FastQCFastQC Report
Thu 26 May 2016
SRR937675_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937675_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences397513
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33510.8429912983977883No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.7567048121696649No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19940.5016188149821516No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7210.18137771594891236No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4960.12477579349606176No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4800.12075076789941461No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATCC250.00161470738.00387694-95
GCCGTAT250.001615705337.99909692-93
GGTATCA19800.036.948211
GTATCAA30950.033.1536251
CGCCGTA300.003948255431.66989590-91
GTATAGG2051.8189894E-1230.1250231
GTCCTAC1004.941633E-528.5029071
GTGTAGC1205.3658096E-627.7111591
CCGCCGT350.0083858727.14562490-91
CTAGACT1754.942194E-927.142214
CCGTATC350.00839101727.1422192-93
GGGCGTA700.00838605927.142219
TCAACGC38450.026.6833694
ATCAACG38600.026.5796783
CAACGCA38650.026.5452925
TATCAAC38950.026.3408362
TACCTGG1658.3831765E-825.9084742
AACGCAG39700.025.8432126
TCTAGAC1301.000142E-525.5763133
GGTAAGG950.001225313624.9994033
Produced by FastQC (version 0.11.5)