FastQCFastQC Report
Thu 26 May 2016
SRR937679_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937679_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1209614
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35530.29373006595492446No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29170.24115130942598217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17640.14583164546706637No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14940.12351047524251538No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14890.12309712023835703No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40850.026.6604821
GTATCAA54350.025.2009851
GTACATG34500.023.5722791
TACATGG35150.022.1791572
TCAACGC63250.021.1198254
GGACCGT1353.7944998E-421.1124296
ATCAACG63550.021.0192553
GAGTACT34750.020.91324212-13
TATCAAC64250.020.8642372
CAACGCA63900.020.8314595
AACGCAG64800.020.6725866
ACATGGG36450.020.3448073
CGCGTAT1456.143704E-419.6563997
TGGACCG1951.0251704E-519.5037425
AGAGTAC52500.018.90913610-11
GTATAGG3305.8935257E-1018.7350141
CGCAGAG71300.018.7213638
GTACTTT38350.018.70158214-15
ACGCAGA71400.018.6951437
AGTACTT36600.018.42858312-13
Produced by FastQC (version 0.11.5)