FastQCFastQC Report
Thu 26 May 2016
SRR937671_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937671_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309813
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70210.5360307158350085No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54200.41379952710806805No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34850.26606851512391466No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16740.12780450339094207No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51350.035.653831
GTATCAA78650.033.859081
ATCAACG95600.027.5997163
TCAACGC96600.027.4145324
CAACGCA97100.027.2243985
TATCAAC98950.026.809452
AACGCAG100000.026.5076586
GTACATG65400.023.9950731
TACATGG63500.023.7331312
GAGTACT58100.023.1391512-13
CGCAGAG111150.023.1210928
ACGCAGA112850.022.9850187
ACATGGG64400.022.5155963
GTACTTT61450.021.56847614-15
GCAGAGT116100.021.1533369
GTAGGAC2509.396899E-920.9180973
AGTACTT61000.020.83200612-13
AGAGTAC93650.020.49324610-11
CATGGGA43100.019.9665994
ATGGGAT19150.019.3654925
Produced by FastQC (version 0.11.5)