##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937674_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 359957 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.45697958367249 33.0 31.0 34.0 31.0 34.0 2 32.67843103481805 34.0 31.0 34.0 31.0 34.0 3 32.72882316498915 34.0 31.0 34.0 31.0 34.0 4 36.28893173351261 37.0 35.0 37.0 35.0 37.0 5 36.22317665721183 37.0 35.0 37.0 35.0 37.0 6 36.280386268359834 37.0 37.0 37.0 35.0 37.0 7 36.23812288690038 37.0 37.0 37.0 35.0 37.0 8 36.22022630480863 37.0 37.0 37.0 35.0 37.0 9 37.94054567628911 39.0 38.0 39.0 35.0 39.0 10-11 37.884623718944205 39.0 38.0 39.0 35.0 39.0 12-13 37.8032681681423 39.0 38.0 39.0 35.0 39.0 14-15 39.05194509344172 40.0 38.0 41.0 36.0 41.0 16-17 39.017225668621535 40.0 38.0 41.0 36.0 41.0 18-19 39.03038279572282 40.0 38.0 41.0 36.0 41.0 20-21 38.95359445711571 40.0 38.0 41.0 35.5 41.0 22-23 38.81597107432276 40.0 38.0 41.0 35.0 41.0 24-25 38.87338904369133 40.0 38.0 41.0 35.0 41.0 26-27 38.73956333673189 40.0 38.0 41.0 35.0 41.0 28-29 38.60066896879349 40.0 38.0 41.0 35.0 41.0 30-31 38.617887692141004 40.0 38.0 41.0 35.0 41.0 32-33 38.501554352325414 40.0 38.0 41.0 35.0 41.0 34-35 38.36941773600736 40.0 38.0 41.0 35.0 41.0 36-37 38.202540859047055 40.0 38.0 41.0 34.0 41.0 38-39 38.04756262553583 40.0 38.0 41.0 34.0 41.0 40-41 37.85453540283979 40.0 37.0 41.0 33.5 41.0 42-43 37.61602358059435 40.0 37.0 41.0 33.0 41.0 44-45 37.2279230574763 40.0 37.0 41.0 32.5 41.0 46-47 36.75716960636965 40.0 37.0 41.0 31.5 41.0 48-49 36.32921293376709 40.0 36.0 41.0 30.0 41.0 50-51 36.03272752023159 40.0 36.0 41.0 29.5 41.0 52-53 35.78472984273121 39.0 36.0 41.0 28.5 41.0 54-55 35.56881933119789 39.0 35.0 41.0 28.0 41.0 56-57 35.31846026053112 39.0 35.0 40.0 27.0 41.0 58-59 35.06457854688199 38.5 35.0 40.0 26.5 41.0 60-61 34.70406048500237 38.0 34.5 40.0 26.0 41.0 62-63 34.367774484174504 38.0 34.0 40.0 25.5 41.0 64-65 34.005834030175826 37.5 34.0 40.0 24.5 41.0 66-67 33.59115672149729 37.0 33.5 39.5 22.5 41.0 68-69 33.138611000758424 36.0 33.0 39.0 21.5 40.5 70-71 32.69292721075017 36.0 32.5 39.0 20.0 40.0 72-73 32.205837086096395 35.0 32.0 38.0 18.5 40.0 74-75 31.714582575140916 35.0 31.5 37.5 16.5 39.5 76-77 30.484857913584122 34.0 30.0 36.0 13.5 38.5 78-79 31.034693032778915 35.0 31.0 36.0 12.0 39.0 80-81 30.81597524148718 35.0 31.0 36.0 7.5 38.5 82-83 30.454340379545336 34.0 31.0 36.0 7.0 37.0 84-85 30.06334089905183 34.0 31.0 35.0 2.0 37.0 86-87 29.648770547593184 34.0 30.0 35.0 2.0 36.0 88-89 29.293993449217545 34.0 30.0 35.0 2.0 36.0 90-91 28.798475929069305 34.0 29.0 35.0 2.0 36.0 92-93 28.341276874737815 34.0 29.0 35.0 2.0 35.0 94-95 27.883340232305525 34.0 28.5 35.0 2.0 35.0 96-97 27.36787171801076 33.0 27.0 35.0 2.0 35.0 98-99 26.543403795453344 33.0 25.5 35.0 2.0 35.0 100-101 23.959214572851756 30.5 13.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3071550383940149 1101 2 0.35285166211416197 1101 3 0.24426087652917516 1101 4 0.20003018061613886 1101 5 0.07794441120871198 1101 6 0.1549326740018273 1101 7 0.15099078948264832 1101 8 0.1476360300987949 1101 9 0.23240412752037543 1101 10-11 0.2860864330856643 1101 12-13 0.3251690662235518 1101 14-15 0.40213350601796094 1101 16-17 0.3210993012743586 1101 18-19 0.33093830467110763 1101 20-21 0.3582809672767908 1101 22-23 0.3089118289660675 1101 24-25 0.2753066528038417 1101 26-27 0.1875232674629288 1101 28-29 0.38130167059151887 1101 30-31 0.5064504146274587 1101 32-33 0.5503947925341919 1101 34-35 0.5034857564450235 1101 36-37 0.49569400134133446 1101 38-39 0.3359509621978489 1101 40-41 0.4064424329380216 1101 42-43 0.3390768078567916 1101 44-45 0.35124292792843903 1101 46-47 0.46055976417802214 1101 48-49 0.7073586181924796 1101 50-51 0.7678690360168261 1101 52-53 0.7429620402190906 1101 54-55 0.7332308266798506 1101 56-57 0.8025250974275338 1101 58-59 0.9360005193429188 1101 60-61 0.9783248129877862 1101 62-63 0.8305841362167712 1101 64-65 1.018961326617962 1101 66-67 0.7859132734253009 1101 68-69 0.9434081817113054 1101 70-71 0.9101536613142969 1101 72-73 0.8340049944115471 1101 74-75 0.8700928834213784 1101 76-77 0.7482017467861226 1101 78-79 0.9726465388803867 1101 80-81 0.5627075846547775 1101 82-83 0.6847138001593827 1101 84-85 0.656003740620875 1101 86-87 0.34357100728108136 1101 88-89 0.4367056812245522 1101 90-91 0.6262734979422255 1101 92-93 0.5852737978882097 1101 94-95 0.4344388833258357 1101 96-97 0.7898896473013473 1101 98-99 0.5993515334230963 1101 100-101 0.609023475392032 1102 1 0.21325223577028396 1102 2 0.17557825722447973 1102 3 0.24387327962995187 1102 4 0.13237216034183774 1102 5 0.0906754785909456 1102 6 0.11359397394220139 1102 7 0.1569985414206272 1102 8 0.16835010284775365 1102 9 0.060914860972971496 1102 10-11 0.2979118654052826 1102 12-13 0.1326414872134123 1102 14-15 0.28170267715690045 1102 16-17 0.24590550282474766 1102 18-19 0.12211302142721792 1102 20-21 0.187529625595225 1102 22-23 0.20440601501258726 1102 24-25 0.2192951739725899 1102 26-27 0.24413104921606532 1102 28-29 -0.014840994882540315 1102 30-31 0.24263854819931652 1102 32-33 0.030869598735748127 1102 34-35 0.17819133187735048 1102 36-37 0.15088929054824973 1102 38-39 0.2150020951733964 1102 40-41 0.13291828266968508 1102 42-43 0.2574354840643025 1102 44-45 0.23171773412999386 1102 46-47 0.24957288284230827 1102 48-49 0.15356836774525107 1102 50-51 0.12930687143722253 1102 52-53 0.2060956120735966 1102 54-55 0.38669683741330374 1102 56-57 0.3295115315360633 1102 58-59 0.28733102023738155 1102 60-61 0.2295025112585094 1102 62-63 0.2734285011541573 1102 64-65 -0.018881547562124013 1102 66-67 -0.02061997046259023 1102 68-69 0.04165281498499951 1102 70-71 -0.008700691697036689 1102 72-73 0.14717583519867006 1102 74-75 0.016187099283030193 1102 76-77 0.03588063766268945 1102 78-79 0.09369603202290122 1102 80-81 0.09675648149437066 1102 82-83 0.04234647755950505 1102 84-85 0.1568422916047716 1102 86-87 0.36981206273724965 1102 88-89 0.1529661165256826 1102 90-91 0.32559893026184383 1102 92-93 0.3196171192954864 1102 94-95 0.322490463528581 1102 96-97 0.5832781386549541 1102 98-99 0.1810711219740817 1102 100-101 0.5591315552966236 1103 1 0.3237064644960057 1103 2 0.3472913048684134 1103 3 0.2764706143568887 1103 4 0.19593914057004014 1103 5 0.19312735560514227 1103 6 0.1163703000461993 1103 7 0.16016681944519462 1103 8 0.2304363758204886 1103 9 0.26252513011328915 1103 10-11 0.09897176872380697 1103 12-13 0.17281383718293597 1103 14-15 0.2085437624282136 1103 16-17 0.40358993797848086 1103 18-19 0.3438380453798331 1103 20-21 0.2868894374582993 1103 22-23 0.25610284020202556 1103 24-25 0.05989322727805302 1103 26-27 0.33537691194578656 1103 28-29 0.22399109726971034 1103 30-31 0.42184223254736963 1103 32-33 0.45854085994992033 1103 34-35 0.5159749526420896 1103 36-37 0.39099794833067847 1103 38-39 0.41225462108577204 1103 40-41 0.5042600647444218 1103 42-43 0.9084948430051654 1103 44-45 0.9782092200632704 1103 46-47 0.6422869321411468 1103 48-49 0.6595265813155038 1103 50-51 0.6834697274602135 1103 52-53 0.6527574882167926 1103 54-55 0.610974992626268 1103 56-57 0.54915431091068 1103 58-59 0.4438368777410773 1103 60-61 0.5455028596602176 1103 62-63 0.7074063776457535 1103 64-65 0.511881234425509 1103 66-67 0.40887495593754863 1103 68-69 0.8071866317257061 1103 70-71 0.8827912297383804 1103 72-73 0.6234028624611021 1103 74-75 0.5590513968180915 1103 76-77 0.4871098424357889 1103 78-79 0.5988349572036391 1103 80-81 0.727515997215999 1103 82-83 0.8726639586151599 1103 84-85 0.8810469558326268 1103 86-87 0.7682829058693805 1103 88-89 0.49709173711622157 1103 90-91 0.8450577099848608 1103 92-93 0.6463008794209095 1103 94-95 0.3821052618910912 1103 96-97 0.5469409293526297 1103 98-99 0.873652887499734 1103 100-101 1.156387347475004 1104 1 0.0502982247956254 1104 2 -0.018813564045444764 1104 3 -0.15892264407007417 1104 4 -0.06342415531010914 1104 5 -0.11679773802782023 1104 6 0.11356131128215452 1104 7 -0.07297924797053668 1104 8 -0.21525650807463848 1104 9 -0.17848610584177038 1104 10-11 -0.0850169953211406 1104 12-13 0.06654042894322743 1104 14-15 -0.066268971654182 1104 16-17 -0.2518407736320043 1104 18-19 -0.047079557616420686 1104 20-21 -0.21591955130573837 1104 22-23 0.04121519975258536 1104 24-25 -0.028589918789357682 1104 26-27 -0.1313833876796764 1104 28-29 -0.02788156190631952 1104 30-31 -0.07534877868859269 1104 32-33 -0.1697362936081248 1104 34-35 -0.053313436871015085 1104 36-37 0.2889380232370371 1104 38-39 -0.05871916168576519 1104 40-41 -0.08422308132299605 1104 42-43 0.1215098242785757 1104 44-45 0.12661745976364358 1104 46-47 0.012605284201072209 1104 48-49 -0.28944345613768263 1104 50-51 -0.14377746355101806 1104 52-53 -0.3879728488618568 1104 54-55 -0.1249800635535081 1104 56-57 -0.044010483096812436 1104 58-59 0.1385934320481894 1104 60-61 0.03613956378006833 1104 62-63 0.1376498233386343 1104 64-65 0.11534565390116569 1104 66-67 0.11439930050683245 1104 68-69 -0.09215793756268198 1104 70-71 -0.2066282459170523 1104 72-73 -0.13596043341904718 1104 74-75 -0.09310216123434145 1104 76-77 0.013327070900210458 1104 78-79 0.2992094890388408 1104 80-81 0.030418618938842457 1104 82-83 0.3010347451320179 1104 84-85 0.21110313560791027 1104 86-87 0.07680350512032064 1104 88-89 -0.2388633190635545 1104 90-91 -0.08893105406007962 1104 92-93 -0.24555305316335918 1104 94-95 0.09465207837049405 1104 96-97 0.0076150866559991925 1104 98-99 0.6273982058517937 1104 100-101 0.6868180590854926 1105 1 0.3099534944182807 1105 2 0.3606818247830361 1105 3 0.3130780808302518 1105 4 -0.00644900328239828 1105 5 0.1928051776823807 1105 6 0.026603476317795582 1105 7 0.11030978589841567 1105 8 0.2956371329386087 1105 9 0.2171987236052928 1105 10-11 0.2103144452169019 1105 12-13 0.26684951838787896 1105 14-15 0.3985079201343069 1105 16-17 0.29753299303063585 1105 18-19 0.2366937499426811 1105 20-21 0.10653020907442823 1105 22-23 0.1531770619409798 1105 24-25 0.1886234435399885 1105 26-27 0.038681249266076634 1105 28-29 0.38793938771311076 1105 30-31 0.4600001802739939 1105 32-33 0.4861474807062365 1105 34-35 0.27504627477973287 1105 36-37 0.13925617393576317 1105 38-39 0.384255345986098 1105 40-41 0.5544090720336925 1105 42-43 0.37730399285817384 1105 44-45 0.568268017534173 1105 46-47 1.0237455926864314 1105 48-49 0.9255347968525314 1105 50-51 0.5011472273595388 1105 52-53 0.9095735648951333 1105 54-55 0.8758555854336478 1105 56-57 1.0532419433056717 1105 58-59 1.141583645893796 1105 60-61 1.1167243167098704 1105 62-63 1.150793675781145 1105 64-65 1.0402530277483706 1105 66-67 1.203365713458382 1105 68-69 0.9198280879699183 1105 70-71 1.1062316872310305 1105 72-73 1.1851905472100093 1105 74-75 0.9276128723929773 1105 76-77 1.2516481212002368 1105 78-79 1.6072619234957664 1105 80-81 1.2600056271141078 1105 82-83 1.379399490812819 1105 84-85 1.5194928500777287 1105 86-87 1.5752983301374321 1105 88-89 1.38733981411675 1105 90-91 1.291092907922927 1105 92-93 1.9872658022995715 1105 94-95 1.9149875461325614 1105 96-97 1.7772276677038832 1105 98-99 2.0585326748623025 1105 100-101 2.7864566157283974 1106 1 0.09648237772135104 1106 2 0.09963820890071418 1106 3 -0.21547938321108262 1106 4 -0.15731247672651705 1106 5 -0.16068362741932418 1106 6 -0.12979926073010262 1106 7 0.007484295976148303 1106 8 -0.044771562308795865 1106 9 0.10208444720069565 1106 10-11 -0.21572009372086143 1106 12-13 -0.44713363107914716 1106 14-15 -0.06418351029905978 1106 16-17 -0.045459140284087596 1106 18-19 -0.11425816552536361 1106 20-21 -0.022804613808780516 1106 22-23 -0.14585007579583475 1106 24-25 0.07181014930733198 1106 26-27 0.1073867373264008 1106 28-29 0.060680624970480324 1106 30-31 -0.006461673284626102 1106 32-33 0.1680354851748831 1106 34-35 0.09890700186627299 1106 36-37 -0.12974722409988715 1106 38-39 0.03968056592744773 1106 40-41 0.023850025345616643 1106 42-43 0.23146775024773092 1106 44-45 -0.07105144436592781 1106 46-47 -0.003950725332465765 1106 48-49 0.12641456224842784 1106 50-51 0.07353004167782728 1106 52-53 -0.0591691619121022 1106 54-55 -0.31631962287059423 1106 56-57 -0.5284272835248487 1106 58-59 -0.09302045967805128 1106 60-61 -0.022083112420133943 1106 62-63 0.051063656696285875 1106 64-65 0.14068127333790414 1106 66-67 0.09507078258280899 1106 68-69 0.20856802187114454 1106 70-71 0.2333554595160976 1106 72-73 -0.22601831561176056 1106 74-75 -0.11404797571948322 1106 76-77 -0.21600071741634252 1106 78-79 -0.45767314143929383 1106 80-81 -0.12830140635421472 1106 82-83 0.053886294331878304 1106 84-85 0.18892914854628273 1106 86-87 0.16234390105397623 1106 88-89 0.5094829040017714 1106 90-91 0.44535441702314316 1106 92-93 0.2983024359168489 1106 94-95 0.2932633921503438 1106 96-97 -0.30262617021447014 1106 98-99 0.2145862986578635 1106 100-101 1.0963528127213706 1107 1 0.3092220190324255 1107 2 0.5047093282575474 1107 3 0.28915883571284695 1107 4 0.1358229592660578 1107 5 0.2468612086969415 1107 6 0.3303120565240718 1107 7 0.22207922485679177 1107 8 0.1902315298371562 1107 9 0.11940046451670838 1107 10-11 0.2697468203174793 1107 12-13 0.4356740374428085 1107 14-15 0.3037818576663085 1107 16-17 0.11378637610429365 1107 18-19 0.7617467819081511 1107 20-21 0.584183636036606 1107 22-23 0.4902043459728773 1107 24-25 0.5316122519665853 1107 26-27 0.6213013940982037 1107 28-29 0.5919112990582889 1107 30-31 0.41530703419836357 1107 32-33 0.49580295579950473 1107 34-35 0.3165940766962052 1107 36-37 0.3832397888871242 1107 38-39 0.3161184137938662 1107 40-41 0.07456121891394929 1107 42-43 0.24878376756375076 1107 44-45 0.6413789823502114 1107 46-47 -0.16566995848027233 1107 48-49 -0.21403070676826985 1107 50-51 -0.11632771960850619 1107 52-53 -0.2010986609844636 1107 54-55 0.15677870737059152 1107 56-57 -0.23591027672212306 1107 58-59 -0.18176504842264052 1107 60-61 -0.3028499646155609 1107 62-63 -0.3626654713185502 1107 64-65 -0.0027942987090980864 1107 66-67 0.27534289142634805 1107 68-69 0.13528416287299905 1107 70-71 0.23892132222481877 1107 72-73 0.7040991859343073 1107 74-75 0.38193223454644 1107 76-77 0.05641734071599913 1107 78-79 0.48481294390393614 1107 80-81 0.3778828855443628 1107 82-83 0.5934657624827757 1107 84-85 0.3877541330855543 1107 86-87 0.6555411799655353 1107 88-89 0.6022472824804375 1107 90-91 0.6107842253100948 1107 92-93 1.042272751315739 1107 94-95 1.2784210915937564 1107 96-97 1.0690497728900468 1107 98-99 1.1759344742917541 1107 100-101 1.842487630284758 1108 1 0.21497141556523047 1108 2 0.20297573159307092 1108 3 -0.12240579013749198 1108 4 0.08853307557064483 1108 5 0.24477650384725536 1108 6 0.11800132578015621 1108 7 0.038625198084794476 1108 8 0.07699914010706976 1108 9 0.2530410175121034 1108 10-11 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-0.6212377287126785 2316 66-67 -0.9569342299485157 2316 68-69 -0.9028468790174458 2316 70-71 -0.5472181335575002 2316 72-73 -0.41371767765322787 2316 74-75 -0.14163113817106776 2316 76-77 -0.17816464997501313 2316 78-79 -0.6438345677323021 2316 80-81 -0.21995098484589803 2316 82-83 0.0654492927369823 2316 84-85 0.23368592685569567 2316 86-87 0.1870932744875553 2316 88-89 -0.18512292876195602 2316 90-91 0.5355515006412794 2316 92-93 -0.11815062856253178 2316 94-95 -0.41259215581046504 2316 96-97 -0.48266433380526763 2316 98-99 -0.2237253896538256 2316 100-101 -0.07279163931782051 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 1.0 4 2.0 5 6.0 6 11.0 7 21.0 8 74.0 9 130.0 10 276.0 11 425.0 12 728.0 13 1120.0 14 1444.0 15 2219.0 16 3227.0 17 5354.0 18 4817.0 19 2618.0 20 1905.0 21 1752.0 22 1824.0 23 1970.0 24 2107.0 25 2486.0 26 2894.0 27 3430.0 28 4213.0 29 5184.0 30 6563.0 31 8284.0 32 10693.0 33 14645.0 34 21188.0 35 31783.0 36 51779.0 37 83703.0 38 73453.0 39 7627.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.20054906886146 20.600641321321987 15.608727305061104 20.590082304755448 2 15.179313084618439 21.257261283986697 41.381887280980784 22.181538350414076 3 19.1692341029623 26.291473703803508 31.141775267601407 23.397516925632782 4 12.670124487091513 18.07049175318163 37.988982017296514 31.270401742430348 5 12.555226435331582 38.801051455754944 34.34736941553065 14.296352693382829 6 30.295007459224294 38.019819033940166 17.12398980989396 14.56118369694158 7 27.136296835455347 34.84193945387921 20.805818472762024 17.215945237903416 8 26.295085246293308 35.71954427889998 19.372313915273214 18.6130565595335 9 26.838205674566684 17.327347433165627 21.07918445814361 34.75526243412407 10-11 24.482507632856144 28.309770333678745 26.781948955014073 20.425773078451037 12-13 25.804117023596696 25.668540566670693 28.14364436739553 20.38369804233708 14-15 22.552277077539816 26.669157704948095 27.294649083084927 23.48391613442717 16-17 20.67177468419839 30.21735929569365 27.99306583841959 21.117800181688366 18-19 20.410076759168454 27.777067816433632 31.953677800403934 19.859177623993975 20-21 21.275874618357193 26.805979603119262 32.397202999247135 19.520942779276414 22-23 21.093894333280087 26.98578284634778 31.958105583375584 19.962217236996548 24-25 20.85790802790333 27.06795533910995 32.42581752820476 19.64831910478196 26-27 20.631137077428058 27.239629563263033 32.409602907042164 19.719630452266752 28-29 20.30670926517572 27.282678149743024 32.57063481039033 19.83997777469093 30-31 20.362987801320713 27.106293251693952 32.4519317585156 20.078787188469736 32-33 20.419661237314457 27.284647888497794 32.18148278822193 20.114208085965824 34-35 20.763035584805962 27.18533047002836 32.29677433693469 19.754859608230984 36-37 20.911247732368032 26.906408265431704 32.11119661515126 20.07114738704901 38-39 20.532452487380436 27.409246104395802 32.371644390857796 19.686657017365963 40-41 20.599854425843304 27.437255871583595 32.160081565977876 19.80280813659523 42-43 20.738386131433508 27.4978952077423 31.63495816812219 20.128760492701996 44-45 20.816236383790283 27.664415471848024 31.224979650347123 20.294368494014563 46-47 20.99667460279978 28.153362762774442 30.724503204549432 20.125459429876347 48-49 21.132385257127932 28.388113024611272 30.264726064502153 20.214775653758643 50-51 21.242259492106 28.523129151537546 29.725356084198946 20.50925527215751 52-53 21.214200585069882 28.815108471289623 29.62589976024914 20.34479118339135 54-55 20.96069808338218 28.783299116283334 29.54338990490531 20.712612895429174 56-57 21.168639587506284 29.00999286025831 29.565198065324473 20.256169486910935 58-59 21.2057273507669 28.46909491967096 29.522970799289915 20.802206930272227 60-61 21.07821211978097 28.610083982253435 30.041921673977722 20.269782223987864 62-63 21.091269234936394 28.53701969957523 30.170825959767416 20.20088510572096 64-65 20.85485210733504 28.42020019057832 29.963301172084446 20.761646530002196 66-67 20.77192553555008 28.73579344199446 29.647291204227173 20.84498981822829 68-69 20.89180096511528 28.600082787666302 29.69840842100584 20.809707826212577 70-71 20.986534502732273 28.943318229677434 29.50046811146887 20.569679156121428 72-73 20.971393805371196 28.577719005325637 29.55255766661018 20.898329522692986 74-75 20.849990415521855 28.903452356809282 29.083751670338405 21.162805557330458 76-77 20.966115397116877 28.75496239828646 29.408790494420167 20.870131710176494 78-79 21.040152018157723 28.53993671466314 29.03527365768689 21.384637609492245 80-81 20.947224251785627 29.02652261242315 28.86636459354867 21.15988854224255 82-83 21.055139285942495 28.80817240507525 28.96639190093987 21.170296408042386 84-85 20.832523044611257 28.661717886174344 29.024819838090423 21.480939231123976 86-87 20.77567598352025 28.771631055931678 28.982628480624072 21.47006447992399 88-89 20.623574482507635 28.50798845417647 29.099725800581737 21.76871126273416 90-91 20.50638362901321 28.642419527375278 29.23396451841458 21.617232325196927 92-93 20.573584444376532 28.63893851039181 29.019689009770882 21.767788035460775 94-95 20.181855054909338 28.782326777920698 28.876782504576937 22.159035662593034 96-97 20.47897387743536 29.03444022480463 28.656061696258163 21.83052420150185 98-99 20.303651176196862 29.228439863593113 28.394024211527906 22.07388474868212 100-101 20.08294661961264 29.382554811459695 28.24422708200183 22.290271486925832 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 196.0 1 143.0 2 93.5 3 105.5 4 112.0 5 127.0 6 190.0 7 385.5 8 671.0 9 860.5 10 843.0 11 770.0 12 816.0 13 909.0 14 960.5 15 975.0 16 1028.5 17 1084.5 18 1088.0 19 1142.5 20 1228.5 21 1311.5 22 1485.0 23 1703.5 24 1952.5 25 2325.5 26 2914.0 27 3498.0 28 3892.5 29 4582.0 30 5512.5 31 6222.0 32 6952.0 33 7887.5 34 9028.5 35 9850.0 36 11000.5 37 12043.5 38 12474.5 39 12721.5 40 12569.0 41 12664.5 42 12804.0 43 13086.5 44 13483.5 45 13612.5 46 13167.0 47 13019.0 48 13734.5 49 13183.0 50 11974.5 51 11205.5 52 10316.0 53 9571.0 54 8934.0 55 8156.5 56 7051.0 57 6018.0 58 5206.0 59 4494.0 60 3759.5 61 2898.0 62 2186.5 63 1695.5 64 1218.0 65 839.0 66 591.0 67 477.0 68 341.0 69 197.5 70 139.5 71 91.5 72 70.0 73 58.0 74 47.0 75 34.5 76 23.5 77 14.0 78 11.5 79 10.0 80 7.0 81 3.5 82 2.5 83 1.5 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.020835822056523418 2 0.0 3 0.0 4 0.0 5 0.020835822056523418 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0034726370094205696 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012501493233914051 24-25 0.0 26-27 9.723383626377596E-4 28-29 0.0019446767252755191 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 8.334328822609368E-4 42-43 0.017779901488233316 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.0038893534505510382 84-85 8.334328822609368E-4 86-87 0.0 88-89 0.0 90-91 0.01444616995918957 92-93 0.0027781096075364556 94-95 0.0 96-97 0.0 98-99 0.0012501493233914051 100-101 0.024169553585567164 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 359957.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.4231069903762 #Duplication Level Percentage of deduplicated Percentage of total 1 85.41694793441185 56.73659071438833 2 8.756132744980693 11.632190842835781 3 2.3149626857585077 4.61301042464598 4 1.0124300686101615 2.689950030702667 5 0.5616222107991611 1.8652346098042147 6 0.3709261068677089 1.4782838689198525 7 0.26818850837702407 1.2469739789861531 8 0.20323169717016012 1.0799424611975428 9 0.13614053032479714 0.8138589310341501 >10 0.8773246616605569 10.584630490299167 >50 0.056703309480873626 2.604163326802068 >100 0.02369690545459411 2.406848415254727 >500 4.231590259748948E-4 0.18073146455784067 >1k 0.0012694770779246845 2.0675904405715024 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2976 0.8267654192028493 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2687 0.7464780515450457 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1693 0.4703339565559219 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 643 0.1786324477645941 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 485 0.1347383159655181 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 402 0.11168000622296553 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.3890548037682278E-4 0.0 22-23 0.0 0.0 0.0 5.556219215072911E-4 0.0 24-25 0.0 0.0 0.0 5.556219215072911E-4 0.0 26-27 0.0 0.0 0.0 9.723383626377594E-4 0.0 28-29 0.0 0.0 0.0 0.004444975372058329 0.0 30-31 0.0 0.0 0.0 0.009028856224493481 0.0 32-33 0.0 0.0 0.0 0.015835224762957796 0.0 34-35 0.0 0.0 0.0 0.02028020013501613 0.0 36-37 0.0 0.0 0.0 0.029864678281016903 0.0 38-39 0.0 0.0 0.0 0.041949455073800476 0.0 40-41 0.0 0.0 0.0 0.05778467983675828 0.0 42-43 0.0 0.0 0.0 0.08334328822609367 0.0 44-45 0.0 0.0 0.0 0.10570707056676214 0.0 46-47 0.0 0.0 0.0 0.15126806813036 0.0 48-49 0.0 0.0 0.0 0.20349652875204538 0.0 50-51 0.0 0.0 0.0 0.22641593301422114 0.0 52-53 0.0 0.0 0.0 0.2705878757740508 0.0 54-55 0.0 0.0 0.0 0.34781932286356426 0.0 56-57 0.0 0.0 0.0 0.46838927983064643 0.0 58-59 0.0 0.0 0.0 0.6128509794225422 0.0 60-61 0.0 0.0 0.0 0.783843625766411 0.0 62-63 0.0 0.0 0.0 0.9742830393630351 0.0 64-65 0.0 0.0 0.0 1.175001458507544 0.0 66-67 0.0 0.0 0.0 1.4091960984228673 0.0 68-69 0.0 0.0 0.0 1.6895073578232955 0.0 70-71 0.0 0.0 0.0 1.8831415974685866 0.0 72-73 0.0 0.0 0.0 2.1327547457057374 0.0 74-75 0.0 0.0 0.0 2.4008423228330047 0.0 76-77 0.0 0.0 0.0 2.7212972660623356 0.0 78-79 0.0 0.0 0.0 3.050503254555405 0.0 80-81 0.0 0.0 0.0 3.5652869648319103 0.0 82-83 0.0 0.0 0.0 4.081043013471053 0.0 84-85 0.0 0.0 0.0 4.7705698180616025 0.0 86-87 0.0 0.0 0.0 5.513714138077604 0.0 88-89 0.0 0.0 0.0 6.341590801123468 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CAGTTCG 25 0.0050195283 56.996998 9 CTACGCA 60 1.4870966E-6 47.5107 3 TACGCAT 65 2.580393E-6 43.85603 4 CTCTACG 70 4.2911743E-6 40.72912 1 ACGCATT 70 4.295276E-6 40.723457 5 GGTATCA 1955 0.0 39.131897 1 CGCATTC 75 6.9115486E-6 37.998 6 TACCTGG 240 0.0 37.612637 2 GGTAAGG 135 3.0559022E-10 35.19311 3 CGTATCC 35 2.0506949E-4 33.933857 94-95 CATATCC 100 1.2936289E-6 33.25749 3 CCTACGT 60 0.0039430275 31.6738 2 GTAAGGT 150 9.476935E-10 31.6738 4 GTATCAA 2955 0.0 30.874187 1 TCTACGC 95 3.4789005E-5 30.006758 2 CCGTATC 40 4.4933773E-4 29.685936 92-93 CGCCGTA 40 4.4933773E-4 29.685936 90-91 GTACCTG 325 0.0 29.241417 1 ACGTATA 65 0.005828427 29.241415 1 CGCCCTA 35 0.008385992 27.145199 26-27 >>END_MODULE