##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937678_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1193465 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.685026372788478 33.0 31.0 34.0 30.0 34.0 2 31.834049595086576 33.0 31.0 34.0 30.0 34.0 3 31.87563606808746 33.0 31.0 34.0 30.0 34.0 4 35.45258051136816 37.0 35.0 37.0 33.0 37.0 5 35.42558851746804 37.0 35.0 37.0 33.0 37.0 6 35.49859610461974 37.0 35.0 37.0 33.0 37.0 7 35.43530895334174 37.0 35.0 37.0 33.0 37.0 8 35.420008965491235 37.0 35.0 37.0 33.0 37.0 9 37.039835269572215 39.0 37.0 39.0 33.0 39.0 10-11 36.923233190751304 39.0 37.0 39.0 33.0 39.0 12-13 36.797562140490086 39.0 37.0 39.0 33.0 39.0 14-15 37.95662168559614 40.0 38.0 41.0 33.0 41.0 16-17 37.88451902653199 40.0 38.0 41.0 33.0 41.0 18-19 37.811268449430855 40.0 38.0 41.0 33.0 41.0 20-21 37.580346721520954 40.0 37.0 41.0 32.0 41.0 22-23 37.46216353223597 40.0 37.0 41.0 32.0 41.0 24-25 37.31603189033612 39.5 37.0 41.0 32.0 41.0 26-27 37.43180026226157 40.0 37.0 41.0 32.0 41.0 28-29 37.37598840351414 40.0 37.0 41.0 32.0 41.0 30-31 37.19067547016461 39.5 37.0 41.0 31.5 41.0 32-33 36.98335854004935 39.0 36.0 41.0 31.0 41.0 34-35 36.723899737319485 39.0 36.0 41.0 30.0 41.0 36-37 36.766614437792484 39.0 36.0 41.0 30.0 41.0 38-39 36.736745945628904 39.0 36.0 41.0 30.0 41.0 40-41 36.563414930475545 39.0 36.0 41.0 30.0 41.0 42-43 36.34634949495796 39.0 35.5 41.0 30.0 41.0 44-45 36.143849212167936 39.0 35.0 41.0 29.5 41.0 46-47 35.82970216973267 39.0 35.0 40.0 28.5 41.0 48-49 35.530217476004744 39.0 35.0 40.0 27.0 41.0 50-51 34.665902644819916 38.0 34.0 39.5 25.0 40.5 52-53 34.79871382906076 38.0 34.5 39.5 26.0 40.5 54-55 35.09388168065256 38.0 35.0 40.0 26.0 41.0 56-57 34.831866875023564 38.0 34.0 40.0 25.5 41.0 58-59 34.58871730633072 38.0 34.0 40.0 25.0 41.0 60-61 34.26973895338364 38.0 34.0 40.0 23.5 41.0 62-63 33.90122332871094 37.5 33.0 40.0 22.5 41.0 64-65 33.5240916155899 37.0 33.0 40.0 22.0 41.0 66-67 33.09356495582192 36.5 32.5 39.0 20.5 41.0 68-69 32.6010285178032 36.0 32.0 39.0 19.0 40.0 70-71 32.14363010226525 35.5 31.0 39.0 17.5 40.0 72-73 31.65021638673945 35.0 31.0 38.0 14.0 40.0 74-75 31.146189037801697 35.0 30.5 37.0 9.5 39.0 76-77 30.6570561348678 34.5 30.0 37.0 7.0 39.0 78-79 30.092697314123164 34.0 29.5 36.0 4.0 39.0 80-81 29.507277967933703 34.0 29.0 36.0 2.0 37.5 82-83 28.9657329708035 34.0 29.0 35.0 2.0 37.0 84-85 28.464873289120334 34.0 27.5 35.0 2.0 36.5 86-87 27.920503324353877 33.5 26.5 35.0 2.0 36.0 88-89 27.392600118143392 33.0 26.0 35.0 2.0 36.0 90-91 26.834824649235628 32.5 25.0 35.0 2.0 35.0 92-93 26.24153996975194 32.0 24.5 35.0 2.0 35.0 94-95 25.565293913101765 32.0 21.5 34.0 2.0 35.0 96-97 24.743230844641445 31.5 18.0 34.0 2.0 35.0 98-99 23.72875492787807 31.0 2.0 34.0 2.0 35.0 100-101 21.13466628682031 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06562187797455721 1101 2 0.22375435637031416 1101 3 0.18990612503136362 1101 4 0.15773010075662341 1101 5 0.12396731493382873 1101 6 0.1280241710081782 1101 7 -0.011933932476487996 1101 8 0.14493302508179795 1101 9 0.24095288812286242 1101 10-11 0.14230597919328858 1101 12-13 0.23962419908427535 1101 14-15 0.39279079511295834 1101 16-17 0.345429107029382 1101 18-19 0.3922180582297301 1101 20-21 0.4270472244504262 1101 22-23 0.30787244726302987 1101 24-25 0.3497330307388822 1101 26-27 0.44924105537883463 1101 28-29 0.43135386617282734 1101 30-31 0.3336796535570201 1101 32-33 0.15316937517815887 1101 34-35 0.26645154763405543 1101 36-37 0.16686060928663693 1101 38-39 0.22997647371554564 1101 40-41 0.4424871894751021 1101 42-43 0.356532022026272 1101 44-45 0.5056348685948535 1101 46-47 0.5375194394224891 1101 48-49 0.566632145342858 1101 50-51 0.4246352956799626 1101 52-53 0.4395665814141907 1101 54-55 0.5377855601282988 1101 56-57 0.6346649809609346 1101 58-59 0.5680490110298635 1101 60-61 0.6016261511415664 1101 62-63 0.4747303913882135 1101 64-65 0.6170555533134134 1101 66-67 0.6667040402092965 1101 68-69 0.8212891611536648 1101 70-71 0.9153321110513772 1101 72-73 0.7550561997320813 1101 74-75 0.7946941220956241 1101 76-77 0.9110140453578133 1101 78-79 0.7410503427136881 1101 80-81 0.9325122064415226 1101 82-83 1.099683041701276 1101 84-85 1.2959560092617117 1101 86-87 1.3330908149348986 1101 88-89 1.2954822792296419 1101 90-91 1.1736689171275536 1101 92-93 1.5390127148044037 1101 94-95 1.5775184312933455 1101 96-97 1.6428013243735755 1101 98-99 1.9416007485275273 1101 100-101 2.327324414083609 1102 1 0.10967555303776066 1102 2 0.23287201074022335 1102 3 0.17103548298647908 1102 4 0.08106094344692849 1102 5 0.15129395813678315 1102 6 0.20123884055443142 1102 7 -0.027687127786279575 1102 8 0.10088739221232856 1102 9 0.02190911958012265 1102 10-11 0.07484586481866984 1102 12-13 0.17948906098518336 1102 14-15 0.26497950007119186 1102 16-17 0.3003036268738626 1102 18-19 0.24265043580926715 1102 20-21 0.1172323118836971 1102 22-23 0.38072339031992186 1102 24-25 0.34615681340664395 1102 26-27 0.36920295000270187 1102 28-29 0.3996913838839333 1102 30-31 0.28011644995645923 1102 32-33 0.16254035865071614 1102 34-35 0.5121281373929776 1102 36-37 0.579295741683076 1102 38-39 0.6477179917600395 1102 40-41 0.6362309476361006 1102 42-43 0.7425759645728363 1102 44-45 0.696444688259632 1102 46-47 0.6933910450684024 1102 48-49 0.7998546669946336 1102 50-51 0.6983860871049359 1102 52-53 0.6658059200241766 1102 54-55 0.78526433239238 1102 56-57 0.8774547887232629 1102 58-59 0.7759400846334756 1102 60-61 0.8099833064275543 1102 62-63 0.7613527138860334 1102 64-65 0.7579789141315487 1102 66-67 0.6604194313596707 1102 68-69 0.8061742235830209 1102 70-71 1.0002949634274074 1102 72-73 0.7314563822167628 1102 74-75 0.6513975830171397 1102 76-77 0.7540143392634384 1102 78-79 0.6970928084210435 1102 80-81 0.9034619748994501 1102 82-83 0.964162938410265 1102 84-85 0.9721465829333233 1102 86-87 1.0342458201769418 1102 88-89 1.0718840701530716 1102 90-91 1.172066327248725 1102 92-93 1.2652600956081272 1102 94-95 1.24407262909914 1102 96-97 1.2337651154929503 1102 98-99 1.441222034313462 1102 100-101 1.7980252400900163 1103 1 0.1474665238166537 1103 2 0.11467066876807408 1103 3 0.043839037844659146 1103 4 0.11557115328047018 1103 5 -0.024216451130108396 1103 6 0.13842887416142702 1103 7 0.03850166801539956 1103 8 0.12484082040560907 1103 9 0.09667190103248657 1103 10-11 0.014298890024740274 1103 12-13 0.036098133719022485 1103 14-15 0.16171175998064058 1103 16-17 0.0633459083913408 1103 18-19 0.06494790795041183 1103 20-21 0.16744460126312788 1103 22-23 0.21864135563829734 1103 24-25 0.07892890394608543 1103 26-27 -0.10464491037418355 1103 28-29 0.12598395095369597 1103 30-31 0.13412093967904326 1103 32-33 0.001466204058552023 1103 34-35 0.11758428760419548 1103 36-37 0.1052084788810177 1103 38-39 0.23671800659392517 1103 40-41 0.18053193375982346 1103 42-43 0.28425588559063897 1103 44-45 0.18826421261933746 1103 46-47 0.21966144372083107 1103 48-49 0.3134989570201654 1103 50-51 0.16459284838980892 1103 52-53 0.16349471829817475 1103 54-55 0.20478226408729228 1103 56-57 0.31864791107479107 1103 58-59 0.2664348606335665 1103 60-61 0.1963211333292989 1103 62-63 0.33059316951706563 1103 64-65 0.3376937409294598 1103 66-67 0.5177405305835237 1103 68-69 0.3261966721959695 1103 70-71 0.5583094643743038 1103 72-73 0.5428558933578387 1103 74-75 0.5699797437867922 1103 76-77 0.4789780374499166 1103 78-79 0.4290432665408659 1103 80-81 0.3457482721804013 1103 82-83 0.20431447478872045 1103 84-85 0.3565481757629563 1103 86-87 0.3463899099450245 1103 88-89 0.6387989022252505 1103 90-91 0.5834931986669609 1103 92-93 0.7363982996201983 1103 94-95 0.6430522226177224 1103 96-97 0.6675013261604903 1103 98-99 0.8170826056817582 1103 100-101 1.0917431721145654 1104 1 0.1927927463218957 1104 2 0.19044503264082024 1104 3 0.28499215944512457 1104 4 0.05935475061656348 1104 5 0.10701653462169958 1104 6 0.1504891570025748 1104 7 0.23104325839986473 1104 8 0.2473099758621089 1104 9 0.24239346435335563 1104 10-11 0.25623154942137916 1104 12-13 0.3391640150106383 1104 14-15 0.4250076818676689 1104 16-17 0.3753130606108144 1104 18-19 0.5113617156927148 1104 20-21 0.5266390611851222 1104 22-23 0.4305655244939288 1104 24-25 0.39720001753359924 1104 26-27 0.43233829427438764 1104 28-29 0.36686006865381415 1104 30-31 0.2983935391112382 1104 32-33 0.2957143931853565 1104 34-35 0.4119017277060806 1104 36-37 0.5754040266535796 1104 38-39 0.43939624162270974 1104 40-41 0.5409105588228371 1104 42-43 0.7281726782887716 1104 44-45 0.8060630398633606 1104 46-47 0.7292841453048453 1104 48-49 0.5660599164513016 1104 50-51 0.5890970803938487 1104 52-53 0.590534968769127 1104 54-55 0.6443481851783233 1104 56-57 0.6542368096924278 1104 58-59 0.7413543331587036 1104 60-61 0.6633868554232762 1104 62-63 0.6191041408880196 1104 64-65 0.7683920879272605 1104 66-67 0.7525583819459882 1104 68-69 0.8695204536706669 1104 70-71 0.8535353923639022 1104 72-73 0.8856084206204287 1104 74-75 0.9084676314993878 1104 76-77 0.9912341333930321 1104 78-79 0.8139194903727542 1104 80-81 0.9597871164055078 1104 82-83 1.0946050104887952 1104 84-85 1.0481528880132167 1104 86-87 1.046520186683594 1104 88-89 1.028767593355287 1104 90-91 1.2517441472195863 1104 92-93 1.2620779408948408 1104 94-95 1.157778535334959 1104 96-97 1.2263227329369997 1104 98-99 1.2422169950261264 1104 100-101 1.363150744615826 1105 1 0.2087751124226962 1105 2 0.32059242045500724 1105 3 0.380581052720558 1105 4 0.29523644557642825 1105 5 0.4330607509659572 1105 6 0.34431202843302344 1105 7 0.42714471911838103 1105 8 0.462359324460607 1105 9 0.4131593546020724 1105 10-11 0.5286752920190878 1105 12-13 0.4903352217483743 1105 14-15 0.6226721771959944 1105 16-17 0.6630492759023028 1105 18-19 0.4769259346688983 1105 20-21 0.6873092151527516 1105 22-23 0.6645374286918511 1105 24-25 0.6732649602892309 1105 26-27 0.49509194607067286 1105 28-29 0.6027687408475231 1105 30-31 0.6153257012378361 1105 32-33 0.5741184595098758 1105 34-35 0.6585815539990136 1105 36-37 0.6616719566443692 1105 38-39 0.8884484195408575 1105 40-41 0.8292432341748608 1105 42-43 0.5168399371386272 1105 44-45 0.6118572233085473 1105 46-47 0.699086750910844 1105 48-49 0.7171478885528941 1105 50-51 0.6769224521532848 1105 52-53 0.6992801984046082 1105 54-55 0.7367057322239887 1105 56-57 0.8730685638706106 1105 58-59 1.1451702957853698 1105 60-61 1.0868764593185318 1105 62-63 1.0401822432699106 1105 64-65 1.1429147224844343 1105 66-67 1.1408594741949685 1105 68-69 1.2389318893858956 1105 70-71 1.2727589744141738 1105 72-73 1.2922161979595614 1105 74-75 1.1463844626613842 1105 76-77 1.1552070245629231 1105 78-79 0.9775051595397493 1105 80-81 1.1437458609771625 1105 82-83 1.1928255000268884 1105 84-85 1.2485753120163743 1105 86-87 1.3054953807880842 1105 88-89 1.0926273117392888 1105 90-91 1.3112769804871256 1105 92-93 1.3276783488432535 1105 94-95 1.199493563628156 1105 96-97 1.4474659754689156 1105 98-99 1.6284451171474572 1105 100-101 1.9058415591966913 1106 1 0.10796775615390786 1106 2 0.29658807438473644 1106 3 0.17593434720848222 1106 4 0.17311619788888777 1106 5 0.10310395526330751 1106 6 0.09050601188206286 1106 7 -0.030499524902566577 1106 8 0.2709719793454468 1106 9 0.15926519872045475 1106 10-11 0.08446909085628818 1106 12-13 -0.035576138930586865 1106 14-15 0.03233056517571953 1106 16-17 0.03281179649486177 1106 18-19 0.07135160276502717 1106 20-21 0.12038702455384964 1106 22-23 -0.015828093125037412 1106 24-25 0.1138065684634384 1106 26-27 0.1430658632389381 1106 28-29 0.3612653723225634 1106 30-31 0.39478547124225827 1106 32-33 0.38208329031441224 1106 34-35 0.19984037594474557 1106 36-37 0.3196480853085646 1106 38-39 0.4079708806473761 1106 40-41 0.3543292902122417 1106 42-43 0.5160270521299992 1106 44-45 0.3532836702358537 1106 46-47 0.6047985000438771 1106 48-49 0.8197921485991699 1106 50-51 0.7333051819725895 1106 52-53 0.8097596443126989 1106 54-55 0.7448425949766246 1106 56-57 0.7597674574816295 1106 58-59 0.5815228819535818 1106 60-61 0.43964731949429137 1106 62-63 0.7422548796935686 1106 64-65 0.530348377606451 1106 66-67 0.7745652362636548 1106 68-69 0.700197457996758 1106 70-71 0.5915925865882983 1106 72-73 0.5426116230475344 1106 74-75 0.5900890709060924 1106 76-77 0.9332128962448785 1106 78-79 0.8255437389260436 1106 80-81 0.5364959202708945 1106 82-83 0.27284199009438836 1106 84-85 0.5700226559777057 1106 86-87 0.5359937526142673 1106 88-89 0.7928846785847945 1106 90-91 1.1312115330277805 1106 92-93 1.1064826827342706 1106 94-95 0.9205562194745163 1106 96-97 0.8433113278018816 1106 98-99 1.2733850381746201 1106 100-101 0.9258351650888841 1107 1 0.16687731991036614 1107 2 0.3225451980081715 1107 3 0.2820769692726728 1107 4 0.20307787842190805 1107 5 0.23659839146082362 1107 6 0.38739442513391964 1107 7 0.26654999121347345 1107 8 0.16370969237522104 1107 9 0.2501893537934663 1107 10-11 0.21041298103016715 1107 12-13 0.31089800981338556 1107 14-15 0.2663802308872718 1107 16-17 0.20023392052105038 1107 18-19 0.3974755829263614 1107 20-21 0.5902676933608788 1107 22-23 0.5655714304707757 1107 24-25 0.4270866227781127 1107 26-27 0.36002078895900524 1107 28-29 0.3740511860646336 1107 30-31 0.3094400782088087 1107 32-33 0.4155017780188146 1107 34-35 0.5210300055232295 1107 36-37 0.5756284537738239 1107 38-39 0.5457794214006455 1107 40-41 0.6265424983456356 1107 42-43 0.7035778283005811 1107 44-45 0.5240550077348445 1107 46-47 0.7882635925054089 1107 48-49 0.9293507267513235 1107 50-51 0.8458700546437896 1107 52-53 0.929398658823466 1107 54-55 0.7125385938719191 1107 56-57 0.9855928219768586 1107 58-59 0.8172744843517137 1107 60-61 0.9001385681565708 1107 62-63 0.8314523572830126 1107 64-65 0.8264525651301895 1107 66-67 1.2012945029003959 1107 68-69 1.1190574250857424 1107 70-71 1.1804690091841294 1107 72-73 1.0479401002802433 1107 74-75 1.1826018152933848 1107 76-77 0.8327318891218276 1107 78-79 0.917604819926261 1107 80-81 1.0172711702680175 1107 82-83 1.0852215944717827 1107 84-85 1.0740139794377335 1107 86-87 1.1573824593034878 1107 88-89 1.1249027221055847 1107 90-91 1.304597915232815 1107 92-93 1.2898407568646029 1107 94-95 1.4038711410519475 1107 96-97 1.34496435826183 1107 98-99 1.6146998676932647 1107 100-101 1.4898079617195314 1108 1 0.2715806251097739 1108 2 0.32655911463725573 1108 3 0.33903850525617685 1108 4 0.2863779235220889 1108 5 0.32134808542384263 1108 6 0.15056045825034658 1108 7 0.18719299102018283 1108 8 0.2915880507284143 1108 9 0.32581592424639894 1108 10-11 0.4262850253572097 1108 12-13 0.204761162960736 1108 14-15 0.3190540276787175 1108 16-17 0.32340575223292234 1108 18-19 0.12818881016687556 1108 20-21 0.4766925371209467 1108 22-23 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sequence quality scores pass #Quality Count 2 5232.0 3 1139.0 4 1101.0 5 1209.0 6 1355.0 7 1516.0 8 1776.0 9 2251.0 10 2809.0 11 3457.0 12 4254.0 13 5267.0 14 5962.0 15 7116.0 16 8655.0 17 10967.0 18 10005.0 19 7863.0 20 7943.0 21 8544.0 22 9564.0 23 10424.0 24 11933.0 25 13495.0 26 15600.0 27 18216.0 28 21819.0 29 25845.0 30 31594.0 31 39457.0 32 49860.0 33 65317.0 34 87030.0 35 120156.0 36 171469.0 37 223150.0 38 162089.0 39 18019.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.20176931214797 18.924816838043572 14.649763416681584 23.223650433126874 2 14.823457272334009 19.457709123439212 41.61914481669544 24.099688787531342 3 19.267203219991902 23.60355865549211 30.166257616151025 26.962980508364957 4 12.557698042361597 16.199552275791394 37.770535043029405 33.472214638817604 5 13.608482799868286 37.06705665728091 34.988357402541546 14.336103140309259 6 32.12146143255314 35.836411565196954 17.674283891332816 14.36784311091709 7 28.498216159496327 32.418216159496325 20.959328436516262 18.12423924449108 8 25.632101700378758 35.68310634754345 19.99061501061058 18.694176941467212 9 25.55702004768461 17.04338627892139 20.962087377010647 36.43750629638336 10-11 24.62665077112945 26.571685234781 27.861532520086307 20.94013147400324 12-13 25.254438346711126 23.863685017793685 28.494114526009675 22.387762109485514 14-15 22.13141316247238 25.404317194226728 26.620165152156243 25.84410449114465 16-17 21.683296804948498 28.930900495336648 27.313396753660463 22.07240594605439 18-19 21.641397627863913 27.555788178270333 29.461858036158752 21.34095615770701 20-21 22.289165980838213 26.61414500728776 29.53185020918992 21.56483880268411 22-23 21.97459180084431 26.442348453623826 29.587817696926557 21.99524204860531 24-25 21.950258253146004 26.63100733417109 29.593090752497535 21.825643660185364 26-27 21.783096606469588 26.759754096743716 29.58557654089315 21.87157275589355 28-29 21.500811990512155 27.01428843061479 29.699628570481156 21.7852710083919 30-31 21.70187456951135 26.692877592848085 29.6319319428139 21.973315894826666 32-33 21.521993628977114 26.81739745100319 29.60099464855905 22.059614271460646 34-35 22.04249285720582 26.792351302492246 29.397965389715132 21.767190450586806 36-37 21.969815248503775 26.564050263151266 29.5152896342743 21.95084485407066 38-39 21.628187663721462 26.958173756052606 29.568820298985504 21.84481828124043 40-41 21.819980981364928 26.874061552897572 29.564031782774624 21.74192568296288 42-43 21.72470017942019 26.72286400789029 29.352891183228934 22.199544629460586 44-45 21.7637045374994 26.84475199754226 29.209528102412495 22.18201536254584 46-47 21.928822077016733 27.213759985092402 28.830428967081982 22.026988970808883 48-49 22.073012713612744 27.162403401266033 28.65496953291356 22.109614352207664 50-51 21.733577170833016 27.298243287088436 28.83975855218616 22.128420989892383 52-53 21.998736411703096 27.477965916699727 28.441567437203652 22.08173023439353 54-55 21.882095399096656 27.24221956044934 28.584558900678473 22.291126139775532 56-57 21.87908371934142 27.47117214128106 28.545056564627362 22.104687574750162 58-59 22.138316319935807 27.231801875754357 28.531685879131857 22.098195925177983 60-61 21.965599532363793 27.18811069348553 28.983499709825523 21.862790064325157 62-63 21.823426647525153 27.2672184470277 28.901283407603927 22.008071497843222 64-65 21.776411590675423 27.38634885331207 28.7172202522084 22.12001930380411 66-67 21.74744831053252 27.355353208039013 28.583244266016067 22.3139542154124 68-69 21.61992598091652 27.356342001906082 28.760513978981066 22.26321803819633 70-71 21.90722471500825 27.24098945979564 28.578155136901795 22.273630688294315 72-73 21.841631167667046 27.18580605145512 28.638302997443066 22.33425978343477 74-75 21.601441066967055 27.510336054499994 28.526796937396938 22.361425941136005 76-77 21.766771076733004 27.33055186259501 28.479356350549246 22.42332071012274 78-79 21.868310204643475 27.266729812949077 28.42619292126537 22.43876706114208 80-81 21.712728420303428 27.451940670312663 28.437425510556935 22.39790539882697 82-83 21.842710263942664 27.369212997444198 28.54183459514847 22.246242143464666 84-85 21.75493644584291 27.361624475557043 28.31322697685465 22.570212101745398 86-87 21.67103613454828 27.322629535700678 28.607286661150283 22.39904766860076 88-89 21.861320545701126 27.265334416499943 28.28407610811972 22.589268929679214 90-91 21.71185064424148 27.17207258810718 28.515149798471278 22.60092696918006 92-93 21.995867453003186 26.986480260038253 28.529261437310588 22.488390849647967 94-95 21.707468854420934 26.970441230960063 28.53480134766603 22.787288566952977 96-97 21.59401825610243 26.959014412182373 28.656759760031957 22.790207571683236 98-99 21.626767712588197 27.123384943245604 28.392306397779766 22.857540946386433 100-101 21.851790461363507 26.618800736033098 28.74595318174856 22.78345562085483 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1291.0 1 741.5 2 189.0 3 173.0 4 143.0 5 145.5 6 216.5 7 422.5 8 712.5 9 912.0 10 941.5 11 924.5 12 1014.0 13 1128.5 14 1187.0 15 1257.0 16 1375.5 17 1444.5 18 1546.0 19 1753.5 20 1970.5 21 2246.5 22 2748.5 23 3455.5 24 4349.0 25 5473.0 26 6991.5 27 8862.0 28 10420.0 29 12015.5 30 14104.5 31 16347.0 32 18678.5 33 21530.0 34 24489.0 35 26882.5 36 29887.5 37 32797.5 38 34948.5 39 36536.5 40 38009.0 41 40435.0 42 42706.0 43 44675.0 44 46741.5 45 48522.0 46 48777.5 47 49288.5 48 51741.5 49 50673.5 50 46618.5 51 43411.0 52 40522.5 53 38469.5 54 35941.0 55 32620.0 56 29294.0 57 26023.0 58 22864.0 59 19395.0 60 15778.0 61 12437.5 62 9672.0 63 7326.0 64 5457.5 65 4065.0 66 2996.5 67 2207.0 68 1550.0 69 1044.5 70 712.0 71 502.5 72 374.0 73 283.5 74 201.5 75 159.0 76 105.0 77 64.0 78 49.5 79 33.0 80 23.0 81 21.5 82 16.5 83 11.5 84 10.0 85 4.5 86 3.0 87 4.0 88 2.5 89 0.5 90 2.5 91 3.5 92 2.5 93 2.0 94 1.0 95 0.0 96 1.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28471718902523324 2 0.24801732769708368 3 0.24324131834616014 4 0.2513689132065038 5 0.25212301994612324 6 0.1931351149803304 7 0.18559404758413528 8 0.18442099265583825 9 0.1931351149803304 10-11 0.19732459686710546 12-13 0.1937216424444789 14-15 0.1944757491840984 16-17 0.1804828796822697 18-19 0.1858454164973418 20-21 0.17457571022191687 22-23 0.18400204446716073 24-25 0.18366688591621874 26-27 0.18295467399546697 28-29 0.18907131755015857 30-31 0.18697657660677103 32-33 0.1919201652331656 34-35 0.18680899733130005 36-37 0.17889087656529518 38-39 0.17059570242948055 40-41 0.16774685474647352 42-43 0.1782624542822789 44-45 0.17813676982567564 46-47 0.1781786646445434 48-49 0.18949026573883607 50-51 0.18722794551997754 52-53 0.20214250103689677 54-55 0.17696371489737864 56-57 0.1672441169200605 58-59 0.17185254699551306 60-61 0.16259379202574017 62-63 0.16661569463704423 64-65 0.1667413790936475 66-67 0.1634316884030952 68-69 0.16887801485590276 70-71 0.169841595689861 72-73 0.16753738065213475 74-75 0.1780948750068079 76-77 0.168333382210622 78-79 0.17189444181438082 80-81 0.16933885786344802 82-83 0.15597441064463557 84-85 0.15446619716539656 86-87 0.15610009510123882 88-89 0.15894894278424587 90-91 0.16318031948988868 92-93 0.16322221430875644 94-95 0.16116936818423666 96-97 0.165316955252144 98-99 0.17256475891626483 100-101 0.16276137130121118 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1193465.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.01773945906665 #Duplication Level Percentage of deduplicated Percentage of total 1 81.81004279426418 54.00914090326826 2 11.098893587316974 14.654477302627953 3 3.215830474529665 6.369055752360783 4 1.3047925737695956 3.4455782473298466 5 0.7144863345959502 2.3584386342209482 6 0.4231040665801066 1.675942441893424 7 0.27536183298426214 1.272513602284823 8 0.1858946923491306 0.9817877893062605 9 0.1376191034134555 0.817677190236587 >10 0.7669941309885248 9.200070841065935 >50 0.049813880260413675 2.226532762708159 >100 0.016022099762797743 1.772219331074988 >500 3.814763949630886E-4 0.1844323590921615 >1k 7.629527899261772E-4 1.0321328425298706 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3468 0.29058246366671836 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2916 0.24433058363672167 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1844 0.15450809198426432 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1500 0.1256844566032519 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1476 0.12367350529759985 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 2.5136891320650377E-4 0.0 28-29 0.0 0.0 0.0 0.0010473704716937658 0.0 30-31 0.0 0.0 0.0 0.00398000779243631 0.0 32-33 0.0 0.0 0.0 0.0072059088452531075 0.0 34-35 0.0 0.0 0.0 0.009677703158450394 0.0 36-37 0.0 0.0 0.0 0.013992869501828709 0.0 38-39 0.0 0.0 0.0 0.02153393689802382 0.0 40-41 0.0 0.0 0.0 0.03016426958478045 0.0 42-43 0.0 0.0 0.0 0.038962181547008076 0.0 44-45 0.0 0.0 0.0 0.04302597897717989 0.0 46-47 0.0 0.0 0.0 0.04901693807526823 0.0 48-49 0.0 0.0 0.0 0.05840137750164437 0.0 50-51 0.0 0.0 0.0 0.06816287029783027 0.0 52-53 0.0 0.0 0.0 0.08416669110531101 0.0 54-55 0.0 0.0 0.0 0.09786629687506546 0.0 56-57 0.0 0.0 0.0 0.13523647530509902 0.0 58-59 0.0 0.0 0.0 0.19120795331241386 0.0 60-61 0.0 0.0 0.0 0.24433058363672167 0.0 62-63 0.0 0.0 0.0 0.27642201488941864 0.0 64-65 0.0 0.0 0.0 0.33142991206277517 0.0 66-67 0.0 0.0 0.0 0.42217408973032305 0.0 68-69 0.0 0.0 0.0 0.5509168681109207 0.0 70-71 0.0 0.0 0.0 0.683765338740558 0.0 72-73 0.0 0.0 0.0 0.8339582643814439 0.0 74-75 0.0 0.0 0.0 1.0035065963392307 0.0 76-77 0.0 0.0 0.0 1.137360542621694 0.0 78-79 0.0 0.0 0.0 1.2837829345644822 0.0 80-81 0.0 0.0 0.0 1.4319649088997164 0.0 82-83 0.0 0.0 0.0 1.5940559630990436 0.0 84-85 0.0 0.0 0.0 1.778099902385072 0.0 86-87 0.0 0.0 0.0 2.0512122265839383 0.0 88-89 0.0 0.0 0.0 2.4292291772276524 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5545 0.0 29.834124 1 GTACATG 3735 0.0 28.1279 1 GGTATCA 3795 0.0 27.557922 1 ATCAACG 6230 0.0 26.014214 3 GGACCGA 110 9.464075E-5 25.914255 6 TACATGG 3855 0.0 25.89043 2 TCAACGC 6275 0.0 25.757326 4 CAACGCA 6275 0.0 25.756245 5 TATCAAC 6360 0.0 25.631933 2 AACGCAG 6400 0.0 25.239405 6 ACATGGG 3910 0.0 25.04003 3 ACGCAGA 7045 0.0 22.524952 7 GAGTACT 3420 0.0 22.362751 12-13 CGCAGAG 7030 0.0 22.235096 8 GTGTAGG 530 0.0 21.526379 1 AGAGTAC 5285 0.0 20.493893 10-11 GCAGAGT 7525 0.0 20.330488 9 AGTACTT 3685 0.0 20.238935 12-13 GTACTTT 3860 0.0 19.874327 14-15 TACCTGG 795 0.0 19.728369 2 >>END_MODULE