FastQCFastQC Report
Thu 26 May 2016
SRR937670_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937670_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1313012
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94150.7170536141330011No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78980.6015177317495956No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52770.4019003634391765No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19540.14881813722951504No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18090.1377748261249707No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA64500.036.3985521
GTATCAA97850.032.9295461
TATAGCG804.488481E-429.6937145
ATCAACG122800.025.9989243
TATCAAC123900.025.9214862
TCAACGC123400.025.8725134
CAACGCA124850.025.5720315
AACGCAG127750.025.0220496
GAGTACT81650.023.26960812-13
GTACATG65400.023.035541
TACATGG65150.022.8981152
ACGCAGA142850.022.3114367
CGCAGAG142150.022.2876478
AGTACTT84600.022.12132312-13
GTACTTT86000.021.98129814-15
CGTAAAC1100.002874089221.5954283
ACATGGG65700.021.549413
AGAGTAC120450.021.27501510-11
GCAGAGT147950.020.9644499
TAAGGTG5100.019.5629185
Produced by FastQC (version 0.11.5)