FastQCFastQC Report
Thu 26 May 2016
SRR937677_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937677_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079585
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32900.3047467313828925No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27070.250744499043614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17080.15820894139877822No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14220.13171728025120766No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14100.13060574202123965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31800.028.4118981
GTACATG32950.026.2657471
GTATCAA43100.025.3760681
TACATGG34050.024.150222
CGACTTA500.001654202623.74977914-15
ACATGGG34200.023.2039263
GGACCGC1100.002875854721.5927146
TCAACGC51800.020.7343354
ATCAACG52400.020.5857013
CAACGCA52550.020.4384125
GGACCGA1404.8487308E-420.3588456
TATAGGG2351.123517E-720.2266642
TATCAAC53950.020.0879462
AACGCAG53800.019.95526
GAGTACT31700.019.10471212-13
AGAGTAC49600.018.91407410-11
AGTACTT33200.018.67076112-13
CTTTGCG1559.5796556E-418.399742
CATGGGG18600.018.396324
TAGACTG3951.8189894E-1118.0470225
Produced by FastQC (version 0.11.5)