FastQCFastQC Report
Thu 26 May 2016
SRR937677_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937677_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1079585
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43570.4035810056642135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39660.367363385004423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25040.2319409773199887No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT19990.18516374347550216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58600.026.3577231
GGTATCA44000.025.274661
GTACATG33100.023.6907121
GCACCGC2052.671004E-823.168976
GAGTACT39950.022.11858612-13
TCAACGC69200.021.9656814
ATCAACG69300.021.9339853
CAACGCA69500.021.8708675
TACATGG35050.021.6836682
AACGCAG70700.021.5648386
TATCAAC70950.021.5577932
ACATGGG33650.021.033043
GTACTTT41650.020.81174314-15
AGTACTT42000.020.3603112-13
TGGACCG1908.249308E-620.000335
AGAGTAC59200.019.90174510-11
CTATACC2651.8540959E-819.7173084
CTACGCA1456.1450206E-419.6554994
ACGCAGA79200.019.2548897
ATTCGCG757.7280117E-418.99855680-81
Produced by FastQC (version 0.11.5)