FastQCFastQC Report
Thu 26 May 2016
SRR937674_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937674_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359957
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29760.8267654192028493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26870.7464780515450457No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16930.4703339565559219No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6430.1786324477645941No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4850.1347383159655181No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.11168000622296553No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTTCG250.005019528356.9969989
CTACGCA601.4870966E-647.51073
TACGCAT652.580393E-643.856034
CTCTACG704.2911743E-640.729121
ACGCATT704.295276E-640.7234575
GGTATCA19550.039.1318971
CGCATTC756.9115486E-637.9986
TACCTGG2400.037.6126372
GGTAAGG1353.0559022E-1035.193113
CGTATCC352.0506949E-433.93385794-95
CATATCC1001.2936289E-633.257493
CCTACGT600.003943027531.67382
GTAAGGT1509.476935E-1031.67384
GTATCAA29550.030.8741871
TCTACGC953.4789005E-530.0067582
CCGTATC404.4933773E-429.68593692-93
CGCCGTA404.4933773E-429.68593690-91
GTACCTG3250.029.2414171
ACGTATA650.00582842729.2414151
CGCCCTA350.00838599227.14519926-27
Produced by FastQC (version 0.11.5)