FastQCFastQC Report
Thu 26 May 2016
SRR937675_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937675_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397513
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26840.67519804383756No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20930.5265236608614058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13210.3323161758231806No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5680.14288840868097397No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4870.12251171659794773No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA14550.038.552251
GTATCAA25000.037.6492081
GTATAGG1404.4929038E-1033.9549141
TATAGGG702.0446147E-433.9463542
GGTCGGG600.003932887731.6912561
ATCAACG30300.031.0558743
TCAACGC30200.031.0013394
CAACGCA30450.030.902895
TATCAAC30950.030.2500952
AACGCAG31250.030.0928086
GACCTAG1004.9189483E-528.5221271
ACGCAGA34600.026.904667
CGCAGAG34650.026.8658378
GTACATG19600.025.9512561
TACATGG19800.025.6826462
ACATGGG19550.025.2817843
GCAGAGT36150.024.831399
GGACCGA1151.2806099E-424.779986
GTCCTAC1000.001644297923.768441
GAGTACT20750.023.3469212-13
Produced by FastQC (version 0.11.5)