##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937674_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 359957 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.937959256244497 33.0 31.0 34.0 30.0 34.0 2 32.072105834863606 33.0 31.0 34.0 30.0 34.0 3 32.10082037576711 34.0 31.0 34.0 30.0 34.0 4 35.66262914737038 37.0 35.0 37.0 35.0 37.0 5 35.64955258544771 37.0 35.0 37.0 35.0 37.0 6 35.72835366446548 37.0 35.0 37.0 35.0 37.0 7 35.68238706289918 37.0 35.0 37.0 35.0 37.0 8 35.66833538450426 37.0 35.0 37.0 35.0 37.0 9 37.329731051208896 39.0 37.0 39.0 35.0 39.0 10-11 37.22890928638699 39.0 37.0 39.0 34.0 39.0 12-13 37.113981670032814 39.0 37.0 39.0 34.0 39.0 14-15 38.29602980355987 40.0 38.0 41.0 33.5 41.0 16-17 38.247974063568705 40.0 38.0 41.0 34.0 41.0 18-19 38.23416130260003 40.0 38.0 41.0 33.5 41.0 20-21 37.97071177946255 40.0 38.0 41.0 33.0 41.0 22-23 37.934257425192456 40.0 38.0 41.0 33.0 41.0 24-25 37.822378784132546 40.0 38.0 41.0 33.0 41.0 26-27 37.909050247668475 40.0 38.0 41.0 33.0 41.0 28-29 37.84645527104627 40.0 38.0 41.0 33.0 41.0 30-31 37.620158796745166 40.0 37.0 41.0 33.0 41.0 32-33 37.3681064682726 40.0 37.0 41.0 32.5 41.0 34-35 37.100799539945044 39.5 36.0 41.0 31.5 41.0 36-37 37.156378400753425 40.0 36.5 41.0 32.0 41.0 38-39 37.123510585986665 40.0 37.0 41.0 32.0 41.0 40-41 36.93865239459157 40.0 36.0 41.0 31.0 41.0 42-43 36.680688248874176 40.0 36.0 41.0 31.0 41.0 44-45 36.414809268884895 39.5 36.0 41.0 30.0 41.0 46-47 35.993163072255854 39.0 35.5 41.0 29.5 41.0 48-49 35.60275394005395 39.0 35.0 41.0 27.5 41.0 50-51 34.6961442616757 38.5 34.0 40.0 25.0 40.5 52-53 34.78714957619938 38.5 35.0 40.0 26.0 40.5 54-55 35.08849945965768 39.0 35.0 40.0 26.0 41.0 56-57 34.86837455584973 39.0 35.0 40.0 24.5 41.0 58-59 34.65469208822165 38.5 34.5 40.0 23.5 41.0 60-61 34.36894129020966 38.0 34.0 40.0 23.0 41.0 62-63 34.023205549551754 38.0 34.0 40.0 22.0 41.0 64-65 33.66637820628575 37.5 34.0 40.0 20.5 41.0 66-67 33.25531521820662 37.0 33.0 40.0 18.5 41.0 68-69 32.8024180666024 36.0 32.5 39.0 16.5 41.0 70-71 32.37095680872993 36.0 32.0 39.0 12.0 40.5 72-73 31.900263086979834 35.5 31.5 38.5 8.0 40.0 74-75 31.414156413127124 35.0 31.0 38.0 7.0 40.0 76-77 30.967751703675717 35.0 31.0 37.0 2.0 39.0 78-79 30.434174081904228 35.0 30.5 36.5 2.0 39.0 80-81 29.89169539695019 34.0 29.5 36.0 2.0 38.5 82-83 29.37212083665549 34.0 29.0 35.5 2.0 37.0 84-85 28.917716560589184 34.0 29.0 35.0 2.0 37.0 86-87 28.436028470067257 34.0 28.5 35.0 2.0 36.0 88-89 27.958126942940407 34.0 27.0 35.0 2.0 36.0 90-91 27.45309995360557 33.5 26.0 35.0 2.0 35.5 92-93 26.93095286381429 33.0 25.5 35.0 2.0 35.0 94-95 26.339964217948257 33.0 25.0 35.0 2.0 35.0 96-97 25.61070211164111 32.0 22.0 34.5 2.0 35.0 98-99 24.744461143969975 32.0 10.0 34.0 2.0 35.0 100-101 22.25160088566134 29.5 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.2531522971151823 1101 2 0.29693921604477325 1101 3 0.4232684393340911 1101 4 0.27360926844860245 1101 5 0.31924037866041743 1101 6 0.3189147929569316 1101 7 0.22537307127930717 1101 8 0.15465381095928876 1101 9 0.3542649954349386 1101 10-11 0.17375728631980536 1101 12-13 0.3303430367997535 1101 14-15 0.5935036234414852 1101 16-17 0.5573988162906858 1101 18-19 0.45207026183324217 1101 20-21 0.2730390680504087 1101 22-23 0.3388368446094816 1101 24-25 0.41729429945819163 1101 26-27 0.5397854262529762 1101 28-29 0.3842441049966041 1101 30-31 0.4083176624709708 1101 32-33 0.41155383497955 1101 34-35 0.5078667736251745 1101 36-37 0.4033548726818168 1101 38-39 0.47011436306053156 1101 40-41 0.5289931571200626 1101 42-43 0.20792435684651167 1101 44-45 0.4145675640100208 1101 46-47 0.8173517214837176 1101 48-49 1.0135581572237768 1101 50-51 1.0080589452618653 1101 52-53 0.8924191321331207 1101 54-55 1.0133308048458076 1101 56-57 1.3152147241248642 1101 58-59 1.2168745015083644 1101 60-61 1.2408255119077651 1101 62-63 1.386547955629979 1101 64-65 1.3769471184703406 1101 66-67 1.4730327650389583 1101 68-69 1.6755054299657957 1101 70-71 1.8345963553935682 1101 72-73 1.6430602545971418 1101 74-75 1.633515044780328 1101 76-77 1.2593130535193104 1101 78-79 1.5177752857292326 1101 80-81 1.4526579182805683 1101 82-83 1.805884657408317 1101 84-85 1.9701660894559367 1101 86-87 1.9083407250655853 1101 88-89 1.9737682881385687 1101 90-91 2.0144602027797767 1101 92-93 2.1544369265492556 1101 94-95 2.2249875790586984 1101 96-97 2.162383414043685 1101 98-99 2.4026080946435506 1101 100-101 2.8763433782951893 1102 1 0.20060310212412347 1102 2 0.18887421902628532 1102 3 0.16486056813551997 1102 4 0.15642829051181195 1102 5 0.15589810078325428 1102 6 0.30727943219366693 1102 7 0.22789245112426926 1102 8 0.1988622188304845 1102 9 0.214938817736666 1102 10-11 0.16399282597394915 1102 12-13 0.18494947687131003 1102 14-15 0.40797514997696993 1102 16-17 0.2554720124743426 1102 18-19 0.25866313601332536 1102 20-21 0.39292577049525335 1102 22-23 0.3974273335778875 1102 24-25 0.412989737740844 1102 26-27 0.440258741697221 1102 28-29 0.3432221908642319 1102 30-31 0.375852695267632 1102 32-33 0.2215009130952339 1102 34-35 0.4388409835237965 1102 36-37 0.7461545149000628 1102 38-39 0.7498624250760315 1102 40-41 0.6488984940312079 1102 42-43 0.7064186919687501 1102 44-45 0.7572442187506354 1102 46-47 0.8400559552344617 1102 48-49 0.9207547881122764 1102 50-51 0.7963341390603134 1102 52-53 0.7488890188355697 1102 54-55 0.9226815800396082 1102 56-57 0.8806925118410263 1102 58-59 0.8016128736013783 1102 60-61 0.7444666866245271 1102 62-63 0.6990255942704096 1102 64-65 0.6830592234196544 1102 66-67 0.8759061997437882 1102 68-69 1.085843831873973 1102 70-71 1.1029960274269648 1102 72-73 1.2083107018243346 1102 74-75 1.1394296243867679 1102 76-77 1.2984379789814433 1102 78-79 1.1641229303326917 1102 80-81 1.2620236010474137 1102 82-83 1.1114712704077228 1102 84-85 1.2019453082991092 1102 86-87 1.3225103732468604 1102 88-89 1.2943929154492437 1102 90-91 1.3744147346819844 1102 92-93 1.4279163540984499 1102 94-95 1.2554102087545829 1102 96-97 1.1511095619268126 1102 98-99 1.27842055737462 1102 100-101 1.025952054502703 1103 1 0.11205463073661548 1103 2 0.14696802618594162 1103 3 0.11462539698318253 1103 4 0.08120074064681404 1103 5 0.08749160309141502 1103 6 0.16093438385293268 1103 7 0.21263898888230415 1103 8 0.1870220045634312 1103 9 0.04737937169537787 1103 10-11 0.13749252112245358 1103 12-13 0.2747826795540007 1103 14-15 0.4298836292035162 1103 16-17 0.38388253856092547 1103 18-19 0.3129893053365578 1103 20-21 0.4490402204560553 1103 22-23 0.6358838051395921 1103 24-25 0.5085144089367333 1103 26-27 0.36245900735352166 1103 28-29 0.33934349998364866 1103 30-31 0.5245521770200909 1103 32-33 0.5715221438242608 1103 34-35 0.6768238465148286 1103 36-37 0.7233995284006269 1103 38-39 0.9081293443339433 1103 40-41 0.9866494521359002 1103 42-43 1.249533403230835 1103 44-45 1.2740028852430996 1103 46-47 1.2377565039671552 1103 48-49 1.4104034467657058 1103 50-51 1.2613360988199176 1103 52-53 1.2692269684915232 1103 54-55 1.3890798685162196 1103 56-57 1.5931821686653436 1103 58-59 1.3618183217330753 1103 60-61 1.3178134116484799 1103 62-63 1.3938801365576694 1103 64-65 1.6047777148402602 1103 66-67 1.6789748566552092 1103 68-69 1.404538849729562 1103 70-71 1.5774946555952347 1103 72-73 1.5202282177201631 1103 74-75 1.140782402890384 1103 76-77 1.437619017218534 1103 78-79 1.7508205176508689 1103 80-81 1.9170269761889465 1103 82-83 1.6643548389122067 1103 84-85 2.0197339373383905 1103 86-87 1.8968886939506326 1103 88-89 2.214080878170261 1103 90-91 1.9488883214775576 1103 92-93 2.0572387127780054 1103 94-95 1.8698737790010789 1103 96-97 2.021321760341582 1103 98-99 1.923970814320878 1103 100-101 2.4486640351664057 1104 1 0.036211934107402755 1104 2 -0.024380288420800866 1104 3 -0.24492516481457116 1104 4 -0.28584045785505907 1104 5 -0.1831076478449134 1104 6 0.07947370969562684 1104 7 -0.031743033589606284 1104 8 -0.006798220155666002 1104 9 0.21030072000998956 1104 10-11 0.17447753984905034 1104 12-13 0.14229204284988128 1104 14-15 0.18831059549564344 1104 16-17 0.12732823144482097 1104 18-19 0.20484323792082648 1104 20-21 0.044545838433577956 1104 22-23 0.36153923585120396 1104 24-25 0.18641703065958382 1104 26-27 0.2678897189639997 1104 28-29 0.28597271346679065 1104 30-31 0.14767951784406108 1104 32-33 0.3552300089928053 1104 34-35 0.4923669176758736 1104 36-37 0.3591673186628057 1104 38-39 0.5176799061316899 1104 40-41 0.38786668060031815 1104 42-43 0.6624547515454466 1104 44-45 0.7187594395502188 1104 46-47 0.687194706214342 1104 48-49 0.9604970797244974 1104 50-51 0.5071226156738575 1104 52-53 0.5407625490158665 1104 54-55 0.49582144154993557 1104 56-57 0.7429949027477463 1104 58-59 0.86462731049712 1104 60-61 0.9549856962926739 1104 62-63 1.1569887507898784 1104 64-65 1.1464443815069316 1104 66-67 1.3404917105877914 1104 68-69 1.3712991493550248 1104 70-71 0.9749665657076001 1104 72-73 0.950471663413051 1104 74-75 0.7493966351001227 1104 76-77 0.7203905527990564 1104 78-79 0.6787231393737194 1104 80-81 0.6426824069005583 1104 82-83 0.9110776853541545 1104 84-85 0.49632569763801726 1104 86-87 0.3439860722277892 1104 88-89 0.6443243238631418 1104 90-91 0.6984201566835502 1104 92-93 0.3910402109053486 1104 94-95 0.10629699997486242 1104 96-97 0.384149475697388 1104 98-99 0.7151693161935384 1104 100-101 0.3784393160652826 1105 1 0.4903317842946713 1105 2 0.3587396020387068 1105 3 0.6458766554906923 1105 4 0.3641937735492391 1105 5 0.22417558682143124 1105 6 0.24393547120342163 1105 7 0.49490712145851745 1105 8 0.514717224248507 1105 9 0.5576286568631161 1105 10-11 0.5453798393939735 1105 12-13 0.6026893285008796 1105 14-15 0.6585091376405501 1105 16-17 0.7139538204263403 1105 18-19 0.4552409262942305 1105 20-21 0.6624931623432104 1105 22-23 0.6094048071129308 1105 24-25 0.7215746964805305 1105 26-27 0.8367250457732354 1105 28-29 0.741371207284999 1105 30-31 0.6759355284759394 1105 32-33 0.44305369712457576 1105 34-35 0.3442053454476124 1105 36-37 0.5397279082484019 1105 38-39 0.5351681265013895 1105 40-41 0.4768632977321303 1105 42-43 0.5535888178335497 1105 44-45 0.2528498910020289 1105 46-47 0.0704052092146199 1105 48-49 0.24919366866574677 1105 50-51 0.3006216692762038 1105 52-53 0.13002596973996106 1105 54-55 0.3682591202580028 1105 56-57 0.7515326177008248 1105 58-59 0.8132902721176762 1105 60-61 0.6710915720125357 1105 62-63 0.8874164419823387 1105 64-65 0.7388440029478716 1105 66-67 0.7945863906248434 1105 68-69 1.1475415479696665 1105 70-71 1.4851202043045113 1105 72-73 1.3140142110349693 1105 74-75 1.1430477164097788 1105 76-77 0.8481743714128847 1105 78-79 1.0972624652164136 1105 80-81 1.072598640640642 1105 82-83 1.1743020822773538 1105 84-85 1.0205796543856316 1105 86-87 1.1710923103978175 1105 88-89 1.2178060604021503 1105 90-91 1.0320504977894274 1105 92-93 1.3675262565740702 1105 94-95 1.9327488142392895 1105 96-97 1.7821449249342294 1105 98-99 1.798472445346615 1105 100-101 1.2225454736908326 1106 1 0.11803741200029805 1106 2 0.0030164721220273805 1106 3 -0.013761197695547622 1106 4 0.02550737034670192 1106 5 0.10305656247660266 1106 6 -0.13115180710966712 1106 7 0.06433411024034541 1106 8 0.14251594882142626 1106 9 0.20288304405298874 1106 10-11 -0.29281780525528234 1106 12-13 -0.13196132550461215 1106 14-15 -0.25565555071768387 1106 16-17 -0.1562458723540061 1106 18-19 0.07244988221286519 1106 20-21 -0.038665893654552974 1106 22-23 -0.5279130721404286 1106 24-25 -0.17690316973927622 1106 26-27 -0.4533880669205246 1106 28-29 -0.1887828680303656 1106 30-31 -0.04344723929392558 1106 32-33 -0.08203590861018739 1106 34-35 -0.5855065197481295 1106 36-37 -0.25178165745471404 1106 38-39 -0.5175479746018112 1106 40-41 -0.5081946550677472 1106 42-43 -0.23961976569761845 1106 44-45 -0.6190488196063555 1106 46-47 -0.2943948652628663 1106 48-49 -0.5769013832357572 1106 50-51 -0.6753826131796856 1106 52-53 -0.3108109710969842 1106 54-55 -0.02249170597669803 1106 56-57 -0.16456383065368385 1106 58-59 -0.28918610455225036 1106 60-61 -0.3605148144325554 1106 62-63 -0.24936613028410193 1106 64-65 0.041082982606205576 1106 66-67 -0.04295957595338251 1106 68-69 -0.2703237408633683 1106 70-71 -0.49688882609161666 1106 72-73 -0.3343123727754822 1106 74-75 -0.2786810924158054 1106 76-77 -0.28807289386663726 1106 78-79 0.09509796305190932 1106 80-81 -0.0882261976035501 1106 82-83 -0.5513414498177873 1106 84-85 -0.16081959152974434 1106 86-87 -0.01440319767833742 1106 88-89 0.6204965598668402 1106 90-91 0.8748331631527364 1106 92-93 0.4719111190234493 1106 94-95 0.1680278720989925 1106 96-97 -0.15101152435125087 1106 98-99 0.06268135471681191 1106 100-101 -0.2341877572359472 1107 1 0.5787671584443252 1107 2 0.7500789334702027 1107 3 0.7221695382351889 1107 4 0.6352785515464561 1107 5 0.6905642928414721 1107 6 0.7148593246157517 1107 7 0.7872778331840706 1107 8 0.8586569506767106 1107 9 0.8936684491241067 1107 10-11 0.8126243716154633 1107 12-13 0.947506825392118 1107 14-15 1.1076715983237477 1107 16-17 1.25531875349634 1107 18-19 1.114812281718116 1107 20-21 1.3688789424617056 1107 22-23 1.026199481972121 1107 24-25 1.0837647337857703 1107 26-27 1.3236682101357573 1107 28-29 1.010042116508906 1107 30-31 1.1717661187765742 1107 32-33 1.037011710437426 1107 34-35 1.2669981184708021 1107 36-37 1.4083915027184446 1107 38-39 1.1670531385707363 1107 40-41 0.937692790819689 1107 42-43 1.6011347179140145 1107 44-45 0.7520086943082944 1107 46-47 1.3731994451885825 1107 48-49 1.2864257658056815 1107 50-51 0.8252358160101707 1107 52-53 0.3091642917354278 1107 54-55 -0.08649912712697727 1107 56-57 -0.4666664898992039 1107 58-59 -0.5912887637977633 1107 60-61 0.13144560609929812 1107 62-63 -0.03786334735275432 1107 64-65 -0.12341918189596157 1107 66-67 -0.09181548195214617 1107 68-69 -0.1089137223104899 1107 70-71 0.1895675746504999 1107 72-73 0.2776233105887762 1107 74-75 0.7355427789509257 1107 76-77 0.6692554920331766 1107 78-79 1.076854301951105 1107 80-81 0.9779457257112334 1107 82-83 1.2680772267437455 1107 84-85 1.0274920967819448 1107 86-87 0.6058812797489956 1107 88-89 1.0821539500956625 1107 90-91 1.594993954742101 1107 92-93 0.6766111808663666 1107 94-95 0.7190482802622569 1107 96-97 0.10161679970564208 1107 98-99 0.6273072050569475 1107 100-101 1.054927889022558 1108 1 0.597858666015096 1108 2 0.34619479917132523 1108 3 0.48100210351936923 1108 4 0.5989607986388421 1108 5 0.6955017516959856 1108 6 0.47511826690234216 1108 7 0.6221472648276958 1108 8 0.3677638192305821 1108 9 0.29239568195271914 1108 10-11 0.6403619182470166 1108 12-13 0.4261660310098492 1108 14-15 0.5008030444505849 1108 16-17 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-0.6802975389950632 2316 70-71 -0.26538677551061696 2316 72-73 -0.372931648236861 2316 74-75 -0.4830841455030672 2316 76-77 -0.2636795240785297 2316 78-79 -0.23497437688885014 2316 80-81 -0.2510195446074235 2316 82-83 0.25418087873085327 2316 84-85 0.17536177096740957 2316 86-87 0.17563154827746175 2316 88-89 0.010121189623049531 2316 90-91 0.14227734178112428 2316 92-93 0.619011825334681 2316 94-95 0.7988236114473608 2316 96-97 0.8935743722083274 2316 98-99 0.9085460163709485 2316 100-101 1.8646530948943791 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1443.0 3 290.0 4 252.0 5 308.0 6 322.0 7 368.0 8 442.0 9 475.0 10 620.0 11 860.0 12 1145.0 13 1617.0 14 1771.0 15 2281.0 16 3324.0 17 4717.0 18 4186.0 19 2484.0 20 2246.0 21 2396.0 22 2574.0 23 2839.0 24 3032.0 25 3352.0 26 3886.0 27 4403.0 28 5287.0 29 6449.0 30 7791.0 31 9675.0 32 12474.0 33 16467.0 34 23080.0 35 33451.0 36 50517.0 37 72521.0 38 62022.0 39 8589.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.21227384925698 19.772275186520385 15.473251945996847 21.542199018225787 2 14.56252280050015 20.229856889440896 42.56891668732056 22.638703622738397 3 18.590086326928432 25.82511835143414 30.782511835143417 24.802283486494012 4 11.917824241681613 17.057847188958572 38.80067059518107 32.22365797417874 5 12.56572351565111 37.835022836136794 35.381530578144144 14.21772307006795 6 29.880233020950175 37.180368569448426 18.307397274000017 14.632001135601383 7 26.956141791231698 33.91155998742058 21.656364714166042 17.475933507181683 8 25.27887248680783 35.33748580589139 20.588415380847415 18.795226326453367 9 26.08664187579327 17.11349618116636 21.64376850965285 35.15609343338752 10-11 23.92821257894195 27.52161944762207 27.881085383945248 20.669082589490735 12-13 24.97303766377445 25.146849059623293 28.888177624392043 20.991935652210216 14-15 21.96312990813789 26.156974130816877 27.752349435064023 24.12754652598121 16-17 20.51012684295184 29.72126831820383 28.387580633491215 21.381024205353118 18-19 20.293440720465988 27.580895183442195 31.91529626547702 20.210367830614796 20-21 21.15586363497227 26.639377335756166 31.95745758419853 20.24730144507303 22-23 20.7938564206888 26.68892076881012 31.96854184610718 20.5486809643939 24-25 20.641818229886304 26.860467219627175 32.128341382846536 20.369373167639985 26-27 20.3741170970094 27.034920935306655 32.055292408564924 20.535669559119018 28-29 20.10467881749954 27.058263600801023 32.35816799544657 20.47888958625287 30-31 20.222093888939142 26.882405738817027 32.246891590072565 20.64860878217126 32-33 20.05152013627368 27.145906918617115 32.14887120522605 20.653701739883154 34-35 20.481552144259684 27.001237139799585 32.048043885760094 20.469166830180644 36-37 20.501004569259624 26.759248437491305 31.98231290245382 20.75743409079525 38-39 20.128471613533343 27.21597533523105 32.08548635284755 20.570066698388054 40-41 20.24155187549825 27.08227539028534 32.10516869814919 20.57100403606722 42-43 20.1939359196094 27.16076867527213 31.68844468624207 20.956850718876403 44-45 20.20813273297007 27.375479353700417 31.4232004408106 20.99318747251891 46-47 20.49154551728744 27.548033038715854 30.948332479907386 21.01208896408932 48-49 20.577032535316523 27.762245450357863 30.62458511522142 21.036136899104196 50-51 20.60421438770029 27.816851427664886 30.474570893393416 21.10436329124141 52-53 20.77372750427993 28.099155149136358 30.189569501858116 20.9375478447256 54-55 20.496915152071733 27.971792495623887 30.268244016062916 21.263048336241468 56-57 20.565979724230615 28.20340878894919 30.132208478426907 21.098403008393284 58-59 20.639717288923208 27.84815409979757 30.215859367934833 21.296269243344394 60-61 20.608656578963842 27.844174344663337 30.519497683037272 21.027671393335552 62-63 20.57946658245212 27.763255096348217 30.70009835851191 20.95717996268776 64-65 20.412047284297046 27.616962135556296 30.669406414292688 21.30158416585397 66-67 20.280132776480865 27.956377416868623 30.29443435048873 21.469055456161783 68-69 20.35632897127609 27.79121114564522 30.542841004265597 21.30961887881309 70-71 20.459528645760873 27.7901444113637 30.417095634269174 21.333231308606248 72-73 20.413640164220652 27.713835353629506 30.26618381750416 21.606340664645675 74-75 20.121076526431054 27.9797806931499 30.24700891447144 21.652133865947608 76-77 20.28718944736692 27.762295846034625 30.29054244885977 21.659972257738687 78-79 20.29880400483073 27.765147509781112 30.11971082406238 21.81633766132578 80-81 20.345340436218034 27.8995059608859 29.987102949626376 21.76805065326969 82-83 20.57504458410886 27.6728903578692 30.094899466659992 21.657165591361952 84-85 20.152713806980767 27.564021417214175 29.97422311128299 22.30904166452207 86-87 20.40533284367323 27.434598381339498 30.246666277164312 21.913402497822958 88-89 20.310615429774177 27.275395746705417 30.122518366625812 22.291470456894597 90-91 20.146241908490296 27.450268430139303 30.368011530183956 22.035478131186448 92-93 20.165047584732264 27.454741987944182 30.28005503441042 22.100155392913138 94-95 19.780024095513948 27.350634246236122 30.326124155891854 22.543217502358075 96-97 19.84958075435001 27.62096353460185 30.119047122181552 22.41040858886658 98-99 19.8909732956925 27.75923408601088 29.840981852535677 22.508810765760938 100-101 20.111710082426182 27.37745626543317 30.146836851806768 22.363996800333876 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 428.0 1 255.5 2 95.5 3 111.5 4 107.0 5 107.5 6 160.5 7 345.5 8 623.5 9 784.5 10 751.0 11 688.0 12 674.5 13 705.5 14 784.0 15 810.0 16 824.0 17 881.0 18 921.5 19 969.0 20 1048.5 21 1202.5 22 1376.0 23 1584.0 24 1914.5 25 2366.5 26 3011.0 27 3671.0 28 4032.5 29 4596.0 30 5478.5 31 6253.0 32 7086.5 33 8118.0 34 9337.0 35 10259.5 36 11288.0 37 12184.5 38 12575.5 39 12711.5 40 12531.0 41 12727.5 42 12902.5 43 13175.0 44 13573.0 45 13691.0 46 13420.5 47 13189.0 48 13736.5 49 13371.0 50 12137.0 51 11212.0 52 10266.0 53 9561.5 54 8840.0 55 7893.0 56 6946.0 57 5915.0 58 5066.0 59 4371.5 60 3632.5 61 2857.0 62 2189.0 63 1622.0 64 1150.5 65 876.0 66 626.0 67 460.0 68 324.0 69 206.0 70 154.0 71 112.5 72 82.5 73 63.5 74 42.5 75 29.0 76 25.0 77 20.5 78 14.5 79 9.0 80 5.5 81 3.5 82 4.5 83 4.0 84 1.5 85 2.0 86 2.0 87 0.5 88 1.0 89 0.5 90 0.0 91 1.0 92 1.0 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.28197812516495024 2 0.24002867009114978 3 0.23808399336587424 4 0.2425289687379326 5 0.24364021258094717 6 0.1878002094694644 7 0.17779901488233316 8 0.18141055737213055 9 0.19030050811624724 10-11 0.18724458754795711 12-13 0.18321632861702924 14-15 0.18571662726381205 16-17 0.17085374086349203 18-19 0.17613214911781128 20-21 0.16613095453068005 22-23 0.1729373230691444 24-25 0.1718260792261298 26-27 0.17529871623555035 28-29 0.18543881630305842 30-31 0.1804382190094928 32-33 0.188911453312479 34-35 0.1834941395777829 36-37 0.1664087654914337 38-39 0.16057473531560715 40-41 0.15904677503146208 42-43 0.16988140250085426 44-45 0.17196498470650662 46-47 0.1725206066280139 48-49 0.18627224918531937 50-51 0.18446647794042065 52-53 0.2005795136641321 54-55 0.17224279566726025 56-57 0.15626866542392562 58-59 0.16099145175673762 60-61 0.1507124462088527 62-63 0.1548796106201574 64-65 0.15460179965940377 66-67 0.15446289417902695 68-69 0.15835224762957797 70-71 0.15751881474731702 72-73 0.15626866542392562 74-75 0.16599204905030324 76-77 0.1604358298352303 78-79 0.1641862778054045 80-81 0.15904677503146208 82-83 0.14557294343491028 84-85 0.1462674708367944 86-87 0.14557294343491028 88-89 0.15043463524809908 90-91 0.15349055581638918 92-93 0.15154587909111367 94-95 0.15390727225751966 96-97 0.15599085446317199 98-99 0.16543642712879592 100-101 0.15126806813036 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 359957.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 70.45985835581094 #Duplication Level Percentage of deduplicated Percentage of total 1 87.30765655487713 61.51685114234431 2 7.610146567749715 10.724196984612114 3 1.9727604528144977 4.1700126622576486 4 0.8487967219889028 2.392243872168589 5 0.5290444081595852 1.86381970314291 6 0.3375279185233251 1.426930159817111 7 0.23944153199663684 1.1809711500286983 8 0.16219730983077846 0.9142719581096186 9 0.12930321347473434 0.819961749574287 >10 0.810836307104006 10.281286291892226 >50 0.040801275670759356 1.8985188363782868 >100 0.009903222249925833 1.0759762002476616 >500 3.961288899970333E-4 0.1496039541719187 >1k 0.0011883866699911 1.585355335254661 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2436 0.6767475003958806 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2014 0.5595112749578423 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1230 0.34170748172698406 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 536 0.14890667496395404 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 424 0.11779184735954572 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.3890548037682278E-4 0.0 22-23 0.0 0.0 0.0 5.556219215072911E-4 0.0 24-25 0.0 0.0 0.0 5.556219215072911E-4 0.0 26-27 0.0 0.0 0.0 9.723383626377594E-4 0.0 28-29 0.0 0.0 0.0 0.0047227863328119755 0.0 30-31 0.0 0.0 0.0 0.009306667185247126 0.0 32-33 0.0 0.0 0.0 0.016113035723711443 0.0 34-35 0.0 0.0 0.0 0.020835822056523415 0.0 36-37 0.0 0.0 0.0 0.0298646782810169 0.0 38-39 0.0 0.0 0.0 0.042088360554177306 0.0 40-41 0.0 0.0 0.0 0.05820139627788874 0.0 42-43 0.0 0.0 0.0 0.08376000466722414 0.0 44-45 0.0 0.0 0.0 0.10529035412563167 0.0 46-47 0.0 0.0 0.0 0.15015682428734545 0.0 48-49 0.0 0.0 0.0 0.20169075750714668 0.0 50-51 0.0 0.0 0.0 0.22502687821045292 0.0 52-53 0.0 0.0 0.0 0.2679486716468912 0.0 54-55 0.0 0.0 0.0 0.3446244968148974 0.0 56-57 0.0 0.0 0.0 0.4644999263800954 0.0 58-59 0.0 0.0 0.0 0.6067391382859619 0.0 60-61 0.0 0.0 0.0 0.7739813366596566 0.0 62-63 0.0 0.0 0.0 0.9631706009328892 0.0 64-65 0.0 0.0 0.0 1.1623610597932532 0.0 66-67 0.0 0.0 0.0 1.3955833613459385 0.0 68-69 0.0 0.0 0.0 1.6724219837369465 0.0 70-71 0.0 0.0 0.0 1.8659173179018604 0.0 72-73 0.0 0.0 0.0 2.112335640090344 0.0 74-75 0.0 0.0 0.0 2.376811674727815 0.0 76-77 0.0 0.0 0.0 2.691571493261695 0.0 78-79 0.0 0.0 0.0 3.0160546954219534 0.0 80-81 0.0 0.0 0.0 3.5236153207188634 0.0 82-83 0.0 0.0 0.0 4.033815150142933 0.0 84-85 0.0 0.0 0.0 4.71556324783238 0.0 86-87 0.0 0.0 0.0 5.448150751339743 0.0 88-89 0.0 0.0 0.0 6.26324811019094 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1310 0.0 45.354294 1 GTATCAA 2305 0.0 37.530136 1 ATCAACG 2740 0.0 31.220587 3 TCAACGC 2770 0.0 30.88246 4 CAACGCA 2800 0.0 30.721308 5 TATCAAC 2810 0.0 30.447094 2 AACGCAG 2875 0.0 30.072609 6 AGTAGCG 65 0.00582002 29.25003 2 GTACATG 1985 0.0 28.25538 1 TACATGG 2040 0.0 27.726591 2 ACATGGG 1985 0.0 26.57551 3 ACGCAGA 3255 0.0 26.415884 7 AGGACCG 90 8.9084555E-4 26.402596 5 ACCGTCC 45 8.960819E-4 26.380531 62-63 CGCAGAG 3275 0.0 26.254564 8 GTATAGA 110 9.416558E-5 25.926163 1 GAGTACT 1940 0.0 25.826603 12-13 GTACTTT 2000 0.0 25.051804 14-15 TACCTGG 235 1.72804E-10 24.271301 2 GCAGAGT 3525 0.0 23.988243 9 >>END_MODULE