FastQCFastQC Report
Thu 26 May 2016
SRR937673_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937673_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302335
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21750.719400664825442No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17530.5798203979029884No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10700.3539120512014818No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4750.1571104900193494No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN3920.1296574991317578No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3050.10088147253874014No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9350.049.299861
TGAACCG459.6085534E-442.2268685
GTATCAA16100.036.304861
AACGTTG550.002577326334.543528
CCTACGA452.4106279E-531.66752284-85
GTGCTAA804.4742643E-429.7006231
GGTACGA404.4926032E-429.6858418-19
GTACATG14250.029.6797811
TAGGACA1802.0918378E-1029.0357884
CCAGTAC1004.9318645E-528.5078683
ATCAACG20300.028.320623
TATCAAC20550.028.2072942
TCAACGC20850.027.5735574
CATGGGG5550.027.3949454
TACATGG15050.027.1503492
GAACGTT700.00838420727.141347
CAACGCA21450.027.0192895
AACGCAG21700.026.7035776
AGACGAT458.945512E-426.38741376-77
ACGCTCA458.949846E-426.38522340-41
Produced by FastQC (version 0.11.5)