FastQCFastQC Report
Thu 26 May 2016
SRR937678_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937678_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1193465
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46650.3908786600361133No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41700.34940278935704017No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26880.22522654623302737No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT21480.1799801418558567No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGAA300.003952922831.66482548-49
ATACCGT953.4923418E-529.9982556
ACCGCGC700.0083906527.1435538
GTACATG35100.023.8252621
GGTATCA55650.023.6508481
GTATCAA71550.023.4421351
ACCGCTC1652.4294714E-623.0308918
AGTACTT48100.021.97301512-13
TACATGG37900.021.9341982
GAGTACT45400.021.65804312-13
TAGTACC2657.930794E-1021.5108854
ACATGGG36900.021.498743
GACGTCG704.8557497E-420.35595746-47
GTACTTT48200.020.3489214-15
CATGGGG18550.019.9743944
AGAGTAC69250.019.89220410-11
ACCGTCC1200.00476289819.7921738
TCAACGC84100.019.7129824
ATCAACG84900.019.527233
TACACCG1951.0320995E-519.4884955
Produced by FastQC (version 0.11.5)