##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937678_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1193465 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.41172803559384 33.0 31.0 34.0 31.0 34.0 2 32.6455597776223 34.0 31.0 34.0 31.0 34.0 3 32.730491468120135 34.0 31.0 34.0 31.0 34.0 4 36.289750432564006 37.0 35.0 37.0 35.0 37.0 5 36.2206357119815 37.0 35.0 37.0 35.0 37.0 6 36.25883456992874 37.0 37.0 37.0 35.0 37.0 7 36.21345075054568 37.0 36.0 37.0 35.0 37.0 8 36.19339570075369 37.0 36.0 37.0 35.0 37.0 9 37.92068808050509 39.0 38.0 39.0 35.0 39.0 10-11 37.85308534393552 39.0 38.0 39.0 35.0 39.0 12-13 37.768447755066134 39.0 37.5 39.0 35.0 39.0 14-15 39.065683953865424 40.0 38.0 41.0 36.0 41.0 16-17 39.02963136748878 40.0 38.0 41.0 36.0 41.0 18-19 38.99395290184463 40.0 38.0 41.0 36.0 41.0 20-21 38.88354539094151 40.0 38.0 41.0 35.0 41.0 22-23 38.730452505938594 40.0 38.0 41.0 35.0 41.0 24-25 38.81417092248202 40.0 38.0 41.0 35.0 41.0 26-27 38.70614932151341 40.0 38.0 41.0 35.0 41.0 28-29 38.569533249822996 40.0 38.0 41.0 34.0 41.0 30-31 38.64273103945236 40.0 38.0 41.0 35.0 41.0 32-33 38.599069516072944 40.0 38.0 41.0 35.0 41.0 34-35 38.488663680962574 40.0 38.0 41.0 35.0 41.0 36-37 38.34975093530183 40.0 38.0 41.0 34.0 41.0 38-39 38.222880017428245 40.0 38.0 41.0 34.0 41.0 40-41 38.06424402894094 40.0 38.0 41.0 34.0 41.0 42-43 37.86804263216768 40.0 38.0 41.0 33.0 41.0 44-45 37.58498782955512 40.0 37.0 41.0 33.0 41.0 46-47 37.272134499126494 40.0 37.0 41.0 32.5 41.0 48-49 36.984305362955766 40.0 37.0 41.0 31.5 41.0 50-51 36.743151244485595 40.0 36.5 41.0 31.0 41.0 52-53 36.52329687087598 39.0 36.0 41.0 31.0 41.0 54-55 36.30384636332025 39.0 36.0 40.5 30.0 41.0 56-57 36.05299317533401 39.0 35.0 40.0 30.0 41.0 58-59 35.79189628518641 39.0 35.0 40.0 29.0 41.0 60-61 35.43820304742913 38.0 35.0 40.0 28.0 41.0 62-63 35.094272978260776 38.0 34.0 40.0 28.0 41.0 64-65 34.71721583791732 37.5 34.0 40.0 27.5 41.0 66-67 34.29421264972161 37.0 34.0 39.5 26.0 41.0 68-69 33.84773956504799 36.0 33.0 39.0 26.0 40.0 70-71 33.41881077367162 36.0 33.0 39.0 26.0 40.0 72-73 32.91756356491393 35.0 32.5 38.0 25.0 40.0 74-75 32.41269329222055 35.0 32.0 37.0 23.5 39.0 76-77 31.18574738262119 34.0 30.5 36.0 22.5 38.5 78-79 31.776404000117303 34.5 31.5 36.0 24.0 39.0 80-81 31.559250166531903 35.0 31.5 36.0 23.0 37.5 82-83 31.19775653244963 34.0 31.0 35.5 21.5 37.0 84-85 30.799595714997928 34.0 31.0 35.0 20.0 37.0 86-87 30.37217346130804 34.0 31.0 35.0 18.0 36.0 88-89 30.009304420322337 34.0 30.0 35.0 16.0 36.0 90-91 29.48245947723645 34.0 29.5 35.0 7.5 35.5 92-93 29.00922775280381 34.0 29.0 35.0 2.0 35.0 94-95 28.535235218460535 34.0 29.0 35.0 2.0 35.0 96-97 27.991244401804828 33.5 29.0 35.0 2.0 35.0 98-99 27.116494828084612 33.0 26.5 35.0 2.0 35.0 100-101 24.42735103249781 31.0 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.20146388558377026 1101 2 0.16986543956675604 1101 3 0.11683067509817135 1101 4 0.09935151580707924 1101 5 0.09731818405614234 1101 6 0.08468672804018951 1101 7 0.13675427130583984 1101 8 0.11307882372275202 1101 9 0.1403768148955109 1101 10-11 0.11800051282529722 1101 12-13 0.22592457933465937 1101 14-15 0.2267659291018802 1101 16-17 0.20448423281150951 1101 18-19 0.22569551313647196 1101 20-21 0.1616408217946983 1101 22-23 0.11147160419925939 1101 24-25 0.19640270781781766 1101 26-27 0.22942775972516216 1101 28-29 0.13957854149440152 1101 30-31 0.11914689896768493 1101 32-33 0.18190356618092096 1101 34-35 0.2487323637476564 1101 36-37 0.22456177759465135 1101 38-39 0.16285959260925864 1101 40-41 0.1044675257730674 1101 42-43 0.1899654359392713 1101 44-45 0.26881108332840853 1101 46-47 0.23449500231903642 1101 48-49 0.27484986282278356 1101 50-51 0.2844862133109558 1101 52-53 0.2579830339012119 1101 54-55 0.24960285815384253 1101 56-57 0.3769428895818763 1101 58-59 0.28748006972191575 1101 60-61 0.25294805790347397 1101 62-63 0.36059272736442693 1101 64-65 0.2602264156729319 1101 66-67 0.24378293905395054 1101 68-69 0.17332553878534895 1101 70-71 0.21000734598125348 1101 72-73 0.059941056665927306 1101 74-75 0.27756077968309967 1101 76-77 0.18152895354713294 1101 78-79 0.179794148244401 1101 80-81 0.19557022724537276 1101 82-83 0.220367880614873 1101 84-85 0.2133586809858663 1101 86-87 0.26512354879479716 1101 88-89 0.19616669146769894 1101 90-91 0.13461868159030033 1101 92-93 0.19410841547446722 1101 94-95 0.26772601859471834 1101 96-97 0.6757345562757742 1101 98-99 0.6968418267106493 1101 100-101 0.8071638389656499 1102 1 0.13664672851388104 1102 2 0.18304001512169776 1102 3 0.14784722300844777 1102 4 0.0931144290113366 1102 5 0.06448483147028128 1102 6 0.06074804172522619 1102 7 0.0977481694741229 1102 8 0.0331935200275808 1102 9 0.1139136108562866 1102 10-11 0.1399567983845813 1102 12-13 0.07719115173706825 1102 14-15 0.15635030852965315 1102 16-17 0.1520838573141745 1102 18-19 0.08282155478334374 1102 20-21 0.1570626202339227 1102 22-23 0.20535507719868917 1102 24-25 0.08897508390589337 1102 26-27 0.1188289526018309 1102 28-29 0.10604003473969215 1102 30-31 0.032870610133031164 1102 32-33 -0.016418027986588868 1102 34-35 -0.02488024816678802 1102 36-37 0.07030665288947091 1102 38-39 0.14181755825593 1102 40-41 0.2527231739519493 1102 42-43 0.2998598200022755 1102 44-45 0.14645532007305206 1102 46-47 0.006569727458789032 1102 48-49 -0.032779766373600694 1102 50-51 -0.16278477791135515 1102 52-53 -0.2058077199155406 1102 54-55 -0.2026184688666106 1102 56-57 -0.2267038066118232 1102 58-59 -0.16892721913759345 1102 60-61 -0.3654019617633466 1102 62-63 -0.4202717499734021 1102 64-65 -0.27387796907731854 1102 66-67 -0.03207015492420595 1102 68-69 -0.06677123073853863 1102 70-71 -0.22409309078297923 1102 72-73 -0.027607360995894226 1102 74-75 -0.08686135945269768 1102 76-77 -0.2714271375372945 1102 78-79 -0.07623164818866357 1102 80-81 -0.0970088019302402 1102 82-83 -0.10756170778564922 1102 84-85 -0.07968898875194341 1102 86-87 0.030701278059460435 1102 88-89 -0.03166463594756053 1102 90-91 0.12535224663629307 1102 92-93 0.11104571414545816 1102 94-95 -0.013483176410932884 1102 96-97 0.026052515371716822 1102 98-99 -0.20015814258279363 1102 100-101 0.4146843136597944 1103 1 0.16919062445252564 1103 2 0.15709307757730073 1103 3 0.13528704276026104 1103 4 0.0889386903250653 1103 5 0.06181061160903312 1103 6 0.07538991053105093 1103 7 0.04425800351591391 1103 8 0.03289519411394082 1103 9 0.014049423316414789 1103 10-11 0.0382705152782421 1103 12-13 0.029955528656202546 1103 14-15 0.11650937722537691 1103 16-17 0.06776269710656635 1103 18-19 0.07953461305248766 1103 20-21 0.04511870550568631 1103 22-23 0.15712599586120035 1103 24-25 0.1547225716226066 1103 26-27 -0.03205761927081596 1103 28-29 -0.0013552339102176347 1103 30-31 0.03910784681096402 1103 32-33 -0.00999660390064605 1103 34-35 0.055308850905440465 1103 36-37 -0.07578610174653022 1103 38-39 -0.2754464469919711 1103 40-41 -0.35499555580597786 1103 42-43 -0.252187338567019 1103 44-45 -0.2349308735355251 1103 46-47 -0.21328410932562036 1103 48-49 -0.43776955571595266 1103 50-51 -0.4246580668639055 1103 52-53 -0.4894332533173298 1103 54-55 -0.5043133226621919 1103 56-57 -0.2785972696482517 1103 58-59 -0.24389973524472452 1103 60-61 -0.4535296617364821 1103 62-63 -0.2486692453542929 1103 64-65 -0.1569445122381552 1103 66-67 -0.24332651907300828 1103 68-69 -0.35489143135859536 1103 70-71 -0.25083693760497994 1103 72-73 -0.1651335469238191 1103 74-75 -0.13182286552057576 1103 76-77 -0.23744188241919062 1103 78-79 0.008387425880219013 1103 80-81 -0.08904795020151468 1103 82-83 0.016247666900159885 1103 84-85 -0.2111069103927683 1103 86-87 -0.18027612663694725 1103 88-89 -0.38335009494917216 1103 90-91 -0.14601143243095294 1103 92-93 -0.219996333326268 1103 94-95 -0.3092968067735882 1103 96-97 -0.3094438181863204 1103 98-99 -0.1516105447656848 1103 100-101 0.19730649954815505 1104 1 0.26741026413519364 1104 2 0.20850489534906558 1104 3 0.1288995026291886 1104 4 0.10224867467062637 1104 5 0.07023535092288569 1104 6 0.06062910499097285 1104 7 0.03208840495930332 1104 8 0.04660423388681778 1104 9 0.21795984810879787 1104 10-11 0.13440307384970396 1104 12-13 0.20299540766599478 1104 14-15 0.12461306795741933 1104 16-17 0.24733737406801737 1104 18-19 0.12428294811046214 1104 20-21 0.1842778766166262 1104 22-23 0.3140126206058227 1104 24-25 0.2725171535961337 1104 26-27 0.1511804608055911 1104 28-29 0.05422039823709213 1104 30-31 0.2619320130133076 1104 32-33 0.31594518282757633 1104 34-35 0.28942464065841733 1104 36-37 0.33014401048780684 1104 38-39 0.4527795605964471 1104 40-41 0.46248673345613867 1104 42-43 0.4309658360993325 1104 44-45 0.26542572918675233 1104 46-47 0.2675002043998731 1104 48-49 0.1318926799496296 1104 50-51 0.37968829414329264 1104 52-53 0.3016965192953691 1104 54-55 0.21515707984251264 1104 56-57 0.3333494522069316 1104 58-59 0.4414336511544903 1104 60-61 0.17462072697109932 1104 62-63 0.22988444405110187 1104 64-65 0.28279944488461695 1104 66-67 0.3155556481375825 1104 68-69 0.12220108900542925 1104 70-71 0.12280046322815252 1104 72-73 0.15976098463711708 1104 74-75 0.043687230263330434 1104 76-77 0.1349023028868679 1104 78-79 0.22656885812835625 1104 80-81 0.3350580223634232 1104 82-83 0.46763878897822053 1104 84-85 0.11873283064572959 1104 86-87 0.18630802258431345 1104 88-89 0.3320090284024708 1104 90-91 0.16129335145824797 1104 92-93 0.10502078042044616 1104 94-95 0.0857492278784342 1104 96-97 0.3640298744030268 1104 98-99 0.29815835332129126 1104 100-101 0.7396968521709368 1105 1 0.21489677551826958 1105 2 0.18433263584123694 1105 3 0.1955648533332237 1105 4 0.057392114596936494 1105 5 0.080523546738128 1105 6 0.08630616776238753 1105 7 0.07639489265505262 1105 8 0.10876636048242005 1105 9 0.27360795970242435 1105 10-11 0.09443202502749415 1105 12-13 0.19511443648556792 1105 14-15 0.1874048826698882 1105 16-17 0.19865958751714885 1105 18-19 0.25876511681687475 1105 20-21 0.3982441879733827 1105 22-23 0.2693735233561867 1105 24-25 0.2208791091165878 1105 26-27 0.11613999495482119 1105 28-29 0.11901477907667868 1105 30-31 0.13527784799158837 1105 32-33 0.21936569868492484 1105 34-35 0.19462565394963605 1105 36-37 -0.04046889910556217 1105 38-39 -0.07550672707298389 1105 40-41 0.04310556488132278 1105 42-43 0.02077074463993256 1105 44-45 -0.07687786339121772 1105 46-47 -0.1700794113390387 1105 48-49 0.0842877319301536 1105 50-51 0.10241858760296196 1105 52-53 -0.05714049873331817 1105 54-55 -0.13698399135105888 1105 56-57 -0.09733188744302623 1105 58-59 -0.07991521053783401 1105 60-61 -0.004943689399475204 1105 62-63 -0.09152100403417762 1105 64-65 0.09045867636244509 1105 66-67 0.06443905384885795 1105 68-69 -0.06741958476742838 1105 70-71 0.006748022117477603 1105 72-73 -0.032782442129565936 1105 74-75 -0.11078046285417997 1105 76-77 0.16400415839416738 1105 78-79 0.2662760564439246 1105 80-81 0.03108480220287646 1105 82-83 0.1969051398138575 1105 84-85 0.20789357548057907 1105 86-87 0.25961010180526145 1105 88-89 0.24895106024876412 1105 90-91 0.2653813893579695 1105 92-93 0.4447947638393188 1105 94-95 0.28872132947075 1105 96-97 0.5273589308578224 1105 98-99 0.514995766138771 1105 100-101 0.4922185655542748 1106 1 0.060106483023893986 1106 2 0.1581897091026505 1106 3 0.19248959196004733 1106 4 0.09734727571562729 1106 5 0.08089212939358958 1106 6 0.05069146804507341 1106 7 0.05184007640151833 1106 8 0.10363665143836442 1106 9 0.006774950080817632 1106 10-11 0.012135530729302957 1106 12-13 -0.0664129860639946 1106 14-15 0.10746491356672294 1106 16-17 0.31622663750977864 1106 18-19 0.09437564994765069 1106 20-21 0.04267891038256266 1106 22-23 0.14965507242896336 1106 24-25 0.2554116982334733 1106 26-27 0.2568908939759993 1106 28-29 0.2993218084286795 1106 30-31 0.28961221976649654 1106 32-33 0.2860435029579236 1106 34-35 0.187652630994954 1106 36-37 0.2373522806153403 1106 38-39 0.226769110571162 1106 40-41 0.3997056783406876 1106 42-43 0.32656334157717026 1106 44-45 0.3655695574917175 1106 46-47 0.5154646967870846 1106 48-49 0.6647877864162837 1106 50-51 0.7657684110037835 1106 52-53 0.6455787598706806 1106 54-55 0.7112849521948945 1106 56-57 0.5965069329642745 1106 58-59 0.6256305161469058 1106 60-61 0.2823087993347144 1106 62-63 0.5247684262972996 1106 64-65 0.6007577571493528 1106 66-67 0.4366939143323165 1106 68-69 0.5291486703302297 1106 70-71 0.7692513874373788 1106 72-73 0.7785204655068227 1106 74-75 0.7092133834180032 1106 76-77 0.4493312033868939 1106 78-79 0.6923455871580764 1106 80-81 0.8174963615959676 1106 82-83 0.6221317380203004 1106 84-85 0.694114593666125 1106 86-87 0.6462192535523421 1106 88-89 0.5142260068844067 1106 90-91 0.7274678556438765 1106 92-93 0.6427408770550862 1106 94-95 0.9863481379295571 1106 96-97 0.8067626121972857 1106 98-99 0.611718665698394 1106 100-101 0.8254938070560414 1107 1 0.19025458258546735 1107 2 0.1966173451482618 1107 3 0.11365735757839701 1107 4 0.1115139711506643 1107 5 0.12248198451608516 1107 6 0.081025986635197 1107 7 0.09457836479865733 1107 8 0.06952887362223237 1107 9 0.12238696835448337 1107 10-11 0.14639693163378098 1107 12-13 0.24087634317272233 1107 14-15 0.10777158437603163 1107 16-17 0.16750510381051242 1107 18-19 0.1555751758449304 1107 20-21 0.122255670143673 1107 22-23 0.2906367642391743 1107 24-25 0.19062015383236997 1107 26-27 0.20862907880701442 1107 28-29 0.19355892883005765 1107 30-31 0.24050749139742322 1107 32-33 0.14513488280395848 1107 34-35 0.28280285941580274 1107 36-37 -0.008764564990570989 1107 38-39 0.14937299315018748 1107 40-41 0.38721387732573476 1107 42-43 0.41894835440786693 1107 44-45 0.2783881671739934 1107 46-47 0.24394480633703353 1107 48-49 -0.008527825601987615 1107 50-51 -0.10217326975938334 1107 52-53 -0.14187305311190812 1107 54-55 -0.1245759700039315 1107 56-57 0.11674893248096652 1107 58-59 0.08899802630214282 1107 60-61 0.07210005605212899 1107 62-63 -0.01932887983267051 1107 64-65 0.22280180727535281 1107 66-67 0.17857950828405222 1107 68-69 0.15566907861091295 1107 70-71 0.25092708737178526 1107 72-73 0.3267701979609967 1107 74-75 0.010259401300423576 1107 76-77 0.00551179969763993 1107 78-79 0.17258114256020107 1107 80-81 0.11922764201851521 1107 82-83 -0.1484514543480273 1107 84-85 -0.021985336139056955 1107 86-87 0.2594878005195831 1107 88-89 0.3886969565857932 1107 90-91 0.3712579155971554 1107 92-93 0.48260405469401135 1107 94-95 0.6280053110345882 1107 96-97 0.49850896724498384 1107 98-99 0.7665897258703147 1107 100-101 1.2932404382800797 1108 1 0.18790937286959775 1108 2 0.28436942297830115 1108 3 0.17303497499995046 1108 4 0.09590286361893163 1108 5 0.10895192846299295 1108 6 0.18340985365407647 1108 7 0.129485909415628 1108 8 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2316 98-99 -0.007663349700866462 2316 100-101 0.025151209660855045 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 6.0 5 24.0 6 32.0 7 74.0 8 198.0 9 371.0 10 684.0 11 1090.0 12 1795.0 13 2670.0 14 3566.0 15 4980.0 16 7161.0 17 10518.0 18 9799.0 19 5786.0 20 4786.0 21 4794.0 22 5086.0 23 5672.0 24 6446.0 25 7513.0 26 9242.0 27 11554.0 28 14179.0 29 18168.0 30 23121.0 31 29987.0 32 39335.0 33 53570.0 34 76052.0 35 113454.0 36 181905.0 37 284823.0 38 233130.0 39 21891.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.85635267437358 19.71850816366553 14.999404968429802 22.425734193531085 2 15.369449460185258 20.384426857930478 40.65481601890294 23.591307662981322 3 19.67363936102022 24.051228984511486 30.30956081661381 25.965570837854486 4 13.023456130149096 17.030956997374027 37.23470039263923 32.71088647983765 5 13.660156682120336 37.48621405177818 34.561162679217325 14.292466586884158 6 32.29168848688483 36.552223986459595 17.071887319695172 14.084200206960404 7 28.67516014294512 32.91315622996904 20.508519311416755 17.90316431566908 8 25.88873574005103 36.217903331894945 19.318203717746226 18.5751572103078 9 25.954845764224338 17.370346009309028 20.5706912226165 36.104117003850135 10-11 24.965876670032216 27.117720251536493 27.189486076256948 20.726917002174343 12-13 25.71987582063539 24.15929648451738 28.171035707851704 21.94979198699552 14-15 22.360479779465674 25.831758786390886 26.462820442995817 25.344940991147624 16-17 21.79783236207178 29.232361233886206 27.16263149736272 21.80717490667929 18-19 21.696446900411825 27.776013540405458 29.55591492000184 20.97162463918087 20-21 22.26521096136041 26.826006627760346 29.794170754902744 21.114611655976507 22-23 22.05594446024828 26.687510971329793 29.721693759654244 21.53485080876768 24-25 22.051296016221674 26.861072591152652 29.88122818850993 21.206403204115748 26-27 21.950537952450038 27.02477375665135 29.754357182328196 21.270331108570414 28-29 21.527624161253776 27.261891594774134 29.94089394762068 21.269590296351414 30-31 21.876008094079005 26.902464672193986 29.82454449858186 21.396982735145144 32-33 21.76021081472854 27.005358347333186 29.709710800065352 21.524720037872918 34-35 22.137347974176034 27.03594156510664 29.57862191182816 21.24808854888916 36-37 22.12415110623269 26.748585002492742 29.66869577239383 21.458568118880738 38-39 21.855111735623474 27.15392875112749 29.714750627270437 21.276208885978594 40-41 21.959413601194516 27.07691289316555 29.63574632956109 21.32792717607884 42-43 21.94501907994722 27.080828832739655 29.362377369385012 21.61177471792811 44-45 22.03797346382173 27.085545030646056 29.233366709539034 21.643114795993178 46-47 22.240283544134094 27.525398733938573 28.779729610839027 21.454588111088302 48-49 22.313473792696055 27.67240765334551 28.483407556987427 21.530710996971006 50-51 22.061937300214083 27.6913860062926 28.593590930609615 21.653085762883702 52-53 22.31619695592246 28.006141780446015 28.069277272479713 21.608383991151815 54-55 22.09017440813095 27.861018127888126 28.21314408047157 21.835663383509363 56-57 22.221891718651154 27.97953857046499 28.302589518754218 21.49598019212964 58-59 22.46132060848035 27.78347081816392 28.153988596230306 21.60121997712543 60-61 22.301575664137616 27.742916633499938 28.597738517677517 21.35776918468493 62-63 22.17928468786265 27.70148265763973 28.640052284733947 21.47918036976367 64-65 22.057203185682024 27.920215506948253 28.417255637995247 21.605325669374466 66-67 22.051296016221674 27.90169799700871 28.244020561977102 21.802985424792514 68-69 21.9479087982927 27.867607289533957 28.380274402799582 21.80420950937376 70-71 22.205175685922924 27.83726376559011 28.191652038392412 21.765908510094555 72-73 22.226500148726608 27.745346532994265 28.286376223852393 21.74177709442673 74-75 22.05175685922922 28.185158341467908 27.918791083106754 21.844293716196116 76-77 22.089881144398873 27.916989605895438 28.127930018894563 21.865199230811125 78-79 22.176058786809836 27.9206763499558 27.937266698227432 21.965998165006933 80-81 22.16437013234573 28.132370869694544 27.91598413024261 21.78727486771711 82-83 22.14533886748249 28.138819051002628 27.921673383253516 21.79416869826137 84-85 22.0668895480368 28.08707455633201 27.852881537713873 21.993154357917316 86-87 22.034998931682118 28.07602233831742 27.92943236709916 21.9595463629013 88-89 22.086320084795112 28.061317256894842 27.713380786198172 22.138981872111877 90-91 22.030645722210814 27.973041485274212 27.85081893716244 22.145493855352527 92-93 22.340335500737364 27.72661382222818 27.832274768735754 22.1007759082987 94-95 22.05577876184052 27.763068041375323 27.84551704490706 22.335636151877097 96-97 21.93273367882594 27.826035954133555 28.006141780446015 22.235088586594497 98-99 22.054197133579038 28.05621902073796 27.556277465259026 22.333306380423977 100-101 21.68812072659556 28.38535763028842 27.56224079865338 22.364280844462638 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 151.0 1 112.0 2 107.0 3 149.5 4 131.0 5 125.0 6 236.5 7 517.0 8 870.0 9 1116.0 10 1167.5 11 1174.0 12 1274.5 13 1432.0 14 1545.0 15 1648.0 16 1801.0 17 1910.0 18 2007.0 19 2111.5 20 2354.5 21 2700.0 22 3154.0 23 3800.5 24 4575.5 25 5696.0 26 7176.0 27 8941.0 28 10470.0 29 12182.5 30 14351.5 31 16548.0 32 18761.5 33 21448.5 34 24583.5 35 27057.5 36 29920.5 37 32677.0 38 34985.5 39 36893.5 40 38013.0 41 40324.5 42 42795.5 43 44454.5 44 46461.0 45 47784.5 46 47680.0 47 48839.0 48 51842.0 49 50746.5 50 46724.0 51 43357.5 52 40347.0 53 37895.5 54 35011.0 55 32084.0 56 28761.0 57 25539.5 58 22999.5 59 19767.5 60 15938.0 61 12417.5 62 9469.5 63 7257.0 64 5451.0 65 4046.0 66 2890.0 67 2175.5 68 1536.5 69 921.5 70 642.5 71 434.0 72 320.5 73 236.5 74 157.0 75 122.0 76 85.5 77 52.0 78 35.0 79 20.5 80 12.5 81 7.5 82 6.5 83 5.0 84 5.0 85 4.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021031199071610815 2 0.0 3 0.0 4 2.5136891320650377E-4 5 0.018517509939545776 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0025136891320650377 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001131160109429267 24-25 0.0 26-27 3.7705336980975565E-4 28-29 0.0014244238415035213 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 4.189481886775063E-5 40-41 9.216860150905137E-4 42-43 0.016464663815025996 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.189481886775063E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.002220425399990783 84-85 4.189481886775062E-4 86-87 0.0 88-89 0.0 90-91 0.012568445660325188 92-93 0.0020947409433875316 94-95 0.0 96-97 0.0 98-99 0.002136635762255282 100-101 0.02149204207915607 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1193465.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.333086984158236 #Duplication Level Percentage of deduplicated Percentage of total 1 79.64799331927514 49.64705295684032 2 12.117018173613534 15.105822956089574 3 3.62609838277401 6.780777177197036 4 1.5793967731088518 3.937947057627716 5 0.8373364411979981 2.609688261210016 6 0.4848690208651216 1.8134029712105557 7 0.33017187876424436 1.4406442707114215 8 0.2402857285647451 1.1982200975742474 9 0.16143481425066022 0.9056457287062049 >10 0.8944238733983213 10.07445752685209 >50 0.05545183081716754 2.3335456692449785 >100 0.02416951608959313 2.6402646527288494 >500 6.751236402597354E-4 0.2708432312589399 >1k 6.751236402597354E-4 1.2416874427480238 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4665 0.3908786600361133 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4170 0.34940278935704017 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2688 0.22522654623302737 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2148 0.1799801418558567 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 0.0 0.0 24-25 0.0 0.0 0.0 0.0 0.0 26-27 0.0 0.0 0.0 2.5136891320650377E-4 0.0 28-29 0.0 0.0 0.0 0.001005475652826015 0.0 30-31 0.0 0.0 0.0 0.00402190261130406 0.0 32-33 0.0 0.0 0.0 0.0072059088452531075 0.0 34-35 0.0 0.0 0.0 0.009719597977318145 0.0 36-37 0.0 0.0 0.0 0.013992869501828709 0.0 38-39 0.0 0.0 0.0 0.02153393689802382 0.0 40-41 0.0 0.0 0.0 0.0302061644036482 0.0 42-43 0.0 0.0 0.0 0.038962181547008076 0.0 44-45 0.0 0.0 0.0 0.04298408415831215 0.0 46-47 0.0 0.0 0.0 0.04897504325640048 0.0 48-49 0.0 0.0 0.0 0.05835948268277662 0.0 50-51 0.0 0.0 0.0 0.06795339620349151 0.0 52-53 0.0 0.0 0.0 0.08399911182984 0.0 54-55 0.0 0.0 0.0 0.09774061241846221 0.0 56-57 0.0 0.0 0.0 0.13532026494283453 0.0 58-59 0.0 0.0 0.0 0.19204584968976887 0.0 60-61 0.0 0.0 0.0 0.2451265851952089 0.0 62-63 0.0 0.0 0.0 0.27717612162903815 0.0 64-65 0.0 0.0 0.0 0.3321840188023947 0.0 66-67 0.0 0.0 0.0 0.42292819646994256 0.0 68-69 0.0 0.0 0.0 0.5514615007562014 0.0 70-71 0.0 0.0 0.0 0.6859857641405488 0.0 72-73 0.0 0.0 0.0 0.837980166992748 0.0 74-75 0.0 0.0 0.0 1.0093299761618482 0.0 76-77 0.0 0.0 0.0 1.1444407670103438 0.0 78-79 0.0 0.0 0.0 1.2933349532663296 0.0 80-81 0.0 0.0 0.0 1.4427318773487283 0.0 82-83 0.0 0.0 0.0 1.6055351434688072 0.0 84-85 0.0 0.0 0.0 1.7924279304378428 0.0 86-87 0.0 0.0 0.0 2.070023000255558 0.0 88-89 0.0 0.0 0.0 2.453025434344535 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCGAA 30 0.0039529228 31.664825 48-49 ATACCGT 95 3.4923418E-5 29.998255 6 ACCGCGC 70 0.00839065 27.143553 8 GTACATG 3510 0.0 23.825262 1 GGTATCA 5565 0.0 23.650848 1 GTATCAA 7155 0.0 23.442135 1 ACCGCTC 165 2.4294714E-6 23.030891 8 AGTACTT 4810 0.0 21.973015 12-13 TACATGG 3790 0.0 21.934198 2 GAGTACT 4540 0.0 21.658043 12-13 TAGTACC 265 7.930794E-10 21.510885 4 ACATGGG 3690 0.0 21.49874 3 GACGTCG 70 4.8557497E-4 20.355957 46-47 GTACTTT 4820 0.0 20.34892 14-15 CATGGGG 1855 0.0 19.974394 4 AGAGTAC 6925 0.0 19.892204 10-11 ACCGTCC 120 0.004762898 19.792173 8 TCAACGC 8410 0.0 19.712982 4 ATCAACG 8490 0.0 19.52723 3 TACACCG 195 1.0320995E-5 19.488495 5 >>END_MODULE