##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937676_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 848862 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.464663278601236 33.0 31.0 34.0 31.0 34.0 2 32.71012720559997 34.0 31.0 34.0 31.0 34.0 3 32.80803593516968 34.0 31.0 34.0 31.0 34.0 4 36.33985736197403 37.0 37.0 37.0 35.0 37.0 5 36.220924013561685 37.0 35.0 37.0 35.0 37.0 6 36.30732321625894 37.0 37.0 37.0 35.0 37.0 7 36.27115243702745 37.0 37.0 37.0 35.0 37.0 8 36.264848703322805 37.0 37.0 37.0 35.0 37.0 9 37.993111954593324 39.0 38.0 39.0 35.0 39.0 10-11 37.94048502583459 39.0 38.0 39.0 35.0 39.0 12-13 37.87768388736921 39.0 38.0 39.0 35.0 39.0 14-15 39.20054555392985 40.0 38.5 41.0 36.0 41.0 16-17 39.17206801576699 40.0 38.0 41.0 36.0 41.0 18-19 39.13801242133586 40.0 38.5 41.0 36.0 41.0 20-21 39.05762244039667 40.0 38.5 41.0 36.0 41.0 22-23 38.90942167278074 40.0 38.0 41.0 35.0 41.0 24-25 38.86955241252406 40.0 38.0 41.0 35.0 41.0 26-27 38.81137452259614 40.0 38.0 41.0 35.0 41.0 28-29 38.70861164712285 40.0 38.0 41.0 35.0 41.0 30-31 38.79502380834576 40.0 38.0 41.0 35.0 41.0 32-33 38.7392273420179 40.0 38.0 41.0 35.0 41.0 34-35 38.627350499845676 40.0 38.0 41.0 35.0 41.0 36-37 38.49552577450751 40.0 38.0 41.0 35.0 41.0 38-39 38.37754252163485 40.0 38.0 41.0 34.5 41.0 40-41 38.21579773861947 40.0 38.0 41.0 34.0 41.0 42-43 38.03068814483391 40.0 38.0 41.0 34.0 41.0 44-45 37.758887192500076 40.0 38.0 41.0 33.0 41.0 46-47 37.429430814431555 40.0 37.0 41.0 33.0 41.0 48-49 37.129917465972085 40.0 37.0 41.0 32.0 41.0 50-51 36.88659110668165 40.0 37.0 41.0 31.0 41.0 52-53 36.68799934500543 40.0 36.5 41.0 31.0 41.0 54-55 36.4956647841463 39.0 36.0 41.0 31.0 41.0 56-57 36.233207517829754 39.0 36.0 40.5 30.5 41.0 58-59 35.99173304965943 39.0 35.0 40.0 30.0 41.0 60-61 35.65378412509925 38.5 35.0 40.0 29.0 41.0 62-63 35.30661520953936 38.0 35.0 40.0 28.5 41.0 64-65 34.918632239398164 37.5 34.0 40.0 28.0 41.0 66-67 34.50359481281998 37.0 34.0 39.5 27.5 41.0 68-69 34.058799310135214 36.5 34.0 39.0 26.0 40.5 70-71 33.645337522471266 36.0 33.0 39.0 26.0 40.0 72-73 33.19891631384135 35.5 33.0 38.0 26.0 40.0 74-75 32.7079354476935 35.0 32.5 37.5 25.0 39.5 76-77 31.497808242093534 34.0 30.5 36.0 23.5 39.0 78-79 32.021327966147624 35.0 32.0 36.0 25.0 39.0 80-81 31.79366669729591 35.0 32.0 36.0 24.0 38.0 82-83 31.445778583562465 35.0 32.0 36.0 23.0 37.0 84-85 31.06246951801353 34.0 31.0 35.0 21.0 37.0 86-87 30.64319111940457 34.0 31.0 35.0 19.5 36.0 88-89 30.23560425605104 34.0 31.0 35.0 18.0 36.0 90-91 29.752233578602883 34.0 30.0 35.0 12.5 35.5 92-93 29.312697470260183 34.0 29.5 35.0 4.5 35.0 94-95 28.87815333941206 34.0 29.0 35.0 2.0 35.0 96-97 28.388549611126425 34.0 29.0 35.0 2.0 35.0 98-99 27.639260562965475 33.5 28.0 35.0 2.0 35.0 100-101 25.22408412674852 31.0 22.0 34.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.1788554165816052 1101 2 0.1445373514305004 1101 3 0.08375751600634374 1101 4 0.0313291222876515 1101 5 -0.09319155383563782 1101 6 -0.015565201186511501 1101 7 0.008894685797635304 1101 8 0.06224443931800039 1101 9 0.08735511609646807 1101 10-11 0.10078508936607022 1101 12-13 0.19888018856399725 1101 14-15 0.1327837265879097 1101 16-17 -0.03137922470377674 1101 18-19 0.07610997450964874 1101 20-21 0.10323681524887718 1101 22-23 0.10951962293636797 1101 24-25 0.06845214779912823 1101 26-27 0.06169212416921255 1101 28-29 -0.07846408961193418 1101 30-31 -0.029783981597653053 1101 32-33 0.0013383673495184212 1101 34-35 -0.08918020154101924 1101 36-37 0.15934980672611943 1101 38-39 0.15197749920326942 1101 40-41 0.02890210590582143 1101 42-43 0.014249161338710792 1101 44-45 0.002499831330155189 1101 46-47 0.039609314886412506 1101 48-49 0.06367876743153289 1101 50-51 -0.09897757254240958 1101 52-53 -0.0479481913646751 1101 54-55 0.09282301879378707 1101 56-57 -0.0206652894920083 1101 58-59 -0.0012815352258996882 1101 60-61 -0.005565735211796152 1101 62-63 -0.0094300875499016 1101 64-65 -0.03746396254073403 1101 66-67 -0.09658404315828051 1101 68-69 -0.05525848105301634 1101 70-71 -0.069949819551546 1101 72-73 -0.07196347678607395 1101 74-75 -0.19767765670632542 1101 76-77 -0.18729597510884233 1101 78-79 0.09729295138352612 1101 80-81 0.1937764861761231 1101 82-83 0.02407120380739869 1101 84-85 0.21415090640277867 1101 86-87 0.27267524968675616 1101 88-89 0.5919277390138937 1101 90-91 0.5910231540075621 1101 92-93 0.6962402630155431 1101 94-95 0.5554355410431597 1101 96-97 0.5879607896474646 1101 98-99 0.772634624190772 1101 100-101 1.497938001186352 1102 1 0.07623913141281946 1102 2 0.10941576865140945 1102 3 0.06920744572121862 1102 4 0.08803078665144426 1102 5 -0.036732665389671126 1102 6 -0.020585567388508252 1102 7 0.004316411544770915 1102 8 0.00995170551130542 1102 9 0.027335514189026355 1102 10-11 -0.002912447807112528 1102 12-13 0.033471860450838165 1102 14-15 0.004373998766688203 1102 16-17 0.1107413480076147 1102 18-19 0.057664999221842095 1102 20-21 0.15475701210716863 1102 22-23 0.22085261258691702 1102 24-25 -0.02672200645649525 1102 26-27 -0.07923255235435533 1102 28-29 -0.07404841527934991 1102 30-31 -0.062175833917955003 1102 32-33 0.0478884016678478 1102 34-35 -0.018366812299284163 1102 36-37 0.049302630269984604 1102 38-39 0.04057857041454582 1102 40-41 -0.06748667724733082 1102 42-43 0.07202533393141408 1102 44-45 0.12160539689313765 1102 46-47 -0.014125837567874555 1102 48-49 -0.052605652049287244 1102 50-51 0.023163441301804255 1102 52-53 -0.11645820662059947 1102 54-55 0.045662298138950064 1102 56-57 0.2181228090929963 1102 58-59 0.19252183426405622 1102 60-61 0.1188299475729977 1102 62-63 -0.005109575382633125 1102 64-65 0.14475809649878357 1102 66-67 0.05286401628286086 1102 68-69 -0.006618625639568165 1102 70-71 0.05869069525328996 1102 72-73 0.008965575698475448 1102 74-75 0.17317005085958925 1102 76-77 -0.05202932622576384 1102 78-79 0.18880148900329985 1102 80-81 0.1256905794680172 1102 82-83 0.21644564220076035 1102 84-85 0.2027112152377235 1102 86-87 0.23578005395241775 1102 88-89 0.14786136227961677 1102 90-91 0.22993968405033272 1102 92-93 0.6542759960339666 1102 94-95 0.45291966319841137 1102 96-97 0.692839236840701 1102 98-99 0.8145854036213578 1102 100-101 1.6585005519519918 1103 1 0.07121632312705373 1103 2 0.026034658398891963 1103 3 -0.05274833362419429 1103 4 -0.03587108608508771 1103 5 -0.11986450475951926 1103 6 -0.038002480061791744 1103 7 -0.017900746126180422 1103 8 -0.0214457426670549 1103 9 0.028325475694479962 1103 10-11 -0.009267965062001338 1103 12-13 -0.0012925251498430157 1103 14-15 -0.02802142319550427 1103 16-17 0.05373576790085366 1103 18-19 -0.023369403720984394 1103 20-21 -0.08155445542173823 1103 22-23 0.07605827887017824 1103 24-25 0.07614674530175591 1103 26-27 0.05395644126787147 1103 28-29 -0.11345101980396777 1103 30-31 -0.17449373263563217 1103 32-33 -0.048161010943118754 1103 34-35 -0.04784131366849209 1103 36-37 -0.20522341675674483 1103 38-39 -0.09813800503085446 1103 40-41 -0.06197957124667397 1103 42-43 -0.09062480726274202 1103 44-45 -0.14038898382897003 1103 46-47 -0.11386713000178617 1103 48-49 -0.06594876629726087 1103 50-51 -0.06528677040039099 1103 52-53 -0.21369521693684845 1103 54-55 -0.21827639546796718 1103 56-57 -0.11365317472576919 1103 58-59 -0.22103725539867725 1103 60-61 -0.27394796275358857 1103 62-63 -0.15349875132870494 1103 64-65 0.04307427168635769 1103 66-67 -0.12369187915157198 1103 68-69 -0.36793231880135124 1103 70-71 -0.06705756252129191 1103 72-73 -0.1298489275936774 1103 74-75 0.023265664048160772 1103 76-77 -0.3697802618818251 1103 78-79 -0.39666904539786074 1103 80-81 -0.11138027399987394 1103 82-83 -0.2774258550982118 1103 84-85 -0.03862662158955743 1103 86-87 0.11973911655146097 1103 88-89 0.13069995226106457 1103 90-91 -0.07749054681011458 1103 92-93 0.058173168233853545 1103 94-95 0.016613715239049753 1103 96-97 0.08799412308415455 1103 98-99 0.4874360654355492 1103 100-101 1.173459475671546 1104 1 0.12900129462274634 1104 2 0.18104463729520148 1104 3 0.09873513196532002 1104 4 0.1081325044077488 1104 5 -0.0666803296656937 1104 6 0.017202302169089023 1104 7 0.11271966429770686 1104 8 0.09933041493265904 1104 9 0.13881429837569925 1104 10-11 0.1330759774137178 1104 12-13 0.09161481912684621 1104 14-15 0.20501827275851525 1104 16-17 0.1529832922751453 1104 18-19 0.09876608008837451 1104 20-21 0.19926314563249292 1104 22-23 0.26947268476775577 1104 24-25 0.1053347782655365 1104 26-27 -0.01623769362388572 1104 28-29 0.12448120009462116 1104 30-31 0.16384480500246212 1104 32-33 0.21563039670195394 1104 34-35 0.16634829491049885 1104 36-37 0.19365646406944848 1104 38-39 0.13438412520090282 1104 40-41 0.21714304981309596 1104 42-43 0.21587150197800753 1104 44-45 0.21591538683212264 1104 46-47 0.27535948166158164 1104 48-49 0.1425586008974804 1104 50-51 0.2524599088711952 1104 52-53 0.3340826881633703 1104 54-55 0.1315727274395755 1104 56-57 0.19217278186364695 1104 58-59 0.1395012230344932 1104 60-61 0.06219521769732239 1104 62-63 0.1873622030166544 1104 64-65 0.07313891965572594 1104 66-67 0.06578826332223997 1104 68-69 0.13660449807396446 1104 70-71 0.2089348363289858 1104 72-73 0.1963186987658858 1104 74-75 0.3884374061056235 1104 76-77 0.05156263973294273 1104 78-79 0.24002034832451002 1104 80-81 0.3692496856070768 1104 82-83 0.35357031567937725 1104 84-85 0.45713067637360183 1104 86-87 0.3412037702503383 1104 88-89 0.567267116440167 1104 90-91 0.5758908189140506 1104 92-93 0.6454546113063984 1104 94-95 0.8106574123007633 1104 96-97 0.9903752118820073 1104 98-99 1.030134282603072 1104 100-101 1.6803735616714874 1105 1 0.04236805457298942 1105 2 0.11788776540465307 1105 3 0.08178861455238717 1105 4 0.07404124196993678 1105 5 -0.019416648073658394 1105 6 0.04299909943276958 1105 7 0.07880330857631179 1105 8 0.06847298334061946 1105 9 0.10632051728117631 1105 10-11 0.10595766273710439 1105 12-13 0.1006885908930144 1105 14-15 0.0720328094500573 1105 16-17 0.19064070831991842 1105 18-19 0.13384653717793782 1105 20-21 0.25833303058274737 1105 22-23 0.16878860516576566 1105 24-25 0.20434947042971885 1105 26-27 0.14359400724676163 1105 28-29 0.14868634623889676 1105 30-31 0.20160716507709964 1105 32-33 0.2279418861298126 1105 34-35 0.08540729677616099 1105 36-37 0.09219890809929865 1105 38-39 0.051746531917324035 1105 40-41 0.1337902824001631 1105 42-43 -0.009002176504580461 1105 44-45 -0.018854124983796794 1105 46-47 -0.1990011371195166 1105 48-49 9.210853502210625E-4 1105 50-51 -0.04560056998452211 1105 52-53 -0.12360154691125302 1105 54-55 -0.26247850079425916 1105 56-57 -0.005411562041821583 1105 58-59 0.024739096168822527 1105 60-61 -0.03326498130764577 1105 62-63 -0.09504995494355484 1105 64-65 -0.12104446031940341 1105 66-67 -0.06729242697625892 1105 68-69 -0.13257888190116063 1105 70-71 0.04367325361754126 1105 72-73 -0.06123338456745131 1105 74-75 0.03550674690163902 1105 76-77 -0.21753523029193644 1105 78-79 -0.11182394582972321 1105 80-81 0.07568236595534117 1105 82-83 0.11675050846545076 1105 84-85 0.174428950627334 1105 86-87 0.12425142261351851 1105 88-89 0.23421557726106457 1105 90-91 0.42694803284439686 1105 92-93 0.5865265951372471 1105 94-95 0.5861102263554905 1105 96-97 0.7318630850163395 1105 98-99 1.0793141644438649 1105 100-101 1.7958353154028295 1106 1 -0.1211590710615269 1106 2 0.053292534863196295 1106 3 -0.14605614010073964 1106 4 0.027939560497856064 1106 5 -0.1505503198164675 1106 6 -0.0032083350003944133 1106 7 -0.18736367534923914 1106 8 -0.10241719220929468 1106 9 -0.020212900654527743 1106 10-11 -0.06169382837965287 1106 12-13 -0.06576326507144614 1106 14-15 -0.12049376098141806 1106 16-17 0.02208701225392673 1106 18-19 0.09027301243173724 1106 20-21 0.06805371057428289 1106 22-23 0.11305679467506735 1106 24-25 -0.01844227555167066 1106 26-27 0.05158562632459507 1106 28-29 -0.10051059221287062 1106 30-31 0.013535438131235367 1106 32-33 0.2223733317727934 1106 34-35 -0.21219805961131755 1106 36-37 0.039725610721973226 1106 38-39 -0.008856524343251237 1106 40-41 -0.01918798536633659 1106 42-43 -0.250684045159403 1106 44-45 -0.030166387039628262 1106 46-47 -0.0945271259096998 1106 48-49 -0.11225110690866558 1106 50-51 -0.3743931355513581 1106 52-53 -0.3286367097708123 1106 54-55 -0.5785972349313155 1106 56-57 -0.3601367365342085 1106 58-59 -0.512578479973314 1106 60-61 -0.2890813941672121 1106 62-63 -0.35318632338687195 1106 64-65 -0.42411592288285505 1106 66-67 -0.1678304451657695 1106 68-69 -0.37621718033602036 1106 70-71 -0.47742776689852917 1106 72-73 0.015946448342880615 1106 74-75 -0.2286244925230605 1106 76-77 -0.13240079184015485 1106 78-79 -0.0015004739600108508 1106 80-81 -0.28047377829170017 1106 82-83 -0.21545259009631224 1106 84-85 -0.02911575643696196 1106 86-87 0.0360038813698651 1106 88-89 0.15481162738796783 1106 90-91 0.2722314507801009 1106 92-93 0.42421562855518147 1106 94-95 0.440689990526387 1106 96-97 0.6965019386542401 1106 98-99 0.5381828721426842 1106 100-101 1.4977309372302408 1107 1 0.13868931660668693 1107 2 0.1431726255129533 1107 3 0.09363428242242122 1107 4 0.026287218203385976 1107 5 0.02871812930300166 1107 6 -0.009041927943890471 1107 7 0.18628864251132882 1107 8 0.06795120987491288 1107 9 0.1878111911680591 1107 10-11 0.10999553126898576 1107 12-13 0.15713648875703967 1107 14-15 0.12821276958843697 1107 16-17 0.2460450592525305 1107 18-19 0.2652746922621674 1107 20-21 0.11544936256228766 1107 22-23 0.30213698061702843 1107 24-25 0.4857710445873593 1107 26-27 0.21289821527925312 1107 28-29 0.28144008796573416 1107 30-31 0.3835486324356978 1107 32-33 0.2902797132958739 1107 34-35 0.45058056317207473 1107 36-37 0.40062343427258185 1107 38-39 0.4613387024949418 1107 40-41 0.38622992590196503 1107 42-43 0.41737840788169933 1107 44-45 0.483667780100447 1107 46-47 0.5036467457023903 1107 48-49 0.6058657115535837 1107 50-51 0.7914885921695074 1107 52-53 0.8117314479744167 1107 54-55 0.6980570926172902 1107 56-57 0.8460639757138892 1107 58-59 0.9523206996585856 1107 60-61 0.9153661859606999 1107 62-63 0.7328729165967687 1107 64-65 0.7093298063773474 1107 66-67 0.89136525202494 1107 68-69 0.6495101862396311 1107 70-71 0.6808550188100853 1107 72-73 0.7229733317382099 1107 74-75 0.9097828449762559 1107 76-77 0.7597159166695562 1107 78-79 0.9335068867515055 1107 80-81 0.81208335121406 1107 82-83 0.8178401218830231 1107 84-85 0.9069636415118119 1107 86-87 1.0215161074811476 1107 88-89 0.9853930930552899 1107 90-91 1.1830400906061307 1107 92-93 1.4671062807569832 1107 94-95 1.6125396976250457 1107 96-97 1.834422125329212 1107 98-99 2.021557132253733 1107 100-101 2.786443393020157 1108 1 0.2779517614123037 1108 2 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>>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 18.0 6 24.0 7 53.0 8 110.0 9 258.0 10 439.0 11 734.0 12 1260.0 13 1838.0 14 2499.0 15 3416.0 16 4908.0 17 7556.0 18 7149.0 19 4054.0 20 3152.0 21 3334.0 22 3366.0 23 3583.0 24 3990.0 25 4792.0 26 6082.0 27 7219.0 28 8970.0 29 11530.0 30 14786.0 31 19028.0 32 25418.0 33 34848.0 34 49808.0 35 76242.0 36 127123.0 37 211100.0 38 182443.0 39 17724.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.04077935822688 19.700221451443912 14.938602911279176 22.320396279050033 2 15.291295876125918 20.493083681446453 40.68258444835556 23.533035994072062 3 19.710859951323066 24.166943507896455 30.28466346708888 25.8375330736916 4 13.122863315827543 17.102897761944817 37.235734430331426 32.538504491896205 5 13.555731500987337 37.5885421821318 34.61628186458171 14.239444452299152 6 32.204056725357006 36.70502390258959 17.00582662435119 14.085092747702218 7 28.588274654773095 32.97473558717436 20.557640700137362 17.879349057915185 8 25.966529306294785 36.24146209866857 19.31279760432202 18.479210990714627 9 25.930127629697168 17.450068444576385 20.597812129651228 36.02199179607521 10-11 24.91606409522396 27.23746616057734 27.201594605483574 20.644875138715125 12-13 25.724148087061526 24.27602862780903 28.17718611021117 21.82263717491827 14-15 22.333253226083862 25.970475766378986 26.477919850340808 25.218351157196338 16-17 21.747527866720386 29.37108740878965 27.08726506782021 21.794119656669753 18-19 21.55615400383101 27.84816613301102 29.640094620798195 20.95558524235977 20-21 22.151081183443942 26.866750347377042 29.938217247454386 21.043951221724626 22-23 21.957493379324646 26.79258908460328 29.801874522887275 21.448043013184805 24-25 22.039649811246072 26.86313882263963 29.96749156070549 21.129719805408804 26-27 21.904442702200587 27.035141425107046 29.897866680646505 21.162549192045866 28-29 21.507805983723593 27.330721128698592 30.10569768482958 21.055775202748233 30-31 21.86737946747277 26.915573629511652 29.94381863633258 21.273228266683002 32-33 21.67266292989909 27.05251265812346 29.86015394728472 21.41467046469273 34-35 22.046752004448308 27.060111066345293 29.75701586359149 21.136121065614905 36-37 22.01895007669091 26.77673167134352 29.836003967664944 21.36831428430063 38-39 21.822864022656216 27.16749012206931 29.84436810694789 21.165277748326584 40-41 21.887244334179172 27.224978854042238 29.740346487414914 21.14743032436368 42-43 21.9305046144493 27.197916458025773 29.404640016400645 21.46693891112428 44-45 21.99509460901772 27.18339376718477 29.299167591434177 21.52234403236333 46-47 22.238184769727 27.672872622405052 28.728933560460945 21.360009047407 48-49 22.295614599310607 27.81877384074208 28.50810850291331 21.377503057034005 50-51 22.038034450829464 27.864364290073063 28.628976205790813 21.46862505330666 52-53 22.294214073791778 28.176622452543786 28.091508449847236 21.437655023817197 54-55 22.004283381751097 27.981992361538154 28.333286211421882 21.68043804528887 56-57 22.213681375771326 28.158228310373186 28.28080418254086 21.34728613131463 58-59 22.40723462706541 27.92526936062634 28.106040793438748 21.5614552188695 60-61 22.251673416880482 27.91024925135063 28.69306200536719 21.1450153264017 62-63 22.116786945345652 27.888573172082154 28.715562718086098 21.2790771644861 64-65 22.02401568217213 28.046961696954277 28.4734738979952 21.455548722878394 66-67 21.963817440290647 28.07329106497876 28.308193793573043 21.65469770115755 68-69 21.875169344369287 28.010618922745984 28.409682610365405 21.704529122519325 70-71 22.147475090179558 28.057446322252616 28.19781071599388 21.59726787157394 72-73 22.177174957281018 27.800766084926693 28.35091472519369 21.671144232598603 74-75 22.039742620119643 28.319679759489762 27.999839785501056 21.640737834889535 76-77 22.07225673902236 28.073349967368078 28.159877577274045 21.694515716335516 78-79 22.1372849768278 28.05721071269535 27.93834569105461 21.867158619422238 80-81 22.13510558842309 28.243106653378288 27.949949461749966 21.671838296448655 82-83 22.0502861478073 28.372574105096 27.988294917195024 21.588844829901678 84-85 21.976730008370033 28.2373508516996 27.872214725252586 21.913704414677778 86-87 21.884534824270613 28.306191112336283 27.95071519281108 21.858558870582026 88-89 21.962403782947053 28.24098616736289 27.70438540068939 22.09222464900066 90-91 21.975308932883348 28.068784362284887 27.869495628077622 22.086411076754146 92-93 22.259835622829023 27.78772926126876 27.881268624670945 22.071166491231274 94-95 21.8748748324227 27.789263743694498 28.004198562310485 22.331662861572315 96-97 21.760427489980707 28.046019258725213 28.10562847671353 22.087924774580557 98-99 21.933076086207436 28.291607739561144 27.563513160894114 22.21180301333731 100-101 21.430835320620975 28.87709607789539 27.482420751995928 22.209647849487705 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 117.0 1 82.0 2 69.0 3 102.0 4 97.0 5 88.5 6 169.5 7 391.5 8 665.0 9 855.0 10 911.0 11 912.0 12 975.5 13 1092.0 14 1204.5 15 1293.0 16 1391.5 17 1430.0 18 1481.0 19 1626.0 20 1741.0 21 1965.0 22 2365.5 23 2818.0 24 3403.5 25 4247.5 26 5314.5 27 6585.5 28 7722.5 29 8845.5 30 10281.5 31 11933.5 32 13611.0 33 15403.0 34 17554.0 35 19385.5 36 21416.0 37 23506.0 38 25192.0 39 26388.0 40 27225.5 41 28874.5 42 30351.5 43 31537.0 44 33217.0 45 34184.0 46 34121.0 47 34892.0 48 36886.0 49 35800.5 50 32767.0 51 30553.0 52 28256.5 53 26544.0 54 24744.5 55 22602.0 56 20270.0 57 17991.5 58 16019.5 59 13767.0 60 11085.0 61 8497.5 62 6446.0 63 4889.0 64 3624.0 65 2737.0 66 1954.5 67 1428.5 68 1024.0 69 607.0 70 394.0 71 270.0 72 213.0 73 169.0 74 117.0 75 80.5 76 56.0 77 35.5 78 18.5 79 11.0 80 9.5 81 7.0 82 4.5 83 2.5 84 2.0 85 1.0 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.043469963315591935 2 0.0 3 0.0 4 0.0 5 0.013429744764166612 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.004358776809422498 14-15 0.0 16-17 0.0 18-19 0.0 20-21 5.890238931652024E-5 22-23 7.068286717982429E-4 24-25 6.479262824817225E-4 26-27 0.0024149979619773297 28-29 0.0030629242444590522 30-31 4.712191145321619E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.013547549542799653 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 5.890238931652024E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 5.890238931652024E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0 84-85 5.890238931652024E-5 86-87 0.0 88-89 0.0 90-91 0.011014746802189283 92-93 0.00253280274061037 94-95 0.0 96-97 0.0 98-99 2.9451194658260116E-4 100-101 0.015785840336827422 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 848862.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.75712662703756 #Duplication Level Percentage of deduplicated Percentage of total 1 81.82661314757553 53.80682962206741 2 11.073606749589064 14.563371225014917 3 3.1691688032421763 6.251863028917587 4 1.3593927577525637 3.575590468296525 5 0.686151990401102 2.255969165909956 6 0.42637933402112116 1.6822487915027284 7 0.2820949301640193 1.298482643054851 8 0.1912913755240592 1.0063016962396605 9 0.14473871918763112 0.8565842056910663 >10 0.7754738558838009 9.132636351361977 >50 0.04478366063954619 1.9970934162510106 >100 0.01921692554031417 2.152973143116588 >500 3.6258349306704583E-4 0.17583805891531454 >1k 7.251669861340917E-4 1.2442181836604325 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3545 0.41761794025412846 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3147 0.37073163835817835 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2080 0.2450339395567242 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1665 0.1961449564240124 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.1780477863304048E-4 0.0 14-15 0.0 0.0 0.0 2.3560955726608095E-4 0.0 16-17 0.0 0.0 0.0 2.3560955726608095E-4 0.0 18-19 0.0 0.0 0.0 3.534143358991214E-4 0.0 20-21 0.0 0.0 0.0 3.534143358991214E-4 0.0 22-23 0.0 0.0 0.0 5.890238931652023E-4 0.0 24-25 0.0 0.0 0.0 8.246334504312833E-4 0.0 26-27 0.0 0.0 0.0 0.001236950175646925 0.0 28-29 0.0 0.0 0.0 0.0022971931833442893 0.0 30-31 0.0 0.0 0.0 0.005536824595752903 0.0 32-33 0.0 0.0 0.0 0.008717553618844995 0.0 34-35 0.0 0.0 0.0 0.011014746802189284 0.0 36-37 0.0 0.0 0.0 0.015668035558194383 0.0 38-39 0.0 0.0 0.0 0.02303083422275941 0.0 40-41 0.0 0.0 0.0 0.03168948545228789 0.0 42-43 0.0 0.0 0.0 0.039876917567284204 0.0 44-45 0.0 0.0 0.0 0.043882280040807575 0.0 46-47 0.0 0.0 0.0 0.050125933308358725 0.0 48-49 0.0 0.0 0.0 0.05931470604173587 0.0 50-51 0.0 0.0 0.0 0.06826786921784696 0.0 52-53 0.0 0.0 0.0 0.08599748840211954 0.0 54-55 0.0 0.0 0.0 0.09907381883038704 0.0 56-57 0.0 0.0 0.0 0.14018768657331815 0.0 58-59 0.0 0.0 0.0 0.2049214124321739 0.0 60-61 0.0 0.0 0.0 0.2631758754662124 0.0 62-63 0.0 0.0 0.0 0.2955721895902985 0.0 64-65 0.0 0.0 0.0 0.3511171427157771 0.0 66-67 0.0 0.0 0.0 0.46067558684450477 0.0 68-69 0.0 0.0 0.0 0.6224804502969858 0.0 70-71 0.0 0.0 0.0 0.7825182420699713 0.0 72-73 0.0 0.0 0.0 0.961817115149459 0.0 74-75 0.0 0.0 0.0 1.1782833958876708 0.0 76-77 0.0 0.0 0.0 1.333196679790119 0.0 78-79 0.0 0.0 0.0 1.5045437303118763 0.0 80-81 0.0 0.0 0.0 1.684196017727263 0.0 82-83 0.0 0.0 0.0 1.8652030600969298 0.0 84-85 0.0 0.0 0.0 2.077840685529568 0.0 86-87 0.0 0.0 0.0 2.3975628547396397 0.0 88-89 0.0 0.0 0.0 2.8929908512808913 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4915 0.0 26.400595 1 GTACATG 2725 0.0 25.11716 1 GGTATCA 3620 0.0 25.078373 1 TACATGG 2840 0.0 23.917223 2 ACATGGG 2770 0.0 23.492752 3 ATCAACG 5690 0.0 22.706472 3 TCAACGC 5765 0.0 22.411074 4 CAACGCA 5780 0.0 22.352913 5 AACGCAG 5950 0.0 21.792807 6 GAGTACT 3555 0.0 21.781723 12-13 TATCAAC 5925 0.0 21.645542 2 ACTATAC 225 7.146264E-8 21.111084 3 GTACTTT 3635 0.0 21.037249 14-15 CATGGGG 1300 0.0 20.826897 4 AGTACTT 3650 0.0 20.694193 12-13 GGACCGA 140 4.8522677E-4 20.355919 6 ATATAGG 305 2.055458E-10 20.259005 1 AGAGTAC 4985 0.0 20.202957 10-11 CCTACAC 290 2.4356268E-9 19.655148 3 GCACCGT 170 7.9544065E-5 19.557648 6 >>END_MODULE