FastQCFastQC Report
Thu 26 May 2016
SRR937669_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937669_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219231
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT66900.5487065207495544No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53000.4347002331797666No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33940.27837218705889205No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16620.13631543161222115No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12350.10129335622207768No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50050.036.3815961
GTATCAA74850.032.6481321
ATCAACG89250.026.9455073
TCAACGC89950.026.7908574
CAACGCA90900.026.5119555
TATCAAC91250.026.357092
AACGCAG92600.025.9600186
GTACATG59500.025.1698531
TACATGG58500.024.6187822
GAGTACT53550.023.55044212-13
ACATGGG60150.022.9933073
CGCAGAG104350.022.6726518
ACGCAGA105300.022.6485677
GCAGAGT108050.021.2358639
CATTCCG1805.163598E-621.1137339
CTACACG2509.418727E-920.9137764
GTACTTT59850.020.87432114-15
CATGGGG20350.020.7876534
GTATAGG3001.6552804E-1020.601991
CATGGGA39400.020.2671594
Produced by FastQC (version 0.11.5)