##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937673_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 302335 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.486420692278436 33.0 31.0 34.0 31.0 34.0 2 32.69337655249971 34.0 31.0 34.0 31.0 34.0 3 32.74853060346966 34.0 31.0 34.0 31.0 34.0 4 36.2826831164106 37.0 35.0 37.0 35.0 37.0 5 36.233016356028905 37.0 35.0 37.0 35.0 37.0 6 36.29039310698398 37.0 37.0 37.0 35.0 37.0 7 36.25106917822945 37.0 37.0 37.0 35.0 37.0 8 36.23177601005507 37.0 37.0 37.0 35.0 37.0 9 37.96274992971373 39.0 38.0 39.0 35.0 39.0 10-11 37.890335224171864 39.0 38.0 39.0 35.0 39.0 12-13 37.80685497874874 39.0 38.0 39.0 35.0 39.0 14-15 39.063255990871056 40.0 38.0 41.0 36.0 41.0 16-17 39.03129806340648 40.0 38.0 41.0 36.0 41.0 18-19 39.0262672201366 40.0 38.0 41.0 36.0 41.0 20-21 38.95094514363206 40.0 38.0 41.0 35.0 41.0 22-23 38.89760861296244 40.0 38.0 41.0 35.0 41.0 24-25 38.902781682570655 40.0 38.0 41.0 35.0 41.0 26-27 38.72608364893247 40.0 38.0 41.0 35.0 41.0 28-29 38.68799510476789 40.0 38.0 41.0 35.0 41.0 30-31 38.669566871185935 40.0 38.0 41.0 35.0 41.0 32-33 38.495501678601556 40.0 38.0 41.0 35.0 41.0 34-35 38.333019663618174 40.0 38.0 41.0 34.5 41.0 36-37 38.15696164850249 40.0 38.0 41.0 34.0 41.0 38-39 37.99728777680387 40.0 37.5 41.0 34.0 41.0 40-41 37.79980485223345 40.0 37.0 41.0 33.0 41.0 42-43 37.55716010385831 40.0 37.0 41.0 33.0 41.0 44-45 37.160323812988906 40.0 37.0 41.0 32.5 41.0 46-47 36.671563001306495 40.0 36.5 41.0 31.0 41.0 48-49 36.19654026163031 40.0 36.0 41.0 30.0 41.0 50-51 35.88403261283014 40.0 36.0 41.0 28.5 41.0 52-53 35.634906643293036 39.0 35.5 41.0 28.0 41.0 54-55 35.41449220235832 39.0 35.0 41.0 27.0 41.0 56-57 35.134984371640726 39.0 35.0 40.0 26.0 41.0 58-59 34.85478360097243 38.5 35.0 40.0 26.0 41.0 60-61 34.50723865910331 38.0 34.5 40.0 24.5 41.0 62-63 34.10767360709147 38.0 34.0 40.0 23.0 41.0 64-65 33.732204342864705 37.5 34.0 40.0 22.0 41.0 66-67 33.31248780326459 37.0 33.0 39.5 20.5 41.0 68-69 32.827732482180366 36.0 33.0 39.0 18.5 40.5 70-71 32.398918418310814 36.0 32.0 39.0 15.5 40.0 72-73 31.922269998511585 35.0 32.0 38.0 10.5 40.0 74-75 31.415553938511916 35.0 31.0 37.5 7.5 39.5 76-77 30.186903600310913 34.0 29.5 36.0 7.0 38.5 78-79 30.75267666661154 34.5 31.0 36.0 4.0 39.0 80-81 30.545176707956408 35.0 31.0 36.0 2.0 38.5 82-83 30.155603883109794 34.0 31.0 35.5 2.0 37.0 84-85 29.743132617791524 34.0 30.0 35.0 2.0 37.0 86-87 29.409902922255114 34.0 30.0 35.0 2.0 36.0 88-89 29.038164618717648 34.0 29.5 35.0 2.0 36.0 90-91 28.567106024773842 34.0 29.0 35.0 2.0 36.0 92-93 28.140941339904412 34.0 29.0 35.0 2.0 35.0 94-95 27.673220103527544 34.0 27.5 35.0 2.0 35.0 96-97 27.213736418211585 33.5 27.0 35.0 2.0 35.0 98-99 26.483784543635373 33.0 25.5 35.0 2.0 35.0 100-101 24.11293267402054 31.0 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.18227636482896514 1101 2 0.2105636433777036 1101 3 0.2265998552424051 1101 4 0.10769381842368375 1101 5 0.06972915811514468 1101 6 0.0806397773521823 1101 7 0.010979572904162183 1101 8 0.13512835620586117 1101 9 0.08093748532218825 1101 10-11 0.14647503511672966 1101 12-13 0.1310951088711363 1101 14-15 0.11105397697186703 1101 16-17 0.1661611140694177 1101 18-19 0.06416228796988577 1101 20-21 0.01742311109715189 1101 22-23 -0.0388113019021219 1101 24-25 -0.2111234882761508 1101 26-27 -0.21626433820114244 1101 28-29 -0.01116192458471943 1101 30-31 0.19001665748125873 1101 32-33 0.25760348666579347 1101 34-35 0.2686191911582725 1101 36-37 0.10522731241677263 1101 38-39 0.08409137389636356 1101 40-41 0.070565871338097 1101 42-43 0.10651285653766962 1101 44-45 0.37586647532742745 1101 46-47 0.4423731882716808 1101 48-49 0.414530897717853 1101 50-51 0.5940277368213032 1101 52-53 0.5429848858888136 1101 54-55 0.6200855495327602 1101 56-57 0.910011257298649 1101 58-59 0.8489781281567659 1101 60-61 0.8536404038870913 1101 62-63 0.7865137069258097 1101 64-65 0.8158616677247679 1101 66-67 0.7966773119526351 1101 68-69 0.7745634832806942 1101 70-71 0.6016792497007231 1101 72-73 0.5462837696237877 1101 74-75 0.865317596877567 1101 76-77 0.8454291326164807 1101 78-79 0.8202327984329898 1101 80-81 0.8918068902457428 1101 82-83 0.9002129365611715 1101 84-85 1.09216142977008 1101 86-87 1.2363865943879162 1101 88-89 1.3908465299201147 1101 90-91 0.8546025391855281 1101 92-93 1.2013140450847004 1101 94-95 0.9000908609048537 1101 96-97 0.9983685413742691 1101 98-99 1.2880160672731336 1101 100-101 1.4774386815762384 1102 1 0.08466756521156071 1102 2 0.05855503505632953 1102 3 0.16763285380768167 1102 4 0.07132366060446316 1102 5 -0.15867830243004732 1102 6 -0.04255486635895522 1102 7 -0.12443410476202388 1102 8 0.053181919094434704 1102 9 -0.1654996261077457 1102 10-11 -0.0972002398713201 1102 12-13 -0.09817318381178808 1102 14-15 0.0615919970579526 1102 16-17 -0.011768106399259182 1102 18-19 0.043155592608819404 1102 20-21 0.11821220722340797 1102 22-23 -0.07540862375768853 1102 24-25 -0.1257337226137878 1102 26-27 -0.23756993361003254 1102 28-29 -0.12665690306391042 1102 30-31 -0.06703738364738854 1102 32-33 -0.16719326130169065 1102 34-35 -0.19881265963081773 1102 36-37 -0.31766843124655253 1102 38-39 -0.22200618707925202 1102 40-41 -0.053202660464862106 1102 42-43 -0.01386017072201895 1102 44-45 -0.07855724538036668 1102 46-47 -0.18382241191961413 1102 48-49 -0.1880037746876937 1102 50-51 -0.2527441426621948 1102 52-53 -0.1460634163589134 1102 54-55 0.12972208707461164 1102 56-57 0.22578122382184063 1102 58-59 0.0762736805240607 1102 60-61 0.14268870613481255 1102 62-63 -0.007795236163623542 1102 64-65 0.15028299723217486 1102 66-67 0.17379352429122719 1102 68-69 0.16218902130077595 1102 70-71 0.007848546687810654 1102 72-73 0.2534932387773061 1102 74-75 0.05061171452462787 1102 76-77 -0.07371242644617126 1102 78-79 0.17716727954250544 1102 80-81 0.401598855812459 1102 82-83 0.23391690595380865 1102 84-85 0.10989218060700168 1102 86-87 0.3677710994094383 1102 88-89 -0.04141076324105342 1102 90-91 0.17311043014679228 1102 92-93 0.1781791813831255 1102 94-95 0.2310688618613348 1102 96-97 -0.1360527881331457 1102 98-99 -0.002957151282583226 1102 100-101 0.7763387294002442 1103 1 0.16667534181106447 1103 2 0.2987989374953486 1103 3 0.31807470605229327 1103 4 0.21195639301874536 1103 5 0.19547510866927809 1103 6 0.08225955569830745 1103 7 0.37653797017611623 1103 8 0.22234063242069624 1103 9 0.24828616392406389 1103 10-11 0.2463045321329176 1103 12-13 0.36152989147983305 1103 14-15 0.45649302215516485 1103 16-17 0.44719265712141976 1103 18-19 0.2284419128633246 1103 20-21 0.29585448364617406 1103 22-23 0.43808554379267406 1103 24-25 0.39812629859511617 1103 26-27 0.3073929508014146 1103 28-29 0.10077328428143772 1103 30-31 0.4352425739347936 1103 32-33 0.46629913883970175 1103 34-35 0.5472636924370491 1103 36-37 0.46993319476970896 1103 38-39 0.5130768811427373 1103 40-41 0.25961105462881306 1103 42-43 0.18511473207048823 1103 44-45 0.5595834403743112 1103 46-47 0.8501736997806333 1103 48-49 0.655067982116833 1103 50-51 0.6855025876311913 1103 52-53 0.633010461336383 1103 54-55 0.6513728470604718 1103 56-57 0.9990138148434085 1103 58-59 0.9840591170740751 1103 60-61 0.7215858429322708 1103 62-63 0.5618760257663951 1103 64-65 0.7457849413820554 1103 66-67 0.8881095370165681 1103 68-69 1.014972690441823 1103 70-71 1.167834407415981 1103 72-73 1.1122684243552463 1103 74-75 1.1484378014811512 1103 76-77 1.0072790899907318 1103 78-79 1.3113666432752744 1103 80-81 1.2756091067845325 1103 82-83 1.1540918794426709 1103 84-85 1.3150088295995772 1103 86-87 1.2597028774228676 1103 88-89 1.2853478086924923 1103 90-91 1.7849520703023245 1103 92-93 1.098884377565522 1103 94-95 1.255291201910822 1103 96-97 1.465546716992339 1103 98-99 1.4207526401631618 1103 100-101 1.7323832681065028 1104 1 0.11522337262837112 1104 2 -0.06576116163724066 1104 3 0.04468426372698531 1104 4 -0.09025792733037719 1104 5 0.07308946134423877 1104 6 0.060310307274193065 1104 7 0.013195477853500392 1104 8 0.26925620809011264 1104 9 0.3139938305368588 1104 10-11 0.1531737740918686 1104 12-13 0.05637391928005542 1104 14-15 0.09839270428435754 1104 16-17 -0.03002301013788866 1104 18-19 -0.15667742337933532 1104 20-21 -0.22570685464378926 1104 22-23 -0.19513464908485645 1104 24-25 -0.23707654607919437 1104 26-27 0.14520880124050706 1104 28-29 0.09303663216140734 1104 30-31 -0.08381889904618589 1104 32-33 0.1727212066162238 1104 34-35 -0.02089535071795723 1104 36-37 0.08145735833848278 1104 38-39 -0.04242659388554415 1104 40-41 0.3072485741590043 1104 42-43 -0.02212625710035354 1104 44-45 -0.03489961549551879 1104 46-47 -0.1964671062104273 1104 48-49 -0.3622640581827241 1104 50-51 -0.4678135052517334 1104 52-53 -0.14078944168608132 1104 54-55 -0.35913305240615045 1104 56-57 -0.28041078512426765 1104 58-59 -0.4374968335751319 1104 60-61 -0.14697188563838637 1104 62-63 -0.37308976271483374 1104 64-65 -0.20592563455674195 1104 66-67 0.10934222922065118 1104 68-69 -0.2570277405694412 1104 70-71 -0.4724004937208086 1104 72-73 -0.3947031827779881 1104 74-75 0.3427430652061645 1104 76-77 0.10328684819341305 1104 78-79 0.08777416442176289 1104 80-81 0.02285871618058266 1104 82-83 -0.07408548169088647 1104 84-85 -0.4334745495287038 1104 86-87 0.2647888102928633 1104 88-89 0.15331828442117157 1104 90-91 0.05888127670284149 1104 92-93 0.036888795430940036 1104 94-95 -0.1474672701542623 1104 96-97 0.2678634025157507 1104 98-99 0.18934269457833253 1104 100-101 0.7398667011111897 1105 1 0.25006807167564205 1105 2 0.22338910142978108 1105 3 0.283719752381586 1105 4 0.1400628316027266 1105 5 0.2609538447110964 1105 6 0.15196732476459118 1105 7 0.05199532346668434 1105 8 0.19649126202392608 1105 9 0.14384330338712203 1105 10-11 0.3401748101086852 1105 12-13 0.35951040941758805 1105 14-15 0.1201185864222083 1105 16-17 0.4453157267341581 1105 18-19 0.5650301761087491 1105 20-21 0.6750894563237679 1105 22-23 0.4310054772685632 1105 24-25 0.4926855763333009 1105 26-27 0.35925800188243784 1105 28-29 0.5500724052127808 1105 30-31 0.4652497015185375 1105 32-33 0.5117018473215253 1105 34-35 0.46448868649739694 1105 36-37 0.25681844067135273 1105 38-39 -0.1612638173604708 1105 40-41 0.005940348998016987 1105 42-43 -0.32733478087795476 1105 44-45 -0.2706137561094124 1105 46-47 -0.2740363263538441 1105 48-49 -0.20043213667623405 1105 50-51 -0.16524580866246197 1105 52-53 -0.11300997107036892 1105 54-55 -0.3036045822576554 1105 56-57 0.26758117050983543 1105 58-59 0.24825488804105333 1105 60-61 -0.15812430199526517 1105 62-63 -0.21457918924905073 1105 64-65 -0.16424119309169072 1105 66-67 -0.3267395957940735 1105 68-69 -0.1251793646781607 1105 70-71 -0.3554727914436029 1105 72-73 -0.1106111194793904 1105 74-75 -0.28955221990160496 1105 76-77 -0.31916424571846136 1105 78-79 -0.3713133282143879 1105 80-81 -0.5793920811460929 1105 82-83 -0.7597356330305978 1105 84-85 -0.7280892934700383 1105 86-87 -0.5733691111729975 1105 88-89 -0.7426635954415062 1105 90-91 -0.5398044039195824 1105 92-93 -0.048846674941010804 1105 94-95 0.3132859750160719 1105 96-97 0.5992364295131303 1105 98-99 0.28727675515484563 1105 100-101 0.2587790865906996 1106 1 -0.3446546285628642 1106 2 -0.33619185440151966 1106 3 -0.2693702455050797 1106 4 -0.09340032088949357 1106 5 -0.2147578963751613 1106 6 -0.26873732155367236 1106 7 -0.3537046433237663 1106 8 -0.11978443764095914 1106 9 -0.36942542227178876 1106 10-11 -0.42311655842677 1106 12-13 -0.5451479850515142 1106 14-15 -0.47851378383411003 1106 16-17 -0.5973585479390309 1106 18-19 -0.40662094244935076 1106 20-21 -0.4925433064090754 1106 22-23 -0.2863359471193263 1106 24-25 -0.14655301920454633 1106 26-27 -0.8305043988662959 1106 28-29 -0.7843175956929329 1106 30-31 -0.7413288088820806 1106 32-33 -0.7322675998903847 1106 34-35 -0.7601588393909608 1106 36-37 -0.22896183285460125 1106 38-39 -0.6864426960852228 1106 40-41 -1.221228083813024 1106 42-43 -1.2460166635576613 1106 44-45 -0.9064106344115004 1106 46-47 -0.6595388472607695 1106 48-49 -0.6372132253457394 1106 50-51 -1.3400639108875083 1106 52-53 -1.019283557400101 1106 54-55 -1.3695255435232383 1106 56-57 -1.342734924046809 1106 58-59 -1.2873397138971754 1106 60-61 -2.166411594812942 1106 62-63 -1.0121971586358782 1106 64-65 -1.659395617509773 1106 66-67 -1.8587717184882706 1106 68-69 -1.5806634693788233 1106 70-71 -1.270999766657198 1106 72-73 -1.1915669507748667 1106 74-75 -1.3669757624551195 1106 76-77 -1.504971690696273 1106 78-79 -1.6232077822191613 1106 80-81 -1.2197628205114874 1106 82-83 -1.3344420303979874 1106 84-85 -1.4890107075931525 1106 86-87 -1.206539415795163 1106 88-89 -1.8320274815629318 1106 90-91 -1.610027511729868 1106 92-93 -2.100955481510468 1106 94-95 -1.8001095507515181 1106 96-97 -2.563022423851603 1106 98-99 -2.9190904217313083 1106 100-101 -1.7991564868778838 1107 1 0.27320237026764005 1107 2 0.21948020270216517 1107 3 -0.1141901834007939 1107 4 0.00205477448494662 1107 5 0.07564013564483929 1107 6 0.003110079341595906 1107 7 0.14740436179782535 1107 8 0.053018724031829834 1107 9 -0.20460709747442962 1107 10-11 0.1169487720567517 1107 12-13 -0.028028978720108455 1107 14-15 0.07189553987439723 1107 16-17 -0.07404927665621841 1107 18-19 0.23303304795270918 1107 20-21 0.03746032306222702 1107 22-23 0.023762048605966868 1107 24-25 0.10837701265414523 1107 26-27 -0.33038341648938996 1107 28-29 -0.07814346172511222 1107 30-31 -0.11494255248009466 1107 32-33 0.03619229445167349 1107 34-35 -0.20491022060642905 1107 36-37 0.29475071301788347 1107 38-39 -0.12345120517905883 1107 40-41 -0.6232726859826343 1107 42-43 -0.46217184361977104 1107 44-45 -0.6269816712304674 1107 46-47 -0.6269340966860923 1107 48-49 -0.39254653179410326 1107 50-51 -0.28763821423065394 1107 52-53 -0.09342559071058787 1107 54-55 -0.007465616919219542 1107 56-57 -0.26706044729559153 1107 58-59 -0.05963071684108456 1107 60-61 -0.02308780564489865 1107 62-63 -0.5027806969671289 1107 64-65 -0.14473658617533403 1107 66-67 0.057568967885082145 1107 68-69 0.6289788260601341 1107 70-71 0.1274556916643661 1107 72-73 0.22866533538878286 1107 74-75 0.09501560746866389 1107 76-77 0.12597156856896063 1107 78-79 0.8393683358596391 1107 80-81 1.3121160358017754 1107 82-83 1.5760108469662093 1107 84-85 1.3688753270078067 1107 86-87 1.1719741140673179 1107 88-89 0.749877587598661 1107 90-91 0.6292554660497061 1107 92-93 0.6961973998864792 1107 94-95 0.10477813727525742 1107 96-97 0.6030057247147589 1107 98-99 0.3667909348844134 1107 100-101 0.9289557847678793 1108 1 0.22872244326033808 1108 2 0.39671560416201856 1108 3 0.4789895611247559 1108 4 0.23722813214917693 1108 5 0.1331789130171046 1108 6 0.3819878165678716 1108 7 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0.4912275791925538 2316 54-55 0.21098455064264243 2316 56-57 -0.2551015802892138 2316 58-59 -0.08703741013402322 2316 60-61 0.19407695586637175 2316 62-63 0.6726772289383902 2316 64-65 0.4711335878135614 2316 66-67 0.6507838602323233 2316 68-69 0.21248431198953455 2316 70-71 0.37078764777693607 2316 72-73 0.23318721024532252 2316 74-75 -0.029576964720657628 2316 76-77 0.30301329835824475 2316 78-79 0.6625561428651032 2316 80-81 -0.1918779044268426 2316 82-83 -0.34000163983542464 2316 84-85 -0.13199691281223735 2316 86-87 0.12634219927137735 2316 88-89 0.5711831928871867 2316 90-91 0.15186484033240077 2316 92-93 0.2896418290284686 2316 94-95 0.38375715761221585 2316 96-97 0.5965557407823319 2316 98-99 0.5153930558043953 2316 100-101 1.5742755295229642 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 1.0 5 6.0 6 9.0 7 27.0 8 70.0 9 119.0 10 207.0 11 390.0 12 660.0 13 994.0 14 1317.0 15 2021.0 16 2984.0 17 4672.0 18 4543.0 19 2385.0 20 1740.0 21 1756.0 22 1748.0 23 1807.0 24 2014.0 25 2250.0 26 2620.0 27 2990.0 28 3641.0 29 4415.0 30 5436.0 31 6828.0 32 8551.0 33 11566.0 34 16617.0 35 25338.0 36 41484.0 37 70270.0 38 64276.0 39 6583.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.24708934823905 20.582395107325112 15.638092968765513 20.532422575670328 2 15.403443200423371 21.28334463426332 41.00517637719748 22.30803578811583 3 19.22238576413581 26.30691120776622 31.137314568276913 23.33338845982106 4 12.462665586187507 18.14940380703524 38.04422246845387 31.343708138323382 5 12.58724603547399 38.75576402717776 34.393627648806174 14.263362288542073 6 30.240296359998016 38.054145236244565 17.11412836753932 14.591430036218103 7 26.946268212413383 34.970810524749034 20.96614682388741 17.116774438950173 8 26.29764995782824 35.73883275174889 19.376188664891593 18.58732862553128 9 26.70415267832041 17.26660823258968 21.17254039393388 34.856698695156034 10-11 24.5343741214216 28.345213091438303 26.822895132882397 20.297517654257692 12-13 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32.079752856112776 19.950121388927478 38-39 20.43494798815883 27.502273967618702 32.444804604164254 19.617973440058215 40-41 20.577373736705535 27.48412767977191 32.242929602807486 19.69556898071507 42-43 20.646652187074565 27.601488100653565 31.75348080902653 19.998378903245335 44-45 20.73676550845916 27.790034233548877 31.262506821902857 20.210693436089105 46-47 21.074966510658708 28.209271172705776 30.607273388790578 20.108488927844938 48-49 21.151041063720708 28.471893760232852 30.22177386012205 20.15529131592439 50-51 21.27176807184084 28.542180032083614 29.77376089437214 20.41229100170341 52-53 21.18874758132535 28.912299270676566 29.57084029305241 20.328112854945672 54-55 20.758099459209156 28.929002596457572 29.678833082507815 20.634064861825458 56-57 21.100104189061803 29.117039046091257 29.661798997800453 20.121057767046487 58-59 21.226288719466815 28.439479385449918 29.633684489060148 20.70054740602312 60-61 21.075793407974597 28.733524070980863 29.998676964294575 20.19200555674996 62-63 21.113996063968777 28.63396563414755 30.20655894950965 20.04547935237402 64-65 20.86063472637968 28.61329320125027 29.842393371591115 20.683678700778938 66-67 20.733457919195594 28.848462797889756 29.77425703276167 20.643822250152976 68-69 20.892552962773085 28.603370433459574 29.807994443250035 20.696082160517307 70-71 20.856996378189756 28.941902194585477 29.58969355185473 20.611407875370034 72-73 20.88511088693006 28.63991929482197 29.62607703375395 20.848892784494023 74-75 20.770337539484345 29.013346122678485 29.06329072055832 21.153025617278846 76-77 20.903633386806025 28.67398746423669 29.55827145385086 20.86410769510642 78-79 20.957712471265317 28.506292688573932 29.100666479236608 21.435328360924142 80-81 20.80060198124597 29.099178064068003 28.8595432219227 21.240676732763326 82-83 20.929963335312376 28.772270448790994 29.136767388438965 21.160998827457668 84-85 20.6854978748739 28.65314965187623 29.097028131046688 21.564324342203182 86-87 20.64349149122662 28.663072419666925 29.208328509765657 21.4851075793408 88-89 20.485388724428198 28.473216795938285 29.212462996345113 21.828931483288404 90-91 20.473833156081447 28.69231112750392 29.181228611927533 21.65262710448709 92-93 20.428441248509486 28.549739769819915 29.092692220548177 21.929126761122422 94-95 20.07706682984107 28.857889427290917 28.993335207633912 22.071708535234094 96-97 20.425190599831314 28.988043064812214 28.64951130368631 21.937255031670166 98-99 20.165446162191483 29.30004564488281 28.328934225062348 22.20557396786336 100-101 19.984881991872 29.434133286578877 28.08856440122598 22.492420320323134 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 196.0 1 129.5 2 79.0 3 102.0 4 98.5 5 98.0 6 154.0 7 337.0 8 569.0 9 719.5 10 759.5 11 694.5 12 712.5 13 796.5 14 838.5 15 877.5 16 899.0 17 904.5 18 949.0 19 995.5 20 1027.5 21 1144.0 22 1323.5 23 1479.5 24 1676.0 25 2048.5 26 2467.0 27 2894.0 28 3269.0 29 3845.5 30 4549.5 31 5123.5 32 5837.0 33 6615.0 34 7537.0 35 8330.5 36 9324.0 37 10108.5 38 10483.5 39 10790.5 40 10762.5 41 10771.0 42 10741.5 43 10955.5 44 11348.5 45 11369.5 46 11033.5 47 10968.0 48 11417.5 49 11051.0 50 10135.5 51 9351.0 52 8466.5 53 7942.0 54 7446.5 55 6709.5 56 5903.5 57 5082.0 58 4287.0 59 3713.0 60 3109.5 61 2393.0 62 1826.5 63 1364.0 64 986.5 65 707.5 66 501.0 67 383.0 68 291.0 69 185.5 70 122.5 71 90.0 72 64.0 73 46.0 74 36.0 75 26.0 76 20.0 77 13.0 78 8.5 79 8.0 80 4.0 81 1.5 82 3.0 83 3.0 84 1.0 85 0.0 86 0.5 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05589825855425273 2 0.0 3 0.0 4 0.0 5 0.009592008864339226 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0046306249689913506 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.30758926356525E-4 22-23 0.0019845535581391504 24-25 0.0 26-27 0.0016537946317826252 28-29 0.0014884151686043628 30-31 0.0 32-33 0.0 34-35 0.0 36-37 3.30758926356525E-4 38-39 0.0 40-41 1.653794631782625E-4 42-43 0.023318504308135014 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.653794631782625E-4 84-85 0.0 86-87 0.0 88-89 0.0 90-91 0.011411182959300114 92-93 0.002149933021317413 94-95 0.0 96-97 0.0 98-99 3.30758926356525E-4 100-101 0.015380290075578415 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 302335.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.73201160963251 #Duplication Level Percentage of deduplicated Percentage of total 1 86.28275551645747 58.44104598351782 2 8.145832163491718 11.03467197335478 3 2.2223313090498036 4.5156891007503335 4 0.9781659273307628 2.6501258378445676 5 0.5599403240449978 1.896294226445859 6 0.36506465203234995 1.483593794983295 7 0.23319975889696953 1.105656214388113 8 0.16843114134602163 0.9126544016857913 9 0.1365888494223053 0.8326293781336137 >10 0.8298784227610425 10.289515204758008 >50 0.05712774986909591 2.666868314813744 >100 0.018714262888152013 1.8983299036403949 >500 4.924806023197899E-4 0.1961374070181958 >1k 0.0014774418069593695 2.076788258665454 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2589 0.8563348603370433 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2209 0.7306464683215638 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1428 0.47232374683711775 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 588 0.19448624869763673 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 417 0.13792647229067093 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 344 0.11378107066664463 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 321 0.10617361536044453 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.653794631782625E-4 0.0 22-23 0.0 0.0 0.0 3.30758926356525E-4 0.0 24-25 0.0 0.0 0.0 6.6151785271305E-4 0.0 26-27 0.0 0.0 0.0 0.0014884151686043626 0.0 28-29 0.0 0.0 0.0 0.004299866042634826 0.0 30-31 0.0 0.0 0.0 0.008930491011626176 0.0 32-33 0.0 0.0 0.0 0.01620718739146973 0.0 34-35 0.0 0.0 0.0 0.023980022160848064 0.0 36-37 0.0 0.0 0.0 0.03456430780425687 0.0 38-39 0.0 0.0 0.0 0.05275604875386575 0.0 40-41 0.0 0.0 0.0 0.07177468701936593 0.0 42-43 0.0 0.0 0.0 0.09955843683331403 0.0 44-45 0.0 0.0 0.0 0.12155390543602296 0.0 46-47 0.0 0.0 0.0 0.17282153902128433 0.0 48-49 0.0 0.0 0.0 0.23715415019762848 0.0 50-51 0.0 0.0 0.0 0.2644417616220418 0.0 52-53 0.0 0.0 0.0 0.3049597301007161 0.0 54-55 0.0 0.0 0.0 0.3803727653100038 0.0 56-57 0.0 0.0 0.0 0.51780309921114 0.0 58-59 0.0 0.0 0.0 0.6721021383564589 0.0 60-61 0.0 0.0 0.0 0.8685729406122347 0.0 62-63 0.0 0.0 0.0 1.0709974035424281 0.0 64-65 0.0 0.0 0.0 1.2744141432516911 0.0 66-67 0.0 0.0 0.0 1.5238063737245109 0.0 68-69 0.0 0.0 0.0 1.8127242958969356 0.0 70-71 0.0 0.0 0.0 2.0255676650073595 0.0 72-73 0.0 0.0 0.0 2.309524203284436 0.0 74-75 0.0 0.0 0.0 2.618122281575074 0.0 76-77 0.0 0.0 0.0 2.9768303372087255 0.0 78-79 0.0 0.0 0.0 3.3378537053268724 0.0 80-81 0.0 0.0 0.0 3.91486265235583 0.0 82-83 0.0 0.0 0.0 4.479798898572775 0.0 84-85 0.0 0.0 0.0 5.284535366398201 0.0 86-87 0.0 0.0 0.0 6.087121901202309 0.0 88-89 0.0 0.0 0.0 7.009277787884301 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 1560 0.0 40.195667 1 GGCTCGG 55 0.0025776657 34.54263 9 GTATCAA 2570 0.0 32.71674 1 CTATACC 75 3.071615E-4 31.669313 4 ACCCCTA 90 2.408474E-5 31.664076 8 ATTGGCG 30 0.003951066 31.664074 76-77 TAAGGTG 155 1.3478711E-9 30.647722 5 GTATAGG 125 2.1434607E-7 30.40254 1 ATAAGCC 80 4.4836994E-4 29.689981 3 TAGGACC 115 3.849682E-6 28.91546 4 CTAAGGG 120 5.3609947E-6 27.710648 2 GTAAGGT 120 5.3609947E-6 27.710648 4 GACCCCT 120 5.3678996E-6 27.706066 7 TCAACGC 3085 0.0 26.793161 4 ATCAACG 3090 0.0 26.74981 3 GTGTAGA 160 6.204937E-8 26.720984 1 TATCAAC 3120 0.0 26.644855 2 CAACGCA 3120 0.0 26.644855 5 AACGCAG 3135 0.0 26.361479 6 TCACGCC 55 9.474615E-5 25.906971 30-31 >>END_MODULE