FastQCFastQC Report
Thu 26 May 2016
SRR937676_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937676_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848862
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25790.3038185240946114No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21040.24786125424391714No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13490.1589186463759716No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11570.13630012887842782No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10200.12016087420570129No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36750.028.7231851
GTACATG25100.027.8471371
GCGCAAT350.00839440327.1417172-73
TACATGG25800.026.7103752
ACATGGG26600.024.8335723
GGTATCA25500.024.240421
ATCAACG42750.024.1229083
CAACGCA42900.023.9277845
TCAACGC43250.023.844034
AACGCAG44500.023.27286
TATCAAC44700.023.0719282
TATCGTG1302.937944E-421.9260336
ACGCAGA47900.021.3233347
AGTACTT25400.021.31799512-13
CGCAGAG47950.021.2020248
AGAGTAC36850.021.13886310-11
GAGTACT23850.020.8114912-13
GTGTAGG3950.020.4639191
GGTATAC1656.2964624E-520.1613753
GTCTTAC2601.4713805E-820.1166781
Produced by FastQC (version 0.11.5)