FastQCFastQC Report
Thu 26 May 2016
SRR937676_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937676_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences848862
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35450.41761794025412846No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31470.37073163835817835No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20800.2450339395567242No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16650.1961449564240124No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49150.026.4005951
GTACATG27250.025.117161
GGTATCA36200.025.0783731
TACATGG28400.023.9172232
ACATGGG27700.023.4927523
ATCAACG56900.022.7064723
TCAACGC57650.022.4110744
CAACGCA57800.022.3529135
AACGCAG59500.021.7928076
GAGTACT35550.021.78172312-13
TATCAAC59250.021.6455422
ACTATAC2257.146264E-821.1110843
GTACTTT36350.021.03724914-15
CATGGGG13000.020.8268974
AGTACTT36500.020.69419312-13
GGACCGA1404.8522677E-420.3559196
ATATAGG3052.055458E-1020.2590051
AGAGTAC49850.020.20295710-11
CCTACAC2902.4356268E-919.6551483
GCACCGT1707.9544065E-519.5576486
Produced by FastQC (version 0.11.5)