##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937676_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 848862 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.842417259813725 33.0 31.0 34.0 30.0 34.0 2 31.967611932210417 33.0 31.0 34.0 30.0 34.0 3 32.01973347846882 34.0 31.0 34.0 30.0 34.0 4 35.598507177845164 37.0 35.0 37.0 33.0 37.0 5 35.59251209266053 37.0 35.0 37.0 33.0 37.0 6 35.64771658997576 37.0 35.0 37.0 33.0 37.0 7 35.5659624297 37.0 35.0 37.0 33.0 37.0 8 35.56623455873864 37.0 35.0 37.0 33.0 37.0 9 37.13515035423897 39.0 37.0 39.0 34.0 39.0 10-11 37.072474088838945 39.0 37.0 39.0 34.0 39.0 12-13 36.9762658712488 39.0 37.0 39.0 33.0 39.0 14-15 38.1555894833318 40.0 38.0 41.0 33.0 41.0 16-17 38.068084682787074 40.0 38.0 41.0 33.0 41.0 18-19 37.96683265359976 40.0 38.0 41.0 33.0 41.0 20-21 37.962687692463554 40.0 38.0 41.0 33.0 41.0 22-23 37.96831758283443 40.0 38.0 41.0 33.0 41.0 24-25 37.897766067982786 40.0 38.0 41.0 33.0 41.0 26-27 37.78831718229819 40.0 38.0 41.0 33.0 41.0 28-29 37.640264848703325 40.0 37.5 41.0 32.5 41.0 30-31 37.424636159941194 40.0 37.0 41.0 32.0 41.0 32-33 37.32444849692883 40.0 37.0 41.0 32.0 41.0 34-35 37.326212034464966 40.0 37.0 41.0 32.0 41.0 36-37 37.18167381741673 40.0 37.0 41.0 31.0 41.0 38-39 37.02858179539194 40.0 37.0 41.0 31.0 41.0 40-41 36.817568108832766 39.5 36.0 41.0 30.5 41.0 42-43 36.57124597402169 39.0 36.0 41.0 30.0 41.0 44-45 36.334910150295336 39.0 36.0 41.0 30.0 41.0 46-47 35.99958532717921 39.0 35.5 40.5 29.5 41.0 48-49 35.67183358425751 39.0 35.0 40.0 27.5 41.0 50-51 34.8407738831518 38.0 34.0 39.5 26.5 40.5 52-53 34.97722362409909 38.0 34.5 39.5 27.0 40.5 54-55 35.23760575924002 39.0 35.0 40.0 27.0 41.0 56-57 34.98385662216002 38.0 34.5 40.0 26.0 41.0 58-59 34.69618206492928 38.0 34.0 40.0 25.5 41.0 60-61 34.323521962344884 38.0 34.0 40.0 24.0 41.0 62-63 33.952150644038724 37.5 33.5 40.0 23.0 41.0 64-65 33.505196957809396 37.0 33.0 40.0 22.0 41.0 66-67 32.946503082951054 36.0 32.0 39.0 20.0 41.0 68-69 32.48921438349225 36.0 31.5 39.0 18.5 40.0 70-71 32.01960271516454 35.0 31.0 38.5 17.0 40.0 72-73 31.526491938619 35.0 31.0 38.0 12.0 40.0 74-75 30.95185789916382 35.0 30.0 37.0 7.5 39.0 76-77 30.376818611270146 34.0 30.0 36.5 6.5 39.0 78-79 29.81452638944846 34.0 29.0 36.0 2.0 38.5 80-81 29.227803812633855 34.0 29.0 35.5 2.0 37.5 82-83 28.6457651538177 34.0 28.0 35.0 2.0 37.0 84-85 28.06557956416944 33.5 26.5 35.0 2.0 36.5 86-87 27.445520591097257 33.0 26.0 35.0 2.0 36.0 88-89 26.856293484688912 32.5 25.0 35.0 2.0 35.5 90-91 26.255137466396185 32.0 24.5 34.5 2.0 35.0 92-93 25.539368589947482 32.0 21.5 34.0 2.0 35.0 94-95 24.72005932648652 31.0 18.5 34.0 2.0 35.0 96-97 23.79639505596905 31.0 4.5 34.0 2.0 35.0 98-99 22.552415469181092 30.5 2.0 34.0 2.0 35.0 100-101 19.263699517707238 25.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.11019275328259326 1101 2 -0.15076287426838064 1101 3 -0.2298371042357843 1101 4 -0.047202727360307506 1101 5 -0.07541657465478124 1101 6 -0.1646964102833337 1101 7 -0.17145848864583257 1101 8 -0.06690540490091479 1101 9 0.05373887360318008 1101 10-11 -0.001078851938999037 1101 12-13 0.0663215194395832 1101 14-15 0.0909115341046487 1101 16-17 0.17305902262112483 1101 18-19 0.17207421409501933 1101 20-21 0.14901060049459147 1101 22-23 0.054840701060300034 1101 24-25 0.004529377172467264 1101 26-27 0.29726600514821655 1101 28-29 -0.028790441475671003 1101 30-31 0.08323212558286741 1101 32-33 0.0321367611362291 1101 34-35 0.05526996135731821 1101 36-37 0.22018482408227413 1101 38-39 0.20566705411125952 1101 40-41 0.2870089985513786 1101 42-43 0.19274863875596537 1101 44-45 0.42376713701290925 1101 46-47 0.4308148335429678 1101 48-49 0.5045774308993458 1101 50-51 0.47036134080111225 1101 52-53 0.6019148931567102 1101 54-55 0.45242260778020693 1101 56-57 0.5541693731658697 1101 58-59 0.6296086098628706 1101 60-61 0.7220722780779596 1101 62-63 0.6761946752368289 1101 64-65 0.49024318075463214 1101 66-67 0.5010974942067463 1101 68-69 0.6928061710166773 1101 70-71 0.6076230063735295 1101 72-73 0.7965082819283502 1101 74-75 0.7711605471476553 1101 76-77 0.6725238852430415 1101 78-79 1.0334849115876814 1101 80-81 1.3104456775347408 1101 82-83 1.1730397921485611 1101 84-85 1.3266885483269704 1101 86-87 1.5974685229848546 1101 88-89 1.5479141364583988 1101 90-91 1.709622798705837 1101 92-93 1.6487367411675926 1101 94-95 1.9878691270821456 1101 96-97 2.2588576135082974 1101 98-99 2.505955348693135 1101 100-101 3.1811819682593807 1102 1 0.09172187951133992 1102 2 0.05230569344362124 1102 3 0.10979741310470814 1102 4 0.12420905956036421 1102 5 0.16343596445162234 1102 6 0.04024392978278257 1102 7 0.21818487053364066 1102 8 0.10575023394442695 1102 9 0.11887325456955011 1102 10-11 0.27838395389122184 1102 12-13 0.11843966439180065 1102 14-15 0.3849341512290465 1102 16-17 0.4359868402454623 1102 18-19 0.22504956784351293 1102 20-21 0.2373503129400092 1102 22-23 0.2623901329346765 1102 24-25 0.23512004198884284 1102 26-27 0.2672494604190021 1102 28-29 0.21117482058745196 1102 30-31 0.4683024556682014 1102 32-33 0.38474180667920166 1102 34-35 0.395323342086904 1102 36-37 0.609731522479656 1102 38-39 0.513031107090363 1102 40-41 0.5208921244261973 1102 42-43 0.4154730641113602 1102 44-45 0.4820430983006432 1102 46-47 0.6695534777743362 1102 48-49 0.8596881636929723 1102 50-51 0.7271373365570284 1102 52-53 0.7504083337110785 1102 54-55 0.9640743532191678 1102 56-57 0.7614168337597391 1102 58-59 0.631410696536868 1102 60-61 0.7663444148956771 1102 62-63 0.633765837054483 1102 64-65 0.8455037752259358 1102 66-67 1.0041973830093838 1102 68-69 0.9710985571442805 1102 70-71 1.085968743258391 1102 72-73 1.0139028767173635 1102 74-75 0.9781187747036206 1102 76-77 0.9377618206286797 1102 78-79 1.198517836198267 1102 80-81 1.2138553304251225 1102 82-83 1.369928813581776 1102 84-85 1.5549563571394351 1102 86-87 1.8068372756261653 1102 88-89 1.755498785827033 1102 90-91 1.7564216034090947 1102 92-93 1.6899202288084965 1102 94-95 2.101409579244798 1102 96-97 2.0866890251153833 1102 98-99 2.3935448535801243 1102 100-101 2.794139458495895 1103 1 -3.1171386215333996E-5 1103 2 -0.11087592888250342 1103 3 0.015560555911999785 1103 4 -0.022387146536807734 1103 5 0.1485438097185039 1103 6 0.03833522702407066 1103 7 0.02431266103214824 1103 8 -0.10121403841606025 1103 9 -0.08571582238037223 1103 10-11 0.02330416940370128 1103 12-13 0.1114387136014301 1103 14-15 0.13157522608508998 1103 16-17 0.21219945406431862 1103 18-19 0.052922681842211716 1103 20-21 0.20380379365177959 1103 22-23 0.0876904153229745 1103 24-25 -0.07701428652881503 1103 26-27 0.022289183514665467 1103 28-29 -0.06079751746660378 1103 30-31 -0.22375719841502928 1103 32-33 -0.17831660461063592 1103 34-35 -0.07342966251748351 1103 36-37 0.04082147721918972 1103 38-39 0.08311628651198077 1103 40-41 -0.047727698306594846 1103 42-43 0.10984915689710562 1103 44-45 0.2967698439980424 1103 46-47 0.1814063082452151 1103 48-49 0.16121512589995035 1103 50-51 0.17576972966433146 1103 52-53 0.2129206637172345 1103 54-55 0.27321119937273153 1103 56-57 0.19499771681964972 1103 58-59 0.15186604321925756 1103 60-61 0.44703731672924363 1103 62-63 0.31225091107012304 1103 64-65 0.3270132172508653 1103 66-67 0.6059497573145407 1103 68-69 0.6084842041268601 1103 70-71 0.4037045446108607 1103 72-73 0.3825139749692603 1103 74-75 0.3757058325593938 1103 76-77 0.48482950479535347 1103 78-79 0.706682347858802 1103 80-81 0.7317725653700542 1103 82-83 0.9229095591865963 1103 84-85 1.189657890867565 1103 86-87 1.2057045175150698 1103 88-89 1.3605949509725512 1103 90-91 1.4069358136299144 1103 92-93 1.725137753263752 1103 94-95 1.736404006260063 1103 96-97 1.5021568175368394 1103 98-99 1.8314532572137807 1103 100-101 2.701986683932919 1104 1 0.33021586597974206 1104 2 0.3109133970406681 1104 3 0.23486040980949952 1104 4 0.17813423885867508 1104 5 0.13726717943422528 1104 6 0.19018347140676184 1104 7 0.3860930107232363 1104 8 0.3249889199208553 1104 9 0.4202753350761057 1104 10-11 0.27864932462566827 1104 12-13 0.1905641994161158 1104 14-15 0.3416505691394889 1104 16-17 0.3833303638149914 1104 18-19 0.5414120965727847 1104 20-21 0.4684517630180025 1104 22-23 0.3256928217171122 1104 24-25 0.37203795705175224 1104 26-27 0.27672573396777267 1104 28-29 0.3763807944288189 1104 30-31 0.4445266224040978 1104 32-33 0.5247749434074365 1104 34-35 0.5886161544628763 1104 36-37 0.4391618292020709 1104 38-39 0.6294379411944178 1104 40-41 0.7320547713031331 1104 42-43 0.8009736686951214 1104 44-45 0.8766396886328351 1104 46-47 1.003096800123636 1104 48-49 1.10122906770723 1104 50-51 0.8891514773558171 1104 52-53 0.9180137205062309 1104 54-55 1.0434774305966599 1104 56-57 0.8427121198524645 1104 58-59 0.9332475235336162 1104 60-61 1.0370350249253022 1104 62-63 1.0391734003511672 1104 64-65 1.0496910946584563 1104 66-67 1.3578705754885476 1104 68-69 1.1399038811734883 1104 70-71 1.4358413653058477 1104 72-73 1.2398501348607844 1104 74-75 1.30533981237242 1104 76-77 1.3126443652340392 1104 78-79 1.3197876467004832 1104 80-81 1.8189656784992216 1104 82-83 1.83137252268007 1104 84-85 2.2127702561047187 1104 86-87 2.189209736143617 1104 88-89 2.0424873125810166 1104 90-91 2.2817536629629025 1104 92-93 2.5484707046297608 1104 94-95 2.779311350536762 1104 96-97 2.7571830300443594 1104 98-99 3.0160418306613224 1104 100-101 3.6788217981888955 1105 1 0.2804490749349604 1105 2 0.28158348410534373 1105 3 0.2649482518492974 1105 4 0.21457702492832453 1105 5 0.09968944311581396 1105 6 0.2547784651878544 1105 7 0.16868396221577342 1105 8 0.2203480617119027 1105 9 0.39171889850894104 1105 10-11 0.3095300771132301 1105 12-13 0.34408176782407907 1105 14-15 0.4995174845623751 1105 16-17 0.5980672263771822 1105 18-19 0.4797361015478998 1105 20-21 0.5798634545602965 1105 22-23 0.522752445473337 1105 24-25 0.46889352297214515 1105 26-27 0.4110910169279691 1105 28-29 0.49493839704076237 1105 30-31 0.6185082766329515 1105 32-33 0.6491686001238506 1105 34-35 0.9006186420250586 1105 36-37 0.860587901963271 1105 38-39 0.8746454971645719 1105 40-41 0.761612062969796 1105 42-43 0.647871989590648 1105 44-45 0.6727995145034811 1105 46-47 0.8674955097007526 1105 48-49 0.6801178270355805 1105 50-51 0.6269216110622935 1105 52-53 0.7601642957549828 1105 54-55 0.6493095978852139 1105 56-57 0.6500271074146582 1105 58-59 0.7639514258967992 1105 60-61 0.7504814646019256 1105 62-63 1.1314600141571418 1105 64-65 1.040424925504226 1105 66-67 1.1627640767323228 1105 68-69 1.193332052815279 1105 70-71 1.3063831874451495 1105 72-73 1.4297288662040621 1105 74-75 1.327170113989336 1105 76-77 1.220379128915635 1105 78-79 1.7258724570238684 1105 80-81 1.719556474519127 1105 82-83 1.8629062602422586 1105 84-85 2.319072728119643 1105 86-87 2.36666426039735 1105 88-89 1.9800417280039042 1105 90-91 1.9764773103509654 1105 92-93 1.751080701520312 1105 94-95 1.807786163969599 1105 96-97 1.9650674361388845 1105 98-99 2.6976960595170425 1105 100-101 3.059974627790883 1106 1 -0.06697584046266769 1106 2 0.04383760847082385 1106 3 0.057256045842528636 1106 4 0.02697127196724125 1106 5 -0.12717709664730137 1106 6 -0.058204032697084074 1106 7 -0.03295388676900046 1106 8 -0.04182674793276675 1106 9 0.07457237778136516 1106 10-11 -0.23532900706612025 1106 12-13 -0.36279085273429246 1106 14-15 -0.6125277777049618 1106 16-17 -0.20413838381740135 1106 18-19 0.12749942642099654 1106 20-21 0.010225129687199797 1106 22-23 0.011446792977558573 1106 24-25 -0.1852744656065326 1106 26-27 0.08732502179260848 1106 28-29 -0.047729617976166594 1106 30-31 0.12000203720825198 1106 32-33 0.16579825325413822 1106 34-35 0.4882878467627947 1106 36-37 0.13947498423907234 1106 38-39 0.20350415833208757 1106 40-41 0.11598032121852242 1106 42-43 0.22957937114733085 1106 44-45 0.18541055511261817 1106 46-47 0.4271567036516828 1106 48-49 0.6447071089729803 1106 50-51 0.6303288392771762 1106 52-53 0.643773247743141 1106 54-55 0.4838491759308994 1106 56-57 0.39855978507962675 1106 58-59 0.3035129476819094 1106 60-61 0.7389114297034496 1106 62-63 0.30334492849723915 1106 64-65 0.5631618121370536 1106 66-67 0.8277849629421397 1106 68-69 0.46084738699463657 1106 70-71 0.7006897124028555 1106 72-73 0.49045750410592603 1106 74-75 0.9538258087862879 1106 76-77 1.1480308844147835 1106 78-79 1.1409230862539843 1106 80-81 0.9885391310335088 1106 82-83 1.328675348651732 1106 84-85 0.9364816483819141 1106 86-87 1.1865504437729832 1106 88-89 1.552945175550434 1106 90-91 1.785728711577697 1106 92-93 1.6816373586692102 1106 94-95 1.8212564473875332 1106 96-97 2.1402373018326273 1106 98-99 2.344586922461204 1106 100-101 2.4939517322748053 1107 1 0.14678105447768175 1107 2 0.1893658998694825 1107 3 0.0445107142440051 1107 4 0.1918634268537147 1107 5 0.21842816152736333 1107 6 0.1350009005067463 1107 7 0.2368482597488608 1107 8 0.18656910563488793 1107 9 0.06636258834047482 1107 10-11 0.2764985262708777 1107 12-13 0.2942030448998878 1107 14-15 0.5609269310124461 1107 16-17 0.42236353729775544 1107 18-19 0.32459545774525367 1107 20-21 0.413309603778103 1107 22-23 0.21338073593663154 1107 24-25 0.4226531123223225 1107 26-27 0.3528756702071547 1107 28-29 0.3454973057351012 1107 30-31 0.3606957766329515 1107 32-33 0.38572470722373 1107 34-35 0.40911743865563466 1107 36-37 0.2603815429079148 1107 38-39 0.318138841027384 1107 40-41 0.46027378454019185 1107 42-43 0.24584318393480942 1107 44-45 0.45011976119422314 1107 46-47 0.4901381277813712 1107 48-49 0.46348547520045 1107 50-51 0.2003059935833491 1107 52-53 0.2675278825480021 1107 54-55 0.4611256515855757 1107 56-57 0.7252470983893886 1107 58-59 0.5704792350123213 1107 60-61 0.7068663735971086 1107 62-63 0.523972329891194 1107 64-65 0.5776541673814819 1107 66-67 0.8122404230018816 1107 68-69 0.8121365804927692 1107 70-71 0.9773791636786058 1107 72-73 1.0403072356384762 1107 74-75 1.107566943862981 1107 76-77 1.2680532414306747 1107 78-79 1.216261953865022 1107 80-81 1.1458124913662964 1107 82-83 1.3819580427332347 1107 84-85 1.455182742409658 1107 86-87 1.473037887804086 1107 88-89 1.8109213158498711 1107 90-91 1.6904349855916365 1107 92-93 1.9078830292880617 1107 94-95 2.23598825482399 1107 96-97 2.5290645969391257 1107 98-99 2.9221996696253427 1107 100-101 3.209695408476801 1108 1 0.34130979870654343 1108 2 0.2802658380230021 1108 3 0.2549829049037484 1108 4 0.17589840607042362 1108 5 0.14295388534689124 1108 6 0.20792940310286667 1108 7 0.05038113187048765 1108 8 0.15254635188454557 1108 9 0.43777944797773216 1108 10-11 0.5021087208715329 1108 12-13 0.3391172508918032 1108 14-15 0.4028624380816339 1108 16-17 0.4659005804608469 1108 18-19 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2316 62-63 -1.5752291631027475 2316 64-65 -1.1654366564166807 2316 66-67 -1.4941700687478985 2316 68-69 -1.5092191477496968 2316 70-71 -1.3844934015378954 2316 72-73 -1.6671108230614742 2316 74-75 -1.9318892963213976 2316 76-77 -1.8897462241917111 2316 78-79 -2.025793768258616 2316 80-81 -1.9695325085317243 2316 82-83 -2.1908764021871185 2316 84-85 -2.173236152530073 2316 86-87 -2.2183794366365532 2316 88-89 -2.705472043182535 2316 90-91 -2.773622001089713 2316 92-93 -2.8546197751746085 2316 94-95 -3.0055524659739064 2316 96-97 -2.814737030669022 2316 98-99 -2.8831655224038606 2316 100-101 -3.0288980770113767 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3539.0 3 702.0 4 676.0 5 740.0 6 830.0 7 944.0 8 1165.0 9 1491.0 10 1788.0 11 2262.0 12 2874.0 13 3726.0 14 4152.0 15 4984.0 16 6146.0 17 8005.0 18 7264.0 19 5632.0 20 5692.0 21 6239.0 22 6777.0 23 7702.0 24 8691.0 25 9854.0 26 11419.0 27 13493.0 28 15957.0 29 18848.0 30 23345.0 31 28327.0 32 36224.0 33 47381.0 34 63539.0 35 88442.0 36 125877.0 37 160743.0 38 104870.0 39 8522.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.210924066863235 18.837752081351443 14.717946596864826 23.233377254920498 2 14.731300780839376 19.439250791056075 41.792446256022245 24.037002172082307 3 19.251313526487877 23.526062633478862 30.196461983330497 27.026161856702764 4 12.476952823714644 16.18162769464455 37.9798566793404 33.361562802300405 5 13.483317269531693 36.97402861246551 35.20842782628416 14.334226291718638 6 32.075168780331175 35.808581442486016 17.79645310450746 14.319796672675352 7 28.32970366566925 32.37014347515596 21.124665804989466 18.175487054185332 8 25.42496128130548 35.676443923998036 20.175981188380614 18.722613606315868 9 25.587550273338223 17.04577515083477 21.087474132914032 36.27920044291298 10-11 24.56347562062546 26.491667345461927 28.06334399337024 20.881513040542373 12-13 25.097204489203467 23.80679165272128 28.67525052992458 22.42075332815067 14-15 22.028929219857822 25.39582010194758 26.75297661910844 25.822274059086155 16-17 21.523555008903102 28.810053158934412 27.51516360522276 22.151228226939722 18-19 21.476981402880924 27.537805643817247 29.663561638661612 21.321651314640214 20-21 21.999799347288533 26.540629223296957 29.81067826518026 21.648893164234245 22-23 21.774921769964152 26.41790833363827 29.803725532088183 22.0034443643094 24-25 21.827781456601876 26.55227687735115 29.815000395424406 21.804941270622564 26-27 21.62135522353527 26.73398753684469 29.783671354220154 21.860985885399884 28-29 21.258416673749423 26.976796894920245 30.001192263742904 21.763594167587424 30-31 21.565279289446284 26.676161650025854 29.83813276607399 21.920426294453875 32-33 21.357644036905178 26.731405758643156 29.833947947456394 22.077002256995268 34-35 21.877958546702835 26.71805241747942 29.625366830279464 21.77862220553828 36-37 21.74453192318134 26.568833437599594 29.749111769496807 21.937522869722258 38-39 21.50232220523927 26.87762243952529 29.822131471634865 21.79792388360057 40-41 21.67741174040689 26.82424796777953 29.75383208179042 21.744508210023163 42-43 21.61785249103085 26.758611653906144 29.48532800918781 22.138207845875193 44-45 21.655231105356282 26.84956876150097 29.40554360129885 22.089656531843897 46-47 21.813920629758794 27.09844348584099 29.105097313857776 21.982538570542438 48-49 21.796034101345498 27.16952221757905 28.900559777694358 22.133883903381097 50-51 21.60439725177666 27.273065298338988 29.051112208889528 22.071425240994824 52-53 21.800957378365137 27.40185691266136 28.69113037935085 22.106055329622656 54-55 21.668683196307683 27.217661258432535 28.834170439052603 22.279485106207172 56-57 21.753048843454643 27.43684717692468 28.729555621514418 22.08054835810626 58-59 21.97738852977063 27.231587617382107 28.703185807709282 22.08783804513798 60-61 21.803742360287007 27.12405707781015 29.169614758417772 21.90258580348507 62-63 21.717893206398433 27.2666335049834 29.089877012639793 21.925596275978375 64-65 21.641377527807432 27.28394471618983 28.931648216895894 22.143029539106845 66-67 21.65315834973313 27.247814426115358 28.74577711424584 22.353250109905673 68-69 21.436373324647136 27.224259032728426 28.996372687344767 22.342994955279664 70-71 21.655161262529756 27.24697651198006 28.760992490112514 22.33686973537767 72-73 21.68346345260556 27.068979050098825 28.880104764259762 22.367452733035854 74-75 21.422509442870634 27.334454674220964 28.842127006610006 22.400908876298395 76-77 21.589500043378006 27.219316256365794 28.745395881863097 22.4457878183931 78-79 21.629791377733188 27.156127356959487 28.709138658561773 22.50494260674555 80-81 21.601282272209016 27.288768551282626 28.71781041161599 22.39213876489237 82-83 21.591942448080946 27.378055940927965 28.79802951595324 22.231972095037854 84-85 21.583781937011974 27.269084907502965 28.61645963924062 22.53067351624444 86-87 21.44602571297232 27.216544486675858 28.871477035413612 22.46595276493821 88-89 21.64073233026622 27.05153505148312 28.595143449298217 22.712589168952448 90-91 21.537144219466253 26.929382078433413 28.770423891645386 22.763049810454955 92-93 21.789371006869352 26.77671631961922 28.810258764602754 22.623653908908675 94-95 21.474912014427037 26.68405920939268 28.941588437598103 22.899440338582174 96-97 21.330911391389122 26.702086977458944 29.07309968439217 22.89390194675977 98-99 21.409940006821966 26.992681139150417 28.628536829768326 22.968842024259292 100-101 21.57391481364563 26.274830285203464 29.24381400261395 22.907440898536958 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1001.0 1 587.5 2 144.5 3 114.5 4 108.0 5 111.5 6 163.5 7 310.0 8 512.5 9 656.0 10 683.5 11 688.0 12 752.5 13 820.0 14 876.0 15 946.5 16 998.5 17 1039.0 18 1124.0 19 1271.5 20 1435.5 21 1672.5 22 2108.0 23 2627.5 24 3161.5 25 3927.0 26 5109.5 27 6513.5 28 7718.0 29 8817.5 30 10159.5 31 11808.5 32 13522.0 33 15390.0 34 17651.0 35 19676.0 36 21607.0 37 23543.0 38 25089.0 39 26342.5 40 27310.0 41 28948.0 42 30378.5 43 31659.0 44 33384.0 45 34409.0 46 34448.5 47 34911.0 48 36738.5 49 36087.5 50 33291.5 51 30991.5 52 28567.5 53 26807.5 54 25173.5 55 22862.0 56 20549.0 57 18224.0 58 15933.5 59 13554.0 60 10959.5 61 8522.0 62 6595.0 63 5006.0 64 3682.5 65 2754.0 66 1987.0 67 1435.0 68 1004.0 69 694.0 70 506.5 71 340.0 72 245.5 73 173.5 74 129.5 75 104.0 76 61.5 77 43.5 78 27.5 79 18.0 80 12.5 81 7.5 82 7.5 83 6.0 84 4.0 85 2.5 86 2.0 87 3.5 88 3.0 89 3.0 90 2.0 91 1.0 92 1.0 93 0.5 94 1.5 95 1.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.31300729682798856 2 0.26023075600038637 3 0.2693017239551305 4 0.26258685157304723 5 0.26364709458074453 6 0.20674738650098604 7 0.19755861376760886 8 0.20333104792062784 9 0.20533372915738954 10-11 0.20886787251638075 12-13 0.21293213737922065 14-15 0.20733641039415124 16-17 0.1985599543859897 18-19 0.19249300828638813 20-21 0.19166837483595686 22-23 0.19802983288214104 24-25 0.19685178509581064 26-27 0.20321324314199482 28-29 0.20427348614969215 30-31 0.20592275305055474 32-33 0.20238860969156353 34-35 0.2049803148214904 36-37 0.19696958987444366 38-39 0.18931227926329602 40-41 0.18548362395772222 42-43 0.19467239669109937 44-45 0.19449568952314983 46-47 0.1979709304928245 48-49 0.2049803148214904 50-51 0.19985580695095315 52-53 0.2069829960582521 54-55 0.19991470934026967 56-57 0.1805358232551345 58-59 0.1668115665443853 60-61 0.18406996661412572 62-63 0.17653046078161116 64-65 0.1839521618354927 66-67 0.181949480598731 68-69 0.18147826148419885 70-71 0.1860137454615709 72-73 0.1916094724466403 74-75 0.19579154208811325 76-77 0.19537922536289762 78-79 0.19284642262228724 80-81 0.1903725222709934 82-83 0.18348094272096052 84-85 0.17794411812520763 86-87 0.1821850901559971 88-89 0.1877808171410665 90-91 0.18495350245387354 92-93 0.18230289493463012 94-95 0.1842466737820753 96-97 0.18943008404192907 98-99 0.1883698410342317 100-101 0.17488119388074858 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 848862.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.31977712975538 #Duplication Level Percentage of deduplicated Percentage of total 1 83.84551388026415 58.121523355097224 2 10.048227862701964 13.930818319829969 3 2.7590904972969588 5.737786150603534 4 1.1191557999072994 3.1031852249218836 5 0.6094709990350575 2.112419691007977 6 0.3595347949636742 1.4953723114364514 7 0.23690259421723414 1.1495424522819682 8 0.16938765797512 0.9393531759493241 9 0.12526431789901032 0.78149651391632 >10 0.678138411220893 8.472771501319896 >50 0.03668774204246779 1.6900528988709311 >100 0.011089915758905958 1.1975949723692099 >500 6.824563187674114E-4 0.297210750383266 >1k 8.530703984592642E-4 0.9708726820120296 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2579 0.3038185240946114 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2104 0.24786125424391714 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1349 0.1589186463759716 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1157 0.13630012887842782 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1020 0.12016087420570129 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 1.1780477863304048E-4 0.0 12-13 0.0 0.0 0.0 2.3560955726608095E-4 0.0 14-15 0.0 0.0 0.0 3.534143358991214E-4 0.0 16-17 0.0 0.0 0.0 3.534143358991214E-4 0.0 18-19 0.0 0.0 0.0 4.712191145321619E-4 0.0 20-21 0.0 0.0 0.0 4.712191145321619E-4 0.0 22-23 0.0 0.0 0.0 7.068286717982427E-4 0.0 24-25 0.0 0.0 0.0 9.424382290643238E-4 0.0 26-27 0.0 0.0 0.0 0.0013547549542799655 0.0 28-29 0.0 0.0 0.0 0.0024149979619773297 0.0 30-31 0.0 0.0 0.0 0.005713531763702463 0.0 32-33 0.0 0.0 0.0 0.008953163176111077 0.0 34-35 0.0 0.0 0.0 0.011309258748771884 0.0 36-37 0.0 0.0 0.0 0.01590364511546046 0.0 38-39 0.0 0.0 0.0 0.022971931833442892 0.0 40-41 0.0 0.0 0.0 0.031100461559122685 0.0 42-43 0.0 0.0 0.0 0.03940569845275203 0.0 44-45 0.0 0.0 0.0 0.04370557287285801 0.0 46-47 0.0 0.0 0.0 0.05030264047630828 0.0 48-49 0.0 0.0 0.0 0.059609217988318475 0.0 50-51 0.0 0.0 0.0 0.0686801859430626 0.0 52-53 0.0 0.0 0.0 0.0864687075166517 0.0 54-55 0.0 0.0 0.0 0.0992505259983366 0.0 56-57 0.0 0.0 0.0 0.14077671046648338 0.0 58-59 0.0 0.0 0.0 0.2046269004855913 0.0 60-61 0.0 0.0 0.0 0.26235124201578114 0.0 62-63 0.0 0.0 0.0 0.2948064585291838 0.0 64-65 0.0 0.0 0.0 0.35094043554782756 0.0 66-67 0.0 0.0 0.0 0.4586729056077431 0.0 68-69 0.0 0.0 0.0 0.6181805758768799 0.0 70-71 0.0 0.0 0.0 0.7763334911917368 0.0 72-73 0.0 0.0 0.0 0.954454316484894 0.0 74-75 0.0 0.0 0.0 1.168917915986344 0.0 76-77 0.0 0.0 0.0 1.3220052258199804 0.0 78-79 0.0 0.0 0.0 1.4910550831583933 0.0 80-81 0.0 0.0 0.0 1.6678800558865872 0.0 82-83 0.0 0.0 0.0 1.846884417019492 0.0 84-85 0.0 0.0 0.0 2.0553988751999737 0.0 86-87 0.0 0.0 0.0 2.370114341318141 0.0 88-89 0.0 0.0 0.0 2.857060393797814 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 3675 0.0 28.723185 1 GTACATG 2510 0.0 27.847137 1 GCGCAAT 35 0.008394403 27.14171 72-73 TACATGG 2580 0.0 26.710375 2 ACATGGG 2660 0.0 24.833572 3 GGTATCA 2550 0.0 24.24042 1 ATCAACG 4275 0.0 24.122908 3 CAACGCA 4290 0.0 23.927784 5 TCAACGC 4325 0.0 23.84403 4 AACGCAG 4450 0.0 23.2728 6 TATCAAC 4470 0.0 23.071928 2 TATCGTG 130 2.937944E-4 21.926033 6 ACGCAGA 4790 0.0 21.323334 7 AGTACTT 2540 0.0 21.317995 12-13 CGCAGAG 4795 0.0 21.202024 8 AGAGTAC 3685 0.0 21.138863 10-11 GAGTACT 2385 0.0 20.81149 12-13 GTGTAGG 395 0.0 20.463919 1 GGTATAC 165 6.2964624E-5 20.161375 3 GTCTTAC 260 1.4713805E-8 20.116678 1 >>END_MODULE