##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937673_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 302335 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.01241999768469 33.0 31.0 34.0 31.0 34.0 2 32.15317776638497 34.0 31.0 34.0 31.0 34.0 3 32.16002778374981 34.0 31.0 34.0 30.0 34.0 4 35.72451750541618 37.0 35.0 37.0 35.0 37.0 5 35.71087039211471 37.0 35.0 37.0 35.0 37.0 6 35.770734450195974 37.0 35.0 37.0 35.0 37.0 7 35.730199943770984 37.0 35.0 37.0 35.0 37.0 8 35.716139381811566 37.0 35.0 37.0 35.0 37.0 9 37.389600939355354 39.0 37.0 39.0 35.0 39.0 10-11 37.29330378553591 39.0 37.0 39.0 35.0 39.0 12-13 37.18094167066334 39.0 37.0 39.0 34.0 39.0 14-15 38.388750888914615 40.0 38.0 41.0 34.0 41.0 16-17 38.337529561579046 40.0 38.0 41.0 34.0 41.0 18-19 38.30375742140341 40.0 38.0 41.0 34.0 41.0 20-21 38.25738171233896 40.0 38.0 41.0 34.0 41.0 22-23 38.07356574660558 40.0 38.0 41.0 33.5 41.0 24-25 37.92818562190947 40.0 38.0 41.0 33.0 41.0 26-27 37.860725685084425 40.0 38.0 41.0 33.0 41.0 28-29 37.88494550746688 40.0 38.0 41.0 33.0 41.0 30-31 37.68748738981593 40.0 37.5 41.0 33.0 41.0 32-33 37.43911389683629 40.0 37.0 41.0 33.0 41.0 34-35 37.467418591959245 40.0 37.0 41.0 33.0 41.0 36-37 37.39636330560471 40.0 37.0 41.0 33.0 41.0 38-39 37.25601898556237 40.0 37.0 41.0 32.5 41.0 40-41 37.023432616137725 40.0 36.5 41.0 31.5 41.0 42-43 36.77428514727041 40.0 36.0 41.0 31.0 41.0 44-45 36.50786875485802 40.0 36.0 41.0 30.5 41.0 46-47 36.079438371343045 39.5 36.0 41.0 29.5 41.0 48-49 35.665020589743165 39.0 35.0 41.0 27.5 41.0 50-51 34.734938065391034 38.5 34.0 40.0 25.0 40.5 52-53 34.82457704202292 38.5 35.0 40.0 26.0 40.5 54-55 35.16513304777813 39.0 35.0 40.0 26.0 41.0 56-57 34.96771296740371 39.0 35.0 40.0 25.0 41.0 58-59 34.75780839135396 39.0 35.0 40.0 24.5 41.0 60-61 34.450007442075844 38.0 34.0 40.0 23.0 41.0 62-63 34.11978269138538 38.0 34.0 40.0 22.0 41.0 64-65 33.75222352688243 38.0 34.0 40.0 20.5 41.0 66-67 33.356525046719696 37.0 33.5 40.0 18.5 41.0 68-69 32.897301007160934 36.5 33.0 39.0 17.0 41.0 70-71 32.46463856318322 36.0 32.0 39.0 12.5 40.5 72-73 32.026702168124764 35.5 32.0 39.0 8.0 40.0 74-75 31.564134155820533 35.0 31.5 38.0 7.0 40.0 76-77 31.07305968544826 35.0 31.0 37.0 2.0 39.0 78-79 30.54432500372104 35.0 30.5 36.5 2.0 39.0 80-81 30.015008186283424 34.5 30.0 36.0 2.0 38.5 82-83 29.504327980551373 34.0 29.0 36.0 2.0 37.0 84-85 29.042266029404466 34.0 29.0 35.0 2.0 37.0 86-87 28.58571452197066 34.0 29.0 35.0 2.0 36.5 88-89 28.1379694709511 34.0 28.0 35.0 2.0 36.0 90-91 27.675894289447136 34.0 27.0 35.0 2.0 36.0 92-93 27.163717399573322 33.0 26.0 35.0 2.0 35.0 94-95 26.57786395885359 33.0 25.0 35.0 2.0 35.0 96-97 25.88724262821043 32.5 24.0 35.0 2.0 35.0 98-99 24.992371045363587 32.0 18.5 34.0 2.0 35.0 100-101 22.64247440752807 29.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.02105750956333452 1101 2 -0.09267797466419836 1101 3 -0.160271945670047 1101 4 -0.1602494651632398 1101 5 0.06065000100637974 1101 6 -0.03951531996282398 1101 7 -0.07459271920040322 1101 8 -0.18326991884055843 1101 9 0.059844533063959204 1101 10-11 0.07965899107272634 1101 12-13 0.03133254795901763 1101 14-15 0.11037269880014833 1101 16-17 0.1865119401882538 1101 18-19 0.036211932824478765 1101 20-21 0.15064314776878973 1101 22-23 0.14776926596173467 1101 24-25 0.16363266163399004 1101 26-27 0.11617382233914952 1101 28-29 0.0017686041277968911 1101 30-31 0.03792047078152905 1101 32-33 0.27718284958434225 1101 34-35 0.6568149560598755 1101 36-37 0.5704047139155932 1101 38-39 0.6599237574102403 1101 40-41 0.5183696242118145 1101 42-43 0.41692926259381125 1101 44-45 0.6846946164466701 1101 46-47 0.7512747821659573 1101 48-49 0.6106880974942186 1101 50-51 0.8799537539401854 1101 52-53 0.9929739813864629 1101 54-55 1.0226974160363014 1101 56-57 1.1420435306858678 1101 58-59 1.313600468319585 1101 60-61 1.231574090065358 1101 62-63 1.2000304569859637 1101 64-65 1.127069083678144 1101 66-67 1.26139165068237 1101 68-69 1.1404909709321842 1101 70-71 0.9407444483172895 1101 72-73 1.0316977536888636 1101 74-75 1.2296850109304565 1101 76-77 1.2323417433849748 1101 78-79 1.3042128798692687 1101 80-81 1.099253409302996 1101 82-83 1.3715975308833563 1101 84-85 1.3249172032540208 1101 86-87 1.3574281243318467 1101 88-89 1.3497202918579347 1101 90-91 1.3729725012712173 1101 92-93 1.300217303424418 1101 94-95 1.333974272436663 1101 96-97 1.316083656155456 1101 98-99 1.50031623467083 1101 100-101 1.869290261146272 1102 1 0.05826458751646513 1102 2 -0.05549480871489365 1102 3 0.17688730827543253 1102 4 0.1733947242198326 1102 5 0.15464808804607344 1102 6 0.06952341748337432 1102 7 -0.07970988801915269 1102 8 0.1772991868504974 1102 9 0.36426825377175476 1102 10-11 0.021241965726005674 1102 12-13 0.05290117541000683 1102 14-15 0.4480460608278847 1102 16-17 0.3213756417664442 1102 18-19 0.20715884817598607 1102 20-21 0.1615948455210514 1102 22-23 0.049227898195113085 1102 24-25 0.15868287684202897 1102 26-27 -0.09660714370580337 1102 28-29 0.11407133009623749 1102 30-31 0.030878385654794727 1102 32-33 0.18697481515106062 1102 34-35 0.5629603410431372 1102 36-37 0.48103359961621805 1102 38-39 0.42802514430646 1102 40-41 0.2895437035996622 1102 42-43 0.37920807655842026 1102 44-45 0.5397759172596679 1102 46-47 0.27248472955954384 1102 48-49 0.19337580672425503 1102 50-51 0.2893511714437693 1102 52-53 0.21826092543237507 1102 54-55 0.4331350057063119 1102 56-57 0.1555538128475078 1102 58-59 0.04975541810437534 1102 60-61 0.25277208145703156 1102 62-63 0.09239057176358045 1102 64-65 0.1571145164854144 1102 66-67 0.3123242188315771 1102 68-69 0.1931093353511244 1102 70-71 0.07882431441006332 1102 72-73 0.4845791501498873 1102 74-75 0.5306773590892284 1102 76-77 0.5293407869048217 1102 78-79 0.3968240706870638 1102 80-81 0.7953318406755479 1102 82-83 0.6895554457566213 1102 84-85 0.650060675276972 1102 86-87 0.9634539492959746 1102 88-89 1.04994745589428 1102 90-91 0.9720997131315734 1102 92-93 1.5018791876903208 1102 94-95 1.2725323786059626 1102 96-97 0.9335035509426426 1102 98-99 1.083936512050073 1102 100-101 1.6122840440252801 1103 1 0.4108273305351986 1103 2 0.4885666971175553 1103 3 0.40665047547232547 1103 4 0.5259397931487797 1103 5 0.5657650905204434 1103 6 0.4177736825947207 1103 7 0.583634049768051 1103 8 0.5964402260869761 1103 9 0.547142060770696 1103 10-11 0.5367774906464717 1103 12-13 0.5311620449752255 1103 14-15 0.6156582912980184 1103 16-17 0.456034531833609 1103 18-19 0.5629382755354797 1103 20-21 0.494291911622156 1103 22-23 0.5337453955439955 1103 24-25 0.46124561986076174 1103 26-27 0.35151056573216266 1103 28-29 0.3502767030195315 1103 30-31 0.65552490385911 1103 32-33 0.6559126023550235 1103 34-35 0.6499522109618354 1103 36-37 0.8467900506589885 1103 38-39 0.9917225638893541 1103 40-41 1.193476188576696 1103 42-43 1.2627093137447076 1103 44-45 1.1346946164466587 1103 46-47 1.7065603746638303 1103 48-49 1.8442771853032554 1103 50-51 1.8238156465233075 1103 52-53 1.6525221484793846 1103 54-55 1.7134902549109796 1103 56-57 1.8304919535332687 1103 58-59 1.7362347394193236 1103 60-61 2.0485391369536714 1103 62-63 2.053781522629606 1103 64-65 2.0990639685886308 1103 66-67 1.9199594256184298 1103 68-69 1.7689223434812007 1103 70-71 1.8083488813948634 1103 72-73 1.6234709847204094 1103 74-75 1.8131888472475843 1103 76-77 1.600116679446355 1103 78-79 2.003999751139517 1103 80-81 1.6699695218349646 1103 82-83 1.8247092103377476 1103 84-85 1.7644738954961348 1103 86-87 1.9086642709644082 1103 88-89 1.9553468903234084 1103 90-91 2.056732092064056 1103 92-93 2.0977876359052345 1103 94-95 1.983675892215011 1103 96-97 1.415785275933807 1103 98-99 1.9258064307492582 1103 100-101 2.2322911136611907 1104 1 0.43859523097160036 1104 2 0.5376157785248097 1104 3 0.2213156335995805 1104 4 0.1005640052063228 1104 5 0.19066676607672406 1104 6 -0.11780090929149623 1104 7 0.06796140282014562 1104 8 0.3166061654221437 1104 9 0.3599538704792238 1104 10-11 0.5588775930116441 1104 12-13 0.5219222410853419 1104 14-15 0.7956485935446622 1104 16-17 0.6814641961835832 1104 18-19 0.32493924531081575 1104 20-21 0.3831017817800131 1104 22-23 0.5842114264690537 1104 24-25 0.3515909676329656 1104 26-27 0.5187213841147909 1104 28-29 0.42379644872288225 1104 30-31 0.7258605538779648 1104 32-33 0.7856200534621749 1104 34-35 0.9506957053856269 1104 36-37 0.9951441264092153 1104 38-39 0.8068295085582804 1104 40-41 0.9308340895518512 1104 42-43 0.8152819122038437 1104 44-45 0.8618321629150643 1104 46-47 0.7780004910368703 1104 48-49 1.050887820506098 1104 50-51 0.9007242182597395 1104 52-53 0.7031605839085131 1104 54-55 0.723214946468552 1104 56-57 0.8583676067847037 1104 58-59 1.1855262646604245 1104 60-61 1.1648636884325825 1104 62-63 1.2592876914782707 1104 64-65 0.8812093271361263 1104 66-67 0.835777538866644 1104 68-69 0.8559111910648483 1104 70-71 0.9913239366181799 1104 72-73 1.2844967915196115 1104 74-75 1.1299875111126845 1104 76-77 1.1421456649536026 1104 78-79 0.9292839491891911 1104 80-81 0.7956416300187925 1104 82-83 1.1210617775485652 1104 84-85 1.0910080262002957 1104 86-87 1.297059292784354 1104 88-89 1.376121633063562 1104 90-91 1.496622184192713 1104 92-93 1.346572722915635 1104 94-95 1.349507474565101 1104 96-97 1.7786428805887269 1104 98-99 2.445385117242445 1104 100-101 2.98499840313913 1105 1 0.2517871784800789 1105 2 0.525772684287908 1105 3 0.5038232005851739 1105 4 0.2501973365082506 1105 5 0.451296095509754 1105 6 0.5702564576006566 1105 7 0.5124532467269489 1105 8 0.07137488990260721 1105 9 0.694302906577299 1105 10-11 0.5634584124806423 1105 12-13 0.5392399732242055 1105 14-15 0.760870930879868 1105 16-17 0.8786527309307814 1105 18-19 0.8131164651292053 1105 20-21 0.8367247935418476 1105 22-23 0.5620894202529527 1105 24-25 0.7415497301007221 1105 26-27 0.7582342530688138 1105 28-29 0.7914503784768812 1105 30-31 1.0591837301836478 1105 32-33 0.6445084683564417 1105 34-35 1.0204440142405247 1105 36-37 1.273233386368183 1105 38-39 0.8901069782395581 1105 40-41 0.9974438768636986 1105 42-43 1.1834030652296192 1105 44-45 1.4745853268291782 1105 46-47 1.5435762929341976 1105 48-49 1.8660400743505292 1105 50-51 1.934293194634499 1105 52-53 1.5427811173556094 1105 54-55 1.3436874513947075 1105 56-57 1.3734000077029052 1105 58-59 1.2425782889560324 1105 60-61 1.5551677898403469 1105 62-63 1.276067101151824 1105 64-65 1.3122024812994937 1105 66-67 1.3583058261886407 1105 68-69 1.5984305402025143 1105 70-71 1.9912426803970007 1105 72-73 1.2121381265593278 1105 74-75 1.4597557277417685 1105 76-77 1.270560965500053 1105 78-79 0.561091696969882 1105 80-81 1.313091403517074 1105 82-83 1.3100739050395767 1105 84-85 1.5182633941894075 1105 86-87 1.2845255206674295 1105 88-89 1.4073544930794064 1105 90-91 1.4758815337366649 1105 92-93 1.6096194249287592 1105 94-95 1.4300527038092135 1105 96-97 1.2277358580198126 1105 98-99 1.894112441678221 1105 100-101 2.009791707626505 1106 1 0.36931880303099973 1106 2 0.3332780431020268 1106 3 0.2833419639822097 1106 4 0.07189223129434907 1106 5 0.37473298226517926 1106 6 0.2637020996016872 1106 7 0.28780572083859113 1106 8 0.4053120332773048 1106 9 0.24690985643087515 1106 10-11 0.4449606918635425 1106 12-13 0.4000466634394684 1106 14-15 0.6450601649468339 1106 16-17 0.31653577292576784 1106 18-19 0.7499428395518137 1106 20-21 0.8371776510728637 1106 22-23 0.6486304944310177 1106 24-25 0.3007683999072057 1106 26-27 0.43893950319712616 1106 28-29 0.5136524736977179 1106 30-31 0.6459965188803309 1106 32-33 0.6185597318289595 1106 34-35 0.6394549733927732 1106 36-37 0.6920058405540956 1106 38-39 1.1783507967342501 1106 40-41 0.6819380350015436 1106 42-43 0.8468393481750027 1106 44-45 1.1866283181041197 1106 46-47 1.4912108670971662 1106 48-49 1.4202320255626901 1106 50-51 1.4259455208126823 1106 52-53 1.6970092971702329 1106 54-55 1.852412506492378 1106 56-57 1.7422222762172268 1106 58-59 1.563578802194563 1106 60-61 1.4343906863171085 1106 62-63 1.6090381484603355 1106 64-65 1.5971102493156693 1106 66-67 1.6206160034068873 1106 68-69 1.6810770396137968 1106 70-71 1.8938481207254796 1106 72-73 2.1425037335393746 1106 74-75 1.8021311164444818 1106 76-77 2.285792073793381 1106 78-79 2.1314959477925726 1106 80-81 2.2727719880051964 1106 82-83 2.440723302739059 1106 84-85 2.289872246044613 1106 86-87 1.929922978627154 1106 88-89 2.1292999690326724 1106 90-91 2.734704707966049 1106 92-93 2.1543013679894685 1106 94-95 1.9475480997576504 1106 96-97 1.8868398039184342 1106 98-99 2.6094160071765167 1106 100-101 2.9745442964620565 1107 1 0.05678099603821707 1107 2 0.14361800358111765 1107 3 0.35883496716807883 1107 4 0.19702833646836382 1107 5 0.263469522975754 1107 6 0.3426229367997351 1107 7 0.17517112794428868 1107 8 0.4084575750767172 1107 9 0.1797243097921708 1107 10-11 0.37874300424003593 1107 12-13 0.3095921729769131 1107 14-15 0.14685473687085704 1107 16-17 0.21009144498287213 1107 18-19 0.6708405287884176 1107 20-21 0.514719289174252 1107 22-23 0.53486673268565 1107 24-25 0.3512382148041624 1107 26-27 0.727401817186724 1107 28-29 0.9084381332533127 1107 30-31 0.7117504407257158 1107 32-33 0.4290005109554613 1107 34-35 0.6842587803049014 1107 36-37 0.553158399331366 1107 38-39 0.2620706015493539 1107 40-41 0.6802846091839072 1107 42-43 0.7044632542926834 1107 44-45 0.8704610398043329 1107 46-47 1.2589247034483364 1107 48-49 1.2244422122788237 1107 50-51 1.552896361640208 1107 52-53 1.6854958076352133 1107 54-55 1.3241429577566421 1107 56-57 1.5442547801491529 1107 58-59 1.0551705269999943 1107 60-61 1.7865905492037584 1107 62-63 1.536347903672663 1107 64-65 1.5973925415284427 1107 66-67 1.8947822331927426 1107 68-69 2.201039131802375 1107 70-71 1.703260549648327 1107 72-73 1.625364563478474 1107 74-75 1.7503304954016166 1107 76-77 1.886671212398852 1107 78-79 1.8099449537207057 1107 80-81 1.79229047814513 1107 82-83 1.6742596176158564 1107 84-85 1.7460564618771777 1107 86-87 1.8194862322465397 1107 88-89 1.651580890111834 1107 90-91 1.8151579886048346 1107 92-93 1.1534948188101346 1107 94-95 1.5529237500379267 1107 96-97 1.33247838923117 1107 98-99 2.0970959684621526 1107 100-101 3.0920181844323764 1108 1 0.3964251566221577 1108 2 0.4074752116103113 1108 3 0.308937613153482 1108 4 0.4961843583661718 1108 5 0.3255703441436353 1108 6 0.321238356507763 1108 7 0.13211683962312293 1108 8 0.248500914492773 1108 9 0.3005614448286664 1108 10-11 0.4007833783276311 1108 12-13 0.4799845087433354 1108 14-15 0.7847139978197575 1108 16-17 0.4807061803843311 1108 18-19 0.8272045798833076 1108 20-21 0.8504286869844719 1108 22-23 0.9059034571382014 1108 24-25 0.4135101126143823 1108 26-27 0.6583153664567973 1108 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94-95 -1.0196328307819797 2316 96-97 -1.4353221892015497 2316 98-99 -1.2056401101312453 2316 100-101 -0.4654119216136223 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1277.0 3 217.0 4 218.0 5 229.0 6 222.0 7 265.0 8 326.0 9 427.0 10 534.0 11 679.0 12 942.0 13 1334.0 14 1494.0 15 2020.0 16 2783.0 17 4086.0 18 3365.0 19 2100.0 20 1898.0 21 1891.0 22 2046.0 23 2202.0 24 2504.0 25 2708.0 26 3133.0 27 3583.0 28 4167.0 29 5056.0 30 6037.0 31 7739.0 32 9968.0 33 13373.0 34 18642.0 35 27571.0 36 42094.0 37 61883.0 38 55448.0 39 7874.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.18162974951166 19.7929897755123 15.455127333507997 21.57025314146804 2 14.525386020696079 20.30384313153384 42.43956459319039 22.731206254579696 3 18.584358319795776 25.767993899811025 30.934920266551735 24.71272751384146 4 11.868935498735302 17.086188435056172 38.822090282543186 32.22278578366534 5 12.33330792000769 37.818011860288586 35.55103272020923 14.297647499494495 6 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52-53 20.59261223339907 28.170127438145272 30.242778781300235 20.994481547155427 54-55 20.51752761108221 28.018828223115438 30.30773971853761 21.15590444726474 56-57 20.495886113167916 28.345152372007348 30.03626051248412 21.122701002340616 58-59 20.626637909042415 27.793017993393697 30.223998038650514 21.356346058913374 60-61 20.597956008965863 27.85679140788207 30.554170401061594 20.991082182090476 62-63 20.46391726957798 27.797759770973773 30.85657862256233 20.881744336885912 64-65 20.313897779456493 27.691854031237366 30.740711289585114 21.253536899721023 66-67 20.18378716869356 27.93535144698848 30.439356363883352 21.4415050204346 68-69 20.152917105012467 27.81368301592956 30.703203306853105 21.330196572204873 70-71 20.302373663075027 27.80124713397744 30.545008150769355 21.351371052178177 72-73 20.28574457933521 27.652679849440702 30.42099612998993 21.640579441234163 74-75 20.08091344051106 27.901054989330827 30.25937363321891 21.758657936939205 76-77 20.13337862131241 27.77506234000779 30.36774391304708 21.72381512563272 78-79 20.199260354049166 27.64642139936242 30.27561090381289 21.878707342775527 80-81 20.117104011823063 27.902333796138283 30.18526557161934 21.795296620419307 82-83 20.354787241974044 27.663430935446087 30.353909137751355 21.627872684828514 84-85 19.956659178114876 27.453660443878935 30.32532124561787 22.264359132388318 86-87 20.136615462100227 27.418521120226906 30.461967686781797 21.982895730891066 88-89 20.194141300007786 27.052044381781403 30.445458024689316 22.3083562935215 90-91 19.89065334625612 27.339976970932018 30.62600959268372 22.14336009012815 92-93 19.823785626831768 27.47791107436757 30.522728439999135 22.17557485880152 94-95 19.523723531682457 27.381589432719405 30.531528382253782 22.563158653344356 96-97 19.73802570045076 27.506638813743795 30.185687993148314 22.569647492657133 98-99 19.78072587752722 27.653289774496876 29.858621535117656 22.70736281285825 100-101 19.9232992901457 27.424396789503763 30.08705447738323 22.565249442967307 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 402.0 1 248.5 2 89.5 3 85.5 4 81.5 5 91.0 6 152.5 7 322.0 8 562.5 9 686.0 10 645.0 11 576.0 12 593.0 13 625.0 14 631.0 15 686.0 16 706.5 17 733.5 18 807.5 19 833.5 20 877.5 21 1020.0 22 1213.5 23 1441.5 24 1774.5 25 2098.0 26 2526.0 27 3020.0 28 3370.5 29 3891.0 30 4599.0 31 5234.5 32 5936.0 33 6770.0 34 7795.0 35 8591.5 36 9415.5 37 10321.5 38 10679.0 39 10729.5 40 10623.5 41 10716.5 42 10937.0 43 11304.0 44 11560.5 45 11522.5 46 11230.0 47 11083.5 48 11466.5 49 11112.5 50 10167.0 51 9343.0 52 8622.0 53 8058.5 54 7321.5 55 6503.0 56 5694.5 57 4821.0 58 4161.5 59 3591.5 60 2938.0 61 2317.5 62 1765.5 63 1310.5 64 952.5 65 714.0 66 527.0 67 375.0 68 251.0 69 172.5 70 134.5 71 93.5 72 73.0 73 63.0 74 39.0 75 29.0 76 21.0 77 11.5 78 10.0 79 6.5 80 4.5 81 5.5 82 4.5 83 3.0 84 2.0 85 2.0 86 1.5 87 0.5 88 1.0 89 1.0 90 0.0 91 0.5 92 1.5 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2659301767906461 2 0.24211553409297634 3 0.23318504308135016 4 0.22557758777515005 5 0.21697785568988043 6 0.18952486480228886 7 0.1862172755387236 8 0.19250169513949758 9 0.19481700762399326 10-11 0.19779383796120195 12-13 0.19779383796120195 14-15 0.19217093621314105 16-17 0.1901863826550019 18-19 0.18869796748639756 20-21 0.18224816842244532 22-23 0.1748060925794235 24-25 0.17612912828484958 26-27 0.18307506573833662 28-29 0.19580928440306283 30-31 0.18969024426546713 32-33 0.1968015611821324 34-35 0.18902872641275406 36-37 0.1769560256007409 38-39 0.18042899432748444 40-41 0.17447533365306694 42-43 0.184563480906941 44-45 0.18009823540112788 46-47 0.17860982023252353 48-49 0.18704417285461492 50-51 0.19729769957166718 52-53 0.20507053434104552 54-55 0.1832404452015149 56-57 0.16256801230423207 58-59 0.1660409810309756 60-61 0.17315229794764087 62-63 0.17712140506391916 64-65 0.17100236492632345 66-67 0.17149850331585825 68-69 0.1777829229166322 70-71 0.1729869184844626 72-73 0.1743099541898887 74-75 0.1756329898953148 76-77 0.18605189607554534 78-79 0.18820182909686275 80-81 0.18257892734880182 82-83 0.18142127110655398 84-85 0.17894057915888006 86-87 0.178279061306167 88-89 0.18009823540112788 90-91 0.17943671754841484 92-93 0.17976747647477137 94-95 0.17712140506391916 96-97 0.16918319083136257 98-99 0.17232540063174956 100-101 0.16951394975771908 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 302335.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.55772368588399 #Duplication Level Percentage of deduplicated Percentage of total 1 87.82601828665517 62.846299489878646 2 7.287007256171963 10.428833034683697 3 1.8478533209884271 3.966845320659988 4 0.8672974007842152 2.48247311035209 5 0.47548475146799196 1.7012303231198893 6 0.3275061562450647 1.406135702040617 7 0.2574796963148354 1.289726267453562 8 0.16756328473077015 0.9592357782930843 9 0.14571786739187687 0.9384515001831786 >10 0.755553439097503 9.774802530365584 >50 0.03373753729466894 1.5870762194772685 >100 0.007394528722118682 0.9660032584065078 >500 0.0 0.0 >1k 0.0013864741353972526 1.6528874650858356 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2175 0.719400664825442 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1753 0.5798203979029884 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1070 0.3539120512014818 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 475 0.1571104900193494 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 392 0.1296574991317578 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 305 0.10088147253874014 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.653794631782625E-4 0.0 22-23 0.0 0.0 0.0 3.30758926356525E-4 0.0 24-25 0.0 0.0 0.0 6.6151785271305E-4 0.0 26-27 0.0 0.0 0.0 0.0014884151686043626 0.0 28-29 0.0 0.0 0.0 0.004134486579456563 0.0 30-31 0.0 0.0 0.0 0.008268973158913126 0.0 32-33 0.0 0.0 0.0 0.015214910612400153 0.0 34-35 0.0 0.0 0.0 0.02298774538177849 0.0 36-37 0.0 0.0 0.0 0.03357203102518729 0.0 38-39 0.0 0.0 0.0 0.05176377197479617 0.0 40-41 0.0 0.0 0.0 0.07127854862983114 0.0 42-43 0.0 0.0 0.0 0.09922767790695752 0.0 44-45 0.0 0.0 0.0 0.12138852597284469 0.0 46-47 0.0 0.0 0.0 0.1721600211685713 0.0 48-49 0.0 0.0 0.0 0.23665801180809365 0.0 50-51 0.0 0.0 0.0 0.26378024376932874 0.0 52-53 0.0 0.0 0.0 0.3041328327848248 0.0 54-55 0.0 0.0 0.0 0.3787189706782212 0.0 56-57 0.0 0.0 0.0 0.5141647510212182 0.0 58-59 0.0 0.0 0.0 0.6682984107033589 0.0 60-61 0.0 0.0 0.0 0.8641076951064217 0.0 62-63 0.0 0.0 0.0 1.0653745017943672 0.0 64-65 0.0 0.0 0.0 1.2682951031140952 0.0 66-67 0.0 0.0 0.0 1.51818347197645 0.0 68-69 0.0 0.0 0.0 1.806770635222518 0.0 70-71 0.0 0.0 0.0 2.0194486248697636 0.0 72-73 0.0 0.0 0.0 2.3014206095887015 0.0 74-75 0.0 0.0 0.0 2.6072072370053085 0.0 76-77 0.0 0.0 0.0 2.962276944449038 0.0 78-79 0.0 0.0 0.0 3.31999272330362 0.0 80-81 0.0 0.0 0.0 3.894355598921726 0.0 82-83 0.0 0.0 0.0 4.457968809433245 0.0 84-85 0.0 0.0 0.0 5.255593960342004 0.0 86-87 0.0 0.0 0.0 6.050242280913556 0.0 88-89 0.0 0.0 0.0 6.96264077926803 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 935 0.0 49.29986 1 TGAACCG 45 9.6085534E-4 42.226868 5 GTATCAA 1610 0.0 36.30486 1 AACGTTG 55 0.0025773263 34.54352 8 CCTACGA 45 2.4106279E-5 31.667522 84-85 GTGCTAA 80 4.4742643E-4 29.700623 1 GGTACGA 40 4.4926032E-4 29.68584 18-19 GTACATG 1425 0.0 29.679781 1 TAGGACA 180 2.0918378E-10 29.035788 4 CCAGTAC 100 4.9318645E-5 28.507868 3 ATCAACG 2030 0.0 28.32062 3 TATCAAC 2055 0.0 28.207294 2 TCAACGC 2085 0.0 27.573557 4 CATGGGG 555 0.0 27.394945 4 TACATGG 1505 0.0 27.150349 2 GAACGTT 70 0.008384207 27.14134 7 CAACGCA 2145 0.0 27.019289 5 AACGCAG 2170 0.0 26.703577 6 AGACGAT 45 8.945512E-4 26.387413 76-77 ACGCTCA 45 8.949846E-4 26.385223 40-41 >>END_MODULE