FastQCFastQC Report
Thu 26 May 2016
SRR937672_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937672_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189031
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14150.7485544699017622No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11730.6205331400669731No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7500.3967603197359163No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.15817511413471866No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2510.13278245367162No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8800.039.4581831
CCCTATA600.00392102231.7022782
TAGCGTC300.00394638431.66869524-25
GTAGCGT300.00394638431.66869524-25
GTATCAA13000.030.7349721
AGTATGG650.005799912429.2636432
GTACATG10750.027.8759061
ATGGGAC1554.4954504E-827.6116623
TGCTATA700.008328319527.1733822
GTAGACA700.00833910127.1661824
CGTGGTA350.00836030127.15898330-31
GGCGGAA350.00837652627.14819144-45
GGACAGG700.00838233627.1374116
ATGGGTA908.888873E-426.4045665
CATGGGT1457.7706136E-726.2294164
TACATGG11700.026.0121272
ACATGGG11400.025.8623873
ATACAGA1309.978869E-625.5717936
CCCCTAT950.001212892625.0347521
GCTTTGC950.001212892625.0347521
Produced by FastQC (version 0.11.5)