 0.17712426541147464 74-75 0.1829904669846673 76-77 0.17826248362716873 78-79 0.17616115769050272 80-81 0.17572338145369729 82-83 0.16959451413842136 84-85 0.1668803014702277 86-87 0.16976962463314352 88-89 0.17379716601175343 90-91 0.17055762185939327 92-93 0.16775585394383857 94-95 0.17125806383828196 96-97 0.17659893392730813 98-99 0.1750229394748086 100-101 0.16329053632842325 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 571068.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.52735943348338 #Duplication Level Percentage of deduplicated Percentage of total 1 85.38648021316216 59.366965005405426 2 8.904835756023749 12.382594326103957 3 2.4184707991346617 5.044496655924583 4 1.024095331871946 2.848105769328529 5 0.5393885715101088 1.8751131542848247 6 0.3612656331375975 1.507070731567361 7 0.24063273337661104 1.1711390981456045 8 0.17119160706953973 0.9522000317375634 9 0.12446138061440028 0.778812403100849 >10 0.7761130616329677 9.706585542309108 >50 0.04118848814390593 1.9424145943052917 >100 0.010612974926471818 1.1927499092319023 >500 5.053797584019834E-4 0.22189470251246896 >1k 7.58069637602975E-4 1.0098580760424953 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2520 0.4412784466998676 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1990 0.3484698844971177 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1238 0.21678679246604607 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 717 0.12555422471579566 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 1.751104947221697E-4 0.0 0.0 0.0 20-21 0.0 1.751104947221697E-4 0.0 0.0 0.0 22-23 0.0 1.751104947221697E-4 0.0 0.0 0.0 24-25 0.0 1.751104947221697E-4 0.0 0.0 0.0 26-27 0.0 1.751104947221697E-4 0.0 5.25331484166509E-4 0.0 28-29 0.0 1.751104947221697E-4 0.0 0.0018386601945827817 0.0 30-31 0.0 1.751104947221697E-4 0.0 0.005165759594304006 0.0 32-33 0.0 1.751104947221697E-4 0.0 0.009280856220274993 0.0 34-35 0.0 1.751104947221697E-4 0.0 0.013308397598884896 0.0 36-37 0.0 1.751104947221697E-4 0.0 0.017948825709022394 0.0 38-39 0.0 1.751104947221697E-4 0.0 0.027054571434575216 0.0 40-41 0.0 1.751104947221697E-4 0.0 0.04552872862776412 0.0 42-43 0.0 1.751104947221697E-4 0.0 0.06242689136845349 0.0 44-45 0.0 1.751104947221697E-4 0.0 0.08055082757219806 0.0 46-47 0.0 1.751104947221697E-4 0.0 0.12485378273690699 0.0 48-49 0.0 1.751104947221697E-4 0.0 0.15812477673411923 0.0 50-51 0.0 1.751104947221697E-4 0.0 0.18990733152619305 0.0 52-53 0.0 1.751104947221697E-4 0.0 0.22834408511770926 0.0 54-55 0.0 1.751104947221697E-4 0.0 0.28359144620255383 0.0 56-57 0.0 1.751104947221697E-4 0.0 0.424117618217095 0.0 58-59 0.0 1.751104947221697E-4 0.0 0.539778099981088 0.0 60-61 0.0 1.751104947221697E-4 0.0 0.624356468931896 0.0 62-63 0.0 1.751104947221697E-4 0.0 0.734763635854224 0.0 64-65 0.0 1.751104947221697E-4 0.0 0.8565529849334931 0.0 66-67 0.0 1.751104947221697E-4 0.0 1.028774156492747 0.0 68-69 0.0 1.751104947221697E-4 0.0 1.2253356868183825 0.0 70-71 0.0 1.751104947221697E-4 0.0 1.3496641380711227 0.0 72-73 0.0 1.751104947221697E-4 0.0 1.4943929619589962 0.0 74-75 0.0 1.751104947221697E-4 0.0 1.7472525163378092 0.0 76-77 0.0 1.751104947221697E-4 0.0 2.043977249644526 0.0 78-79 0.0 1.751104947221697E-4 0.0 2.3154860717112498 0.0 80-81 0.0 1.751104947221697E-4 0.0 2.5251808891410477 0.0 82-83 0.0 1.751104947221697E-4 0.0 2.85465128496081 0.0 84-85 0.0 1.751104947221697E-4 0.0 3.277455574467489 0.0 86-87 0.0 1.751104947221697E-4 0.0 3.6310036633115494 0.0 88-89 0.0 1.751104947221697E-4 0.0 4.022288063768237 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCCGT 60 8.274613E-5 39.59215 6 GTATCAA 2565 0.0 37.822723 1 GGTATCA 1715 0.0 36.880558 1 TATCAAC 3165 0.0 30.791971 2 TCAACGC 3115 0.0 30.523146 4 CAACGCA 3140 0.0 30.280128 5 ATCAACG 3180 0.0 30.04874 3 AACGCAG 3295 0.0 28.982174 6 CGCAGAG 3755 0.0 25.052185 8 ACGCAGA 3765 0.0 24.985643 7 GAGTACT 2125 0.0 24.477623 12-13 TACATGG 2130 0.0 24.104687 2 GTACATG 2165 0.0 23.723343 1 GCAGAGT 3925 0.0 23.603981 9 ACATGGG 2155 0.0 22.060232 3 GTACTTT 2380 0.0 22.054611 14-15 AGAGTAC 3300 0.0 21.735872 10-11 GTATTAC 110 0.0028558278 21.616596 1 GTACTAG 110 0.0028558278 21.616596 1 AGTACTT 2265 0.0 21.077154 12-13 >>END_MODULE