##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937680_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 571068 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46545069939132 33.0 31.0 34.0 31.0 34.0 2 32.70324549790918 34.0 31.0 34.0 31.0 34.0 3 32.7837858188517 34.0 31.0 34.0 31.0 34.0 4 36.32886276240308 37.0 37.0 37.0 35.0 37.0 5 36.2137591320123 37.0 35.0 37.0 35.0 37.0 6 36.300953651754256 37.0 37.0 37.0 35.0 37.0 7 36.27258400050432 37.0 37.0 37.0 35.0 37.0 8 36.264465877969 37.0 37.0 37.0 35.0 37.0 9 37.993963941246925 39.0 38.0 39.0 35.0 39.0 10-11 37.93936011123019 39.0 38.0 39.0 35.0 39.0 12-13 37.8751225773463 39.0 38.0 39.0 35.0 39.0 14-15 39.17212398523468 40.0 38.5 41.0 36.0 41.0 16-17 39.13668950107518 40.0 38.0 41.0 36.0 41.0 18-19 39.11967664096045 40.0 38.5 41.0 36.0 41.0 20-21 39.046427921018164 40.0 38.0 41.0 36.0 41.0 22-23 38.90568198533275 40.0 38.0 41.0 35.0 41.0 24-25 38.85406291369854 40.0 38.0 41.0 35.0 41.0 26-27 38.78985252194134 40.0 38.0 41.0 35.0 41.0 28-29 38.67406596062115 40.0 38.0 41.0 35.0 41.0 30-31 38.75852525443555 40.0 38.0 41.0 35.0 41.0 32-33 38.679899766752825 40.0 38.0 41.0 35.0 41.0 34-35 38.55045196018688 40.0 38.0 41.0 35.0 41.0 36-37 38.4080556080887 40.0 38.0 41.0 35.0 41.0 38-39 38.28197867854617 40.0 38.0 41.0 34.0 41.0 40-41 38.10864643089789 40.0 38.0 41.0 34.0 41.0 42-43 37.89666817261692 40.0 38.0 41.0 33.5 41.0 44-45 37.58938777868835 40.0 37.0 41.0 33.0 41.0 46-47 37.18950983070317 40.0 37.0 41.0 32.5 41.0 48-49 36.82447361785286 40.0 37.0 41.0 31.5 41.0 50-51 36.546489209691316 40.0 36.5 41.0 31.0 41.0 52-53 36.32658457486674 39.5 36.0 41.0 30.5 41.0 54-55 36.134725636876865 39.0 36.0 41.0 30.0 41.0 56-57 35.85902554511897 39.0 35.0 40.0 29.0 41.0 58-59 35.60985294220653 39.0 35.0 40.0 28.5 41.0 60-61 35.277860079710294 38.0 35.0 40.0 28.0 41.0 62-63 34.92842183417737 38.0 34.5 40.0 27.5 41.0 64-65 34.53601760210693 37.5 34.0 40.0 26.5 41.0 66-67 34.12182699783563 37.0 34.0 39.5 26.0 41.0 68-69 33.66743715284345 36.0 33.5 39.0 26.0 40.5 70-71 33.26433104288806 36.0 33.0 39.0 25.0 40.0 72-73 32.81050155147898 35.0 33.0 38.0 24.0 40.0 74-75 32.32255090462081 35.0 32.0 37.5 22.5 39.0 76-77 31.10815437040773 34.0 30.5 36.0 21.5 39.0 78-79 31.625030644336576 35.0 32.0 36.0 22.0 39.0 80-81 31.417243655746773 35.0 32.0 36.0 20.5 38.0 82-83 31.077553811455026 34.5 31.0 35.5 20.0 37.0 84-85 30.681167566734608 34.0 31.0 35.0 18.0 37.0 86-87 30.27217424194667 34.0 31.0 35.0 16.0 36.0 88-89 29.868126037529684 34.0 30.5 35.0 8.5 36.0 90-91 29.39574008699489 34.0 30.0 35.0 4.5 35.5 92-93 28.957934956957843 34.0 29.0 35.0 2.0 35.0 94-95 28.519082666162348 34.0 29.0 35.0 2.0 35.0 96-97 28.023277438063417 34.0 29.0 35.0 2.0 35.0 98-99 27.28606137973061 33.0 27.0 35.0 2.0 35.0 100-101 24.88410924793545 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.19678052092190512 1101 2 0.09276742799835347 1101 3 0.19951184054814775 1101 4 0.13171717405396066 1101 5 -0.0328000635868122 1101 6 -0.0362018914632074 1101 7 0.08730965069818097 1101 8 -0.010097093841821447 1101 9 0.12077684571351455 1101 10-11 0.08605797687777539 1101 12-13 0.15345599973043278 1101 14-15 0.10068872644570348 1101 16-17 -0.04994149926835689 1101 18-19 0.15436215551614652 1101 20-21 0.0539403095719706 1101 22-23 0.012070293725777503 1101 24-25 -0.07822295064101326 1101 26-27 0.01906617423893664 1101 28-29 0.05019850551736482 1101 30-31 0.058743482933458324 1101 32-33 0.09797102673049807 1101 34-35 0.0031673908578468968 1101 36-37 0.1793002401706545 1101 38-39 0.17910581407728898 1101 40-41 0.0021329472360136492 1101 42-43 0.04028741648030376 1101 44-45 0.0020668065945059766 1101 46-47 0.1155489895269497 1101 48-49 0.09261186351233164 1101 50-51 0.17054684457517055 1101 52-53 0.024435617058053083 1101 54-55 0.1005199011502711 1101 56-57 0.1797944676691543 1101 58-59 0.2262810748754589 1101 60-61 0.17227366006266465 1101 62-63 0.3505936674112178 1101 64-65 0.2689533407725335 1101 66-67 0.40651349016121685 1101 68-69 0.38881216031527543 1101 70-71 0.3762201237692935 1101 72-73 0.447935783636332 1101 74-75 0.20943256502219043 1101 76-77 0.3067104678965542 1101 78-79 0.14433281096060213 1101 80-81 0.1383926293179769 1101 82-83 0.32458387522896004 1101 84-85 0.39639763687673835 1101 86-87 0.5599863386565609 1101 88-89 0.6269948841649295 1101 90-91 0.7275265681731788 1101 92-93 0.5996893805171943 1101 94-95 0.5089885152533391 1101 96-97 0.649779309683904 1101 98-99 0.7469112576482466 1101 100-101 1.2122407513434226 1102 1 0.07757917869898279 1102 2 0.09771292215247485 1102 3 0.007841001395291869 1102 4 -0.009787204860487009 1102 5 -0.0026000477752816664 1102 6 0.024444097240333917 1102 7 -0.07312463923251045 1102 8 0.011406582338118199 1102 9 0.0688799017302415 1102 10-11 0.01206003237623321 1102 12-13 0.15505472075361837 1102 14-15 0.1991110874580002 1102 16-17 0.08614743178461026 1102 18-19 0.21824511508255995 1102 20-21 0.1671728005023141 1102 22-23 0.04037292634496481 1102 24-25 0.11151097650298425 1102 26-27 0.18629063642742238 1102 28-29 -0.10119686170533981 1102 30-31 0.12115509210766362 1102 32-33 0.16684340135826403 1102 34-35 0.11762088300918805 1102 36-37 0.2740288528848822 1102 38-39 0.2978723393186371 1102 40-41 0.2782417305390439 1102 42-43 0.27526427016218946 1102 44-45 0.3356944100062762 1102 46-47 0.33500419459366526 1102 48-49 0.2150545686888492 1102 50-51 0.15764288203092036 1102 52-53 -0.02522136065648084 1102 54-55 -0.05108189433641286 1102 56-57 7.333209821069886E-4 1102 58-59 0.017296614092430218 1102 60-61 0.04832885985887003 1102 62-63 0.0024545962505584384 1102 64-65 0.08964623700499885 1102 66-67 0.29877550472539127 1102 68-69 0.23771106317101243 1102 70-71 0.2239551225834262 1102 72-73 0.16376751385654842 1102 74-75 0.3748826225585802 1102 76-77 0.26861786505575225 1102 78-79 0.39569655513010105 1102 80-81 0.20325063302490065 1102 82-83 0.2780540661092239 1102 84-85 0.4348679595197247 1102 86-87 0.3569048478052821 1102 88-89 0.49517506529474886 1102 90-91 0.593352588675458 1102 92-93 0.6341409401486082 1102 94-95 0.47205542436341474 1102 96-97 0.8774828611284669 1102 98-99 1.1201686956538879 1102 100-101 1.588682455123255 1103 1 0.1419618548463646 1103 2 0.15589003915312105 1103 3 0.09424479368143324 1103 4 0.008369801531813437 1103 5 -0.01057246751241081 1103 6 -0.024597057683173773 1103 7 0.0020172123799255814 1103 8 -8.177945921445939E-4 1103 9 0.030353722027591346 1103 10-11 0.05538402864140579 1103 12-13 0.02713688695342853 1103 14-15 0.1334571335687471 1103 16-17 0.03874385496926891 1103 18-19 0.06420503751306939 1103 20-21 0.03129691168524573 1103 22-23 -0.021675482762269382 1103 24-25 0.13802825007908126 1103 26-27 0.10308299439625301 1103 28-29 4.568659602455227E-5 1103 30-31 -0.02493411280507729 1103 32-33 0.0583610340523677 1103 34-35 0.0016257670140689129 1103 36-37 -0.07542169656566244 1103 38-39 -0.16791807941296355 1103 40-41 0.2150154143715497 1103 42-43 0.33183972085528524 1103 44-45 0.3373535351948149 1103 46-47 0.46653475553599577 1103 48-49 0.350844269184428 1103 50-51 0.42104157484137517 1103 52-53 0.3628774686188265 1103 54-55 0.22307597393909617 1103 56-57 0.4337506304700085 1103 58-59 0.1859456121531906 1103 60-61 0.1062330471748183 1103 62-63 0.43713821328712044 1103 64-65 0.22121270974793816 1103 66-67 0.23952103370621103 1103 68-69 0.34957632209509626 1103 70-71 0.46645314743438604 1103 72-73 0.38238419129675805 1103 74-75 0.5513963049546149 1103 76-77 0.4119634084012951 1103 78-79 0.32230001702768263 1103 80-81 0.4188076250524837 1103 82-83 0.2699667286101217 1103 84-85 0.3238358543699711 1103 86-87 0.41518251784766136 1103 88-89 0.44928696646547195 1103 90-91 0.5037526461343376 1103 92-93 0.49874626945982214 1103 94-95 0.22366786421114782 1103 96-97 0.45927557821529774 1103 98-99 0.6612661602807606 1103 100-101 1.600260419642396 1104 1 0.09529135959108004 1104 2 0.16986438760937972 1104 3 0.08155575711841578 1104 4 0.08228343951140715 1104 5 -0.025175798384509562 1104 6 -0.06462768639212157 1104 7 0.09117410247208824 1104 8 -0.07955545423061494 1104 9 0.21810770786181166 1104 10-11 0.19855136531108286 1104 12-13 0.19324293885814825 1104 14-15 0.005429130177709851 1104 16-17 -0.12793648522818302 1104 18-19 -0.07174267490523079 1104 20-21 0.06116537231915231 1104 22-23 0.21735424349363797 1104 24-25 0.005879044511218012 1104 26-27 -0.08034742100561232 1104 28-29 0.02628792686731174 1104 30-31 0.0440761970371355 1104 32-33 0.04080695523813205 1104 34-35 0.1850288353543732 1104 36-37 -0.06807627583112463 1104 38-39 -0.4951858344926805 1104 40-41 -0.3855205880349857 1104 42-43 -0.22539220957378348 1104 44-45 -0.20419759013676497 1104 46-47 -0.18645017623308746 1104 48-49 -0.14006009814129783 1104 50-51 -0.143844798117847 1104 52-53 -0.1899160337193493 1104 54-55 -0.14670825774521035 1104 56-57 0.06525991479740867 1104 58-59 0.13286770037481688 1104 60-61 0.288534028934329 1104 62-63 0.21802261725791539 1104 64-65 0.21253822182166715 1104 66-67 0.4099479213371353 1104 68-69 0.36824069160859807 1104 70-71 0.45356721202814754 1104 72-73 0.37794849791200846 1104 74-75 0.4791070824315753 1104 76-77 0.23083835028878852 1104 78-79 0.23142846617581725 1104 80-81 0.44799240914743166 1104 82-83 0.6624216743262039 1104 84-85 0.7410863808009047 1104 86-87 0.6297858487197558 1104 88-89 0.9680908059929152 1104 90-91 0.7125611346622449 1104 92-93 0.9826908096189833 1104 94-95 1.1272886151614578 1104 96-97 1.2073626512592988 1104 98-99 1.2453775175154291 1104 100-101 1.718555603824857 1105 1 -0.02360466161529473 1105 2 -0.03771203935944101 1105 3 -0.09095392348658748 1105 4 0.07276309508111467 1105 5 -0.022507676378566543 1105 6 -0.03649978979051127 1105 7 -0.041590955524917206 1105 8 0.11890777679582953 1105 9 0.14399802032662024 1105 10-11 -0.12562112704406303 1105 12-13 0.008401856709447486 1105 14-15 0.17527502014366547 1105 16-17 0.1210521406023588 1105 18-19 0.12080390894570314 1105 20-21 0.08448978303668753 1105 22-23 -0.001990507322823021 1105 24-25 -0.1890411283969442 1105 26-27 -0.13292155234998404 1105 28-29 -0.02315895758047759 1105 30-31 -0.1781229245616558 1105 32-33 -0.41836124906377137 1105 34-35 -0.299616469010779 1105 36-37 -0.12600830802184504 1105 38-39 -0.0800806255908526 1105 40-41 -0.06305465423560719 1105 42-43 -0.23147778005811404 1105 44-45 -0.31979744113774444 1105 46-47 -0.028402929683018385 1105 48-49 -2.1325527462323635E-4 1105 50-51 -0.07355104481518282 1105 52-53 -0.4069435032481792 1105 54-55 -0.08951688929315083 1105 56-57 -0.321106674770931 1105 58-59 -0.2706207825206235 1105 60-61 -0.6452263771746303 1105 62-63 -0.49297992092309073 1105 64-65 -0.37048541877883423 1105 66-67 -0.1820069977962433 1105 68-69 -0.2621031322953513 1105 70-71 -0.5010010806336496 1105 72-73 -0.6327499783131145 1105 74-75 -0.4142026882658634 1105 76-77 -0.8757522575752432 1105 78-79 -0.49648372835453003 1105 80-81 -0.24695375196209213 1105 82-83 -0.05331504786858687 1105 84-85 -0.04341945355029608 1105 86-87 0.31345313044052503 1105 88-89 -0.012496007593714609 1105 90-91 0.3782705692456112 1105 92-93 0.35962801346177287 1105 94-95 0.47667399419937695 1105 96-97 0.6521550488441328 1105 98-99 0.9221276063084467 1105 100-101 1.3316086110928893 1106 1 -0.2528691669932641 1106 2 -0.1244981323756491 1106 3 -0.24060296656087132 1106 4 -0.023560694243734304 1106 5 -0.0546303345616721 1106 6 -0.19848913483923525 1106 7 -0.22153212605463324 1106 8 -0.3693894508969393 1106 9 -0.32808322998899087 1106 10-11 -0.20004798156104897 1106 12-13 -0.35956006825141884 1106 14-15 -0.21377903732237513 1106 16-17 -0.09545230316878417 1106 18-19 -0.17692560173449579 1106 20-21 -0.07695671496867362 1106 22-23 -0.2659625065148603 1106 24-25 -0.032047869799761486 1106 26-27 -0.5255735495221288 1106 28-29 -0.24763883436682477 1106 30-31 -0.3512532583494661 1106 32-33 -0.11158954780639618 1106 34-35 -0.4114839890834858 1106 36-37 -0.3553674960236677 1106 38-39 -0.5146202155519717 1106 40-41 -0.459295122851465 1106 42-43 -0.4336851476286938 1106 44-45 -0.2213464488638479 1106 46-47 -0.08920811071619994 1106 48-49 -0.03197296339966016 1106 50-51 -0.030704001437911188 1106 52-53 0.143604393535135 1106 54-55 -0.23929848804081644 1106 56-57 -0.7387485724631944 1106 58-59 -0.8136319424458804 1106 60-61 -0.44869681094728975 1106 62-63 -0.25781037590465417 1106 64-65 -0.49067554148751213 1106 66-67 -0.4159866978416886 1106 68-69 -0.6261231040168056 1106 70-71 -0.5768355501584708 1106 72-73 -0.38215032169542695 1106 74-75 -0.4262021327944723 1106 76-77 -0.3997574588073789 1106 78-79 -0.1015561921226471 1106 80-81 -0.3301482174471211 1106 82-83 -0.12967658399947624 1106 84-85 -0.3326243719969959 1106 86-87 -0.31128807038763284 1106 88-89 -0.1385059555813939 1106 90-91 -0.07645220003177045 1106 92-93 0.2068165707510694 1106 94-95 -0.31253092424732287 1106 96-97 0.02227573762865731 1106 98-99 0.30094015996193235 1106 100-101 0.550451715673006 1107 1 0.19355345849578498 1107 2 0.24471646847342754 1107 3 0.12679069031084822 1107 4 0.12257266128776934 1107 5 -0.04798128769577659 1107 6 0.13216307526648308 1107 7 0.02625152499006589 1107 8 -0.07410517552859375 1107 9 0.21952144827622533 1107 10-11 0.09543399629627203 1107 12-13 0.1606888287768058 1107 14-15 0.049857231719492745 1107 16-17 0.009689002083852927 1107 18-19 0.09037336605040025 1107 20-21 0.2386492951869954 1107 22-23 0.25572753760400957 1107 24-25 0.397523219853106 1107 26-27 0.10697556393829188 1107 28-29 0.029696136081852842 1107 30-31 -0.0028390404188982643 1107 32-33 0.16069880647646784 1107 34-35 0.030636005921920173 1107 36-37 0.128067116479464 1107 38-39 0.06165652631101182 1107 40-41 -0.05487907357152011 1107 42-43 0.07070072600819799 1107 44-45 0.08437443684329793 1107 46-47 0.43483875517907933 1107 48-49 0.2864373004811398 1107 50-51 0.17906874475371382 1107 52-53 0.0194056088851724 1107 54-55 -0.006436172216290004 1107 56-57 0.1161950303004744 1107 58-59 0.17294509909245193 1107 60-61 0.19376344044703586 1107 62-63 0.038249101328347024 1107 64-65 0.24967865812957513 1107 66-67 0.0777284440878887 1107 68-69 0.4184379964576763 1107 70-71 0.21724169948775085 1107 72-73 0.7773539653269594 1107 74-75 0.4324822091684055 1107 76-77 0.3424905225461572 1107 78-79 0.5358158215292299 1107 80-81 0.18479392856137267 1107 82-83 0.3066684172491705 1107 84-85 0.4699957062515665 1107 86-87 0.7322272879741405 1107 88-89 0.6258847045368121 1107 90-91 0.49506407400469143 1107 92-93 0.6087041092510326 1107 94-95 0.7392359116804208 1107 96-97 1.097402683437366 1107 98-99 1.1360458787665628 1107 100-101 1.5654146724698066 1108 1 -0.015064288944486748 1108 2 -0.042771145750947426 1108 3 -0.026696593626176934 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-0.8926276679759049 2316 86-87 -0.7018286109281746 2316 88-89 -1.0232620531423677 2316 90-91 -0.6654930701702035 2316 92-93 -0.3313014252937556 2316 94-95 -0.7130747607667693 2316 96-97 -0.9383043546587508 2316 98-99 -0.9877711122859196 2316 100-101 -2.3686320021912017 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 2.0 5 8.0 6 12.0 7 35.0 8 102.0 9 160.0 10 315.0 11 536.0 12 920.0 13 1432.0 14 1844.0 15 2814.0 16 4077.0 17 6368.0 18 6184.0 19 3282.0 20 2576.0 21 2533.0 22 2609.0 23 2585.0 24 2846.0 25 3305.0 26 3964.0 27 4939.0 28 6163.0 29 7781.0 30 9776.0 31 12758.0 32 16800.0 33 23349.0 34 33808.0 35 51073.0 36 84553.0 37 140571.0 38 119997.0 39 10986.0 40 4.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73901084461886 20.091452197831074 15.583664745352932 21.58587221219713 2 15.54018085411895 20.8239649218657 40.735078834744726 22.90077538927063 3 19.58768482912718 24.710892573213698 30.317055061743957 25.384367535915164 4 13.16918475558077 17.489861102355587 37.0722926166411 32.268661525422544 5 13.479529398578668 37.92288805528411 34.05328065511529 14.544301891021934 6 31.522165486421933 36.97265474514418 17.118626853544587 14.386552914889295 7 28.01067473575826 33.9057695405801 20.346613713253063 17.73694201040857 8 25.958379737614436 36.042993128664186 19.205768840138127 18.79285829358325 9 26.26937597624101 17.58547143247389 20.456933324928027 35.688219266357066 10-11 24.867791576484763 27.60704504542366 26.69953490652602 20.825628471565558 12-13 25.547774403805 24.88827363502954 27.98797978418249 21.57597217698297 14-15 22.760424327750812 26.238381418675182 26.515581331820375 24.485612921753628 16-17 21.373549209551225 29.791460911835365 27.24062633521752 21.594363543395882 18-19 21.152121988975043 27.971274874445772 30.23212647180371 20.644476664775475 20-21 22.09999509690615 26.705050887109767 30.696607059054266 20.498346956929822 22-23 21.85803880297234 26.74943023073576 30.49200221691245 20.90052874937945 24-25 21.6448783716697 26.919685814274597 30.594145834920283 20.841289979135418 26-27 21.303820576917186 26.990950919573947 30.78578589534237 20.9194426081665 28-29 21.148543875486272 27.408815461248377 30.762441697669306 20.680198965596045 30-31 21.47264939604179 27.047574185820906 30.599703011560837 20.88007340657647 32-33 21.484394152710358 26.97507126997135 30.4962806530921 21.044253924226187 34-35 21.72534619344807 26.94889225101039 30.564223525044305 20.761538030497242 36-37 21.674914370968082 26.82631490470487 30.600996728935957 20.89777399539109 38-39 21.365494126794008 27.226529940392385 30.458281675737393 20.949694257076214 40-41 21.647859799533506 27.32695580911555 30.241669993766067 20.783514397584877 42-43 21.479122992292293 27.267170706163185 30.058652823969723 21.195053477574795 44-45 21.853702186079417 27.259713379142237 29.813699944665085 21.07288449011326 46-47 21.875853663661772 27.781892874403745 29.312095932533428 21.030157529401052 48-49 21.941782764924668 27.8893231629158 29.086115839094468 21.082778233065063 50-51 22.09123957217004 28.052613699244223 28.76942850938942 21.086718219196314 52-53 21.85678575649989 28.424748387887593 28.42772526890429 21.290740586708225 54-55 21.905797558259263 28.350564206013996 28.49870768454895 21.24493055117779 56-57 22.112778163020867 28.37928232714843 28.529702242114773 20.97823726771593 58-59 21.962533358549244 28.104446405681987 28.6866888006332 21.24633143513557 60-61 21.971288883285354 28.19839318610043 29.015371199226713 20.814946731387504 62-63 21.687872547577523 27.99482723598591 29.243977950086503 21.07332226635007 64-65 21.76597182822361 28.145947592931144 28.930004833049654 21.158075745795596 66-67 21.643919813402256 28.215028683099035 28.804363053086497 21.33668845041221 68-69 21.60381951011088 28.089999789867402 28.785888895893308 21.520291804128405 70-71 21.772013140291524 28.05830479032269 28.729941092829574 21.439740976556205 72-73 21.79687883054207 28.048673713112976 28.55658170305463 21.59786575329033 74-75 21.650924233191144 28.65105381495724 28.10427129518726 21.593750656664355 76-77 21.759054963682082 28.284897770493185 28.288750201377066 21.667297064447666 78-79 21.908511770927454 28.290413751076933 28.14839913985725 21.652675338138366 80-81 21.85825505894219 28.44074611079591 28.027835564241038 21.673163266020858 82-83 21.573669616410697 28.61978421077055 27.97765233996391 21.828893832854842 84-85 21.672112603052526 28.38602408119523 28.18701100394349 21.75485231180875 86-87 21.459878683449258 28.674255955507927 27.972325537414104 21.89353982362871 88-89 21.497702550309246 28.667689311955847 27.819278965026932 22.01532917270798 90-91 21.712861169560227 28.472643625018495 27.846559470551718 21.96793573486956 92-93 21.62779803781647 28.290466204650226 27.97788275055928 22.103853006974024 94-95 21.39727668158608 28.66313643909307 27.873738328885526 22.065848550435323 96-97 21.362342137889005 28.614543276807662 27.80553279119124 22.217581794112085 98-99 21.509547949883114 28.668015024559374 27.492492098097415 22.329944927460097 100-101 21.231359849801702 28.792502694935095 27.54604595444487 22.43009150081833 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 146.0 1 101.0 2 68.0 3 85.5 4 88.5 5 101.0 6 165.0 7 374.0 8 672.0 9 864.0 10 913.0 11 959.5 12 1083.5 13 1227.0 14 1284.5 15 1305.5 16 1306.0 17 1318.0 18 1338.5 19 1352.0 20 1398.0 21 1529.5 22 1788.5 23 2129.5 24 2518.5 25 3124.0 26 3849.0 27 4623.0 28 5347.0 29 6151.0 30 7335.5 31 8405.5 32 9380.5 33 10454.5 34 11710.0 35 13042.5 36 14796.0 37 16227.5 38 17350.0 39 18381.0 40 18517.0 41 19100.0 42 19956.5 43 20669.0 44 21799.5 45 22678.0 46 23092.0 47 23793.0 48 23797.0 49 22440.5 50 21363.5 51 20125.5 52 18697.0 53 17867.0 54 16843.5 55 15183.5 56 13029.0 57 11184.5 58 9856.0 59 8575.5 60 6989.0 61 5449.5 62 4227.5 63 3220.5 64 2417.0 65 1720.0 66 1242.0 67 969.5 68 693.5 69 422.5 70 290.5 71 194.0 72 135.5 73 104.0 74 73.0 75 52.5 76 34.5 77 20.5 78 11.0 79 6.5 80 2.5 81 1.5 82 1.0 83 0.5 84 0.5 85 0.5 86 0.5 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04868071753276317 2 0.0 3 0.0 4 0.0 5 0.009455966714997164 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.00525331484166509 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 6.128867315275939E-4 24-25 7.879972262497635E-4 26-27 0.0027142126681936303 28-29 0.0025391021734714604 30-31 3.5022098944433933E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.014183950072495745 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 8.755524736108483E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.626657420832545E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.010243963941246928 92-93 0.0035897651418044787 94-95 0.0 96-97 0.0 98-99 5.25331484166509E-4 100-101 0.015672389277634185 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 571068.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.89224549758322 #Duplication Level Percentage of deduplicated Percentage of total 1 83.51130928414734 55.02747693175639 2 9.79663094796441 12.910440229449849 3 2.8691115362606774 5.671566051217101 4 1.193775965635365 3.1464231598703982 5 0.6721351398575927 2.214424682152447 6 0.40754720927729693 1.6112520459332746 7 0.2736713181988324 1.2622972379083277 8 0.21057446852711625 1.1100179660569485 9 0.15216036211180475 0.902356914174498 >10 0.8419589206517352 10.071967217817056 >50 0.04822480865836336 2.2211530354847713 >100 0.021552977608900382 2.202334972873316 >500 5.388244402222079E-4 0.2549212494796766 >1k 8.082366603333117E-4 1.3933683058258923 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3249 0.5689339973523293 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2804 0.49100982720096376 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1796 0.31449844852101677 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 776 0.13588574390440367 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 660 0.115572926516632 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 7.004419788886788E-4 0.0 28-29 0.0 0.0 0.0 0.0020137706893049515 0.0 30-31 0.0 0.0 0.0 0.0053408700890261755 0.0 32-33 0.0 0.0 0.0 0.009631077209719332 0.0 34-35 0.0 0.0 0.0 0.013833729083051406 0.0 36-37 0.0 0.0 0.0 0.01882437818263324 0.0 38-39 0.0 0.0 0.0 0.02819278965026932 0.0 40-41 0.0 0.0 0.0 0.047367388822346895 0.0 42-43 0.0 0.0 0.0 0.06461577255248062 0.0 44-45 0.0 0.0 0.0 0.08335259548775277 0.0 46-47 0.0 0.0 0.0 0.12791821639454495 0.0 48-49 0.0 0.0 0.0 0.16022610267078524 0.0 50-51 0.0 0.0 0.0 0.19244643369966447 0.0 52-53 0.0 0.0 0.0 0.23149607402270833 0.0 54-55 0.0 0.0 0.0 0.28674343510755285 0.0 56-57 0.0 0.0 0.0 0.4273571623694551 0.0 58-59 0.0 0.0 0.0 0.543893196607059 0.0 60-61 0.0 0.0 0.0 0.6291720075367557 0.0 62-63 0.0 0.0 0.0 0.7415929451483887 0.0 64-65 0.0 0.0 0.0 0.8644329571959907 0.0 66-67 0.0 0.0 0.0 1.0379674574656608 0.0 68-69 0.0 0.0 0.0 1.2344414325439352 0.0 70-71 0.0 0.0 0.0 1.3592952152808422 0.0 72-73 0.0 0.0 0.0 1.506563141342187 0.0 74-75 0.0 0.0 0.0 1.7616115769050271 0.0 76-77 0.0 0.0 0.0 2.0633269593113255 0.0 78-79 0.0 0.0 0.0 2.3420153116616587 0.0 80-81 0.0 0.0 0.0 2.5550372284911775 0.0 82-83 0.0 0.0 0.0 2.8851205110424676 0.0 84-85 0.0 0.0 0.0 3.3116021209383124 0.0 86-87 0.0 0.0 0.0 3.667776867203205 0.0 88-89 0.0 0.0 0.0 4.062475922306976 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGGTATA 25 0.005015263 57.013554 2 GGTATCA 2350 0.0 37.01437 1 GTATCAA 3420 0.0 31.271128 1 GCGGTAT 70 0.008360486 27.161207 1 TAACGCG 35 0.008392567 27.142183 76-77 TATCAAC 4140 0.0 25.936117 2 GTACATG 2180 0.0 25.72839 1 ATCAACG 4195 0.0 25.482817 3 TCAACGC 4195 0.0 25.482817 4 GGTGTGC 395 0.0 25.254747 8 CAACGCA 4250 0.0 25.15304 5 AAGGCCG 95 0.0012241001 25.005947 5 TACATGG 2210 0.0 24.938057 2 ACATGGG 2205 0.0 24.779137 3 AACGCAG 4325 0.0 24.710367 6 GAGTACT 2830 0.0 24.0872 12-13 TTAGTCG 50 0.0016538793 23.749409 38-39 GTACTTT 2875 0.0 23.295073 14-15 TGCGTAA 115 3.6270649E-9 22.716825 60-61 ACGCAGA 4755 0.0 22.277946 7 >>END_MODULE