##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937677_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1079585 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.781031600105596 33.0 31.0 34.0 30.0 34.0 2 31.933792151613815 33.0 31.0 34.0 30.0 34.0 3 31.95810427154879 34.0 31.0 34.0 30.0 34.0 4 35.53770569246516 37.0 35.0 37.0 33.0 37.0 5 35.51799626708411 37.0 35.0 37.0 33.0 37.0 6 35.55879898294252 37.0 35.0 37.0 33.0 37.0 7 35.50540439150229 37.0 35.0 37.0 33.0 37.0 8 35.48649897877425 37.0 35.0 37.0 33.0 37.0 9 37.13017872608456 39.0 37.0 39.0 34.0 39.0 10-11 37.01095281983355 39.0 37.0 39.0 33.0 39.0 12-13 36.88254236581649 39.0 37.0 39.0 33.0 39.0 14-15 38.06632270733662 40.0 38.0 41.0 33.0 41.0 16-17 37.99192467475928 40.0 38.0 41.0 33.0 41.0 18-19 37.90497228101539 40.0 38.0 41.0 33.0 41.0 20-21 37.81170310813877 40.0 38.0 41.0 33.0 41.0 22-23 37.605031563054325 40.0 37.5 41.0 32.0 41.0 24-25 37.43364765164392 40.0 37.0 41.0 32.0 41.0 26-27 37.378035078293976 39.5 37.0 41.0 32.0 41.0 28-29 37.43860742785422 40.0 37.0 41.0 32.0 41.0 30-31 37.28869982446959 40.0 37.0 41.0 32.0 41.0 32-33 37.0717761917774 39.0 36.5 41.0 31.0 41.0 34-35 37.12186303070161 40.0 37.0 41.0 31.0 41.0 36-37 37.051265995729835 40.0 37.0 41.0 31.0 41.0 38-39 36.93233649967348 40.0 36.0 41.0 31.0 41.0 40-41 36.71831398176151 39.5 36.0 41.0 30.0 41.0 42-43 36.492690709856106 39.0 36.0 41.0 30.0 41.0 44-45 36.2969307650625 39.0 36.0 41.0 30.0 41.0 46-47 35.99695577467267 39.0 35.0 41.0 29.0 41.0 48-49 35.68167907112455 39.0 35.0 40.0 27.5 41.0 50-51 34.78198335471501 38.0 34.0 39.5 26.0 40.5 52-53 34.90250976069508 38.0 34.5 39.5 26.5 40.5 54-55 35.25599095948906 39.0 35.0 40.0 27.0 41.0 56-57 35.04719313439887 38.5 34.5 40.0 26.0 41.0 58-59 34.79610081651746 38.0 34.0 40.0 26.0 41.0 60-61 34.464597044234594 38.0 34.0 40.0 25.0 41.0 62-63 34.10183728006595 38.0 34.0 40.0 23.5 41.0 64-65 33.73030053214893 37.0 33.0 40.0 22.5 41.0 66-67 33.31371869746245 36.5 33.0 39.0 22.0 41.0 68-69 32.8135686398014 36.0 32.0 39.0 20.5 40.5 70-71 32.35843124904477 35.5 31.5 39.0 18.5 40.0 72-73 31.897922812932748 35.0 31.0 38.0 17.5 40.0 74-75 31.412956830633995 35.0 31.0 37.0 13.0 39.5 76-77 30.90005465062964 35.0 30.5 37.0 8.0 39.0 78-79 30.337270339991754 34.0 30.0 36.0 7.0 39.0 80-81 29.778861784852513 34.0 29.0 36.0 2.0 37.5 82-83 29.229388144518495 34.0 29.0 35.0 2.0 37.0 84-85 28.734209904731912 34.0 29.0 35.0 2.0 36.5 86-87 28.23701653876258 34.0 27.5 35.0 2.0 36.0 88-89 27.725572326403203 33.0 26.5 35.0 2.0 36.0 90-91 27.19487256677334 33.0 26.0 35.0 2.0 35.0 92-93 26.603311457643446 32.5 25.0 35.0 2.0 35.0 94-95 25.920163766632548 32.0 23.5 34.5 2.0 35.0 96-97 25.15276147779008 32.0 19.5 34.0 2.0 35.0 98-99 24.150058124186607 31.5 7.0 34.0 2.0 35.0 100-101 21.69630043025792 28.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11253036775819325 1101 2 0.06825404412431268 1101 3 0.02484422212403814 1101 4 0.10663156540783092 1101 5 -0.12630274641592365 1101 6 0.02483729361021858 1101 7 0.06971342096790778 1101 8 -0.09984410757979845 1101 9 0.0655177912245648 1101 10-11 -0.10828384519871292 1101 12-13 0.12962787295264633 1101 14-15 0.19436931969183036 1101 16-17 0.1634182740315282 1101 18-19 0.11976714714856485 1101 20-21 0.24122241563900815 1101 22-23 0.2265579206642201 1101 24-25 0.024987379411399502 1101 26-27 0.1703260217874174 1101 28-29 -0.032803856146337296 1101 30-31 0.00958290331575995 1101 32-33 -0.07111344331919867 1101 34-35 0.1853037627982772 1101 36-37 0.1325817266085565 1101 38-39 0.0800901313183715 1101 40-41 0.19255718556402002 1101 42-43 0.04283706030670942 1101 44-45 0.09189395902775033 1101 46-47 0.3255742539959954 1101 48-49 0.1454623374664763 1101 50-51 0.002113149947014392 1101 52-53 0.05153341257499022 1101 54-55 0.11491909306096915 1101 56-57 0.03869279795770808 1101 58-59 0.1846297714808074 1101 60-61 0.2404840289577308 1101 62-63 0.14159741676122195 1101 64-65 0.24707129925543825 1101 66-67 0.25691080757570006 1101 68-69 0.2331088106857493 1101 70-71 0.24676611371297952 1101 72-73 0.20288874928763434 1101 74-75 0.4755046390012865 1101 76-77 0.699853139416188 1101 78-79 0.6387901384719648 1101 80-81 0.43175423335314633 1101 82-83 0.4290347548198703 1101 84-85 0.831305971274638 1101 86-87 1.0171249311316508 1101 88-89 0.8417060042156059 1101 90-91 0.8358312302724045 1101 92-93 0.7780747890648705 1101 94-95 0.7795838444229375 1101 96-97 1.2394422599703354 1101 98-99 1.2412002156001805 1101 100-101 1.8315791661698668 1102 1 -0.023788620516100423 1102 2 -0.16933343822573832 1102 3 -0.071808426902912 1102 4 -0.12802832777161655 1102 5 -0.01643565819687609 1102 6 -0.02751398639469471 1102 7 -0.2187864869454117 1102 8 -0.05370100487245111 1102 9 0.12152567998384711 1102 10-11 0.1582361222639861 1102 12-13 -0.01201295828315807 1102 14-15 0.14081899431887734 1102 16-17 0.10728376608138035 1102 18-19 0.02310874270384744 1102 20-21 0.15060911832179613 1102 22-23 0.17252989561663412 1102 24-25 0.1361398442649815 1102 26-27 0.13975132413869318 1102 28-29 0.054998878828193654 1102 30-31 0.2440490307637333 1102 32-33 0.13408638478565393 1102 34-35 0.16946293463202977 1102 36-37 0.15916216432726316 1102 38-39 0.3766399502145603 1102 40-41 0.14689370100388288 1102 42-43 0.19546882100411267 1102 44-45 0.2997604640311309 1102 46-47 0.28798753903497953 1102 48-49 0.26877600006086055 1102 50-51 0.16505861928214927 1102 52-53 -0.05749338465012954 1102 54-55 0.15079453047272295 1102 56-57 0.03602995800436304 1102 58-59 0.1868014824514006 1102 60-61 0.18457781924565353 1102 62-63 0.2290893591763563 1102 64-65 0.30092858024261204 1102 66-67 0.34254375005221505 1102 68-69 0.2756010600564949 1102 70-71 0.3972952284529896 1102 72-73 0.4843440258546501 1102 74-75 0.5204199192517578 1102 76-77 0.7306139289060276 1102 78-79 0.7408874127061083 1102 80-81 0.7540403620289418 1102 82-83 0.784057699752033 1102 84-85 0.7382542952969828 1102 86-87 0.7023822366752697 1102 88-89 1.1137914606586037 1102 90-91 1.2537090926335068 1102 92-93 1.3716885775110619 1102 94-95 1.6007956284489673 1102 96-97 1.618929567355682 1102 98-99 1.8144839500221828 1102 100-101 2.2117341020161447 1103 1 0.3185004463492369 1103 2 0.31787744411225916 1103 3 0.4199815627684309 1103 4 0.33997400887478335 1103 5 0.28081370261180894 1103 6 0.18033113458601946 1103 7 0.19521053298381474 1103 8 0.22621187885398797 1103 9 0.26989108576450604 1103 10-11 0.41341261949557406 1103 12-13 0.4406413789050774 1103 14-15 0.614434891133925 1103 16-17 0.5091446170947762 1103 18-19 0.5280376062132248 1103 20-21 0.4011805807576678 1103 22-23 0.4954358032683075 1103 24-25 0.6289521167382688 1103 26-27 0.4781793156387195 1103 28-29 0.529706903854013 1103 30-31 0.4977608909374567 1103 32-33 0.37664890700919784 1103 34-35 0.6664802181959217 1103 36-37 0.6111079939213937 1103 38-39 0.6833882866239165 1103 40-41 0.7380800570207455 1103 42-43 0.8595721964422296 1103 44-45 0.8597873016003419 1103 46-47 0.8902031200641218 1103 48-49 0.9239359883776288 1103 50-51 0.7609949922312396 1103 52-53 0.7623245093203082 1103 54-55 0.8653543350748976 1103 56-57 0.9790667940496789 1103 58-59 1.0459000110414536 1103 60-61 1.1173627250178413 1103 62-63 1.1521255390981224 1103 64-65 1.2202745943979494 1103 66-67 1.1954916496102896 1103 68-69 1.2607985955718206 1103 70-71 1.325450230230878 1103 72-73 1.3384803556265688 1103 74-75 1.3739974857530584 1103 76-77 1.3743346149995936 1103 78-79 1.458493958702487 1103 80-81 1.3525197428176448 1103 82-83 1.4064231520962984 1103 84-85 1.3836221689487544 1103 86-87 1.3956418372441917 1103 88-89 1.297055230786782 1103 90-91 1.4559634061166342 1103 92-93 1.3985028649185693 1103 94-95 1.457045840101724 1103 96-97 1.843689447306243 1103 98-99 1.9575924305247305 1103 100-101 2.6590140466477834 1104 1 0.1313398926864231 1104 2 0.21134880005742573 1104 3 0.09137353411336235 1104 4 0.2701985504804938 1104 5 0.06164038686823403 1104 6 0.16519689817910432 1104 7 0.12622363329600006 1104 8 0.12577269636323507 1104 9 0.18575051108210516 1104 10-11 0.2125951620887605 1104 12-13 0.2954765979973999 1104 14-15 0.5672153219189511 1104 16-17 0.5024930563705681 1104 18-19 0.45676310873310655 1104 20-21 0.4425018345206553 1104 22-23 0.49530783256615507 1104 24-25 0.5332530338508334 1104 26-27 0.5220246700106586 1104 28-29 0.38959907152867856 1104 30-31 0.6588157453359571 1104 32-33 0.5287134146103298 1104 34-35 0.5838218358233576 1104 36-37 0.5428749874587098 1104 38-39 0.45268270476866945 1104 40-41 0.34466701683859213 1104 42-43 0.602994826327631 1104 44-45 0.7128523133088507 1104 46-47 0.8464769890299024 1104 48-49 0.9181578681927292 1104 50-51 0.8436673055916302 1104 52-53 0.8001335342677862 1104 54-55 0.9661064788271432 1104 56-57 0.938455696689573 1104 58-59 0.881105496351708 1104 60-61 0.7180759915947519 1104 62-63 0.909106907859794 1104 64-65 0.8635334217845809 1104 66-67 0.7739201953058767 1104 68-69 0.9044853958636665 1104 70-71 0.8223756936144113 1104 72-73 0.8212435119866832 1104 74-75 1.1138952517409706 1104 76-77 1.1075166729239925 1104 78-79 1.009853623114708 1104 80-81 0.9968681030431839 1104 82-83 1.085995853062137 1104 84-85 1.27607494290001 1104 86-87 1.5181230822468557 1104 88-89 1.4819845585615425 1104 90-91 1.5169872883654456 1104 92-93 1.6394671614166434 1104 94-95 1.7474735667848122 1104 96-97 2.216238403389287 1104 98-99 2.5311192123628707 1104 100-101 2.970461366131339 1105 1 0.3377498410223616 1105 2 0.15172229175856344 1105 3 0.3819984804754206 1105 4 0.30376131116087635 1105 5 0.24117815655979769 1105 6 0.3636555583462808 1105 7 0.33898431368253057 1105 8 0.4093680689109078 1105 9 0.4083977364987632 1105 10-11 0.3877200312121829 1105 12-13 0.2520569004295581 1105 14-15 0.40721420624980453 1105 16-17 0.578673091042134 1105 18-19 0.42402122308060797 1105 20-21 0.3781993851977816 1105 22-23 0.45251419702951523 1105 24-25 0.49128113154912256 1105 26-27 0.4369746365405831 1105 28-29 0.5044396166617844 1105 30-31 0.622041573934851 1105 32-33 0.6314176249740413 1105 34-35 0.5595826363588756 1105 36-37 0.6868799822236511 1105 38-39 0.6005410491156553 1105 40-41 0.6725608909567811 1105 42-43 0.9329393861532509 1105 44-45 0.7984653305931388 1105 46-47 1.0760958592861627 1105 48-49 1.0951986845192536 1105 50-51 0.7633219630812107 1105 52-53 0.8322240407815755 1105 54-55 0.8795425681493327 1105 56-57 0.9352254054141014 1105 58-59 0.9833870397512428 1105 60-61 0.9640617281938475 1105 62-63 0.8778432838231325 1105 64-65 0.9114368220008515 1105 66-67 0.9798671409470359 1105 68-69 0.9892882657079767 1105 70-71 0.9494439725660229 1105 72-73 1.2473149834684598 1105 74-75 1.1291915048090573 1105 76-77 1.3186437562215652 1105 78-79 1.5275043671802067 1105 80-81 1.1819936587322388 1105 82-83 1.2497464752622385 1105 84-85 1.2570042952969835 1105 86-87 1.4608300388730733 1105 88-89 1.4807066882881195 1105 90-91 1.5136188257575256 1105 92-93 1.5870731928956765 1105 94-95 1.8324184698932378 1105 96-97 2.0816552345456394 1105 98-99 2.3031515324397667 1105 100-101 2.3456724850616695 1106 1 0.1934215886295938 1106 2 0.31393933738574376 1106 3 0.2776687088645424 1106 4 0.31197521707063913 1106 5 0.3124370819661877 1106 6 0.25921944146776355 1106 7 0.1820465509844027 1106 8 -0.013068250530018588 1106 9 0.21446701284453695 1106 10-11 0.13901643066526503 1106 12-13 0.26596914026345786 1106 14-15 0.22801103614801832 1106 16-17 0.02105547824018572 1106 18-19 0.2605799408605307 1106 20-21 0.27379238666635786 1106 22-23 0.2828631107885684 1106 24-25 0.2018080470299637 1106 26-27 0.25771702778988725 1106 28-29 0.4348176487679254 1106 30-31 0.46809413888142615 1106 32-33 0.3580317942149378 1106 34-35 0.32877036107655755 1106 36-37 0.23556459254420048 1106 38-39 0.4003903933198387 1106 40-41 0.3188628608762727 1106 42-43 0.18969737470847292 1106 44-45 0.003899067363285269 1106 46-47 -0.03264344288631804 1106 48-49 0.2792000518155575 1106 50-51 0.010093624599413431 1106 52-53 -0.03148310460759518 1106 54-55 -0.05008724903809281 1106 56-57 0.14580929157402522 1106 58-59 0.23190960014016326 1106 60-61 0.16495933289328946 1106 62-63 0.5233666544617108 1106 64-65 0.4523265774067369 1106 66-67 0.5172158521436785 1106 68-69 0.3142287629987166 1106 70-71 0.6582288512817698 1106 72-73 0.8019840471236996 1106 74-75 0.7879890436757222 1106 76-77 0.8165763535781281 1106 78-79 1.1695917728620842 1106 80-81 1.2950386782288703 1106 82-83 0.9531355976605767 1106 84-85 1.1037164541059141 1106 86-87 1.2941161076430099 1106 88-89 1.3799648034448495 1106 90-91 1.4569393301379385 1106 92-93 1.6392798571991172 1106 94-95 1.8562790563114255 1106 96-97 1.856843870794183 1106 98-99 2.338910128682233 1106 100-101 2.442182081456057 1107 1 0.1788950594403076 1107 2 0.1979526847422015 1107 3 0.12986489977041416 1107 4 0.12487785695275022 1107 5 0.10650957838472408 1107 6 0.13029411459522322 1107 7 0.07471546564745069 1107 8 0.13142069161288106 1107 9 0.08931357390810746 1107 10-11 0.3136687578102624 1107 12-13 0.1688346032462178 1107 14-15 0.4135264005490171 1107 16-17 0.37603773938080565 1107 18-19 0.48122732866334417 1107 20-21 0.5191042595442212 1107 22-23 0.4640338462114997 1107 24-25 0.4937140882028572 1107 26-27 0.41165839434122375 1107 28-29 0.3370378625717194 1107 30-31 0.417622616269135 1107 32-33 0.36065527044545576 1107 34-35 0.5420080031998324 1107 36-37 0.6723960894929988 1107 38-39 0.6712317220790567 1107 40-41 0.6691328255157316 1107 42-43 0.7287565559963838 1107 44-45 0.6448812523337395 1107 46-47 0.786261991854893 1107 48-49 0.6590079272246001 1107 50-51 0.6412103085020178 1107 52-53 0.6711302644280579 1107 54-55 0.562490788759888 1107 56-57 0.6977148640074518 1107 58-59 0.7953747273882783 1107 60-61 0.8876786956419807 1107 62-63 1.2012620728263386 1107 64-65 1.2392998680457126 1107 66-67 1.10786933355962 1107 68-69 0.8305262485044054 1107 70-71 0.9134977759055403 1107 72-73 1.0192579755413256 1107 74-75 1.137274655424676 1107 76-77 1.298273542020187 1107 78-79 1.5195519300069904 1107 80-81 1.4568460790392024 1107 82-83 1.33028703859501 1107 84-85 1.3131140945885988 1107 86-87 1.5029197673264356 1107 88-89 1.649040302725627 1107 90-91 1.7056832548349412 1107 92-93 1.904280985103469 1107 94-95 1.9301963413724081 1107 96-97 2.078206086291292 1107 98-99 2.542673374234333 1107 100-101 2.6874544044409916 1108 1 0.10370577563501371 1108 2 0.03583235369378457 1108 3 0.10901137518554904 1108 4 0.1635084936159501 1108 5 0.20128070504187434 1108 6 0.2114218290336609 1108 7 0.12024637074416233 1108 8 0.10373297905406531 1108 9 0.20897647545703535 1108 10-11 0.11936283758846855 1108 12-13 0.15180052922436005 1108 14-15 0.41455505303628115 1108 16-17 0.43516359769255075 1108 18-19 0.16564981481581498 1108 20-21 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-1.226018422835164 2316 100-101 -1.1754394403714876 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4772.0 3 974.0 4 855.0 5 1040.0 6 1204.0 7 1213.0 8 1559.0 9 1880.0 10 2332.0 11 2785.0 12 3594.0 13 4559.0 14 5200.0 15 6128.0 16 7528.0 17 9741.0 18 8787.0 19 6843.0 20 6980.0 21 7240.0 22 8002.0 23 9060.0 24 10073.0 25 11610.0 26 13360.0 27 15480.0 28 18522.0 29 22296.0 30 27024.0 31 34102.0 32 43536.0 33 56508.0 34 76819.0 35 107451.0 36 155644.0 37 208472.0 38 158283.0 39 18128.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18330665621258 18.890467117468205 14.721404464477317 23.204821761841902 2 14.763529233658435 19.529259664136376 41.6230529392479 24.084158162957294 3 19.24852458407433 23.531897308265577 30.19404447374897 27.02553363391112 4 12.501636607259758 16.206915443351104 37.824431273173666 33.46701667621548 5 13.609955346904135 36.980915885229756 35.03332036483873 14.375808403027385 6 32.086103502781235 35.87475786638401 17.735457828607917 14.30368080222684 7 28.47278744574241 32.315575234872604 20.982757292582175 18.22888002680281 8 25.56108128578111 35.56132261106532 20.047912350656777 18.82968375249679 9 25.54103874984685 17.039796838974837 20.97641297527688 36.442751435901435 10-11 24.646049857937275 26.45346273835304 27.98166968490014 20.918817718809553 12-13 25.194480927827872 23.82357718672118 28.55381930747061 22.428122577980332 14-15 22.102107899623118 25.412207820775855 26.64694015543909 25.838744124161927 16-17 21.74736871415047 28.808867479720075 27.34875907259936 22.0950047335301 18-19 21.63688711056165 27.525090099747825 29.464589847756255 21.373432941934276 20-21 22.103857440091172 26.58122005950514 29.661808132121454 21.65311436828224 22-23 21.954835168455237 26.377222990626194 29.634137118427883 22.03380472249069 24-25 21.925162719999108 26.589431505412865 29.691690376367134 21.793715398220893 26-27 21.77101600054571 26.729370776370555 29.59634596742721 21.903267255656527 28-29 21.51309035622328 26.960119106183832 29.784574858125158 21.742215679467737 30-31 21.70000185625186 26.63730694980695 29.713858776358776 21.948832417582416 32-33 21.498891513078846 26.70917452183304 29.688096795017866 22.10383717007025 34-35 21.941478685750855 26.786218955827096 29.47254896228223 21.799753396139817 36-37 21.885712641822153 26.531855580364184 29.64734934325184 21.935082434561824 38-39 21.56284554131926 26.908455301830557 29.65798398521112 21.87071517163906 40-41 21.788126820835156 26.79271791212294 29.56969016745536 21.849465099586546 42-43 21.69099153780332 26.682972961434732 29.38613343133816 22.239902069423795 44-45 21.734851360368395 26.796013879776044 29.26418440539043 22.20495035446513 46-47 21.86489164000492 27.135681423238527 28.934589586953646 22.064837349802907 48-49 21.876035427995465 27.154038273595226 28.806917795950266 22.16300850245904 50-51 21.671503337558168 27.194543696881123 28.92372508897912 22.210227876581587 52-53 21.895549820841765 27.44527783450606 28.551928039656165 22.10724430499601 54-55 21.782939980881494 27.164799673314832 28.717435892676495 22.334824453127176 56-57 21.860108001781473 27.384621808194776 28.62673508016627 22.128535109857484 58-59 22.101137962134736 27.20582231311403 28.569446867263732 22.123592857487502 60-61 21.915546830813888 27.147020300180806 29.036454768962667 21.90097810004264 62-63 21.849497111103275 27.183051929612255 28.924974062106173 22.042476897178293 64-65 21.767159387207823 27.328916478764764 28.797659809407154 22.106264324620252 66-67 21.629844020700734 27.30375458291846 28.706816635936022 22.359584760444786 68-69 21.54054738764522 27.256027565189388 28.828052572822155 22.375372474343234 70-71 21.760951254604347 27.2786956439031 28.573456793273888 22.386896308218667 72-73 21.79424661843493 27.133530584529495 28.6853874352813 22.38683536175427 74-75 21.611325107340942 27.421840854236244 28.573847876983148 22.392986161439662 76-77 21.69722669644013 27.2888002862262 28.566655483055435 22.447317534278234 78-79 21.830338828569783 27.22819741270333 28.49590409664587 22.445559662081013 80-81 21.72101537540631 27.341449097823034 28.4900919461826 22.447443580588057 82-83 21.768186635632496 27.308769565725804 28.62164299197603 22.301400806665665 84-85 21.754452226089846 27.26796503734664 28.477032907173022 22.500549829390494 86-87 21.657453282714627 27.237683034227018 28.659130158806757 22.445733524251597 88-89 21.7999474728014 27.137165591529573 28.390765235903732 22.6721216997653 90-91 21.674228595133204 27.095337836771854 28.570122695344146 22.660310872750795 92-93 21.931111155449287 26.906627974904794 28.636621158683397 22.525639710962515 94-95 21.634459452875635 26.88966336126476 28.554784124976397 22.92109306088321 96-97 21.48196640384974 26.898958227976134 28.795898237236738 22.823177130937392 98-99 21.55920309645042 27.087491799064793 28.47332225337108 22.879982851113702 100-101 21.68339533317064 26.588465524904635 28.869546094381416 22.858593047543312 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1290.0 1 746.0 2 181.0 3 138.5 4 117.5 5 127.0 6 199.0 7 392.0 8 661.5 9 859.5 10 880.0 11 884.0 12 941.0 13 1029.0 14 1112.0 15 1163.5 16 1249.5 17 1305.0 18 1368.5 19 1500.5 20 1731.5 21 2079.5 22 2545.5 23 3132.0 24 3907.5 25 4957.0 26 6303.0 27 7910.5 28 9369.5 29 10806.5 30 12718.5 31 14807.0 32 16857.5 33 19210.0 34 22069.0 35 24520.0 36 27120.5 37 29696.5 38 31600.0 39 33174.5 40 34577.0 41 36646.0 42 38668.0 43 40399.5 44 42369.5 45 43892.0 46 43928.0 47 44596.5 48 47202.0 49 46159.0 50 42127.0 51 39244.5 52 36501.5 53 34703.0 54 32728.5 55 29631.5 56 26439.0 57 23398.0 58 20607.5 59 17596.0 60 14324.0 61 11123.0 62 8524.0 63 6552.0 64 4843.5 65 3585.0 66 2637.5 67 1940.0 68 1388.0 69 916.0 70 649.0 71 469.5 72 340.0 73 242.5 74 174.0 75 132.0 76 97.5 77 61.0 78 42.5 79 31.5 80 17.5 81 11.0 82 10.5 83 9.5 84 6.0 85 7.0 86 6.5 87 3.5 88 3.0 89 2.0 90 1.5 91 0.5 92 0.5 93 1.0 94 1.0 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2901114780216472 2 0.26843648253727126 3 0.25658007475094596 4 0.24657623068123402 5 0.24231533413302336 6 0.20229995785417546 7 0.19322239564277013 8 0.20387463701329678 9 0.2049861752432648 10-11 0.20785764900401543 12-13 0.2065608544023861 14-15 0.20243890013292146 16-17 0.2005400223233928 18-19 0.20081790688088477 20-21 0.1881278454220835 22-23 0.18104178920603753 24-25 0.18219964152892085 26-27 0.19363922247900814 28-29 0.2062829698448941 30-31 0.19868745860677944 32-33 0.20521774570784143 34-35 0.1990116572571868 36-37 0.18553425621882483 38-39 0.18696999309920018 40-41 0.18104178920603753 42-43 0.1933150238286008 44-45 0.18951726820954348 46-47 0.18919306955913615 48-49 0.19748329219098076 50-51 0.20614402756614808 52-53 0.2153605320562994 54-55 0.19405604931524614 56-57 0.1690464391409662 58-59 0.17312207931751553 60-61 0.17918922548942418 62-63 0.18627528170547014 64-65 0.17553041214911286 66-67 0.17988393688315418 68-69 0.18470060254634882 70-71 0.17918922548942418 72-73 0.17974499460440818 74-75 0.18006919325481552 76-77 0.1959549271247748 78-79 0.20072527869505413 80-81 0.19011935141744282 82-83 0.1910919473686648 84-85 0.18321855157305816 86-87 0.1839595770597035 88-89 0.18970252458120482 90-91 0.18877624272289814 92-93 0.1860437112408935 94-95 0.18576582668340147 96-97 0.17617880944992753 98-99 0.18192175697142882 100-101 0.1754377839632822 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1079585.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.56127068636472 #Duplication Level Percentage of deduplicated Percentage of total 1 82.1093902103033 54.65305347680343 2 11.029101912525055 14.682220756541659 3 3.090412126811222 6.171052743153176 4 1.2876646239618275 3.4283437435511694 5 0.6803482745148127 2.264242283049081 6 0.38665637782789736 1.544180389632719 7 0.2619366510858111 1.220438543492178 8 0.18507125037754596 0.9854862074115052 9 0.14817215692366675 0.8876274340660822 >10 0.7580585051504803 9.147640863395608 >50 0.046843673607511856 2.1091816971656283 >100 0.015086989784472156 1.6436478280175353 >500 4.190823754895949E-4 0.18912576384826715 >1k 8.381647509791898E-4 1.0737582698720693 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3290 0.3047467313828925 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2707 0.250744499043614 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1708 0.15820894139877822 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1422 0.13171728025120766 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1410 0.13060574202123965 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.631409291533321E-5 0.0 24-25 0.0 0.0 0.0 9.262818583066641E-5 0.0 26-27 0.0 0.0 0.0 3.7051274332266566E-4 0.0 28-29 0.0 0.0 0.0 0.0011578523228833301 0.0 30-31 0.0 0.0 0.0 0.004260896548210655 0.0 32-33 0.0 0.0 0.0 0.008892305839743975 0.0 34-35 0.0 0.0 0.0 0.011856407786325301 0.0 36-37 0.0 0.0 0.0 0.016163618427451287 0.0 38-39 0.0 0.0 0.0 0.0249632960813646 0.0 40-41 0.0 0.0 0.0 0.03399454419985457 0.0 42-43 0.0 0.0 0.0 0.04247002320336055 0.0 44-45 0.0 0.0 0.0 0.047981400260285204 0.0 46-47 0.0 0.0 0.0 0.05516008466216185 0.0 48-49 0.0 0.0 0.0 0.06303348045776849 0.0 50-51 0.0 0.0 0.0 0.07312995271331113 0.0 52-53 0.0 0.0 0.0 0.09105350667154508 0.0 54-55 0.0 0.0 0.0 0.10541087547529837 0.0 56-57 0.0 0.0 0.0 0.14579676449746892 0.0 58-59 0.0 0.0 0.0 0.21202591736639542 0.0 60-61 0.0 0.0 0.0 0.2685291107231019 0.0 62-63 0.0 0.0 0.0 0.3035888790600092 0.0 64-65 0.0 0.0 0.0 0.36310248845621235 0.0 66-67 0.0 0.0 0.0 0.4637893264541467 0.0 68-69 0.0 0.0 0.0 0.6055104507750664 0.0 70-71 0.0 0.0 0.0 0.7452863831935419 0.0 72-73 0.0 0.0 0.0 0.9026616709198443 0.0 74-75 0.0 0.0 0.0 1.091391599549827 0.0 76-77 0.0 0.0 0.0 1.2315843588045405 0.0 78-79 0.0 0.0 0.0 1.3844671795180554 0.0 80-81 0.0 0.0 0.0 1.5512442281061705 0.0 82-83 0.0 0.0 0.0 1.7229305705433107 0.0 84-85 0.0 0.0 0.0 1.9269904639282687 0.0 86-87 0.0 0.0 0.0 2.2203902425469044 0.0 88-89 0.0 0.0 0.0 2.6368928801344964 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3180 0.0 28.411898 1 GTACATG 3295 0.0 26.265747 1 GTATCAA 4310 0.0 25.376068 1 TACATGG 3405 0.0 24.15022 2 CGACTTA 50 0.0016542026 23.749779 14-15 ACATGGG 3420 0.0 23.203926 3 GGACCGC 110 0.0028758547 21.592714 6 TCAACGC 5180 0.0 20.734335 4 ATCAACG 5240 0.0 20.585701 3 CAACGCA 5255 0.0 20.438412 5 GGACCGA 140 4.8487308E-4 20.358845 6 TATAGGG 235 1.123517E-7 20.226664 2 TATCAAC 5395 0.0 20.087946 2 AACGCAG 5380 0.0 19.9552 6 GAGTACT 3170 0.0 19.104712 12-13 AGAGTAC 4960 0.0 18.914074 10-11 AGTACTT 3320 0.0 18.670761 12-13 CTTTGCG 155 9.5796556E-4 18.39974 2 CATGGGG 1860 0.0 18.39632 4 TAGACTG 395 1.8189894E-11 18.047022 5 >>END_MODULE