##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937679_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1209614 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.27890798221581 33.0 31.0 34.0 31.0 34.0 2 32.59261880236175 34.0 31.0 34.0 31.0 34.0 3 32.69470260760871 34.0 31.0 34.0 31.0 34.0 4 36.28486773466577 37.0 35.0 37.0 35.0 37.0 5 36.20917003275425 37.0 35.0 37.0 35.0 37.0 6 36.255216953507485 37.0 37.0 37.0 35.0 37.0 7 36.20666179458902 37.0 36.0 37.0 35.0 37.0 8 36.18506316891173 37.0 36.0 37.0 35.0 37.0 9 37.920152213846734 39.0 38.0 39.0 35.0 39.0 10-11 37.84079342666338 39.0 38.0 39.0 35.0 39.0 12-13 37.74466648038134 39.0 37.0 39.0 35.0 39.0 14-15 39.03806462226793 40.0 38.0 41.0 36.0 41.0 16-17 38.99267865616635 40.0 38.0 41.0 36.0 41.0 18-19 38.95166722607377 40.0 38.0 41.0 35.5 41.0 20-21 38.84993766606537 40.0 38.0 41.0 35.0 41.0 22-23 38.79466879516937 40.0 38.0 41.0 35.0 41.0 24-25 38.82673852981199 40.0 38.0 41.0 35.0 41.0 26-27 38.70835820352609 40.0 38.0 41.0 35.0 41.0 28-29 38.56328589120166 40.0 38.0 41.0 34.0 41.0 30-31 38.6370478516287 40.0 38.0 41.0 35.0 41.0 32-33 38.58669707857217 40.0 38.0 41.0 35.0 41.0 34-35 38.4597822115154 40.0 38.0 41.0 34.5 41.0 36-37 38.33168514914675 40.0 38.0 41.0 34.0 41.0 38-39 38.221119299214465 40.0 38.0 41.0 34.0 41.0 40-41 38.05794865138796 40.0 38.0 41.0 34.0 41.0 42-43 37.85741443138059 40.0 38.0 41.0 33.0 41.0 44-45 37.58890935455443 40.0 37.0 41.0 33.0 41.0 46-47 37.25546537986498 40.0 37.0 41.0 32.5 41.0 48-49 36.95247740188192 40.0 37.0 41.0 31.5 41.0 50-51 36.705773908040086 40.0 36.5 41.0 31.0 41.0 52-53 36.48701486589937 39.0 36.0 41.0 31.0 41.0 54-55 36.26792513975533 39.0 36.0 40.5 30.0 41.0 56-57 36.00369456702717 39.0 35.0 40.0 29.5 41.0 58-59 35.751980797179925 38.5 35.0 40.0 29.0 41.0 60-61 35.41851491467526 38.0 35.0 40.0 28.0 41.0 62-63 35.05275277898569 38.0 34.0 40.0 28.0 41.0 64-65 34.64773679868124 37.5 34.0 40.0 27.0 41.0 66-67 34.23241339799308 37.0 34.0 39.0 26.0 41.0 68-69 33.783398670980986 36.0 33.0 39.0 26.0 40.0 70-71 33.381110420348975 36.0 33.0 39.0 26.0 40.0 72-73 32.88409484347899 35.0 32.0 38.0 25.0 40.0 74-75 32.40244780566363 35.0 32.0 37.0 24.0 39.0 76-77 31.18847706789108 34.0 30.5 36.0 22.5 38.5 78-79 31.755984553750203 34.5 31.5 36.0 24.0 39.0 80-81 31.551589598003993 35.0 31.5 36.0 23.0 37.5 82-83 31.17538942174942 34.0 31.0 35.5 21.5 37.0 84-85 30.78232394797018 34.0 31.0 35.0 20.0 37.0 86-87 30.405698016061322 34.0 31.0 35.0 18.0 36.0 88-89 29.979014379793885 34.0 30.0 35.0 15.0 36.0 90-91 29.44247793097633 34.0 29.5 35.0 7.5 35.5 92-93 29.01090347829969 34.0 29.0 35.0 2.0 35.0 94-95 28.536643094408632 34.0 29.0 35.0 2.0 35.0 96-97 27.97347418267315 33.0 29.0 35.0 2.0 35.0 98-99 27.086432118014507 33.0 26.5 35.0 2.0 35.0 100-101 24.29288764845645 30.5 20.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.24069182572227277 1101 2 0.17881481672144872 1101 3 0.20114393971709177 1101 4 0.033346881615266 1101 5 0.09224954128633556 1101 6 0.06723953849266451 1101 7 0.11750516145919221 1101 8 0.06920192369069156 1101 9 0.11996144039204637 1101 10-11 0.1897285799439814 1101 12-13 0.2258968024085064 1101 14-15 0.28928253017200234 1101 16-17 0.278466427691626 1101 18-19 0.3215664671758205 1101 20-21 0.2621517640470543 1101 22-23 0.20822926667572972 1101 24-25 0.1967087142863022 1101 26-27 -0.050451487995580635 1101 28-29 -0.027421213015230705 1101 30-31 0.10885837568910972 1101 32-33 0.19023965189924752 1101 34-35 0.13042789531929344 1101 36-37 0.23815352977037918 1101 38-39 0.16454841436322454 1101 40-41 0.19297016781095522 1101 42-43 0.12564322755679314 1101 44-45 0.17956993728822113 1101 46-47 0.036110351417988795 1101 48-49 0.28766115611943377 1101 50-51 0.38920825024824524 1101 52-53 0.31328174272363185 1101 54-55 0.2599236875712876 1101 56-57 0.11343029847495245 1101 58-59 0.0803042218974852 1101 60-61 0.07181694853586151 1101 62-63 0.02699761793289923 1101 64-65 0.06160253755886913 1101 66-67 0.12598160498297517 1101 68-69 0.013586751592050916 1101 70-71 0.044252899683563385 1101 72-73 0.1581315071499816 1101 74-75 0.058679555859519894 1101 76-77 -0.0043158712791893095 1101 78-79 0.018407679217663286 1101 80-81 0.03436096212864115 1101 82-83 -0.01966744045955693 1101 84-85 0.00953439569287795 1101 86-87 -0.21321059356548133 1101 88-89 -0.33516718380763066 1101 90-91 -0.3028779825588508 1101 92-93 -0.35567902758320713 1101 94-95 -0.6029212039970666 1101 96-97 -0.46657433348300614 1101 98-99 -0.5519629432389834 1101 100-101 -0.4249935111356855 1102 1 0.21735891535858087 1102 2 0.15521504803095354 1102 3 0.08451668026008718 1102 4 0.10976910088854197 1102 5 0.010977377757875217 1102 6 0.055793205106702715 1102 7 0.09428219091434187 1102 8 0.09777320004840107 1102 9 0.11302845137537076 1102 10-11 0.17843722780045113 1102 12-13 0.16517079277834767 1102 14-15 0.203132089879972 1102 16-17 0.22292962594266896 1102 18-19 0.1056974534473838 1102 20-21 0.11387951431027687 1102 22-23 0.18629409339217062 1102 24-25 0.10297145786111628 1102 26-27 0.03472378126010511 1102 28-29 0.06653616520583228 1102 30-31 0.03685336398318384 1102 32-33 0.11499359496107786 1102 34-35 0.0935287987630744 1102 36-37 0.07631097715182733 1102 38-39 0.18303355989544912 1102 40-41 0.16191362193601577 1102 42-43 0.11803461718641728 1102 44-45 0.08058487069956044 1102 46-47 0.017453031411754694 1102 48-49 0.06628255922274917 1102 50-51 0.10582504847138807 1102 52-53 0.10949884432255175 1102 54-55 0.1412417158611774 1102 56-57 0.20324483435940977 1102 58-59 0.1621105863959329 1102 60-61 0.07941732552773573 1102 62-63 0.09664739907032782 1102 64-65 -0.08357129812033293 1102 66-67 -0.05883304229424624 1102 68-69 -0.06171282230639008 1102 70-71 -0.12456453531875411 1102 72-73 -0.1270163013668153 1102 74-75 -0.12549869969098637 1102 76-77 -0.25200253407778206 1102 78-79 -0.09438863765310757 1102 80-81 -0.07719792638463119 1102 82-83 -0.10116795916240662 1102 84-85 -0.02373378094312173 1102 86-87 -0.2561118230510466 1102 88-89 -0.33213826915095623 1102 90-91 -0.281070910958487 1102 92-93 -0.4186418524263118 1102 94-95 -0.5521217429443332 1102 96-97 -0.4373824056810207 1102 98-99 -0.4758511707669477 1102 100-101 -0.22334281560892677 1103 1 0.3795231276683424 1103 2 0.18792657022096648 1103 3 0.19280671488886725 1103 4 0.0431496784286054 1103 5 0.053309816098582985 1103 6 0.11446154975726586 1103 7 0.08672922700219488 1103 8 0.07115709690125982 1103 9 0.10696357815581337 1103 10-11 0.18182628689745428 1103 12-13 0.24754778729334248 1103 14-15 0.14556975858201326 1103 16-17 0.1259306003544296 1103 18-19 0.0824811004354089 1103 20-21 0.11756104966553949 1103 22-23 0.15820160878472223 1103 24-25 0.1277667753900431 1103 26-27 0.06436188452739344 1103 28-29 0.08554228557063226 1103 30-31 0.20827677898586217 1103 32-33 0.13233878618302697 1103 34-35 0.133790739379954 1103 36-37 0.22750618430850977 1103 38-39 0.23915820110930497 1103 40-41 0.10808602663559697 1103 42-43 0.11398678649987914 1103 44-45 0.08420811444199217 1103 46-47 -0.018566094346418538 1103 48-49 -0.09610056482078733 1103 50-51 -0.035527276218495274 1103 52-53 0.037700886616299556 1103 54-55 -0.0765029129103425 1103 56-57 0.0892106576558902 1103 58-59 -0.11255745686916896 1103 60-61 0.03200358947826487 1103 62-63 -0.09725417821416471 1103 64-65 -0.24048061062548953 1103 66-67 -0.13911968430024757 1103 68-69 -0.02578278853289362 1103 70-71 -0.04371050464464332 1103 72-73 -0.05241856440505188 1103 74-75 0.02816511908675068 1103 76-77 0.05100745135185747 1103 78-79 0.06256954816935334 1103 80-81 -7.010028581397876E-4 1103 82-83 -0.07772531250936154 1103 84-85 0.050808209891098244 1103 86-87 0.08657616867306928 1103 88-89 0.1604350230066096 1103 90-91 0.05667298471327342 1103 92-93 0.017599086918849594 1103 94-95 0.017181639191040432 1103 96-97 0.048865610113207225 1103 98-99 0.15263219855954802 1103 100-101 0.24889383102986073 1104 1 0.33390841644222746 1104 2 0.18574080795702486 1104 3 0.15899438579491942 1104 4 0.17370630453662983 1104 5 0.14162305363276317 1104 6 0.16515435013899094 1104 7 0.14292956101456866 1104 8 0.23567654119277393 1104 9 0.24816822496526214 1104 10-11 0.21529818030712988 1104 12-13 0.27208026173772026 1104 14-15 0.24833270156132414 1104 16-17 0.1719114405240063 1104 18-19 0.24341817030272495 1104 20-21 -0.05874609489574567 1104 22-23 0.010102909964778917 1104 24-25 0.1508971319757748 1104 26-27 0.13450815258149618 1104 28-29 0.07579444655725354 1104 30-31 0.1250553948532911 1104 32-33 0.25621057681966874 1104 34-35 0.2257872891422963 1104 36-37 0.23900116494680645 1104 38-39 0.01625357815788675 1104 40-41 0.0692681000816151 1104 42-43 0.15710466350257235 1104 44-45 0.017842425954405883 1104 46-47 -0.03242210198099116 1104 48-49 -0.1403425219130483 1104 50-51 -0.021272105450080403 1104 52-53 -0.08645811795342695 1104 54-55 0.025328649423336458 1104 56-57 0.030648379030850492 1104 58-59 -0.1487225544117905 1104 60-61 -0.0789756907434267 1104 62-63 -0.03764031119514044 1104 64-65 -0.07805241410876107 1104 66-67 0.014720540332241683 1104 68-69 0.05268534264953928 1104 70-71 -0.006379116658074224 1104 72-73 -0.0060018564456427725 1104 74-75 0.02393582510080705 1104 76-77 -0.1497890396830286 1104 78-79 -0.108506265297585 1104 80-81 -0.12599601653062464 1104 82-83 0.005816319746688947 1104 84-85 -0.09037074365916098 1104 86-87 -0.14647729165837475 1104 88-89 -0.16925445560375607 1104 90-91 -0.1310126032035548 1104 92-93 -0.07113258369356856 1104 94-95 -0.14708087250856394 1104 96-97 0.03582628107432129 1104 98-99 0.0028010399770828087 1104 100-101 0.11461168233929797 1105 1 0.2991084223442826 1105 2 0.16495376887066016 1105 3 0.11398088484321534 1105 4 0.0771073639555695 1105 5 0.04401486157988188 1105 6 0.04853858870007599 1105 7 0.07564465404571763 1105 8 0.04743048450352916 1105 9 0.17061964367182014 1105 10-11 0.14284012178824668 1105 12-13 0.10536564087100686 1105 14-15 0.23328393582866624 1105 16-17 0.14504012008666223 1105 18-19 0.16036739787123366 1105 20-21 -0.07480927639870316 1105 22-23 0.03526042106802407 1105 24-25 0.1507023641478824 1105 26-27 0.1005728095982974 1105 28-29 -0.0769647643379443 1105 30-31 -0.12439423848152131 1105 32-33 -0.054046457692606964 1105 34-35 0.006908089461006739 1105 36-37 -0.14204217089490356 1105 38-39 -0.04949236786782052 1105 40-41 0.004272452847466468 1105 42-43 0.010111760724178964 1105 44-45 0.05846553545229227 1105 46-47 -0.10074208191399947 1105 48-49 0.1315452505036987 1105 50-51 0.20434133943209787 1105 52-53 0.3485137735077757 1105 54-55 0.2674803912674335 1105 56-57 0.058557723460040734 1105 58-59 0.028021134077718557 1105 60-61 0.08498783455425496 1105 62-63 0.041903164820389804 1105 64-65 -0.11413020319881184 1105 66-67 -0.10644054151450888 1105 68-69 0.1736029647114279 1105 70-71 -0.09712764483300873 1105 72-73 0.06550302012762188 1105 74-75 0.10017046131287088 1105 76-77 0.12133515431752784 1105 78-79 0.12795958393440898 1105 80-81 0.0448670115188925 1105 82-83 -0.0978525454356074 1105 84-85 0.038373741607831136 1105 86-87 -0.3500620525513547 1105 88-89 -0.12823295736699336 1105 90-91 0.06283081526692769 1105 92-93 0.2628027592896274 1105 94-95 -0.015387572816948136 1105 96-97 0.12181185054888743 1105 98-99 0.2823563201033856 1105 100-101 0.4115190053145277 1106 1 0.19774439056838844 1106 2 0.17172977003642842 1106 3 0.1508702388600227 1106 4 0.07934395215397672 1106 5 0.07549047989827073 1106 6 -0.01428900633836605 1106 7 0.060162959625927215 1106 8 0.007589187326530578 1106 9 0.06406045688027717 1106 10-11 -0.037232826754767245 1106 12-13 -0.19265476882426213 1106 14-15 0.009070936680899422 1106 16-17 -0.014578975475046718 1106 18-19 -3.181064760937602E-4 1106 20-21 0.1305572105460655 1106 22-23 0.15030230465945493 1106 24-25 -0.05644367865861 1106 26-27 0.032908840967941444 1106 28-29 0.05557902907460743 1106 30-31 0.053730250944916236 1106 32-33 0.017420594029331937 1106 34-35 -0.43087700377800786 1106 36-37 -0.1521863016723941 1106 38-39 0.011307284169397747 1106 40-41 -0.1683676485386414 1106 42-43 -0.3260001967431094 1106 44-45 -0.28191426504316297 1106 46-47 -0.34744791647271 1106 48-49 -0.3784708229040845 1106 50-51 -0.1128987151468408 1106 52-53 -0.2009053317686309 1106 54-55 -0.3808519926750549 1106 56-57 -0.1371161453297276 1106 58-59 -0.20811665565207704 1106 60-61 -0.15986999968652071 1106 62-63 -0.21848714521092205 1106 64-65 -0.4543179176397061 1106 66-67 -0.4668028113976277 1106 68-69 -0.12977267171162055 1106 70-71 -0.1743572781915148 1106 72-73 -0.10380317387487281 1106 74-75 -0.11353079409092715 1106 76-77 -0.2826416893774102 1106 78-79 -0.4383531586570264 1106 80-81 -0.47089839621135354 1106 82-83 -0.4194344072131351 1106 84-85 -0.4445604059937409 1106 86-87 -0.4424300541057029 1106 88-89 -0.47567247233866894 1106 90-91 -0.40634047692024566 1106 92-93 -0.5132072380167543 1106 94-95 -0.5562257303805005 1106 96-97 -0.4360744963276133 1106 98-99 -0.5929588555780754 1106 100-101 -1.0231783597946986 1107 1 0.3939097602573156 1107 2 0.2443529829217539 1107 3 0.17637495422870586 1107 4 0.08051776727935334 1107 5 0.08269172258391677 1107 6 0.10987147706232747 1107 7 0.0673164453792765 1107 8 0.0151900801298126 1107 9 0.2544623188979358 1107 10-11 0.2768104762152106 1107 12-13 0.25306947762162224 1107 14-15 0.3199773632558731 1107 16-17 0.2815818698729231 1107 18-19 0.32607287701508625 1107 20-21 0.35424439761248294 1107 22-23 0.3352221735541079 1107 24-25 0.22772891992175204 1107 26-27 0.261832952688998 1107 28-29 0.3208046931473234 1107 30-31 0.40408516035755326 1107 32-33 0.2691750329105105 1107 34-35 0.2251926509645088 1107 36-37 0.37055982247552777 1107 38-39 0.26449066073531924 1107 40-41 0.3288335438765486 1107 42-43 0.4110966342074036 1107 44-45 0.28202975377809736 1107 46-47 0.34337998765148114 1107 48-49 0.3947453299985355 1107 50-51 0.4799709235091285 1107 52-53 0.43668741722093074 1107 54-55 0.23694425111654738 1107 56-57 0.10422225526548345 1107 58-59 0.39725775869712265 1107 60-61 0.23234763116747814 1107 62-63 0.09694864936388825 1107 64-65 0.269938582330127 1107 66-67 0.11275521076158412 1107 68-69 0.2933298699625908 1107 70-71 0.3512907079740586 1107 72-73 0.35688533027746416 1107 74-75 0.33386745850805255 1107 76-77 0.3571038918495901 1107 78-79 0.20045362182194637 1107 80-81 0.14891243606630056 1107 82-83 0.2782151976610905 1107 84-85 0.2729397448176236 1107 86-87 0.17337673642736107 1107 88-89 0.09473129995628327 1107 90-91 0.2364351559847826 1107 92-93 0.1769558393394206 1107 94-95 0.29321174322750565 1107 96-97 0.38109437218498243 1107 98-99 0.3403875480735685 1107 100-101 0.16386166554161363 1108 1 0.17407215646935725 1108 2 0.16932790987238633 1108 3 0.0760960165545228 1108 4 0.001825760760361561 1108 5 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-0.0973330827681913 2316 84-85 -0.20010096061006877 2316 86-87 -0.13862431137225784 2316 88-89 -0.055101777853046485 2316 90-91 0.07650169806459672 2316 92-93 0.09805788867218723 2316 94-95 0.29335802694080115 2316 96-97 -0.006501902910045487 2316 98-99 -0.13479411588665968 2316 100-101 -0.1519291510163434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 1.0 4 3.0 5 14.0 6 37.0 7 79.0 8 199.0 9 396.0 10 698.0 11 1122.0 12 1826.0 13 2750.0 14 3591.0 15 5053.0 16 7329.0 17 10776.0 18 10169.0 19 5831.0 20 4802.0 21 5128.0 22 5262.0 23 5621.0 24 6392.0 25 7758.0 26 9301.0 27 11636.0 28 14681.0 29 18622.0 30 23418.0 31 30434.0 32 39904.0 33 54497.0 34 77678.0 35 115165.0 36 185513.0 37 291390.0 38 232341.0 39 20192.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.76055438969011 19.70329137871444 15.053472707999491 22.48268152359596 2 15.477499433703645 20.358147309802963 40.57550590518959 23.588847351303805 3 19.62791435945682 24.020224633643462 30.390273260726147 25.96158774617357 4 13.109316781483003 17.011143233414323 37.22215287038086 32.657387114721814 5 13.574200740481619 37.45535319253101 34.66188339027119 14.308562676716177 6 32.18952492282662 36.56951721788934 17.084706360872147 14.15625149841189 7 28.579613000510907 33.01309343311172 20.473969381968132 17.93332418440924 8 25.914795959702847 36.26140239778971 19.245726322612004 18.578075319895436 9 25.798891216536845 17.397864112022514 20.547877256711644 36.255367414728994 10-11 24.929522971791002 27.09744596210031 27.19053350903677 20.782497557071924 12-13 25.65255852213776 24.194041758123657 28.237246658978076 21.91615306076051 14-15 22.3389858252302 25.87308843978327 26.437566033461913 25.350359701524617 16-17 21.781989957126818 29.312822106886987 27.01948720831604 21.885700727670148 18-19 21.580686070101702 27.790311620070536 29.58286693110364 21.04613537872412 20-21 22.212581864958576 26.791025897517724 29.903258394826782 21.093133842696925 22-23 22.023337360817223 26.639635815147994 29.827452008113386 21.50957481592139 24-25 22.02213518792175 26.802550403538323 29.923425890522793 21.251888518017132 26-27 21.867550484643484 26.952865842896063 29.848987330941085 21.33059634151937 28-29 21.53796995868886 27.263163638257577 30.00989582515226 21.18897057790131 30-31 21.77335083753991 26.895770055571443 29.895032630244028 21.435846476644617 32-33 21.675303030553547 27.011096101731624 29.796447461752262 21.517153405962564 34-35 22.043726345759886 26.990717700026618 29.68488294612992 21.28067300808357 36-37 22.051621426339313 26.80383990264663 29.762924370915016 21.381614300099038 38-39 21.765455756960485 27.15196748714879 29.799464953282616 21.283111802608104 40-41 21.969385072494678 27.06029897338472 29.66073250298031 21.309583451140288 42-43 21.92326565574011 27.00733246271449 29.448083046950995 21.62131883459441 44-45 21.9670076569881 27.093891109064543 29.335225948112374 21.603875285834988 46-47 22.229074729624493 27.516463929815625 28.79972454022523 21.454736800334654 48-49 22.214276620475623 27.61806658983775 28.601562151231715 21.56609463845491 50-51 21.965147559469383 27.680359188964417 28.64508843316959 21.709404818396614 52-53 22.320972773534685 27.930078492014182 28.141096308862295 21.607852425588835 54-55 22.025350256652334 27.908140868195037 28.282268791754056 21.784240083398572 56-57 22.13123360014021 28.017574201356798 28.28013729999818 21.571054898504812 58-59 22.447078158817607 27.74430520810771 28.185685681548 21.622930951526687 60-61 22.229446749128236 27.75248963719005 28.692376245645306 21.325687368036412 62-63 22.132845684656427 27.747240028637233 28.68592790758043 21.4339863791259 64-65 22.081134973636217 27.879348287966245 28.412493572329684 21.62702316606785 66-67 22.01111263593179 27.9238666219141 28.3206460904057 21.74437465174841 68-69 21.90136688232775 27.860003273771632 28.45647454477213 21.782155299128483 70-71 22.192038121251905 27.846403894134824 28.2718288644146 21.689729120198674 72-73 22.1507852918369 27.718511855848227 28.330029249000095 21.800673603314777 74-75 21.99672788178708 28.196432911656117 27.974750622925992 21.83208858363081 76-77 22.084896504174058 27.933952484015563 28.130048097988286 21.851102913822096 78-79 22.152604053855196 27.88463923201947 27.97156778939397 21.99118892473136 80-81 22.12875347011526 28.114753962834428 27.935399226530116 21.821093340520196 82-83 22.093576480674134 28.216378922288555 27.976590792317683 21.713453804719624 84-85 21.97375442651723 28.116294998361045 27.92007529677868 21.989875278343042 86-87 21.95117616032883 28.100617221692207 28.036671202548913 21.91153541543005 88-89 22.06600618048402 28.06498602033376 27.768982501855966 22.100025297326255 90-91 22.034921312120595 27.994471922226577 27.82704210632496 22.143564659327865 92-93 22.286550456971135 27.76474770481029 27.89743674535692 22.051265092861662 94-95 22.02909357861268 27.73062315747007 27.946807824644885 22.293475439272363 96-97 21.825681581066355 27.924031963915763 28.062464554808393 22.18782190020949 98-99 21.955053681035483 28.053730225359903 27.647066361276075 22.344149732328535 100-101 21.60740737984527 28.371652285431853 27.60812592479588 22.412814409927 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 148.0 1 116.5 2 103.5 3 131.5 4 130.5 5 135.5 6 218.5 7 521.5 8 925.5 9 1179.0 10 1235.5 11 1214.5 12 1298.0 13 1471.0 14 1612.5 15 1749.0 16 1900.5 17 1950.0 18 2054.0 19 2237.5 20 2408.0 21 2678.5 22 3274.5 23 3981.5 24 4752.5 25 5829.5 26 7264.5 27 9178.0 28 10918.0 29 12528.0 30 14646.5 31 16857.0 32 19144.5 33 21950.0 34 24827.0 35 27494.5 36 30583.0 37 33028.0 38 35273.0 39 37256.5 40 38583.0 41 41009.0 42 43231.0 43 44685.0 44 46709.5 45 48590.5 46 48738.0 47 49547.5 48 52490.5 49 51055.0 50 47035.5 51 44040.0 52 40794.0 53 38396.5 54 35771.5 55 32583.0 56 29113.5 57 25922.5 58 23138.5 59 19892.0 60 16081.5 61 12472.5 62 9578.0 63 7279.0 64 5371.5 65 3991.5 66 2823.5 67 2089.5 68 1493.5 69 889.0 70 587.0 71 435.5 72 319.5 73 221.5 74 157.5 75 119.5 76 82.5 77 58.0 78 30.5 79 21.0 80 18.5 81 12.5 82 10.0 83 6.0 84 2.0 85 2.0 86 1.5 87 0.0 88 0.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.017526252176314098 2 0.0 3 0.0 4 8.267100083167027E-5 5 0.033151071333499774 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0023974590241184375 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.027035070691972E-4 24-25 1.240065012475054E-4 26-27 0.0011160585112275486 28-29 0.0010747230108117136 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 6.613680066533622E-4 42-43 0.0107058946077013 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 4.1335500415835136E-5 54-55 8.267100083167027E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 3.720195037425162E-4 84-85 4.1335500415835136E-5 86-87 0.0 88-89 0.0 90-91 0.012565992126413881 92-93 0.0015707490158017351 94-95 0.0 96-97 0.0 98-99 0.0012814005128908893 100-101 0.018642310687541647 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1209614.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.8757280590774 #Duplication Level Percentage of deduplicated Percentage of total 1 79.25208670960443 49.03780565357906 2 12.306092143911986 15.228968219332936 3 3.7409412866620437 6.9442039721542725 4 1.6320859762143733 4.039460321330977 5 0.856769912732045 2.6506631064703745 6 0.49600356625569986 1.841434906918216 7 0.32245186956794997 1.3966360935469297 8 0.22767534033201267 1.127006195531321 9 0.1758632024615822 0.9793497322000201 >10 0.9062608714556503 10.1302570689158 >50 0.05884348066084326 2.463619783706091 >100 0.023308847753488963 2.5079307412527383 >500 9.431288929023344E-4 0.35314192759482693 >1k 5.389307959441911E-4 0.8781862760349165 >5k 1.3473269898604776E-4 0.4213360014315994 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5054 0.41781923820326156 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4442 0.36722458569427935 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2852 0.2357776943719236 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2142 0.17708128378143773 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 8.267100083167027E-5 0.0 26-27 0.0 0.0 0.0 5.373615054058568E-4 0.0 28-29 0.0 0.0 0.0 0.0016534200166334052 0.0 30-31 0.0 0.0 0.0 0.0038442015386726675 0.0 32-33 0.0 0.0 0.0 0.0069030285694444676 0.0 34-35 0.0 0.0 0.0 0.009589836096473752 0.0 36-37 0.0 0.0 0.0 0.012814005128908892 0.0 38-39 0.0 0.0 0.0 0.019717033698353356 0.0 40-41 0.0 0.0 0.0 0.027984133781520384 0.0 42-43 0.0 0.0 0.0 0.03513517535345986 0.0 44-45 0.0 0.0 0.0 0.04034344840585509 0.0 46-47 0.0 0.0 0.0 0.045799734460745326 0.0 48-49 0.0 0.0 0.0 0.05369481504016984 0.0 50-51 0.0 0.0 0.0 0.06307797363456441 0.0 52-53 0.0 0.0 0.0 0.07857878629050258 8.267100083167027E-5 54-55 0.0 0.0 0.0 0.09193015292481735 8.267100083167027E-5 56-57 0.0 0.0 0.0 0.12673464427495051 8.267100083167027E-5 58-59 0.0 0.0 0.0 0.1788587102993186 8.267100083167027E-5 60-61 0.0 0.0 0.0 0.225402483767549 8.267100083167027E-5 62-63 0.0 0.0 0.0 0.2533866175490693 8.267100083167027E-5 64-65 0.0 0.0 0.0 0.2994343650123097 8.267100083167027E-5 66-67 0.0 0.0 0.0 0.38305608235354416 8.267100083167027E-5 68-69 0.0 0.0 0.0 0.5019783170499019 8.267100083167027E-5 70-71 0.0 0.0 0.0 0.6209832227470913 8.267100083167027E-5 72-73 0.0 0.0 0.0 0.7587957811334856 8.267100083167027E-5 74-75 0.0 0.0 0.0 0.9239724407951628 8.267100083167027E-5 76-77 0.0 0.0 0.0 1.0587261721507852 8.267100083167027E-5 78-79 0.0 0.0 0.0 1.2013336485854165 8.267100083167027E-5 80-81 0.0 0.0 0.0 1.3432797570133945 8.267100083167027E-5 82-83 0.0 0.0 0.0 1.4927489265170542 8.267100083167027E-5 84-85 0.0 0.0 0.0 1.6636712207365325 8.267100083167027E-5 86-87 0.0 0.0 0.0 1.923547511850888 8.267100083167027E-5 88-89 0.0 0.0 0.0 2.2970137581079584 8.267100083167027E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4415 0.0 30.134659 1 GTATCAA 6340 0.0 30.12838 1 GTACATG 3930 0.0 25.269268 1 ATCAACG 7600 0.0 24.756235 3 TATCAAC 7655 0.0 24.640434 2 TCAACGC 7685 0.0 24.420595 4 CAACGCA 7690 0.0 24.404716 5 TACATGG 3980 0.0 24.114117 2 AACGCAG 7900 0.0 23.688986 6 ACATGGG 4000 0.0 22.686966 3 GAGTACT 4980 0.0 21.986437 12-13 AGAGTAC 6950 0.0 21.18801 10-11 GTACTTT 5115 0.0 21.172228 14-15 AGTACTT 5060 0.0 21.169436 12-13 ACGCAGA 8805 0.0 21.094084 7 CGCAGAG 8830 0.0 20.980564 8 CATGGGG 2190 0.0 20.393267 4 TCGCGGG 120 1.4147372E-7 19.791744 86-87 GCAGAGT 9345 0.0 19.46851 9 GGGCGTA 245 1.7553612E-7 19.388632 9 >>END_MODULE