##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937677_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1079585 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49670845741651 34.0 31.0 34.0 31.0 34.0 2 32.7180564754049 34.0 31.0 34.0 31.0 34.0 3 32.782805429864254 34.0 31.0 34.0 31.0 34.0 4 36.304850474951024 37.0 37.0 37.0 35.0 37.0 5 36.249676496060985 37.0 37.0 37.0 35.0 37.0 6 36.298268316065894 37.0 37.0 37.0 35.0 37.0 7 36.26285563434097 37.0 37.0 37.0 35.0 37.0 8 36.23417331659851 37.0 37.0 37.0 35.0 37.0 9 37.983942903986254 39.0 38.0 39.0 35.0 39.0 10-11 37.909650004399836 39.0 38.0 39.0 35.0 39.0 12-13 37.822058476173716 39.0 38.0 39.0 35.0 39.0 14-15 39.14352505823997 40.0 38.0 41.0 36.0 41.0 16-17 39.11010712449691 40.0 38.0 41.0 36.0 41.0 18-19 39.06314324485798 40.0 38.0 41.0 36.0 41.0 20-21 38.95870079706554 40.0 38.0 41.0 35.5 41.0 22-23 38.91543972915518 40.0 38.0 41.0 35.0 41.0 24-25 38.93556505509061 40.0 38.0 41.0 35.0 41.0 26-27 38.77825414395346 40.0 38.0 41.0 35.0 41.0 28-29 38.76497959864207 40.0 38.0 41.0 35.0 41.0 30-31 38.802142026797334 40.0 38.0 41.0 35.0 41.0 32-33 38.70302708911295 40.0 38.0 41.0 35.0 41.0 34-35 38.57854731216162 40.0 38.0 41.0 35.0 41.0 36-37 38.43235039390136 40.0 38.0 41.0 34.5 41.0 38-39 38.31131175405364 40.0 38.0 41.0 34.0 41.0 40-41 38.15502299494713 40.0 38.0 41.0 34.0 41.0 42-43 37.96469800895714 40.0 38.0 41.0 33.5 41.0 44-45 37.68667034091804 40.0 37.5 41.0 33.0 41.0 46-47 37.37632006743332 40.0 37.0 41.0 33.0 41.0 48-49 37.06301588110246 40.0 37.0 41.0 32.0 41.0 50-51 36.835213067984455 40.0 37.0 41.0 31.0 41.0 52-53 36.601840985193384 39.5 36.0 41.0 31.0 41.0 54-55 36.38924772018878 39.0 36.0 41.0 31.0 41.0 56-57 36.136668719924785 39.0 35.5 40.0 30.0 41.0 58-59 35.8949035972156 39.0 35.0 40.0 29.5 41.0 60-61 35.5634026037783 38.0 35.0 40.0 29.0 41.0 62-63 35.17336430202346 38.0 34.5 40.0 28.0 41.0 64-65 34.80906413112446 37.5 34.0 40.0 28.0 41.0 66-67 34.398119184686706 37.0 34.0 39.5 26.5 41.0 68-69 33.93484764979135 36.0 33.5 39.0 26.0 40.0 70-71 33.515501326898764 36.0 33.0 39.0 26.0 40.0 72-73 33.03692298429489 35.0 32.5 38.0 25.5 40.0 74-75 32.55152026009995 35.0 32.0 37.0 24.5 39.0 76-77 31.29757499409495 34.0 30.5 36.0 23.0 38.5 78-79 31.886590680678225 35.0 32.0 36.0 24.0 39.0 80-81 31.69885326305942 35.0 32.0 36.0 24.0 37.5 82-83 31.309894079669505 34.0 31.5 35.5 22.5 37.0 84-85 30.90989037454207 34.0 31.0 35.0 20.5 37.0 86-87 30.58712236646489 34.0 31.0 35.0 19.5 36.0 88-89 30.200873483792385 34.0 31.0 35.0 18.0 36.0 90-91 29.72292454971123 34.0 30.0 35.0 10.5 35.5 92-93 29.292221548094872 34.0 29.5 35.0 4.5 35.0 94-95 28.820259636804884 34.0 29.0 35.0 2.0 35.0 96-97 28.34675824506639 34.0 29.0 35.0 2.0 35.0 98-99 27.55916625369934 33.0 28.0 35.0 2.0 35.0 100-101 25.082562744017377 31.0 22.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.12875210814859628 1101 2 0.12162797873444475 1101 3 0.09075691285747212 1101 4 0.08203352597409719 1101 5 0.02878845105711747 1101 6 0.0239381804959109 1101 7 0.09627499392112071 1101 8 0.05576017649386955 1101 9 0.07466166402054597 1101 10-11 0.04299969713713381 1101 12-13 0.024545697770903985 1101 14-15 0.14666077350270967 1101 16-17 0.13486433109170548 1101 18-19 0.1741340529387685 1101 20-21 0.09854684356301391 1101 22-23 -0.038721962144499855 1101 24-25 -0.11127377171174402 1101 26-27 -0.13866775158340516 1101 28-29 -0.019948252466015504 1101 30-31 -0.06520422456758723 1101 32-33 0.04972060217389185 1101 34-35 -0.10569139941328842 1101 36-37 -0.03048117158154895 1101 38-39 0.0205034189722042 1101 40-41 0.0014086289444037448 1101 42-43 -0.05499431355692508 1101 44-45 -0.09666844924774409 1101 46-47 -0.08281774502864181 1101 48-49 -0.18183811595918087 1101 50-51 -0.24746342354684003 1101 52-53 -0.2871209023302512 1101 54-55 -0.3005953371813419 1101 56-57 -0.24438160819720878 1101 58-59 -0.25068508389297506 1101 60-61 -0.19170257133665558 1101 62-63 -0.2647018760504949 1101 64-65 -0.18838753661894003 1101 66-67 -0.2764503426645817 1101 68-69 -0.19465433448945646 1101 70-71 -0.4601230636687248 1101 72-73 -0.40980547420270597 1101 74-75 -0.1792337346628372 1101 76-77 -0.4067163576772792 1101 78-79 -0.364642357765554 1101 80-81 -0.18864793001057123 1101 82-83 -0.12171455399289499 1101 84-85 -0.227121690407472 1101 86-87 -0.12081100280531487 1101 88-89 0.04383043052919433 1101 90-91 0.03364055451069703 1101 92-93 0.24160223364289024 1101 94-95 0.2274637008632574 1101 96-97 0.10349220864014086 1101 98-99 -0.1356823603824715 1101 100-101 0.48657705278332486 1102 1 0.11354245746408509 1102 2 0.05829919857605148 1102 3 0.09488162892353103 1102 4 0.025875996352880293 1102 5 0.034144826525221106 1102 6 0.09042860348075266 1102 7 0.01236100288422648 1102 8 0.055460894770007485 1102 9 0.16014496563513347 1102 10-11 0.15107685043076202 1102 12-13 0.02578119411813873 1102 14-15 0.028751448191151496 1102 16-17 0.09987522801601045 1102 18-19 0.22488148985939205 1102 20-21 0.16354085792853112 1102 22-23 0.04852249730667069 1102 24-25 0.11077983808628034 1102 26-27 0.033820200408740675 1102 28-29 0.044426095253307096 1102 30-31 0.05787730284355774 1102 32-33 0.09551262439747887 1102 34-35 0.049236772972982124 1102 36-37 0.016979921794352038 1102 38-39 7.489067246737591E-4 1102 40-41 -0.0799480381315405 1102 42-43 -0.03981343996124309 1102 44-45 -0.09237298912128722 1102 46-47 -0.1420409938468623 1102 48-49 -0.2295409368812784 1102 50-51 -0.28284581350325766 1102 52-53 -0.333358010194452 1102 54-55 -0.2963248171985313 1102 56-57 -0.22840456847715274 1102 58-59 -0.19893812275356026 1102 60-61 -0.1659336475230404 1102 62-63 -0.2179275958614113 1102 64-65 -0.13563913278813544 1102 66-67 -0.17064082397097735 1102 68-69 0.032356608478188775 1102 70-71 -0.18207691289212846 1102 72-73 -0.13127970527889232 1102 74-75 -0.10119602516562765 1102 76-77 -0.29592264719780914 1102 78-79 -0.3576399328162019 1102 80-81 -0.36128168591946874 1102 82-83 -0.2712479814592861 1102 84-85 -0.14228491405533106 1102 86-87 -0.09683585086228774 1102 88-89 -0.05045098637792478 1102 90-91 -0.04346094036314696 1102 92-93 0.12467324782423717 1102 94-95 0.05688654756962208 1102 96-97 -0.021461748017724602 1102 98-99 0.005649596766527765 1102 100-101 0.5747941778370418 1103 1 0.07711701849627417 1103 2 0.0832900975076285 1103 3 0.06967484418284187 1103 4 -0.05283554634171139 1103 5 -0.008482038135539938 1103 6 0.07797813022519051 1103 7 0.06597052895138233 1103 8 0.12504597071101387 1103 9 0.07465736002041012 1103 10-11 0.02921936469616071 1103 12-13 0.07962882953356853 1103 14-15 0.09079216171491566 1103 16-17 0.09257214973835914 1103 18-19 0.22963521071481097 1103 20-21 0.1737075979681535 1103 22-23 0.24695012899700686 1103 24-25 -0.035662173292173804 1103 26-27 0.0373373400487651 1103 28-29 0.019673834801892554 1103 30-31 0.12567602954057833 1103 32-33 0.17564488620346452 1103 34-35 0.23498957948849863 1103 36-37 0.16280179060350264 1103 38-39 0.1948159625502086 1103 40-41 0.09979753409284342 1103 42-43 0.18566923414430647 1103 44-45 0.16270297305213433 1103 46-47 0.08759483642456445 1103 48-49 0.17088437932793 1103 50-51 0.2556903325540745 1103 52-53 0.3867698492342484 1103 54-55 0.38375252071779187 1103 56-57 0.4013174044651535 1103 58-59 0.253904056253873 1103 60-61 0.31983376303251276 1103 62-63 0.17588463004827304 1103 64-65 0.2374141910067138 1103 66-67 0.2998574277772761 1103 68-69 0.16449618500335106 1103 70-71 0.06886652925894055 1103 72-73 0.2810172139036169 1103 74-75 0.17283132460324424 1103 76-77 0.18720069132806927 1103 78-79 0.22443053908477495 1103 80-81 0.4410880841409828 1103 82-83 0.5229493466363486 1103 84-85 0.40571105184318057 1103 86-87 0.5010223290933489 1103 88-89 0.6160467108401129 1103 90-91 0.2801354843985351 1103 92-93 0.44034751000273786 1103 94-95 0.40072015040746223 1103 96-97 0.4649141642056449 1103 98-99 0.6490046527275162 1103 100-101 1.402713610099692 1104 1 0.0683826131032319 1104 2 0.08249246746024141 1104 3 0.0381441590763032 1104 4 0.01201611779577405 1104 5 0.021039344958850847 1104 6 0.08095936162084172 1104 7 0.09779732854342882 1104 8 0.08617397899444512 1104 9 0.0666130244896479 1104 10-11 0.13397034990858003 1104 12-13 0.10105857653387318 1104 14-15 0.062162685818115904 1104 16-17 0.15804293289313165 1104 18-19 0.1470157055175818 1104 20-21 0.15677496822529946 1104 22-23 0.08010030763762899 1104 24-25 -0.04542164020833894 1104 26-27 -0.10760584095947934 1104 28-29 0.0644658483933469 1104 30-31 0.04079419671502649 1104 32-33 -0.01094255309953951 1104 34-35 -0.05056197747757807 1104 36-37 -0.13854167498565317 1104 38-39 -0.020311535146312565 1104 40-41 -0.06641308170865301 1104 42-43 0.04089808463694311 1104 44-45 0.09555301989912834 1104 46-47 0.03277866714815758 1104 48-49 0.14400081258035868 1104 50-51 0.1315691187049879 1104 52-53 0.2998239623746102 1104 54-55 0.05667463288880725 1104 56-57 -0.07514991732511334 1104 58-59 -0.055808045303670895 1104 60-61 -0.3067765619677161 1104 62-63 -0.30589946944618873 1104 64-65 -0.26943629126464685 1104 66-67 -0.10017315560066464 1104 68-69 -0.14653223291590933 1104 70-71 -0.19300267237234436 1104 72-73 0.026568045162420617 1104 74-75 0.06740679232806457 1104 76-77 -0.13900893958498628 1104 78-79 -0.08250799998872793 1104 80-81 0.11157648513571772 1104 82-83 -0.041670998307868246 1104 84-85 -0.001544530595225524 1104 86-87 -0.04154623404512847 1104 88-89 -0.09413496220025408 1104 90-91 0.08736657421647465 1104 92-93 0.28335337656849546 1104 94-95 0.21176638703209605 1104 96-97 0.21225690927116148 1104 98-99 0.3969474021991104 1104 100-101 0.6405431103595234 1105 1 0.22508091900254357 1105 2 0.1335935470846863 1105 3 0.08010533379009388 1105 4 0.11885087861347898 1105 5 0.09897999136038749 1105 6 -0.009316294478431075 1105 7 0.06467654448547933 1105 8 0.08352211925979702 1105 9 0.10027447897893182 1105 10-11 0.09245243912778278 1105 12-13 0.12713519725785716 1105 14-15 0.18065490188030964 1105 16-17 0.2518179281730042 1105 18-19 0.12645134857211104 1105 20-21 0.18267351098975837 1105 22-23 0.16694871394717126 1105 24-25 0.12843093698737817 1105 26-27 0.028492492402463654 1105 28-29 -0.0018160554531263529 1105 30-31 -0.02104734393824259 1105 32-33 0.02360328373813303 1105 34-35 0.08035922195257683 1105 36-37 0.10741359526374339 1105 38-39 0.08005620656768997 1105 40-41 0.053185792323716896 1105 42-43 0.06963514716591845 1105 44-45 0.10541939706396164 1105 46-47 0.18501944571357853 1105 48-49 0.1640717004813581 1105 50-51 0.2635729463083578 1105 52-53 0.2437122409829371 1105 54-55 0.2508298138846712 1105 56-57 0.2866268286971021 1105 58-59 0.21133464019777648 1105 60-61 0.19856792233567688 1105 62-63 0.004315339774386473 1105 64-65 0.2611720917016598 1105 66-67 0.3257386893728196 1105 68-69 0.3730061375205693 1105 70-71 0.23955770249248332 1105 72-73 0.21633607180116599 1105 74-75 0.3604111176915197 1105 76-77 0.33789799644425855 1105 78-79 0.1301544156924308 1105 80-81 0.1361967285232346 1105 82-83 0.17529832937274037 1105 84-85 0.19736383005770008 1105 86-87 0.3317257660921875 1105 88-89 0.3333598456471947 1105 90-91 0.5580206137969803 1105 92-93 0.5191689307127767 1105 94-95 0.5326027956072998 1105 96-97 0.611897749627488 1105 98-99 0.6521468495137803 1105 100-101 0.8400316189673376 1106 1 -0.1938839870591309 1106 2 -0.12282008536580946 1106 3 -0.03886970039231841 1106 4 0.00589194814302374 1106 5 0.06941600695769523 1106 6 0.11145394910287365 1106 7 0.1301826973188227 1106 8 0.0890925856597633 1106 9 -0.10029016375160893 1106 10-11 -0.05070221727572033 1106 12-13 -0.019038905137449547 1106 14-15 -0.10384780739197907 1106 16-17 -0.017791379569949584 1106 18-19 0.003949196346454187 1106 20-21 0.05961051407883389 1106 22-23 0.06050849517976786 1106 24-25 -0.11618976489138078 1106 26-27 0.025766761911754088 1106 28-29 0.05447350751845903 1106 30-31 0.025397685686513682 1106 32-33 -0.11107413561670398 1106 34-35 -0.1697015467717904 1106 36-37 -0.08319510479196168 1106 38-39 0.06503240548398281 1106 40-41 0.2545632167426177 1106 42-43 -0.05994149738769039 1106 44-45 -0.003054041939435592 1106 46-47 0.18565751731939173 1106 48-49 -0.2576227341163033 1106 50-51 -0.22334785178755823 1106 52-53 -0.5218660747105801 1106 54-55 -0.5154625343201147 1106 56-57 -0.2540160537660583 1106 58-59 -0.3044525679857415 1106 60-61 -0.33181010977046554 1106 62-63 -0.2516589854395761 1106 64-65 -0.6074244039685723 1106 66-67 -0.39667990585683555 1106 68-69 -0.1622663089269878 1106 70-71 -0.13722358074041097 1106 72-73 0.011101099400299574 1106 74-75 -0.07816562814329586 1106 76-77 -0.21883340579405441 1106 78-79 -0.3077462780838367 1106 80-81 -0.3987351598648736 1106 82-83 -0.49386407504312047 1106 84-85 -0.1797162327366486 1106 86-87 0.24429982797500216 1106 88-89 0.20028013733707084 1106 90-91 0.01909444779708025 1106 92-93 0.2820088557646834 1106 94-95 0.5798504787996848 1106 96-97 0.29425076527543226 1106 98-99 -0.10925868044013853 1106 100-101 0.7021980239908885 1107 1 0.11669839243820235 1107 2 0.09112432799209103 1107 3 0.0711156222029743 1107 4 0.0851689761004053 1107 5 0.05848412359178923 1107 6 0.04430994940754829 1107 7 0.04592970690747933 1107 8 0.08748568815843072 1107 9 0.04641210758408931 1107 10-11 0.03550776042985859 1107 12-13 0.0578570727849268 1107 14-15 0.10715599818660593 1107 16-17 0.18446677283483126 1107 18-19 0.17779221549739077 1107 20-21 0.09617072805840365 1107 22-23 0.14841692106472948 1107 24-25 -0.026557256637175897 1107 26-27 0.10472372869863733 1107 28-29 0.19785505265953418 1107 30-31 0.10284370008267985 1107 32-33 0.23644271703211217 1107 34-35 0.17659804806153545 1107 36-37 0.29380253100332965 1107 38-39 0.25702955789169124 1107 40-41 0.23971809119368004 1107 42-43 0.2279670749783591 1107 44-45 0.16151233010494082 1107 46-47 0.004499965194092681 1107 48-49 0.01596072055220077 1107 50-51 9.527742726049837E-4 1107 52-53 0.21961458539177414 1107 54-55 0.24980518915874939 1107 56-57 0.13368788452438451 1107 58-59 0.12675039327587 1107 60-61 0.23645373781889845 1107 62-63 0.1823681311388654 1107 64-65 0.5131580927136312 1107 66-67 0.39962130026841436 1107 68-69 0.2874225652486899 1107 70-71 0.2445163801784318 1107 72-73 0.11114548316901818 1107 74-75 0.35914193233141845 1107 76-77 0.013952137108791618 1107 78-79 0.18381860864234412 1107 80-81 0.3547664704696025 1107 82-83 0.48004617892578594 1107 84-85 0.4125097231256696 1107 86-87 0.5328895373858273 1107 88-89 0.6521361991638095 1107 90-91 0.701978279507891 1107 92-93 0.6641933867761942 1107 94-95 0.8276356847960358 1107 96-97 0.9238770041392073 1107 98-99 1.042081914448847 1107 100-101 1.494087952244449 1108 1 0.09284922035293874 1108 2 0.07881133569267007 1108 3 0.11710909051666363 1108 4 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84-85 0.5171806204102047 2315 86-87 0.118719427192989 2315 88-89 0.4765532593763204 2315 90-91 0.28891501701281186 2315 92-93 0.5352210333720215 2315 94-95 0.586502764685843 2315 96-97 0.7265506562446795 2315 98-99 1.211183646050575 2315 100-101 1.652809912102775 2316 1 -0.4624749873378917 2316 2 -0.4779147026240338 2316 3 -0.4679421725052464 2316 4 -0.21320835391931325 2316 5 -0.22038333408028876 2316 6 -0.10805534811093764 2316 7 -0.19110013082552513 2316 8 -0.31000214127314507 2316 9 -0.3546828630739114 2316 10-11 -0.4697257470638192 2316 12-13 -0.4427430302762545 2316 14-15 -0.5075408736674731 2316 16-17 -0.4468318632352819 2316 18-19 -0.48268534477926295 2316 20-21 -0.5033724543577094 2316 22-23 -0.29319805194150916 2316 24-25 -0.23037595593553561 2316 26-27 -0.6223728813713549 2316 28-29 -0.5066061482858473 2316 30-31 -0.5310390754374694 2316 32-33 -0.4009363111053261 2316 34-35 -0.5658316300788471 2316 36-37 -0.8462715251932806 2316 38-39 -1.0080474879487866 2316 40-41 -0.7417798182299009 2316 42-43 -0.8100111865056832 2316 44-45 -1.0549998535092016 2316 46-47 -0.9332915250835825 2316 48-49 -0.737756765711751 2316 50-51 -0.7601322816391729 2316 52-53 -0.7942899764786304 2316 54-55 -0.6929904213917624 2316 56-57 -0.6411299646279645 2316 58-59 -0.6828473419873617 2316 60-61 -0.7357773627396753 2316 62-63 -0.3990925979863533 2316 64-65 -0.5909880541246295 2316 66-67 -0.3834675345532119 2316 68-69 -0.28280906148720675 2316 70-71 -0.407482550072622 2316 72-73 -0.4589382920880354 2316 74-75 -0.25606480969602075 2316 76-77 -0.24623493849767186 2316 78-79 -0.2334519660868004 2316 80-81 -0.1746537995332922 2316 82-83 -0.1975528000292961 2316 84-85 -0.2817845143629434 2316 86-87 -0.1754114534365101 2316 88-89 0.0748968471881426 2316 90-91 -0.3487304291616411 2316 92-93 -0.15204353217292876 2316 94-95 -0.21469114959509028 2316 96-97 -0.02354874941411822 2316 98-99 -0.21635486056251452 2316 100-101 0.39308908504769136 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 5.0 4 7.0 5 19.0 6 34.0 7 71.0 8 173.0 9 280.0 10 574.0 11 955.0 12 1572.0 13 2531.0 14 3160.0 15 4513.0 16 6271.0 17 9694.0 18 8868.0 19 5117.0 20 4160.0 21 4273.0 22 4325.0 23 4634.0 24 5358.0 25 6299.0 26 7617.0 27 9657.0 28 11974.0 29 15298.0 30 19454.0 31 25221.0 32 33116.0 33 45677.0 34 65406.0 35 99448.0 36 162843.0 37 266025.0 38 224514.0 39 20437.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.89576012336344 19.651872692495886 14.989416989161217 22.462950194979463 2 15.412218583992923 20.35819319460719 40.628945381790224 23.600642839609666 3 19.71526095675653 24.009503651866225 30.334897205870774 25.940338185506466 4 13.194236674277615 17.043215680099298 37.19095763649921 32.571590009123874 5 13.668352322045093 37.57337881113589 34.532992558598835 14.225276308220186 6 32.32251281742521 36.534686939888935 17.025801581163133 14.116998661522715 7 28.662773195255586 32.95766428766609 20.421736130087023 17.957826386991297 8 25.976092665237104 36.193259446917104 19.293246942112017 18.53740094573378 9 25.954880810681885 17.35361273081786 20.567718150956154 36.1237883075441 10-11 25.022994947132464 27.0806837812678 27.224859552513237 20.6714617190865 12-13 25.737523447812695 24.20393228503277 28.17327065144392 21.885273615710613 14-15 22.411019048986418 25.852295094874417 26.407276870278857 25.329408985860308 16-17 21.811158917547022 29.243737176785523 27.134037616306266 21.81106628936119 18-19 21.61404613809936 27.749320340686467 29.595075885641243 21.04155763557293 20-21 22.26922611998468 26.774822975056487 29.87413154844085 21.08181935651798 22-23 22.11564157346199 26.684411410670094 29.74336505742804 21.456581958439877 24-25 22.157634646646628 26.767368942695573 29.892088163507275 21.182908247150525 26-27 21.908389875645508 26.933376560220456 29.843781117569414 21.31445244656462 28-29 21.527241940518746 27.29279424065389 30.042646727808464 21.137317091018897 30-31 21.861826535196393 26.86939888938805 29.89343127220182 21.375343303213732 32-33 21.75224739135872 27.02682049120727 29.738418003214196 21.482514114219818 34-35 22.15156750047472 26.915574040024637 29.70178355571817 21.23107490378247 36-37 22.15886433770864 26.67385463719043 29.75565068501081 21.411630340090117 38-39 21.856870927254455 27.097495796996068 29.78246270557668 21.2631705701728 40-41 21.977523718659757 27.173791720410435 29.62432236535883 21.224362195570972 42-43 21.97949214452317 27.08741664485555 29.336054125544024 21.597037085077258 44-45 22.089969756897325 27.07206935998555 29.217708656567105 21.620252226550015 46-47 22.234099214049845 27.598290083689562 28.704085366136063 21.46352533612453 48-49 22.351922266426453 27.67980288722055 28.490114256867223 21.478160589485775 50-51 22.0672295372759 27.688509936688632 28.573618566393566 21.670641959641898 52-53 22.30362699723829 28.023095922551683 28.07848760333258 21.594789476877445 54-55 22.113636258377063 27.851165031007284 28.223067197117413 21.812131513498244 56-57 22.2037634831903 27.98056660661273 28.29406670155662 21.521603208640357 58-59 22.502396754308368 27.714816341464545 28.152808718164852 21.62997818606224 60-61 22.310193268709735 27.730099992126604 28.614189711787404 21.34551702737626 62-63 22.156430480230828 27.770856393892096 28.663236336184738 21.409476789692334 64-65 22.12336221788928 27.882797556468457 28.353117170023665 21.640723055618594 66-67 22.014060958609093 27.97311003765336 28.302125353723884 21.710703650013663 68-69 21.957696707531134 27.884974318835475 28.360110598053883 21.797218375579504 70-71 22.213535756795437 27.901091623170014 28.13067058175132 21.75470203828323 72-73 22.179958039431817 27.762936684003574 28.275216865740077 21.78188841082453 74-75 22.123315903796367 28.14803836659457 28.035541434903227 21.693104294705837 76-77 22.095897960790488 27.967830231061008 28.13752506750279 21.79874674064571 78-79 22.150641218616414 27.957733758805464 27.89669178434306 21.994933238235063 80-81 22.202049861752432 28.082318668747714 27.8959044447635 21.81972702473636 82-83 22.20148890821734 28.111224426468368 27.925365627283867 21.761921038030422 84-85 22.025031887312046 28.12238614353876 27.847928320471887 22.004653648677312 86-87 21.979047504365106 28.112885970071833 28.015487432670888 21.892579092892177 88-89 22.092239147450176 28.097972832153097 27.68915833398945 22.120629686407277 90-91 22.160766568824492 27.943475415242535 27.845139164554112 22.05061885137886 92-93 22.35409252339392 27.714871141230546 27.83098390631687 22.100052429058668 94-95 22.006882274207218 27.812446449330068 27.818652537780718 22.362018738681993 96-97 21.854369966237027 27.939439692103914 28.01659897090086 22.189591370758208 98-99 21.94534435925165 28.152541156146732 27.57032264936978 22.331791835231837 100-101 21.532988551801765 28.569913217886334 27.540339670797714 22.356758559514187 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 132.0 1 100.0 2 80.0 3 108.5 4 107.5 5 116.0 6 213.5 7 471.0 8 794.5 9 1039.0 10 1104.5 11 1076.0 12 1205.5 13 1366.0 14 1480.5 15 1583.5 16 1705.5 17 1808.5 18 1841.5 19 1955.5 20 2129.5 21 2376.0 22 2858.0 23 3411.5 24 4123.5 25 5211.5 26 6481.0 27 8080.0 28 9517.5 29 11005.0 30 13060.0 31 14997.0 32 16746.5 33 19220.5 34 22137.5 35 24427.5 36 27112.5 37 29597.0 38 31538.0 39 33102.0 40 34172.0 41 36425.5 42 38421.0 43 39898.0 44 42025.0 45 43380.5 46 43494.5 47 44750.0 48 47328.5 49 45762.0 50 42078.0 51 39452.0 52 36592.0 53 34240.5 54 31868.0 55 29070.0 56 26046.0 57 23209.5 58 20749.0 59 17872.5 60 14400.5 61 11138.5 62 8425.5 63 6381.5 64 4811.5 65 3595.5 66 2564.0 67 1946.0 68 1362.0 69 809.5 70 575.5 71 392.5 72 291.0 73 214.5 74 158.0 75 127.5 76 78.5 77 44.0 78 26.0 79 14.0 80 11.0 81 7.5 82 5.0 83 4.5 84 4.0 85 2.5 86 2.0 87 1.5 88 0.5 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0460362083578412 2 0.0 3 0.0 4 0.0 5 0.007780767609775979 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.005557691149839985 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.094550220686653E-4 22-23 0.001528365066205996 24-25 0.0 26-27 9.262818583066642E-4 28-29 0.0016673073449519956 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.2419865040733247E-4 38-39 0.0 40-41 2.3157046457666604E-4 42-43 0.022462335063936606 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 4.631409291533321E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.8525637166133283E-4 84-85 1.8525637166133283E-4 86-87 0.0 88-89 0.0 90-91 0.011624837321748635 92-93 0.002871473760750659 94-95 0.0 96-97 0.0 98-99 6.947113937299981E-4 100-101 0.01458893926832996 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1079585.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.90770199868179 #Duplication Level Percentage of deduplicated Percentage of total 1 79.95608844379485 50.298537848024914 2 11.976887724297768 15.068769676635881 3 3.5919822707344964 6.778900508157422 4 1.5762529303755226 3.966337984744492 5 0.7822867488751084 2.4605930837876464 6 0.477975338584714 1.8040998097443732 7 0.3098259818660247 1.364330837707381 8 0.21956915276654426 1.105007266427264 9 0.17272667286556254 0.9779254257465542 >10 0.8571204055940532 9.758171667887236 >50 0.054260718080388626 2.3009463080531187 >100 0.023534112690312387 2.548762035763461 >500 8.936996848112845E-4 0.36580866137107965 >1k 5.957997898741896E-4 1.2018088859491465 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4357 0.4035810056642135 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3966 0.367363385004423 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2504 0.2319409773199887 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1999 0.18516374347550216 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 4.631409291533321E-5 0.0 24-25 0.0 0.0 0.0 9.262818583066641E-5 0.0 26-27 0.0 0.0 0.0 3.7051274332266566E-4 0.0 28-29 0.0 0.0 0.0 0.0011578523228833301 0.0 30-31 0.0 0.0 0.0 0.004214582455295322 0.0 32-33 0.0 0.0 0.0 0.008845991746828642 0.0 34-35 0.0 0.0 0.0 0.011949035972155967 0.0 36-37 0.0 0.0 0.0 0.01630256070619729 0.0 38-39 0.0 0.0 0.0 0.0249632960813646 0.0 40-41 0.0 0.0 0.0 0.03408717238568524 0.0 42-43 0.0 0.0 0.0 0.042562651389191214 0.0 44-45 0.0 0.0 0.0 0.048120342539031205 0.0 46-47 0.0 0.0 0.0 0.05516008466216185 0.0 48-49 0.0 0.0 0.0 0.0633113650152605 0.0 50-51 0.0 0.0 0.0 0.07312995271331113 0.0 52-53 0.0 0.0 0.0 0.09100719257862976 0.0 54-55 0.0 0.0 0.0 0.10522561910363705 0.0 56-57 0.0 0.0 0.0 0.14533362356831558 0.0 58-59 0.0 0.0 0.0 0.21114594960100408 0.0 60-61 0.0 9.262818583066641E-5 0.0 0.2678343993293719 0.0 62-63 0.0 9.262818583066641E-5 0.0 0.30340362268834786 0.0 64-65 0.0 9.262818583066641E-5 0.0 0.36296354617746635 0.0 66-67 0.0 9.262818583066641E-5 0.0 0.46411352510455406 0.0 68-69 0.0 9.262818583066641E-5 0.0 0.6081040399783251 0.0 70-71 0.0 9.262818583066641E-5 0.0 0.749593593834668 0.0 72-73 0.0 9.262818583066641E-5 0.0 0.9085898748130068 0.0 74-75 0.0 9.262818583066641E-5 0.0 1.0989407966950262 0.0 76-77 0.0 9.262818583066641E-5 0.0 1.2405229787372 0.0 78-79 0.0 9.262818583066641E-5 0.0 1.395119420888582 0.0 80-81 0.0 9.262818583066641E-5 0.0 1.5649995137020243 0.0 82-83 0.0 9.262818583066641E-5 0.0 1.738399477577032 0.0 84-85 0.0 9.262818583066641E-5 0.0 1.945099274258164 0.0 86-87 0.0 9.262818583066641E-5 0.0 2.245075654070777 0.0 88-89 0.0 9.262818583066641E-5 0.0 2.670053770661875 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 5860 0.0 26.357723 1 GGTATCA 4400 0.0 25.27466 1 GTACATG 3310 0.0 23.690712 1 GCACCGC 205 2.671004E-8 23.16897 6 GAGTACT 3995 0.0 22.118586 12-13 TCAACGC 6920 0.0 21.965681 4 ATCAACG 6930 0.0 21.933985 3 CAACGCA 6950 0.0 21.870867 5 TACATGG 3505 0.0 21.683668 2 AACGCAG 7070 0.0 21.564838 6 TATCAAC 7095 0.0 21.557793 2 ACATGGG 3365 0.0 21.03304 3 GTACTTT 4165 0.0 20.811743 14-15 AGTACTT 4200 0.0 20.36031 12-13 TGGACCG 190 8.249308E-6 20.00033 5 AGAGTAC 5920 0.0 19.901745 10-11 CTATACC 265 1.8540959E-8 19.717308 4 CTACGCA 145 6.1450206E-4 19.655499 4 ACGCAGA 7920 0.0 19.254889 7 ATTCGCG 75 7.7280117E-4 18.998556 80-81 >>END_MODULE