FastQCFastQC Report
Thu 26 May 2016
SRR937669_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937669_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1219231
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89160.7312806186850564No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75840.6220314280066698No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49820.4086182191889806No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19690.16149523757187934No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17000.13943215026520814No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA57800.036.3414961
GTATCAA89800.034.187281
TACCGTG1601.906301E-929.6875657
ATCAACG110400.027.8386123
TCAACGC112300.027.367614
TATCAAC112950.027.2101172
GTACCGT700.00839335827.1418026
CAACGCA113750.027.0605095
AACGCAG115900.026.638316
ACGCAGA131000.023.5324947
GAGTACT75600.023.49872812-13
CGCAGAG130600.023.4590858
GTACATG59450.023.4220051
GCAGAGT134450.022.5046969
TACATGG59800.022.4800682
GTACGTG1703.1357595E-622.3639951
TAAGGTG4900.022.296885
GTACTTT79150.022.26382314-15
GTAAGGT4750.022.000954
GGACCGA2205.6423232E-821.590076
Produced by FastQC (version 0.11.5)