FastQCFastQC Report
Thu 26 May 2016
SRR937672_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937672_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences189031
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15690.830022588887537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14110.7464384148631706No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8880.469764218567325No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3750.19838015986795815No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT3100.16399426549084542No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2680.1417756875856341No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2320.12273119223831011No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTCG200.002071434271.24969
TAATACC608.264739E-539.5831154
GTTGTAG756.866918E-638.0199051
ATAAGAG500.001612445137.999798
GCTACAC500.001612445137.999793
GCCGTAT250.001614488137.9997992-93
GGTATCA10300.037.8353461
ACTGTCC1404.5110937E-1033.9283838
GTAAGGT600.003942784431.6664924
TATAAGA953.4757257E-529.9998322
GTTTAGA700.00835277627.1570761
GGTAAGG700.00837437827.1427083
GTATCAA14350.027.1427062
CATAGAC1000.00164860923.7498683
ATTGGTC500.001651653623.74986820-21
TGCACTG1201.7035233E-423.7498685
TACAAGG1201.7035233E-423.7498682
TGGGATG2052.6397174E-823.1706036
GTACATG9200.022.7292921
AGTACTT11500.022.09770212-13
Produced by FastQC (version 0.11.5)