##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937670_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1313012 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.359777366848135 33.0 31.0 34.0 31.0 34.0 2 32.55953106293012 34.0 31.0 34.0 31.0 34.0 3 32.63940618973779 34.0 31.0 34.0 31.0 34.0 4 36.23589959573865 37.0 35.0 37.0 35.0 37.0 5 36.17334571199654 37.0 35.0 37.0 35.0 37.0 6 36.22104748471453 37.0 36.0 37.0 35.0 37.0 7 36.177403557621716 37.0 36.0 37.0 35.0 37.0 8 36.160192747667196 37.0 36.0 37.0 35.0 37.0 9 37.853591589414265 39.0 38.0 39.0 35.0 39.0 10-11 37.80133768769821 39.0 37.5 39.0 35.0 39.0 12-13 37.71349652554585 39.0 37.0 39.0 35.0 39.0 14-15 38.9439022644119 40.0 38.0 41.0 36.0 41.0 16-17 38.91778940329563 40.0 38.0 41.0 35.5 41.0 18-19 38.939811669657246 40.0 38.0 41.0 35.5 41.0 20-21 38.85478464781738 40.0 38.0 41.0 35.0 41.0 22-23 38.69816612490975 40.0 38.0 41.0 35.0 41.0 24-25 38.77321151672643 40.0 38.0 41.0 35.0 41.0 26-27 38.65169206374352 40.0 38.0 41.0 35.0 41.0 28-29 38.50580306958352 40.0 38.0 41.0 34.0 41.0 30-31 38.506481281206874 40.0 38.0 41.0 35.0 41.0 32-33 38.39289206800852 40.0 38.0 41.0 34.0 41.0 34-35 38.24712036142853 40.0 38.0 41.0 34.0 41.0 36-37 38.08011465241749 40.0 37.5 41.0 34.0 41.0 38-39 37.924578754801935 40.0 37.0 41.0 33.5 41.0 40-41 37.73334897167734 40.0 37.0 41.0 33.0 41.0 42-43 37.483551940119355 40.0 37.0 41.0 33.0 41.0 44-45 37.10117691232068 40.0 37.0 41.0 32.5 41.0 46-47 36.64659005401322 40.0 36.5 41.0 31.0 41.0 48-49 36.22358935028774 40.0 36.0 41.0 30.0 41.0 50-51 35.92811337596305 39.0 36.0 41.0 29.0 41.0 52-53 35.686124726963655 39.0 35.5 41.0 28.0 41.0 54-55 35.4682744712158 39.0 35.0 40.0 28.0 41.0 56-57 35.214590194149025 39.0 35.0 40.0 27.0 41.0 58-59 34.96422881131322 38.0 35.0 40.0 26.5 41.0 60-61 34.621011841475934 38.0 34.0 40.0 26.0 41.0 62-63 34.28409755584869 38.0 34.0 40.0 25.5 41.0 64-65 33.902968518185666 37.0 34.0 40.0 24.5 41.0 66-67 33.47938366138314 36.5 33.0 39.0 23.0 41.0 68-69 33.038929956466504 36.0 33.0 39.0 22.0 40.0 70-71 32.615181734820396 35.5 32.0 39.0 20.5 40.0 72-73 32.12378485497467 35.0 32.0 38.0 19.0 40.0 74-75 31.613283046918077 35.0 31.0 37.0 18.0 39.0 76-77 30.388307189880976 34.0 29.5 36.0 16.5 38.5 78-79 30.962707500007618 34.0 31.0 36.0 16.0 39.0 80-81 30.7620878560135 34.0 31.0 36.0 12.0 37.5 82-83 30.40251802725337 34.0 31.0 35.5 7.5 37.0 84-85 30.018499830923098 34.0 30.5 35.0 6.0 37.0 86-87 29.6125496187392 34.0 30.0 35.0 2.0 36.0 88-89 29.256709001897924 34.0 29.5 35.0 2.0 36.0 90-91 28.745027463572306 34.0 29.0 35.0 2.0 35.5 92-93 28.288978699356896 34.0 29.0 35.0 2.0 35.0 94-95 27.830267735557634 33.5 27.5 35.0 2.0 35.0 96-97 27.300890624000388 33.0 27.0 35.0 2.0 35.0 98-99 26.457802365858043 33.0 25.5 34.5 2.0 35.0 100-101 23.829131416925357 30.5 12.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.26975838242881167 1101 2 0.14556950152461923 1101 3 0.16527996843871762 1101 4 0.09931488075478967 1101 5 0.09428196505734121 1101 6 0.047833354687831786 1101 7 0.11467229397026557 1101 8 0.04139106047455954 1101 9 0.11107366526800178 1101 10-11 0.16086740387251552 1101 12-13 0.2890288533530665 1101 14-15 0.3209328543541048 1101 16-17 0.19873939854414147 1101 18-19 0.28453149962403046 1101 20-21 0.266302123839786 1101 22-23 0.2484340330648891 1101 24-25 0.22043197701089667 1101 26-27 0.15418241545359734 1101 28-29 0.17435211837727138 1101 30-31 0.3860150203896211 1101 32-33 0.3728416347783181 1101 34-35 0.2648903228417936 1101 36-37 0.18812815001205507 1101 38-39 0.12819427805971628 1101 40-41 0.3163903563721604 1101 42-43 0.3706040900824945 1101 44-45 0.4631919278911951 1101 46-47 0.4055265467515454 1101 48-49 0.34491081549720803 1101 50-51 0.30224283366657545 1101 52-53 0.23937294362125527 1101 54-55 0.43588854176792324 1101 56-57 0.38774488994380363 1101 58-59 0.3912998144175148 1101 60-61 0.45138653721657107 1101 62-63 0.5950655710812711 1101 64-65 0.4897969161163118 1101 66-67 0.48056331864982127 1101 68-69 0.4482538196245187 1101 70-71 0.38358700957405745 1101 72-73 0.43142325349074184 1101 74-75 0.3025373621450207 1101 76-77 0.22432014548534696 1101 78-79 0.24905087104103885 1101 80-81 0.22058104195178885 1101 82-83 0.22990136198952982 1101 84-85 0.29780036581194835 1101 86-87 0.04521782170018085 1101 88-89 0.33660399229388815 1101 90-91 0.4366319271606045 1101 92-93 0.5455187206376202 1101 94-95 0.5337064215828029 1101 96-97 0.6227013552793323 1101 98-99 0.6543665325757644 1101 100-101 1.180842992408575 1102 1 0.1082838494866536 1102 2 0.15196358824172052 1102 3 0.1311729683749121 1102 4 0.07929971605419439 1102 5 0.013407069085559442 1102 6 0.04485302263926627 1102 7 0.048044342318100064 1102 8 0.08401038576078435 1102 9 0.1428794865037446 1102 10-11 0.11533325207074085 1102 12-13 0.10259382737486078 1102 14-15 0.22850522608884205 1102 16-17 0.23611743267218088 1102 18-19 0.12604171102022832 1102 20-21 0.1732352226655749 1102 22-23 0.07986318051547414 1102 24-25 0.09757074963405898 1102 26-27 0.1373624273735743 1102 28-29 0.02559312579127493 1102 30-31 0.21930089332382607 1102 32-33 0.154426162710827 1102 34-35 0.25829072524262386 1102 36-37 0.2882182286051176 1102 38-39 0.24022010964058182 1102 40-41 0.20196797262241972 1102 42-43 0.2734753367512113 1102 44-45 0.3245957963153572 1102 46-47 0.41351737326166926 1102 48-49 0.26068648246572224 1102 50-51 0.17276775360048902 1102 52-53 0.2742003348447355 1102 54-55 0.31307211610278785 1102 56-57 0.17337328146732034 1102 58-59 0.15955345727184067 1102 60-61 0.16171530032276138 1102 62-63 0.1635763825488823 1102 64-65 0.26326113380375915 1102 66-67 0.17436008043640072 1102 68-69 0.2762074403495163 1102 70-71 0.11502094266202789 1102 72-73 0.21998989060638507 1102 74-75 0.20025692107895665 1102 76-77 0.14860847708317948 1102 78-79 0.3885152054844596 1102 80-81 0.2512697053538133 1102 82-83 0.43333609200491097 1102 84-85 0.322061850323454 1102 86-87 0.37594049367426763 1102 88-89 0.22803060776893957 1102 90-91 0.3191623482016581 1102 92-93 0.35136133901256983 1102 94-95 0.48176579471181924 1102 96-97 0.3491312426369717 1102 98-99 0.13094389223440217 1102 100-101 0.3958431824764439 1103 1 0.17718788884143777 1103 2 0.2133782486999607 1103 3 0.19756682500288036 1103 4 0.12042592468716151 1103 5 0.07714093253695609 1103 6 0.11182248684772134 1103 7 0.10690269520367224 1103 8 0.13372915762005277 1103 9 0.2243554053316359 1103 10-11 0.060668638785635665 1103 12-13 0.05549140162728605 1103 14-15 0.24153149247316463 1103 16-17 0.24784438883261117 1103 18-19 0.10933670228127568 1103 20-21 0.11141820869659824 1103 22-23 0.263610843300917 1103 24-25 0.1677153948634711 1103 26-27 0.20311301394057324 1103 28-29 0.19918150537969836 1103 30-31 0.28476291370643025 1103 32-33 0.3850059825189689 1103 34-35 0.4490520329772991 1103 36-37 0.32589994048493764 1103 38-39 0.36526075477850384 1103 40-41 0.2720236083352532 1103 42-43 0.30075894546767046 1103 44-45 0.44479066425916614 1103 46-47 0.4440735588512723 1103 48-49 0.40710218168597834 1103 50-51 0.49980720559157277 1103 52-53 0.454976947641903 1103 54-55 0.5787190170658789 1103 56-57 0.41474841178906274 1103 58-59 0.40036365353115144 1103 60-61 0.3553561707481592 1103 62-63 0.4566100060367191 1103 64-65 0.48120693901586975 1103 66-67 0.4382201201190199 1103 68-69 0.5319388336438848 1103 70-71 0.568967761858346 1103 72-73 0.5808830734685273 1103 74-75 0.6414652776811955 1103 76-77 0.5613692397060426 1103 78-79 0.5205435741706417 1103 80-81 0.5557154162196447 1103 82-83 0.5660195478613339 1103 84-85 0.6848572065807055 1103 86-87 0.7720475185645306 1103 88-89 0.7099889314929335 1103 90-91 0.690365196506189 1103 92-93 0.8541385502891394 1103 94-95 0.8336684446032727 1103 96-97 1.0420533659809053 1103 98-99 1.2233117550339543 1103 100-101 1.5552958215990778 1104 1 0.15918949177083164 1104 2 0.15416544879407468 1104 3 0.03540282130683181 1104 4 0.03328342818591068 1104 5 -0.01808336728436899 1104 6 0.03043239902974193 1104 7 0.10024882256058021 1104 8 0.05395131284929988 1104 9 0.12465172668221669 1104 10-11 0.07317957620522009 1104 12-13 0.1982891261538242 1104 14-15 0.26656954366396945 1104 16-17 0.2392324423518275 1104 18-19 0.08228297963708542 1104 20-21 -0.022149191543100244 1104 22-23 0.013189645417575946 1104 24-25 0.05423755278878417 1104 26-27 0.04698818152512274 1104 28-29 0.08879898368255112 1104 30-31 0.10491619689307896 1104 32-33 0.137704614636867 1104 34-35 0.18286918628255222 1104 36-37 0.1421691836032437 1104 38-39 0.233717114168023 1104 40-41 0.28383945282659084 1104 42-43 0.32672395062694193 1104 44-45 0.3365296955898103 1104 46-47 0.37275741656800676 1104 48-49 0.5175440166724812 1104 50-51 0.605463291453681 1104 52-53 0.7303638933261283 1104 54-55 0.49280641348208576 1104 56-57 0.5073773875564029 1104 58-59 0.5385766078476664 1104 60-61 0.4465449610266674 1104 62-63 0.36825673412450755 1104 64-65 0.4966134433798075 1104 66-67 0.39320304916611093 1104 68-69 0.34186964297616385 1104 70-71 0.5377447071259027 1104 72-73 0.5934925395753936 1104 74-75 0.5194963215705819 1104 76-77 0.5045037264622714 1104 78-79 0.4966604164487656 1104 80-81 0.5193789186938922 1104 82-83 0.5956967806267812 1104 84-85 0.5522692109042033 1104 86-87 0.4908407627318532 1104 88-89 0.6193726860241497 1104 90-91 0.4911377398453318 1104 92-93 0.4685480031608087 1104 94-95 0.38834500335406474 1104 96-97 0.613051373348501 1104 98-99 0.672722666228232 1104 100-101 0.9217235194346358 1105 1 0.10958358424829129 1105 2 0.14396482530153065 1105 3 0.07461652572327182 1105 4 0.007979724951987066 1105 5 0.061452560829671654 1105 6 0.05692719896447329 1105 7 0.14555938302343208 1105 8 0.12534246491000545 1105 9 0.1680445091514926 1105 10-11 0.15612925484693818 1105 12-13 0.19282084942565803 1105 14-15 0.17660111466303618 1105 16-17 0.05939203477610988 1105 18-19 0.22570542391549253 1105 20-21 0.16006680324064604 1105 22-23 0.07072034274754202 1105 24-25 0.00460786296939375 1105 26-27 0.03954762570641179 1105 28-29 -0.0516668586632818 1105 30-31 0.14004198872795115 1105 32-33 0.08828533208269818 1105 34-35 0.06570702206710877 1105 36-37 0.08242475080771783 1105 38-39 0.02558997665150997 1105 40-41 0.21290809183840764 1105 42-43 0.10394163329315376 1105 44-45 0.14574612836419476 1105 46-47 -0.07902430686898754 1105 48-49 0.1077413502806408 1105 50-51 -0.0012194679005190778 1105 52-53 -0.0913729278131683 1105 54-55 -0.1846887953760188 1105 56-57 -0.1386660640211872 1105 58-59 -0.1281263928836367 1105 60-61 -0.059041566566413906 1105 62-63 -0.2005322382886945 1105 64-65 -0.2918856360471196 1105 66-67 -0.05828833206344797 1105 68-69 -0.24100274320077375 1105 70-71 -0.20043136246638227 1105 72-73 -0.3207673256186254 1105 74-75 -0.2923649007998428 1105 76-77 -0.20259680857334672 1105 78-79 0.1462741853416496 1105 80-81 -0.014335459884602386 1105 82-83 -0.033814010920725224 1105 84-85 0.0673808786972252 1105 86-87 0.26031598653317545 1105 88-89 0.4169159747590676 1105 90-91 0.28697002444543784 1105 92-93 0.406112537414252 1105 94-95 0.6022799759482744 1105 96-97 0.7168878542027599 1105 98-99 0.4966471283684655 1105 100-101 0.601806704624078 1106 1 -0.07542480108814686 1106 2 -0.010275421159015252 1106 3 0.04721215974057458 1106 4 0.04218436220951105 1106 5 0.024596509981414272 1106 6 0.02791148149685796 1106 7 -0.05506132463938229 1106 8 0.02195661150706485 1106 9 -0.10548393878143969 1106 10-11 -0.04059625813062695 1106 12-13 -0.16322036051617772 1106 14-15 0.030961752474333082 1106 16-17 0.1751653170088332 1106 18-19 0.10956588560843983 1106 20-21 0.12281820999693593 1106 22-23 0.15078015289021351 1106 24-25 0.21782234708281578 1106 26-27 0.12952175996642978 1106 28-29 0.17116359982273366 1106 30-31 0.09138907506449812 1106 32-33 0.07630709365174937 1106 34-35 0.05333142208183261 1106 36-37 0.11377337927510212 1106 38-39 0.08597535164340542 1106 40-41 -0.1654493285271812 1106 42-43 -0.3420355957430701 1106 44-45 -0.36894310598536606 1106 46-47 -0.1598849735952399 1106 48-49 -0.2884714918139295 1106 50-51 -0.4770680341569431 1106 52-53 -0.29749010522305497 1106 54-55 -0.30745423902779123 1106 56-57 -0.574710130409926 1106 58-59 -0.6657438708337935 1106 60-61 -0.4247451900481636 1106 62-63 -0.4861484509905907 1106 64-65 -0.42098476877376356 1106 66-67 -0.4832798766301565 1106 68-69 -0.4864556477516331 1106 70-71 -0.30580971901054 1106 72-73 -0.3953367994891437 1106 74-75 -0.42465037375209747 1106 76-77 -0.5603755443414933 1106 78-79 -0.3310047948892816 1106 80-81 -0.4283756849656051 1106 82-83 -0.5702512330524065 1106 84-85 -0.4046149890930941 1106 86-87 -0.37443996249939815 1106 88-89 -0.6797953436050825 1106 90-91 -0.5958350414543716 1106 92-93 -0.6860784099272372 1106 94-95 -0.5686845042222011 1106 96-97 -0.3590428129966412 1106 98-99 -0.22052695191696614 1106 100-101 -0.13849802393460564 1107 1 0.3307615425275756 1107 2 0.33867878852174726 1107 3 0.3691331444720092 1107 4 0.14813036494089715 1107 5 0.27970247263889547 1107 6 0.26877891223230677 1107 7 0.26405397492836613 1107 8 0.22071698549593322 1107 9 0.3324309887447683 1107 10-11 0.3893327157572841 1107 12-13 0.28787360047070365 1107 14-15 0.3845972286337016 1107 16-17 0.40526160709874404 1107 18-19 0.5064569558428076 1107 20-21 0.42774750416854346 1107 22-23 0.42106820323368055 1107 24-25 0.38975191541725707 1107 26-27 0.447561546888096 1107 28-29 0.5402899171495719 1107 30-31 0.4224592838125929 1107 32-33 0.35276637022056434 1107 34-35 0.37649638868264645 1107 36-37 0.30224688156449986 1107 38-39 0.5652741353340502 1107 40-41 0.4601454382934733 1107 42-43 0.5861862660532324 1107 44-45 0.42952094152936127 1107 46-47 0.5054602025479795 1107 48-49 0.1437795388590004 1107 50-51 0.2166286546221059 1107 52-53 0.19606194343957384 1107 54-55 0.22308224897182072 1107 56-57 0.10308767577488709 1107 58-59 0.20524713816233486 1107 60-61 0.2568639921010174 1107 62-63 0.2566233865922598 1107 64-65 0.37949130090971295 1107 66-67 0.37146954621862704 1107 68-69 0.4113892273249107 1107 70-71 0.33197888862656555 1107 72-73 0.377641633888004 1107 74-75 0.34781560571387615 1107 76-77 0.3878012727560183 1107 78-79 0.5522684532229682 1107 80-81 0.6052489784162312 1107 82-83 0.632606209956311 1107 84-85 0.6901768136941442 1107 86-87 0.7723268376615131 1107 88-89 0.8253452096914096 1107 90-91 1.1072389133318197 1107 92-93 1.0771710859462296 1107 94-95 1.3638842478756565 1107 96-97 1.64455845686415 1107 98-99 1.9716381402350578 1107 100-101 2.102441790915794 1108 1 0.13314310337909774 1108 2 0.1534718828520596 1108 3 0.031257877281326785 1108 4 0.029421625312764377 1108 5 -0.08554109742747329 1108 6 0.020994360617791585 1108 7 0.14639996451992943 1108 8 0.12962619753638904 1108 9 0.21613749039703123 1108 10-11 0.16533422842216083 1108 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-0.3519985969035204 2316 62-63 -0.16872304999402132 2316 64-65 -0.35013635768228113 2316 66-67 -0.36061872411504226 2316 68-69 -0.31003062010908167 2316 70-71 -0.18858912422888352 2316 72-73 -0.031037520349581627 2316 74-75 -0.0073805967593187916 2316 76-77 -0.15568223312221008 2316 78-79 0.029858011871301215 2316 80-81 -0.058524559877653815 2316 82-83 -0.13085933339847955 2316 84-85 -0.27883148416051284 2316 86-87 -0.16011860521397026 2316 88-89 0.1363179334379545 2316 90-91 0.17015276710117888 2316 92-93 0.22739769541603394 2316 94-95 0.04407109967791101 2316 96-97 0.26975355110968025 2316 98-99 0.3264932163795322 2316 100-101 0.9210959176785671 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 9.0 5 15.0 6 35.0 7 109.0 8 245.0 9 491.0 10 969.0 11 1706.0 12 2930.0 13 4311.0 14 5596.0 15 8370.0 16 12251.0 17 18519.0 18 16700.0 19 8701.0 20 6379.0 21 6323.0 22 6537.0 23 6707.0 24 7409.0 25 8726.0 26 10525.0 27 12826.0 28 15814.0 29 20233.0 30 25587.0 31 32828.0 32 43229.0 33 58623.0 34 82481.0 35 123593.0 36 195018.0 37 302516.0 38 243926.0 39 22769.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.985598768982314 20.49652062297417 15.653959100656264 20.863921507387246 2 15.424839986230133 21.552963720057395 40.396660502721986 22.625535790990487 3 19.261286263948843 25.900067935403488 31.06772824620034 23.770917554447333 4 12.558919917654917 17.747985355796715 38.08064060392487 31.612454122623497 5 12.831539198127834 38.62968241864306 34.275308276129486 14.263470107099627 6 30.428282452864103 37.80087310702416 17.36374077312317 14.407103666988572 7 26.906684782774263 34.699454384270666 20.908719798448146 17.48514103450692 8 26.01210042253993 35.45900570596461 19.727923278690522 18.80097059280494 9 26.41643793049873 17.051710113845115 21.110926632810667 35.420925322845484 10-11 24.47464303448864 27.902220238657378 27.09247897201244 20.53065775484154 12-13 25.61208464460781 25.444221201975363 28.22059419393559 20.72309995948124 14-15 22.724125902885884 26.33273724840291 27.442018808662834 23.50111804004838 16-17 20.651372569329148 29.813436587022814 28.179102704316488 21.356088139331554 18-19 20.44394872247931 27.64464452723966 31.71391426734866 20.197492482932372 20-21 21.69694564863078 26.470816717592832 32.05446713358294 19.777770500193448 22-23 21.298512379070107 26.505585480606474 31.879109141071705 20.316792999251714 24-25 21.011270270187936 26.55904896527983 32.164214797732235 20.265465966799997 26-27 20.744853428382115 26.87150135947175 32.150288268939306 20.23335694320683 28-29 20.689215274480095 27.091802326046334 32.11365875336681 20.10532364610676 30-31 20.83389184561908 26.77397464760414 32.02575452471112 20.366378982065665 32-33 20.917935251162977 26.874011814058058 31.83013559662821 20.377917338150755 34-35 21.218998760102725 26.833303884503724 31.85523056910371 20.092466786289844 36-37 21.324824144790753 26.572757903202714 31.674958035417806 20.427459916588727 38-39 20.796923713158645 27.200637618177755 31.730757879881477 20.271680788782124 40-41 21.153906204184157 27.11730607973642 31.5821160836892 20.146671632390227 42-43 21.030096370390147 27.05176990088738 31.174143500207002 20.743990228515475 44-45 21.250110433111043 27.18280564077099 31.020775133814464 20.546308792303495 46-47 21.43015448449824 27.685961742923904 30.193554971317855 20.690328801260005 48-49 21.613968493814223 27.785427703631036 29.89907936865771 20.701524433897024 50-51 21.63396069495176 28.166079213289745 29.526348578687777 20.67361151307071 52-53 21.687548052701747 28.362165906391528 29.081390376245754 20.868895664660972 54-55 21.423452337069275 28.378872394159384 29.19702942547364 21.0006458432977 56-57 21.579315345175825 28.604612905289517 29.1176699070534 20.698401842481257 58-59 21.67325965033069 28.04940091941277 29.381376560153292 20.895962870103244 60-61 21.517510883373493 28.067222538712517 29.658754070792952 20.75651250712103 62-63 21.361305151818872 27.98249368627248 29.91164589508702 20.744555266821628 64-65 21.4689203145135 28.163527827620765 29.57044566233972 20.79710619552601 66-67 21.352250151655184 28.25523614987378 29.30328485317912 21.08922884529191 68-69 21.459683125274655 28.217090336638464 29.3473931292274 20.975833408859483 70-71 21.53373312658224 28.207472589740227 29.226160918559767 21.03263336511776 72-73 21.596908482176858 27.995060212701787 29.1867858024146 21.221245502706754 74-75 21.43449564817382 28.707429939711137 28.542656121954714 21.315418290160334 76-77 21.46895839489662 28.2691628103932 28.993870581533148 21.26800821317703 78-79 21.587388386397077 28.315201993584218 28.654003162195014 21.44340645782369 80-81 21.456666601929992 28.66741837371569 28.512507316196395 21.363407708157926 82-83 21.519768251754694 28.604243868797347 28.5831848475582 21.292803031889758 84-85 21.42615058762242 28.543689542217344 28.481732334198778 21.548427535961455 86-87 21.185983067938448 28.6025946449842 28.501605468952302 21.709816818125045 88-89 21.4844571108261 28.508307616381266 28.483707688886316 21.523527583906315 90-91 21.397749457473463 28.30468930310571 28.551482702753344 21.746078536667483 92-93 21.183449348445574 28.180457289568615 28.780278078303823 21.855815283681988 94-95 21.124254766902357 28.286260902413684 28.71653115127661 21.872953179407347 96-97 21.105328816492158 28.25952847346407 28.740369471109172 21.894773238934604 98-99 20.91064738546934 28.395211231777267 28.437709683782575 22.25643169897082 100-101 20.851682652570734 28.613320901122023 28.50038736393699 22.034609082370256 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 361.0 1 264.0 2 205.5 3 270.0 4 266.0 5 270.5 6 472.0 7 1068.5 8 1824.5 9 2294.5 10 2329.5 11 2274.0 12 2522.5 13 2833.0 14 3006.0 15 3114.0 16 3277.0 17 3432.5 18 3570.5 19 3694.5 20 3836.5 21 4199.5 22 4896.0 23 5908.0 24 7006.0 25 8284.5 26 10278.5 27 12655.0 28 14549.0 29 16626.5 30 19173.5 31 21735.0 32 24226.5 33 26905.5 34 30387.5 35 33616.5 36 38023.0 37 41254.5 38 42294.5 39 43115.5 40 43328.0 41 45060.5 42 45957.5 43 46818.5 44 48603.0 45 49178.0 46 49498.5 47 50907.0 48 53396.5 49 51288.0 50 46224.5 51 42535.0 52 39256.5 53 37308.0 54 35482.0 55 32237.5 56 27761.0 57 23796.5 58 21307.5 59 19021.5 60 15476.0 61 11830.0 62 9066.5 63 6436.0 64 4321.0 65 3008.0 66 2149.5 67 1570.5 68 1115.0 69 786.0 70 565.5 71 406.0 72 319.5 73 258.0 74 192.0 75 157.5 76 111.0 77 53.5 78 29.5 79 20.0 80 12.0 81 9.5 82 5.0 83 2.5 84 2.0 85 1.5 86 1.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.021096532247991642 2 0.0 3 0.0 4 7.616076623823697E-5 5 0.023533676767615225 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0030464306495294785 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.235272792632513E-4 24-25 0.0 26-27 1.5232153247647393E-4 28-29 8.758488117397252E-4 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 3.808038311911848E-5 40-41 8.377684286206067E-4 42-43 0.016184162825625355 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.808038311911848E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.808038311911848E-5 68-69 7.616076623823697E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.808038311911848E-5 82-83 0.0027037072014574125 84-85 6.854468961441328E-4 86-87 0.0 88-89 0.0 90-91 0.013175812559214996 92-93 0.0027798679676956494 94-95 0.0 96-97 0.0 98-99 0.0017516976234794503 100-101 0.02231510450780343 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1313012.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.439807399126636 #Duplication Level Percentage of deduplicated Percentage of total 1 73.31773153442546 37.714499890717185 2 14.250383468219137 14.660739619377813 3 5.17809033209767 7.990799081351516 4 2.4378546632622533 5.016110973810921 5 1.3204540226259018 3.3961950301639203 6 0.8353710827724794 2.578279656276973 7 0.4985253701943593 1.7950834318463302 8 0.3556442188820683 1.4635416097525145 9 0.2584753049736239 1.1966327914746362 >10 1.3850295938294035 12.964847819364516 >50 0.10905016823041529 3.813649268967768 >100 0.0510040925663264 4.80762287860593 >500 0.0014913424506289488 0.4889773843504769 >1k 4.474027351886846E-4 0.38004298118485447 >5k 4.474027351886846E-4 1.7329775827545142 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9415 0.7170536141330011 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7898 0.6015177317495956 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5277 0.4019003634391765 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1954 0.14881813722951504 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1809 0.1377748261249707 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 7.616076623823697E-5 0.0 0.0 0.0 0.0 6 7.616076623823697E-5 0.0 0.0 0.0 0.0 7 7.616076623823697E-5 0.0 0.0 0.0 0.0 8 7.616076623823697E-5 0.0 0.0 0.0 0.0 9 7.616076623823697E-5 0.0 0.0 0.0 0.0 10-11 7.616076623823697E-5 0.0 0.0 0.0 0.0 12-13 2.284822987147109E-4 0.0 0.0 0.0 0.0 14-15 3.0464306495294786E-4 0.0 0.0 0.0 0.0 16-17 3.0464306495294786E-4 0.0 0.0 0.0 0.0 18-19 3.427234480720664E-4 0.0 0.0 1.1424114935735546E-4 0.0 20-21 3.8080383119118487E-4 0.0 0.0 2.284822987147109E-4 0.0 22-23 3.8080383119118487E-4 0.0 0.0 3.0464306495294786E-4 0.0 24-25 3.8080383119118487E-4 0.0 0.0 4.569645974294218E-4 0.0 26-27 3.8080383119118487E-4 0.0 0.0 6.092861299058957E-4 0.0 28-29 3.8080383119118487E-4 0.0 0.0 0.0016755368572412134 0.0 30-31 3.8080383119118487E-4 0.0 0.0 0.00426500290934127 0.0 32-33 3.8080383119118487E-4 0.0 0.0 0.009482015396660503 0.0 34-35 4.1888421431030335E-4 0.0 0.0 0.015498715929481223 0.0 36-37 4.569645974294218E-4 0.0 0.0 0.021972381059731365 0.0 38-39 5.331253636676588E-4 0.0 0.0 0.029131493086125643 0.0 40-41 5.331253636676588E-4 0.0 0.0 0.03712837354114053 0.0 42-43 5.331253636676588E-4 0.0 0.0 0.04870481000935254 0.0 44-45 5.331253636676588E-4 0.0 0.0 0.06233758716599696 0.0 46-47 5.331253636676588E-4 0.0 0.0 0.07779822271235906 0.0 48-49 5.331253636676588E-4 0.0 0.0 0.09367774247303147 0.0 50-51 5.331253636676588E-4 0.0 0.0 0.114736194337904 0.0 52-53 5.331253636676588E-4 0.0 0.0 0.16504038043825953 0.0 54-55 5.331253636676588E-4 0.0 0.0 0.2412392270596156 0.0 56-57 5.331253636676588E-4 0.0 0.0 0.30803221905054945 0.0 58-59 5.331253636676588E-4 0.0 0.0 0.40087219309496025 0.0 60-61 5.331253636676588E-4 0.0 0.0 0.5122192333352628 0.0 62-63 5.331253636676588E-4 0.0 0.0 0.5930258063140321 0.0 64-65 5.331253636676588E-4 0.0 0.0 0.6595903160062513 0.0 66-67 6.092861299058957E-4 0.0 0.0 0.8196802466390254 0.0 68-69 6.092861299058957E-4 0.0 0.0 0.9800367399536333 0.0 70-71 7.235272792632513E-4 0.0 0.0 1.0672788976795338 0.0 72-73 7.616076623823697E-4 0.0 0.0 1.2422963384950023 0.0 74-75 7.616076623823697E-4 0.0 0.0 1.4879148096133166 0.0 76-77 7.616076623823697E-4 0.0 0.0 1.742329849232147 0.0 78-79 7.616076623823697E-4 0.0 0.0 1.9853207739152423 0.0 80-81 0.0010662507273353175 0.0 0.0 2.238022196293713 0.0 82-83 0.0010662507273353175 0.0 0.0 2.468218112248784 0.0 84-85 0.001104331110454436 0.0 0.0 2.7518408057199784 0.0 86-87 0.0012185722598117915 0.0 0.0 3.2370991278069052 0.0 88-89 0.0013328134091691469 0.0 0.0 3.7774216838840773 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 6450 0.0 36.398552 1 GTATCAA 9785 0.0 32.929546 1 TATAGCG 80 4.488481E-4 29.693714 5 ATCAACG 12280 0.0 25.998924 3 TATCAAC 12390 0.0 25.921486 2 TCAACGC 12340 0.0 25.872513 4 CAACGCA 12485 0.0 25.572031 5 AACGCAG 12775 0.0 25.022049 6 GAGTACT 8165 0.0 23.269608 12-13 GTACATG 6540 0.0 23.03554 1 TACATGG 6515 0.0 22.898115 2 ACGCAGA 14285 0.0 22.311436 7 CGCAGAG 14215 0.0 22.287647 8 AGTACTT 8460 0.0 22.121323 12-13 GTACTTT 8600 0.0 21.981298 14-15 CGTAAAC 110 0.0028740892 21.595428 3 ACATGGG 6570 0.0 21.54941 3 AGAGTAC 12045 0.0 21.275015 10-11 GCAGAGT 14795 0.0 20.964449 9 TAAGGTG 510 0.0 19.562918 5 >>END_MODULE