FastQCFastQC Report
Thu 26 May 2016
SRR937670_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937670_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313012
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69560.5297742899531763No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52850.4025096495690824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34370.26176455356082046No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16110.12269499440979977No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13280.1011414975643787No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGACG500.001610029538.0281565
GTATCAA77250.034.5312541
GGTATCA50800.034.1646581
ATCAACG94000.028.0617053
TCAACGC95050.027.7069994
TATCAAC96650.027.3916912
CAACGCA96100.027.3537585
AACGCAG98800.026.6380736
GTACATG64600.026.5719031
TACATGG63250.026.5264822
GGACCGA1658.401548E-825.9124536
ACATGGG63150.025.0622333
CGCAGAG109550.023.5037358
ACGCAGA110600.023.452417
GAGTACT57650.022.86505312-13
GCAGAGT114200.021.7979249
CATGGGA42600.021.7598974
CATGGGG20450.021.3858434
GTGTAAG5650.021.03971
AGAGTAC93350.020.76168610-11
Produced by FastQC (version 0.11.5)