FastQCFastQC Report
Thu 26 May 2016
SRR937674_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937674_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359957
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24360.6767475003958806No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20140.5595112749578423No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12300.34170748172698406No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5360.14890667496395404No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4240.11779184735954572No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA13100.045.3542941
GTATCAA23050.037.5301361
ATCAACG27400.031.2205873
TCAACGC27700.030.882464
CAACGCA28000.030.7213085
TATCAAC28100.030.4470942
AACGCAG28750.030.0726096
AGTAGCG650.0058200229.250032
GTACATG19850.028.255381
TACATGG20400.027.7265912
ACATGGG19850.026.575513
ACGCAGA32550.026.4158847
AGGACCG908.9084555E-426.4025965
ACCGTCC458.960819E-426.38053162-63
CGCAGAG32750.026.2545648
GTATAGA1109.416558E-525.9261631
GAGTACT19400.025.82660312-13
GTACTTT20000.025.05180414-15
TACCTGG2351.72804E-1024.2713012
GCAGAGT35250.023.9882439
Produced by FastQC (version 0.11.5)