FastQCFastQC Report
Thu 26 May 2016
SRR937680_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937680_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences571068
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32490.5689339973523293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28040.49100982720096376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17960.31449844852101677No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7760.13588574390440367No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6600.115572926516632No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTATA250.00501526357.0135542
GGTATCA23500.037.014371
GTATCAA34200.031.2711281
GCGGTAT700.00836048627.1612071
TAACGCG350.00839256727.14218376-77
TATCAAC41400.025.9361172
GTACATG21800.025.728391
ATCAACG41950.025.4828173
TCAACGC41950.025.4828174
GGTGTGC3950.025.2547478
CAACGCA42500.025.153045
AAGGCCG950.001224100125.0059475
TACATGG22100.024.9380572
ACATGGG22050.024.7791373
AACGCAG43250.024.7103676
GAGTACT28300.024.087212-13
TTAGTCG500.001653879323.74940938-39
GTACTTT28750.023.29507314-15
TGCGTAA1153.6270649E-922.71682560-61
ACGCAGA47550.022.2779467
Produced by FastQC (version 0.11.5)