FastQCFastQC Report
Thu 26 May 2016
SRR937679_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937679_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1209614
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50540.41781923820326156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44420.36722458569427935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28520.2357776943719236No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21420.17708128378143773No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44150.030.1346591
GTATCAA63400.030.128381
GTACATG39300.025.2692681
ATCAACG76000.024.7562353
TATCAAC76550.024.6404342
TCAACGC76850.024.4205954
CAACGCA76900.024.4047165
TACATGG39800.024.1141172
AACGCAG79000.023.6889866
ACATGGG40000.022.6869663
GAGTACT49800.021.98643712-13
AGAGTAC69500.021.1880110-11
GTACTTT51150.021.17222814-15
AGTACTT50600.021.16943612-13
ACGCAGA88050.021.0940847
CGCAGAG88300.020.9805648
CATGGGG21900.020.3932674
TCGCGGG1201.4147372E-719.79174486-87
GCAGAGT93450.019.468519
GGGCGTA2451.7553612E-719.3886329
Produced by FastQC (version 0.11.5)