FastQCFastQC Report
Thu 26 May 2016
SRR937671_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937671_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1309813
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96260.7349140678860264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81220.6200885164523485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53360.40738639790565523No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19550.1492579475085375No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16880.12887335825801088No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58900.039.8652881
GTATCAA92700.034.5591771
ATCAACG117050.027.2406963
TATCAAC118250.027.044632
TCAACGC118200.026.9354654
CAACGCA119250.026.7381425
AACGCAG123000.025.911086
GTACATG66450.023.8194921
TACATGG65500.023.5055712
CGCAGAG135700.023.2078538
ACGCAGA136350.023.1668937
GAGTACT81950.022.80851712-13
ACATGGG64800.022.0728593
GTACTTT84500.021.64074114-15
AGTACTT84300.021.60921912-13
CCGTCCG550.002879318821.58964218-19
GCAGAGT143700.021.5522259
AGAGTAC119350.021.19329510-11
CATGGGA42350.020.421384
TCTACAC6050.020.421383
Produced by FastQC (version 0.11.5)