FastQCFastQC Report
Thu 26 May 2016
SRR937678_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937678_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193465
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34680.29058246366671836No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29160.24433058363672167No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18440.15450809198426432No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15000.1256844566032519No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14760.12367350529759985No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA55450.029.8341241
GTACATG37350.028.12791
GGTATCA37950.027.5579221
ATCAACG62300.026.0142143
GGACCGA1109.464075E-525.9142556
TACATGG38550.025.890432
TCAACGC62750.025.7573264
CAACGCA62750.025.7562455
TATCAAC63600.025.6319332
AACGCAG64000.025.2394056
ACATGGG39100.025.040033
ACGCAGA70450.022.5249527
GAGTACT34200.022.36275112-13
CGCAGAG70300.022.2350968
GTGTAGG5300.021.5263791
AGAGTAC52850.020.49389310-11
GCAGAGT75250.020.3304889
AGTACTT36850.020.23893512-13
GTACTTT38600.019.87432714-15
TACCTGG7950.019.7283692
Produced by FastQC (version 0.11.5)