FastQCFastQC Report
Thu 26 May 2016
SRR937673_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937673_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences302335
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25890.8563348603370433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22090.7306464683215638No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14280.47232374683711775No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5880.19448624869763673No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT4170.13792647229067093No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3440.11378107066664463No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3210.10617361536044453No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA15600.040.1956671
GGCTCGG550.002577665734.542639
GTATCAA25700.032.716741
CTATACC753.071615E-431.6693134
ACCCCTA902.408474E-531.6640768
ATTGGCG300.00395106631.66407476-77
TAAGGTG1551.3478711E-930.6477225
GTATAGG1252.1434607E-730.402541
ATAAGCC804.4836994E-429.6899813
TAGGACC1153.849682E-628.915464
CTAAGGG1205.3609947E-627.7106482
GTAAGGT1205.3609947E-627.7106484
GACCCCT1205.3678996E-627.7060667
TCAACGC30850.026.7931614
ATCAACG30900.026.749813
GTGTAGA1606.204937E-826.7209841
TATCAAC31200.026.6448552
CAACGCA31200.026.6448555
AACGCAG31350.026.3614796
TCACGCC559.474615E-525.90697130-31
Produced by FastQC (version 0.11.5)